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The map label for this gene is pckA

Identifier: 159184132

GI number: 159184132

Start: 37589

End: 39199

Strand: Direct

Name: pckA

Synonym: Atu0035

Alternate gene names: 159184132

Gene position: 37589-39199 (Clockwise)

Preceding gene: 15887380

Following gene: 159184133

Centisome position: 1.32

GC content: 60.77

Gene sequence:

>1611_bases
ATGAACGAGCTTGGGGTTCATAATCCTGCCAATGGAGTTGCGGAACTCGGACTGGGCGAGGCCTCCCGCGTCTTTTATAA
CCTCAATGAGAGCGAATTATACGAACACGCGATCCGTAACGGCGAAGCCGAACTGACCATCGACGGCGCGCTGCGCGCCG
TCACCGGCCAGCATACGGGCCGCTCGCCGAAGGATAAATTCGTCGTTCGTGATGCTTCCACCGAAAACACCATCTGGTGG
GACAACAACAAGCCGCTGTCGCCGGAAAATTTCGAGCTGCTGCGTCAGGACATGCTGGCCCATGCCGCCGGCAAGACGCT
TTACGTGCAGGATCTGATCGGCGGTGCCGATGAGGAAAATGCGCTGCCGACCCGCGTCGTCACCGAGCTTGCCTGGCATT
CGCTGTTCATCCGCAATCTACTGATCCGGCCGAAGCGGGAGACGCTTTCGGGCTTCAGCCAGAAGCTCACCATCATCAAC
CTGCCAAGCTTCAAGGCTGATCCGGCCCGTCATGGCGTTCGCTCGGAAACGGTCATTGCCTGCGATCTGACGAATGGCCT
CGTGCTCATCGGCGGCACGTCCTATGCCGGTGAAAACAAGAAGTCGGTCTTCACGGTGCTGAACTACCTGCTGCCGGCCA
AGGGCGTGATGCCGATGCATTGCTCGGCCAATGTCGGTCCTGAGGGTGATTCCGCCGTGTTCTTCGGCCTCTCCGGCACC
GGCAAGACCACGCTCTCCGCCGACCCGGCACGCACGCTGATCGGCGATGACGAACATGGCTGGGGCGAACACGGCATCTT
CAACTTCGAGGGCGGCTGTTATGCCAAGGCGATCAAGCTTTCGTCCGAGGCCGAGCCGGAAATCTACGCCGCGACCAATC
GTTTCGGCACGGTTCTCGAAAACGTCGTGCTGGATGAAAGCCGGGTTCCTGATTTCAACGACAACTCGCTGACGGAAAAC
ACCCGCAGCGCCTATCCGCTGCACTTCATTCCGAATGCATCGGAAACGGGCATTGCCGGCCATCCGAAGACGATCATCAT
GCTGACGGCGGATGCCTTCGGCGTGCTGCCGCCGATCGCGCGTCTGACGCCGGAACAGGCCATGTACCACTTCCTCTCCG
GTTACACCGCCAAGGTTGCCGGCACGGAAAAGGGCGTGACCGAGCCGGAAGCCACATTCTCCACCTGCTTCGGCGCACCC
TTCATGCCGCGTCATCCGGCGGAATACGGCAACCTGCTGCGTGAGCTGATCGGCAAGCACGGCGTCGATTGCTGGCTGGT
CAATACCGGCTGGACCGGCGGCGCCTATGGCATCGGCAAGCGTATGCCCATCAAGGCGACGCGCGCGCTTCTGACCGCCG
CACTTACCGGCGACCTGAAGAATGCGCAATTCCGCACCGATGCGAATTTCGGTTTCGCCGTACCGCTGTCGCTCGACGGC
GTCGATGGTGCGATCCTTGATCCACGTTCCACCTGGGCCGATGGCGCAGCTTACGACGCGCAGGCGAAGAAGCTGGTATC
GATGTTCGTTTCGAACTTCACCAAGTTCGAAGATCACGTCGACAGCAAGGTTCGCGACGCAGCCCCGGGCCTTCTGCTCG
CGGCTGAATGA

Upstream 100 bases:

>100_bases
TACTAAGTTTTTTAAAGTCAGGCTCTTGCGTTTCCAAAAGCTGGCACGTATTTTCCGTGAAGATTTCAACGGCCTTATCG
TCATTTACAAGGAACGCGTC

Downstream 100 bases:

>100_bases
ATATAAAGTCTTTCGTGAGGAGGAGGGAACCCGGCCCAGTGCCGGGTTTCTTTTTGCCGGGTTCTTTCATTTTGTCCGCT
GTTTTCATTTTTTGCGTTCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MNELGVHNPANGVAELGLGEASRVFYNLNESELYEHAIRNGEAELTIDGALRAVTGQHTGRSPKDKFVVRDASTENTIWW
DNNKPLSPENFELLRQDMLAHAAGKTLYVQDLIGGADEENALPTRVVTELAWHSLFIRNLLIRPKRETLSGFSQKLTIIN
LPSFKADPARHGVRSETVIACDLTNGLVLIGGTSYAGENKKSVFTVLNYLLPAKGVMPMHCSANVGPEGDSAVFFGLSGT
GKTTLSADPARTLIGDDEHGWGEHGIFNFEGGCYAKAIKLSSEAEPEIYAATNRFGTVLENVVLDESRVPDFNDNSLTEN
TRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIARLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPAEYGNLLRELIGKHGVDCWLVNTGWTGGAYGIGKRMPIKATRALLTAALTGDLKNAQFRTDANFGFAVPLSLDG
VDGAILDPRSTWADGAAYDAQAKKLVSMFVSNFTKFEDHVDSKVRDAAPGLLLAAE

