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The map label for this gene is gyrB [H]

Identifier: 159184123

GI number: 159184123

Start: 8064

End: 10499

Strand: Direct

Name: gyrB [H]

Synonym: Atu0012

Alternate gene names: 159184123

Gene position: 8064-10499 (Clockwise)

Preceding gene: 15887370

Following gene: 15887373

Centisome position: 0.28

GC content: 60.3

Gene sequence:

>2436_bases
ATGACCGAAACATCGTCAAGCGAAGTCGGCGCGAACGCGGAATATGGAGCCGATTCGATCAAGGTCCTCAAGGGCCTTGA
TGCCGTGCGCAAACGGCCCGGCATGTATATCGGCGATACCGATGACGGTTCCGGCCTGCATCACATGGTCTACGAAGTCG
TCGACAACGCCATCGATGAGGCGCTCGCCGGCCATGCCGATCTGGTAACAGTGACGCTGAATGCCGACGGTTCCGTGACG
GTGACCGATAACGGACGCGGCATTCCGACCGACATCCATTCCTCCGAAGGCGTTTCCGCCGCCGAAGTCATCATGACCCA
GCTTCATGCGGGCGGCAAATTCGACCAGAACTCCTATAAGGTTTCCGGTGGTCTGCACGGCGTTGGTGTTTCGGTGGTGA
ACGCTCTTTCCGTCTGGCTGAAGCTCAGAATCCGCCGCAATGGCAAACTGCACGAAATCGGCTTCACCCACGGCGTTGCC
GATGCGCCGCTGTCGGTCATCGGCGAATATGAAGGCCGGTCCGGCACGGAAGTCACCTTCCTGGCCAGCCCGCAAACCTT
CACCATGACGGATTATGATTACGGCACGCTGGAGCATCGCCTGCGTGAACTCGCGTTCCTGAATTCCGGCGTCCGTATCC
TGCTCACCGACAAACGCCACTCGGACGTCAAGCAGCAGGAATTGCTGTATGACGGCGGCCTCGAAGCCTTCGTCCGTTAT
CTCGACCGCGCCAAGAAGCCGCTGGTGGACAAACCCATCGCCATTCACAGCGAGAAGGACGGTATCACCGTCGAAGTCGC
GCTGTGGTGGAACGACAGCTACCACGAGAATGTGCTCTGCTTCACCAACAACATTCCCCAGCGCGATGGCGGCACCCATA
TGGCCGGTTTCCGCGCGGCGCTTACCCGTCAGGTCACCTCCTATGCCGATACATCCGGCATCATGAAAAGAGAAAAGGTG
AGCCTGCAGGGTGAGGACTGCCGTGAAGGCCTGACGGCCATTCTTTCGGTCAAGGTTCCCGATCCCAAGTTTTCCTCGCA
GACGAAGGACAAGCTCGTTTCGTCGGAAGTGCGACCGGTCGTGGAAAGCCTCGTTAACGAAGCGCTCTCCACCTGGCTCG
AAGAGCACCCTTCCGAAGCGAAGATACTCATCGGCAAGGTTGTGGAAGCGGCCGTCGCCCGTGAAGCCGCCCGCAAGGCT
CGCGAATTGACGCGCCGCAAAGGCGCGCTCGATATCGCTTCTCTGCCCGGCAAGCTTGCCGATTGCTCGGAGCGCGATCC
GGCCAAATCCGAACTCTTTCTCGTCGAGGGCGACTCCGCCGGTGGTTCCGCCAAGCAAGGCCGCTCGCGTGAAACGCAGG
CCATCCTGCCGCTTCGCGGGAAGATTCTCAACGTGGAGCGCGCCCGTTTCGATAAGATGTTGTCCAGCCAGGAAATCGGC
ACGCTGATTACCGCGCTCGGCACATCGATCGGCAAGGACGAGTTCAATGCCGACAAGCTGCGTTACCACAAGATCATCAT
CATGACGGATGCTGACGTCGACGGCGCCCACATCCGTACCCTACTATTGACCTTCTTCTTCCGCCAGATGCCGGAACTGA
TCGAGCGCGGCCATCTCTATATCGCCCAGCCGCCACTTTATAAGGTGACGCGCGGTAAATCCGTGCAGTACCTGAAGGAT
GAGAAGGCGCTGGAAGAATACCTGATCTCCATGGGTATCGAGGAAGCGTCACTGACGCTCGGCACCGGTGAGGTCCGAGT
CGGCGCCGATCTGCGCGAAGTCATTCTCGATGCGCTACGCATGCGCTCGCTGATCGATGGTCTGCATTCCCGCTACAGCC
GCTCGATCGTGGAACAGGCGGCCATTGCCGGCGCGCTGAACCCGGAGCTTTCTGCCGATGCGGCCCGTGCTCAGGAAACC
GTTGCCGAAGTGGCCCGGCGTCTGGACATGATTGCGGAAGAAACCGAGCGCGGCTGGTCGGGCGTAGTTCTGGAGGATGG
CGGCCTGCGCTTCGAGCGCATGGTACGCGGCGTCAAGGAAATCTCGACGCTGGATATGGGCCTGCTCGGTTCAGCCGATG
CGCGCCATATCGATCAGCTGGTAGCCCGCACCCGCGATATCTATGCAACGCCGCCGGTTCTGCAGCGCAAGGATGGCAGC
ATGGAGCTGGCCGGACCACGCGCGCTTCTGGATGCGATTTTCGCCGCCGGCCGCAAGGGCCTTTCCATGCAGCGTTATAA
GGGCCTCGGCGAGATGAATGCGGAGCAGCTGTGGGAAACCACGCTCGACGCCAATGTCCGCTCCCTGCTGCAGGTGAGAG
TGAACGACGCGACCGATGCCGATGGCCTGTTCGCCCGCCTGATGGGCGATGAAGTGGAGCCGCGCCGCGACTTCATTCAG
GAAAATGCGTTGAATGTCGCCAACCTCGACATTTAA

