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The map label for this gene is actS [H]

Identifier: 159184141

GI number: 159184141

Start: 52255

End: 53562

Strand: Reverse

Name: actS [H]

Synonym: Atu0051

Alternate gene names: 159184141

Gene position: 53562-52255 (Counterclockwise)

Preceding gene: 159184142

Following gene: 15887408

Centisome position: 1.88

GC content: 60.7

Gene sequence:

>1308_bases
GTGGCTGGCCATCCGATCGAAACCACCAGGCTTGGCCGCGCCAGCCGCAGGATAAGGTTGCAGACTATCATCTGGCTGCG
CTGGCTGGCGGTGGCCGGACAGACGGCGACGATCCTGTTTGTCGCTTTCGCGCTGAATTTTCCCCTGCCGCTTCTCGCCT
GCGGACTTCTGATCGCGGCGCTGGCGCTTGCCAATCTGTTTCTCACCGCCCGTTTTCCGTCCACCTATCGTCTGGAGCCG
TGGGAAGCGACATTGCTGCTCGCCTTCGACCTGCTGCAACTGACGGCACTGATCTATATTACCGGTGGACTTTCCAATCC
CTTCGCGCCGCTGATCTGCGTGCAGGTCATCATCGCCTTTGCCTCGCAGCCGCTGAAGCATTCGCTCATCCTGCTCGGCT
TTGCCGTCATCTGCTCGACGGCGATCGCCTTTTCGCCTTTTCCTGTTCCCTGGTACCCCGACGAGGTTTTGCCGATCCAG
CTTCTGCTGCATGTCGGCACCTGGGTTGCGATCATCGCTACTACGTCTTTCGCGGCCTTTTATGCCTATCGCGTTTCACA
TGAGGCCAATCAACTGGCTGATGCGCTGGCGGCAACGGAGCTGGTGCTGGAGCGGGAAAAACACCTTTCCCAGCTCGACG
GGCTGGCGGCAGCGGCAGCGCATGAGCTCGGCACGCCGCTTGCGACAATCAGCGTCGTCGCCAAGGAAATGGAACGGGAA
CTGGGGCATGATGAGAAATACGGCGAAGACATCGCGCTGCTGCGCAGCCAGAGCGAAAGATGCCGCGATATTCTGCGCCG
GCTCACCTCGCTTTCCGCCGACAACGAGGAGCATATGAGCCGCCTTCCACTTTCCTCGCTGATCGAGGAGGTGATGGCGC
CGCATCGCGAGTTTGGCATTCGCCTGGAACTGGTGGAAAAGACTGGCCGTGCCGAGGAGCCGGTCGGTATCCGCAACGCC
GGCATTATCTACGGACTGGGCAACCTCATCGAAAATGCCGTGGACTATGCGCGTGAGAAGGTGACCGTGACGGTGGATCA
CGATGCCCAGAACGTCGCCGTCACCATAGAGGATGACGGTCCGGGTTATGCCCATGACGTGCTTGCCCGTATCGGCGAGC
CCTATGTGACCGAACGTCACAATGATACGCGCGCCGGTGGGCTCGGGCTCGGGCTTTTCATCGCCAAGACCTTGCTGGAG
CGTTCCGGCGCGGCCGTCACCTTTGAGAATCGCGCGTCAAGCAAAAGCGGGGCGCGGGTCACAATCGTCTGGCCTCGGGC
GCTGATGGACGATAAAAGAAGCCGTTGA

Upstream 100 bases:

>100_bases
CGCGCCATCCCTGGCCGGAGGATCCGGCCTCGGCCCTGCCGACCAGCCGTGCGAAACCGCGCGGGACGTGACGATGATGA
AAAGGGTAAAGGAGAAGACG

Downstream 100 bases:

>100_bases
CTTTACCATCCACGTTGAAGCGGCGATAAGAAGAAGAAAATCAAGCCGATGAAGTTGCCGGAAGGCCGGAAACATGAAGA
TTGAAGACCAGACCCCAGCC

Product: two component sensor kinase

Products: NA

Alternate protein names: Protein prrB [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAGHPIETTRLGRASRRIRLQTIIWLRWLAVAGQTATILFVAFALNFPLPLLACGLLIAALALANLFLTARFPSTYRLEP
WEATLLLAFDLLQLTALIYITGGLSNPFAPLICVQVIIAFASQPLKHSLILLGFAVICSTAIAFSPFPVPWYPDEVLPIQ
LLLHVGTWVAIIATTSFAAFYAYRVSHEANQLADALAATELVLEREKHLSQLDGLAAAAAHELGTPLATISVVAKEMERE
LGHDEKYGEDIALLRSQSERCRDILRRLTSLSADNEEHMSRLPLSSLIEEVMAPHREFGIRLELVEKTGRAEEPVGIRNA
GIIYGLGNLIENAVDYAREKVTVTVDHDAQNVAVTIEDDGPGYAHDVLARIGEPYVTERHNDTRAGGLGLGLFIAKTLLE
RSGAAVTFENRASSKSGARVTIVWPRALMDDKRSR