Sequences:

>Translated_536_residues
MNELGVHNPANGVAELGLGEASRVFYNLNESELYEHAIRNGEAELTIDGALRAVTGQHTGRSPKDKFVVRDASTENTIWW
DNNKPLSPENFELLRQDMLAHAAGKTLYVQDLIGGADEENALPTRVVTELAWHSLFIRNLLIRPKRETLSGFSQKLTIIN
LPSFKADPARHGVRSETVIACDLTNGLVLIGGTSYAGENKKSVFTVLNYLLPAKGVMPMHCSANVGPEGDSAVFFGLSGT
GKTTLSADPARTLIGDDEHGWGEHGIFNFEGGCYAKAIKLSSEAEPEIYAATNRFGTVLENVVLDESRVPDFNDNSLTEN
TRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIARLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPAEYGNLLRELIGKHGVDCWLVNTGWTGGAYGIGKRMPIKATRALLTAALTGDLKNAQFRTDANFGFAVPLSLDG
VDGAILDPRSTWADGAAYDAQAKKLVSMFVSNFTKFEDHVDSKVRDAAPGLLLAAE
>Mature_536_residues
MNELGVHNPANGVAELGLGEASRVFYNLNESELYEHAIRNGEAELTIDGALRAVTGQHTGRSPKDKFVVRDASTENTIWW
DNNKPLSPENFELLRQDMLAHAAGKTLYVQDLIGGADEENALPTRVVTELAWHSLFIRNLLIRPKRETLSGFSQKLTIIN
LPSFKADPARHGVRSETVIACDLTNGLVLIGGTSYAGENKKSVFTVLNYLLPAKGVMPMHCSANVGPEGDSAVFFGLSGT
GKTTLSADPARTLIGDDEHGWGEHGIFNFEGGCYAKAIKLSSEAEPEIYAATNRFGTVLENVVLDESRVPDFNDNSLTEN
TRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIARLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPAEYGNLLRELIGKHGVDCWLVNTGWTGGAYGIGKRMPIKATRALLTAALTGDLKNAQFRTDANFGFAVPLSLDG
VDGAILDPRSTWADGAAYDAQAKKLVSMFVSNFTKFEDHVDSKVRDAAPGLLLAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=520, Percent_Identity=48.8461538461538, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322950, Length=525, Percent_Identity=45.7142857142857, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_AGRT5 (Q8UJ94)

Other databases:

- EMBL:   AE007869
- PIR:   AD2581
- PIR:   B97363
- RefSeq:   NP_353074.2
- ProteinModelPortal:   Q8UJ94
- SMR:   Q8UJ94
- STRING:   Q8UJ94
- GeneID:   1132073
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0035
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q8UJ94
- ProtClustDB:   PRK09344
- BioCyc:   ATUM176299-1:ATU0035-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 57883; Mature: 57883

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELGVHNPANGVAELGLGEASRVFYNLNESELYEHAIRNGEAELTIDGALRAVTGQHTG
CCCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEECCHHEEECCCCCC
RSPKDKFVVRDASTENTIWWDNNKPLSPENFELLRQDMLAHAAGKTLYVQDLIGGADEEN
CCCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCC
ALPTRVVTELAWHSLFIRNLLIRPKRETLSGFSQKLTIINLPSFKADPARHGVRSETVIA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEEEEECCCCCCCHHHCCCCCCEEEE
CDLTNGLVLIGGTSYAGENKKSVFTVLNYLLPAKGVMPMHCSANVGPEGDSAVFFGLSGT
EECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPARTLIGDDEHGWGEHGIFNFEGGCYAKAIKLSSEAEPEIYAATNRFGTVLE
CCCEECCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHH
NVVLDESRVPDFNDNSLTENTRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEECCHHCCCCCHH
RLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPAEYGNLLRELIGKH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVDCWLVNTGWTGGAYGIGKRMPIKATRALLTAALTGDLKNAQFRTDANFGFAVPLSLDG
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECC
VDGAILDPRSTWADGAAYDAQAKKLVSMFVSNFTKFEDHVDSKVRDAAPGLLLAAE
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MNELGVHNPANGVAELGLGEASRVFYNLNESELYEHAIRNGEAELTIDGALRAVTGQHTG
CCCCCCCCCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEECCHHEEECCCCCC
RSPKDKFVVRDASTENTIWWDNNKPLSPENFELLRQDMLAHAAGKTLYVQDLIGGADEEN
CCCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCC
ALPTRVVTELAWHSLFIRNLLIRPKRETLSGFSQKLTIINLPSFKADPARHGVRSETVIA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEEEEECCCCCCCHHHCCCCCCEEEE
CDLTNGLVLIGGTSYAGENKKSVFTVLNYLLPAKGVMPMHCSANVGPEGDSAVFFGLSGT
EECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPARTLIGDDEHGWGEHGIFNFEGGCYAKAIKLSSEAEPEIYAATNRFGTVLE
CCCEECCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHH
NVVLDESRVPDFNDNSLTENTRSAYPLHFIPNASETGIAGHPKTIIMLTADAFGVLPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEECCHHCCCCCHH
RLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPAEYGNLLRELIGKH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVDCWLVNTGWTGGAYGIGKRMPIKATRALLTAALTGDLKNAQFRTDANFGFAVPLSLDG
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECC
VDGAILDPRSTWADGAAYDAQAKKLVSMFVSNFTKFEDHVDSKVRDAAPGLLLAAE
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194