Upstream 100 bases:

>100_bases
TGTAGAATTTCTATAGTCGCATGTCGGATTTGAGTGATTCGCTGGCTTCAGGCCTTCTCACTGAAACGCCTGGCCCGAGA
AAACTTTGGAAAGAGTACTT

Downstream 100 bases:

>100_bases
TCTTGTGGGGTGTTTCGCGTAACGATTCACGTGAAACGAAAATTTAACAAAAGGCCCGGCGGTGAATTTCCGCCGGGCCT
TTTGTTTGATGGTGTCGGGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MTETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNADGSVT
VTDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVA
DAPLSVIGEYEGRSGTEVTFLASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRY
LDRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAALTRQVTSYADTSGIMKREKV
SLQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPVVESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKA
RELTRRKGALDIASLPGKLADCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIG
TLITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSVQYLKD
EKALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALRMRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQET
VAEVARRLDMIAEETERGWSGVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGS
MELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDADGLFARLMGDEVEPRRDFIQ
ENALNVANLDI

Sequences:

>Translated_811_residues
MTETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNADGSVT
VTDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVA
DAPLSVIGEYEGRSGTEVTFLASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRY
LDRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAALTRQVTSYADTSGIMKREKV
SLQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPVVESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKA
RELTRRKGALDIASLPGKLADCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIG
TLITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSVQYLKD
EKALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALRMRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQET
VAEVARRLDMIAEETERGWSGVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGS
MELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDADGLFARLMGDEVEPRRDFIQ
ENALNVANLDI
>Mature_810_residues
TETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNADGSVTV
TDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVAD
APLSVIGEYEGRSGTEVTFLASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRYL
DRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAALTRQVTSYADTSGIMKREKVS
LQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPVVESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKAR
ELTRRKGALDIASLPGKLADCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIGT
LITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGKSVQYLKDE
KALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALRMRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQETV
AEVARRLDMIAEETERGWSGVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGSM
ELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDADGLFARLMGDEVEPRRDFIQE
NALNVANLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=563, Percent_Identity=24.6891651865009, Blast_Score=134, Evalue=5e-31,
Organism=Homo sapiens, GI19913408, Length=551, Percent_Identity=24.6823956442831, Blast_Score=130, Evalue=7e-30,
Organism=Escherichia coli, GI48994957, Length=806, Percent_Identity=58.4367245657568, Blast_Score=891, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=589, Percent_Identity=40.7470288624788, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=24.4897959183673, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=543, Percent_Identity=23.2044198895028, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=141, Percent_Identity=34.7517730496454, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=589, Percent_Identity=26.8251273344652, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17136538, Length=532, Percent_Identity=25.3759398496241, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88859; Mature: 88728

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
ALAGHADLVTVTLNADGSVTVTDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYK
HHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVADAPLSVIGEYEGRSGTEVTF
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCEEEE
LASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRY
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHH
LDRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAA
HHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHH
LTRQVTSYADTSGIMKREKVSLQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHH
VESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKARELTRRKGALDIASLPGKLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
DCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIG
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHH
TLITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLY
HHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
IAQPPLYKVTRGKSVQYLKDEKALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALR
EECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCEEECCHHHHHHHHHHH
MRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQETVAEVARRLDMIAEETERGWS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGS
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
MELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDA
CEECCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
DGLFARLMGDEVEPRRDFIQENALNVANLDI
HHHHHHHHCCCCCHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
TETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
ALAGHADLVTVTLNADGSVTVTDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYK
HHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVADAPLSVIGEYEGRSGTEVTF
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCEEEE
LASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRY
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHH
LDRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAA
HHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHH
LTRQVTSYADTSGIMKREKVSLQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHH
VESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKARELTRRKGALDIASLPGKLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
DCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIG
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHH
TLITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLY
HHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
IAQPPLYKVTRGKSVQYLKDEKALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALR
EECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCEEECCHHHHHHHHHHH
MRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQETVAEVARRLDMIAEETERGWS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGS
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
MELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDA
CEECCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
DGLFARLMGDEVEPRRDFIQENALNVANLDI
HHHHHHHHCCCCCHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]