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The map label for this gene is trmE

Identifier: 15890077

GI number: 15890077

Start: 2836929

End: 2838257

Strand: Reverse

Name: trmE

Synonym: Atu2832

Alternate gene names: 15890077

Gene position: 2838257-2836929 (Counterclockwise)

Preceding gene: 15890078

Following gene: 15890076

Centisome position: 99.88

GC content: 55.0

Gene sequence:

>1329_bases
ATGCCAGATTCCGCCGATACGATTTATGCGCTGTCCAGCGGTGCACTGCCTGCTGGTGTCGCGGTTATCAGAATCAGCGG
CGCAAAGGCTTTTATTGCCTTAAGGGCGCTGACAGGCCGGGACCTTCCTTTACCAAGAACAGCAAGCCTCTGTTCGATTC
GGAATCGAAACAATGAGATCATCGACCAGAGCCTGGTGATCGTCTTCCCGGCTCCGAATTCCTTTACCGGCGAAAATTGC
GTTGAAATTCACAGCCACGGTAGTCGCGCAGTCATGGCTTCCATCTTCGCCGAGCTTGACAATCTCGGAGGACTGCGGCC
TGCCGACGCCGGTGAGTTTTCCCGCAGAGCCTTTGAGAATGGAAAGATGGACCTGCTTGAGGTCGAGGGTCTGGCCGATC
TCTTGCAGGCGGAAACGGAAATGCAGCGCCGTCTGGCGGTTGAGCAGAGCAGCGGCCAACTATCGGCTCTCTATGATGGC
TGGGCCAATCGCCTGACCCGTGCCCGCGCGCTGATCGAGGCGGAACTGGATTTCGCCGACGAGGAAGACGTGCCTGACTC
CGTTGCTACTCAGGTTTGGGAGGCAATGGCGGCGCTAAAGGGCGAGATCAATGCTCACCTCCAGGGCGGCGGGAATGGCG
AAATCATCCGCGACGGTTTCAAGGTTGCGCTTGTAGGCGAACCGAACGCGGGTAAATCCACCTTGCTTAACGCATTGAGC
GGTCGTGAGGTGGCAATCGTTACCGACATTGCCGGCACGACGCGGGACGTTTTAAGCGTCGATATCAATCTGGACGGATA
TCTGGTTCGGATATTCGATACGGCGGGAATTCGCGAGACACAGGATGTGGTGGAACGAGAAGGCGTGCGGCGTGCGGTTC
TGACCGCGGAAACTGCGGATCTCATTCTAATTCTGCAGGATAACGATTCGACCCCGAAACAATCTATCGGCTCGTTCGAT
AATCAGAGATCCTTGCGGGTAAGGACAAAAACCTTGCTTCGCTCACGCGCATCTGACGATGATTTTGATCTCTCGATCTC
AGCAAAAGAAGGAATTGGCCTAGATGAACTGCGTAGAGCCCTCAAGCGAGAGATAGAAAAACGGGTCGGTTCTGGCCAGA
CACTGGTTCCCGCCCGTGCCCGTCATAAAAAACGACTTGAAGAGACGCTGAACTATGTTAGTGATGCCCTCGATTCTGAG
ACCCTGGACCTTGCTATAAGGTCGGAATATCTAAGGCTCGCCGCAACATCGCTTGGACGGATCACCGGCCGCGTCGATGT
GGAAGATTTGCTGGGTGTGATCTTCTCGGAATTCTGCATAGGAAAATGA

Upstream 100 bases:

>100_bases
TGAATACCTGATCCGAACTTTAGTCGGATTTATCATAAGAGCTTTAAAGCCGTATCCGTTCATTCGGGTGCGGCTTGACT
GTTTTAGACCGAGGTGAACG

Downstream 100 bases:

>100_bases
TTCACGTGAAACATCGGCACTCTGTATGGTGATGTCGATGAATTAGAACAGGGCGCAGCAATATGCACCAGTCATTCGAT
GTCGTTGTTATTGGTGGTGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPDSADTIYALSSGALPAGVAVIRISGAKAFIALRALTGRDLPLPRTASLCSIRNRNNEIIDQSLVIVFPAPNSFTGENC
VEIHSHGSRAVMASIFAELDNLGGLRPADAGEFSRRAFENGKMDLLEVEGLADLLQAETEMQRRLAVEQSSGQLSALYDG
WANRLTRARALIEAELDFADEEDVPDSVATQVWEAMAALKGEINAHLQGGGNGEIIRDGFKVALVGEPNAGKSTLLNALS
GREVAIVTDIAGTTRDVLSVDINLDGYLVRIFDTAGIRETQDVVEREGVRRAVLTAETADLILILQDNDSTPKQSIGSFD
NQRSLRVRTKTLLRSRASDDDFDLSISAKEGIGLDELRRALKREIEKRVGSGQTLVPARARHKKRLEETLNYVSDALDSE
TLDLAIRSEYLRLAATSLGRITGRVDVEDLLGVIFSEFCIGK