Sequences:

>Translated_435_residues
MAGHPIETTRLGRASRRIRLQTIIWLRWLAVAGQTATILFVAFALNFPLPLLACGLLIAALALANLFLTARFPSTYRLEP
WEATLLLAFDLLQLTALIYITGGLSNPFAPLICVQVIIAFASQPLKHSLILLGFAVICSTAIAFSPFPVPWYPDEVLPIQ
LLLHVGTWVAIIATTSFAAFYAYRVSHEANQLADALAATELVLEREKHLSQLDGLAAAAAHELGTPLATISVVAKEMERE
LGHDEKYGEDIALLRSQSERCRDILRRLTSLSADNEEHMSRLPLSSLIEEVMAPHREFGIRLELVEKTGRAEEPVGIRNA
GIIYGLGNLIENAVDYAREKVTVTVDHDAQNVAVTIEDDGPGYAHDVLARIGEPYVTERHNDTRAGGLGLGLFIAKTLLE
RSGAAVTFENRASSKSGARVTIVWPRALMDDKRSR
>Mature_434_residues
AGHPIETTRLGRASRRIRLQTIIWLRWLAVAGQTATILFVAFALNFPLPLLACGLLIAALALANLFLTARFPSTYRLEPW
EATLLLAFDLLQLTALIYITGGLSNPFAPLICVQVIIAFASQPLKHSLILLGFAVICSTAIAFSPFPVPWYPDEVLPIQL
LLHVGTWVAIIATTSFAAFYAYRVSHEANQLADALAATELVLEREKHLSQLDGLAAAAAHELGTPLATISVVAKEMEREL
GHDEKYGEDIALLRSQSERCRDILRRLTSLSADNEEHMSRLPLSSLIEEVMAPHREFGIRLELVEKTGRAEEPVGIRNAG
IIYGLGNLIENAVDYAREKVTVTVDHDAQNVAVTIEDDGPGYAHDVLARIGEPYVTERHNDTRAGGLGLGLFIAKTLLER
SGAAVTFENRASSKSGARVTIVWPRALMDDKRSR

Specific function: Member of the two-component regulatory system RegB/RegA. Involved in the positive regulation of photosynthesis gene expression in response to anaerobiosis. Also involved in positive regulation of the cbbI and cbbII Calvin cycle CO2 fixation operons, as we

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=318, Percent_Identity=27.6729559748428, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47682; Mature: 47550

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHPIETTRLGRASRRIRLQTIIWLRWLAVAGQTATILFVAFALNFPLPLLACGLLIAA
CCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LALANLFLTARFPSTYRLEPWEATLLLAFDLLQLTALIYITGGLSNPFAPLICVQVIIAF
HHHHHHHHHEECCCCEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
ASQPLKHSLILLGFAVICSTAIAFSPFPVPWYPDEVLPIQLLLHVGTWVAIIATTSFAAF
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YAYRVSHEANQLADALAATELVLEREKHLSQLDGLAAAAAHELGTPLATISVVAKEMERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGHDEKYGEDIALLRSQSERCRDILRRLTSLSADNEEHMSRLPLSSLIEEVMAPHREFGI
CCCCCHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCHHHHCC
RLELVEKTGRAEEPVGIRNAGIIYGLGNLIENAVDYAREKVTVTVDHDAQNVAVTIEDDG
EEEEEHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEECCC
PGYAHDVLARIGEPYVTERHNDTRAGGLGLGLFIAKTLLERSGAAVTFENRASSKSGARV
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEE
TIVWPRALMDDKRSR
EEEECHHHHCCCCCC
>Mature Secondary Structure 
AGHPIETTRLGRASRRIRLQTIIWLRWLAVAGQTATILFVAFALNFPLPLLACGLLIAA
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LALANLFLTARFPSTYRLEPWEATLLLAFDLLQLTALIYITGGLSNPFAPLICVQVIIAF
HHHHHHHHHEECCCCEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
ASQPLKHSLILLGFAVICSTAIAFSPFPVPWYPDEVLPIQLLLHVGTWVAIIATTSFAAF
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YAYRVSHEANQLADALAATELVLEREKHLSQLDGLAAAAAHELGTPLATISVVAKEMERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGHDEKYGEDIALLRSQSERCRDILRRLTSLSADNEEHMSRLPLSSLIEEVMAPHREFGI
CCCCCHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCHHHHCC
RLELVEKTGRAEEPVGIRNAGIIYGLGNLIENAVDYAREKVTVTVDHDAQNVAVTIEDDG
EEEEEHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEECCC
PGYAHDVLARIGEPYVTERHNDTRAGGLGLGLFIAKTLLERSGAAVTFENRASSKSGARV
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEE
TIVWPRALMDDKRSR
EEEECHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751278; 10358089 [H]