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The map label for this gene is trpB

Identifier: 159184125

GI number: 159184125

Start: 15139

End: 16359

Strand: Direct

Name: trpB

Synonym: Atu0018

Alternate gene names: 159184125

Gene position: 15139-16359 (Clockwise)

Preceding gene: 15887376

Following gene: 159184126

Centisome position: 0.53

GC content: 59.38

Gene sequence:

>1221_bases
GTGAACGACGCGCCAACACCCAATTCTTTTCGTGCTGGTCCCGATGAGGACGGACGCTTCGGCATTTACGGAGGACGCTT
CGTTGCCGAAACGCTGATGCCGCTTATCCTGGATTTGCAGGCGGAATGGGATAAGGCCAAGAGCGATCCCGAGTTTCAGG
CCGAACTAAAATATCTCGGCACGCATTACACCGGCCGCCCGAGCCCGCTTTATTTCGCGGAGCGTCTCACGGCGGAACTT
GGCGGCGCGAAGATTTATTTCAAGCGCGAAGAGTTGAACCATACTGGCTCGCACAAGATCAACAATTGCCTCGGCCAGAT
CCTGCTTGCCAAGCGCATGGGCAAGACCCGCATCATTGCCGAAACCGGTGCGGGCCAGCACGGCGTTGCCTCGGCCACGG
TTGCTGCACGTTTCGGCCTGCCTTGCGTGGTTTATATGGGCGCCACTGATGTCGCCCGTCAGGCCCCGAACGTATTTCGC
ATGAAGCTTCTCGGCGCCGAGGTAAAGCCGGTCACGGCCGGCCATGGCACGCTCAAGGACGCCATGAACGAGGCACTGCG
TGACTGGGTCACCAATGTCGACGATACCTATTACCTGATTGGCACCGCAGCCGGCCCGCATCCCTATCCGGAAATGGTCC
GTGATTTCCAGGCGGTTATCGGCGAGGAAGCCAAGGCGCAGATGCTGGAGGCGGAAGGCCGCCTGCCGGATATGATCATT
GCCGCTGTCGGCGGCGGGTCGAACGCCATCGGCATCTTCCATCCGTTCCTCGATGACAAGAATGTCCGTATCGTCGGCGT
CGAAGCCGGCGGCAAGGGCCTGTCTGGCGAAGAACACTGCGCCTCGATCACAGCCGGTTCACCGGGCGTGCTGCATGGCA
ACCGCACCTATCTGCTTCAGGACGGTGATGGCCAGATCAAGGAAGGTCATTCGATCTCCGCAGGCCTCGATTATCCCGGC
ATCGGGCCAGAGCATTCCTGGCTGAACGATATCGGCCGCGTCGAATATGTGCCGATCATGGACCATGAGGCATTGGACGC
CTTCCAGATGTTGACCCGCCTCGAAGGGATCATTCCGGCTCTGGAGCCAAGCCACGCGCTGGCCGAAGTCATCAAGCGCG
CGCCGAAAATGGGCAAGGATGAAATCATCCTGATGAACCTCTCCGGCCGTGGCGACAAAGATGTCCACACCGTCAGCAAG
TTCCTTGGAATGGATGTATAA

Upstream 100 bases:

>100_bases
TCTGGCGTCGAAAGCGCGCCGGGCGTCAAGGATTTGTCCAAGATTGATGCTTTTTTCGATGTGGTGAATGATTGGTCGAA
AGGCCCAAAGGGAGCTTGAA

Downstream 100 bases:

>100_bases
GATCATGACTGTACGTATGGACAAGCGCTTTGCCGATCTGCGGGCTGAAAATCGCCCCGCCCTGATCACCTATTTCATGG
GCGGCGACCCGGATTTCCAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNDAPTPNSFRAGPDEDGRFGIYGGRFVAETLMPLILDLQAEWDKAKSDPEFQAELKYLGTHYTGRPSPLYFAERLTAEL
GGAKIYFKREELNHTGSHKINNCLGQILLAKRMGKTRIIAETGAGQHGVASATVAARFGLPCVVYMGATDVARQAPNVFR
MKLLGAEVKPVTAGHGTLKDAMNEALRDWVTNVDDTYYLIGTAAGPHPYPEMVRDFQAVIGEEAKAQMLEAEGRLPDMII
AAVGGGSNAIGIFHPFLDDKNVRIVGVEAGGKGLSGEEHCASITAGSPGVLHGNRTYLLQDGDGQIKEGHSISAGLDYPG
IGPEHSWLNDIGRVEYVPIMDHEALDAFQMLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSK
FLGMDV

