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The map label for this gene is fabI [H]

Identifier: 159184183

GI number: 159184183

Start: 157461

End: 158267

Strand: Reverse

Name: fabI [H]

Synonym: Atu0149

Alternate gene names: 159184183

Gene position: 158267-157461 (Counterclockwise)

Preceding gene: 159184184

Following gene: 159184182

Centisome position: 5.57

GC content: 59.73

Gene sequence:

>807_bases
ATGAACGGCATCATGCAAGGTAAGCGCGGCCTCATCATGGGCGTCGCTAACAATCACTCGATCGCCTGGGGTATTTCCAA
GGCACTTGCGGCACAGGGCGCGGAACTGGCCTTCACCTATCAGGGTGAAGCGCTTGGCAAGCGCGTGAAACCGCTCGCCG
CGGAACTGGGCTCGGACTTCATCGTACCTTGCGATGTCGAGGATATCGCTTCTGTGGATGCGCTTTTTGCGACGATCCGC
GAAAAATGGGGCGCGCTGGATTTCGTCGTGCACGCCATCGGCTTTTCCGACAAGAACGAGCTGAAGGGTCTCTACGCCAA
CACGACGCGCGAGAATTTCAGCCGCACCATGGTCATTTCCTGTTTCTCCTTCACGGAAATCGCCAAGCGCGCCGCTGAAC
TGATGACCAATGGCGGCTCCATGCTGACGCTGACCTATAATGGTTCGCAGCGCGTCATTCCAAACTACAACGTCATGGGC
GTCGCCAAGGCGGCTCTCGAGGCATCCGTGCGTTATCTCGCCGCCGATTACGGCCCGCGCGATATCCGCGTCAACGCCAT
CTCGGCCGGCCCGGTCCGCACGCTGGCGGGTGCCGGCATTTCGGATGCGCGCGCAATTTACGCGTGGAACCAGAAAAACG
CACCGTTGCGGCGAACGGCTGACATCGACGACATCGGCGGCTCCGCACTTTACCTGCTGTCCAGCCTGTCGCGCGGCGTG
ACCGGCGAATGTCATTACGTGGATTGCGGTTACAATATCACCTCGACGCCCACGCTTGAGGTTCTGTCCAAGGCAGACGC
GGAATAA

Upstream 100 bases:

>100_bases
CATCGACGACGCCAAGATCGACACCATCCTTTCCAATTCCTTCGGCTTCGGCGGCACCAACGCCACGCTCGTCTTCCAGC
GTCATAACGGATAATTGCCC

Downstream 100 bases:

>100_bases
GTCGTCTTCTGATATTCGACAAAAGCCCGGCCATGTGCCGGGCTTTTTTATGCGCCGTATCAATTTTGGAAATCACTAAA
GTATAGTTTATCGAAGTAAC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGEALGKRVKPLAAELGSDFIVPCDVEDIASVDALFATIR
EKWGALDFVVHAIGFSDKNELKGLYANTTRENFSRTMVISCFSFTEIAKRAAELMTNGGSMLTLTYNGSQRVIPNYNVMG
VAKAALEASVRYLAADYGPRDIRVNAISAGPVRTLAGAGISDARAIYAWNQKNAPLRRTADIDDIGGSALYLLSSLSRGV
TGECHYVDCGYNITSTPTLEVLSKADAE

Sequences:

>Translated_268_residues
MNGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGEALGKRVKPLAAELGSDFIVPCDVEDIASVDALFATIR
EKWGALDFVVHAIGFSDKNELKGLYANTTRENFSRTMVISCFSFTEIAKRAAELMTNGGSMLTLTYNGSQRVIPNYNVMG
VAKAALEASVRYLAADYGPRDIRVNAISAGPVRTLAGAGISDARAIYAWNQKNAPLRRTADIDDIGGSALYLLSSLSRGV
TGECHYVDCGYNITSTPTLEVLSKADAE
>Mature_268_residues
MNGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGEALGKRVKPLAAELGSDFIVPCDVEDIASVDALFATIR
EKWGALDFVVHAIGFSDKNELKGLYANTTRENFSRTMVISCFSFTEIAKRAAELMTNGGSMLTLTYNGSQRVIPNYNVMG
VAKAALEASVRYLAADYGPRDIRVNAISAGPVRTLAGAGISDARAIYAWNQKNAPLRRTADIDDIGGSALYLLSSLSRGV
TGECHYVDCGYNITSTPTLEVLSKADAE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=260, Percent_Identity=49.2307692307692, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI2367365, Length=260, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=26.4822134387352, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=26.615969581749, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28641; Mature: 28641

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGEALGKRVKPLAAELGSDF
CCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCC
IVPCDVEDIASVDALFATIREKWGALDFVVHAIGFSDKNELKGLYANTTRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHCCCEEECCCHHHCCEEEEEE
CFSFTEIAKRAAELMTNGGSMLTLTYNGSQRVIPNYNVMGVAKAALEASVRYLAADYGPR
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DIRVNAISAGPVRTLAGAGISDARAIYAWNQKNAPLRRTADIDDIGGSALYLLSSLSRGV
EEEEEEECCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
TGECHYVDCGYNITSTPTLEVLSKADAE
CCCEEEEECCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGEALGKRVKPLAAELGSDF
CCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCC
IVPCDVEDIASVDALFATIREKWGALDFVVHAIGFSDKNELKGLYANTTRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHCCCEEECCCHHHCCEEEEEE
CFSFTEIAKRAAELMTNGGSMLTLTYNGSQRVIPNYNVMGVAKAALEASVRYLAADYGPR
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DIRVNAISAGPVRTLAGAGISDARAIYAWNQKNAPLRRTADIDDIGGSALYLLSSLSRGV
EEEEEEECCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
TGECHYVDCGYNITSTPTLEVLSKADAE
CCCEEEEECCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]