Sequences:

>Translated_406_residues
MNDAPTPNSFRAGPDEDGRFGIYGGRFVAETLMPLILDLQAEWDKAKSDPEFQAELKYLGTHYTGRPSPLYFAERLTAEL
GGAKIYFKREELNHTGSHKINNCLGQILLAKRMGKTRIIAETGAGQHGVASATVAARFGLPCVVYMGATDVARQAPNVFR
MKLLGAEVKPVTAGHGTLKDAMNEALRDWVTNVDDTYYLIGTAAGPHPYPEMVRDFQAVIGEEAKAQMLEAEGRLPDMII
AAVGGGSNAIGIFHPFLDDKNVRIVGVEAGGKGLSGEEHCASITAGSPGVLHGNRTYLLQDGDGQIKEGHSISAGLDYPG
IGPEHSWLNDIGRVEYVPIMDHEALDAFQMLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSK
FLGMDV
>Mature_406_residues
MNDAPTPNSFRAGPDEDGRFGIYGGRFVAETLMPLILDLQAEWDKAKSDPEFQAELKYLGTHYTGRPSPLYFAERLTAEL
GGAKIYFKREELNHTGSHKINNCLGQILLAKRMGKTRIIAETGAGQHGVASATVAARFGLPCVVYMGATDVARQAPNVFR
MKLLGAEVKPVTAGHGTLKDAMNEALRDWVTNVDDTYYLIGTAAGPHPYPEMVRDFQAVIGEEAKAQMLEAEGRLPDMII
AAVGGGSNAIGIFHPFLDDKNVRIVGVEAGGKGLSGEEHCASITAGSPGVLHGNRTYLLQDGDGQIKEGHSISAGLDYPG
IGPEHSWLNDIGRVEYVPIMDHEALDAFQMLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSK
FLGMDV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=55.7291666666667, Blast_Score=430, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=55.3246753246753, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_AGRT5 (Q8UJB0)

Other databases:

- EMBL:   AE007869
- PIR:   AC2579
- PIR:   B97361
- RefSeq:   NP_353058.2
- ProteinModelPortal:   Q8UJB0
- SMR:   Q8UJB0
- STRING:   Q8UJB0
- GeneID:   1132056
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0018
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q8UJB0
- ProtClustDB:   PRK04346
- BioCyc:   ATUM176299-1:ATU0018-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43829; Mature: 43829

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAPTPNSFRAGPDEDGRFGIYGGRFVAETLMPLILDLQAEWDKAKSDPEFQAELKYLG
CCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
THYTGRPSPLYFAERLTAELGGAKIYFKREELNHTGSHKINNCLGQILLAKRMGKTRIIA
CCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGVASATVAARFGLPCVVYMGATDVARQAPNVFRMKLLGAEVKPVTAGHGTLKD
ECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEHHHHCCCCCEEECCCCHHHH
AMNEALRDWVTNVDDTYYLIGTAAGPHPYPEMVRDFQAVIGEEAKAQMLEAEGRLPDMII
HHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEE
AAVGGGSNAIGIFHPFLDDKNVRIVGVEAGGKGLSGEEHCASITAGSPGVLHGNRTYLLQ
EEECCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCHHHEEECCCCCCEEECCEEEEEE
DGDGQIKEGHSISAGLDYPGIGPEHSWLNDIGRVEYVPIMDHEALDAFQMLTRLEGIIPA
CCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
LEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSKFLGMDV
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNDAPTPNSFRAGPDEDGRFGIYGGRFVAETLMPLILDLQAEWDKAKSDPEFQAELKYLG
CCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
THYTGRPSPLYFAERLTAELGGAKIYFKREELNHTGSHKINNCLGQILLAKRMGKTRIIA
CCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGVASATVAARFGLPCVVYMGATDVARQAPNVFRMKLLGAEVKPVTAGHGTLKD
ECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEHHHHCCCCCEEECCCCHHHH
AMNEALRDWVTNVDDTYYLIGTAAGPHPYPEMVRDFQAVIGEEAKAQMLEAEGRLPDMII
HHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEE
AAVGGGSNAIGIFHPFLDDKNVRIVGVEAGGKGLSGEEHCASITAGSPGVLHGNRTYLLQ
EEECCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCHHHEEECCCCCCEEECCEEEEEE
DGDGQIKEGHSISAGLDYPGIGPEHSWLNDIGRVEYVPIMDHEALDAFQMLTRLEGIIPA
CCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
LEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDVHTVSKFLGMDV
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194