Sequences:

>Translated_442_residues
MPDSADTIYALSSGALPAGVAVIRISGAKAFIALRALTGRDLPLPRTASLCSIRNRNNEIIDQSLVIVFPAPNSFTGENC
VEIHSHGSRAVMASIFAELDNLGGLRPADAGEFSRRAFENGKMDLLEVEGLADLLQAETEMQRRLAVEQSSGQLSALYDG
WANRLTRARALIEAELDFADEEDVPDSVATQVWEAMAALKGEINAHLQGGGNGEIIRDGFKVALVGEPNAGKSTLLNALS
GREVAIVTDIAGTTRDVLSVDINLDGYLVRIFDTAGIRETQDVVEREGVRRAVLTAETADLILILQDNDSTPKQSIGSFD
NQRSLRVRTKTLLRSRASDDDFDLSISAKEGIGLDELRRALKREIEKRVGSGQTLVPARARHKKRLEETLNYVSDALDSE
TLDLAIRSEYLRLAATSLGRITGRVDVEDLLGVIFSEFCIGK
>Mature_441_residues
PDSADTIYALSSGALPAGVAVIRISGAKAFIALRALTGRDLPLPRTASLCSIRNRNNEIIDQSLVIVFPAPNSFTGENCV
EIHSHGSRAVMASIFAELDNLGGLRPADAGEFSRRAFENGKMDLLEVEGLADLLQAETEMQRRLAVEQSSGQLSALYDGW
ANRLTRARALIEAELDFADEEDVPDSVATQVWEAMAALKGEINAHLQGGGNGEIIRDGFKVALVGEPNAGKSTLLNALSG
REVAIVTDIAGTTRDVLSVDINLDGYLVRIFDTAGIRETQDVVEREGVRRAVLTAETADLILILQDNDSTPKQSIGSFDN
QRSLRVRTKTLLRSRASDDDFDLSISAKEGIGLDELRRALKREIEKRVGSGQTLVPARARHKKRLEETLNYVSDALDSET
LDLAIRSEYLRLAATSLGRITGRVDVEDLLGVIFSEFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=44.0860215053763, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI193082993, Length=452, Percent_Identity=44.0265486725664, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=41.4486921529175, Blast_Score=313, Evalue=3e-85,
Organism=Escherichia coli, GI2367268, Length=459, Percent_Identity=37.9084967320261, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87082120, Length=90, Percent_Identity=38.8888888888889, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=31.9290465631929, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=34.2105263157895, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=39.323467230444, Blast_Score=299, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_AGRT5 (A9CHB2)

Other databases:

- EMBL:   AE007869
- PIR:   AG2924
- PIR:   F97698
- RefSeq:   NP_355758.1
- ProteinModelPortal:   A9CHB2
- SMR:   A9CHB2
- STRING:   A9CHB2
- GeneID:   1134870
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2832
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   A9CHB2
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47959; Mature: 47828

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 82-82 BINDING 122-122 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSADTIYALSSGALPAGVAVIRISGAKAFIALRALTGRDLPLPRTASLCSIRNRNNEI
CCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
IDQSLVIVFPAPNSFTGENCVEIHSHGSRAVMASIFAELDNLGGLRPADAGEFSRRAFEN
ECCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
GKMDLLEVEGLADLLQAETEMQRRLAVEQSSGQLSALYDGWANRLTRARALIEAELDFAD
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEDVPDSVATQVWEAMAALKGEINAHLQGGGNGEIIRDGFKVALVGEPNAGKSTLLNALS
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEEEEEEECCCCCHHHHHHHHC
GREVAIVTDIAGTTRDVLSVDINLDGYLVRIFDTAGIRETQDVVEREGVRRAVLTAETAD
CCEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCHHHHHEEECCCC
LILILQDNDSTPKQSIGSFDNQRSLRVRTKTLLRSRASDDDFDLSISAKEGIGLDELRRA
EEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHH
LKREIEKRVGSGQTLVPARARHKKRLEETLNYVSDALDSETLDLAIRSEYLRLAATSLGR
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ITGRVDVEDLLGVIFSEFCIGK
HCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDSADTIYALSSGALPAGVAVIRISGAKAFIALRALTGRDLPLPRTASLCSIRNRNNEI
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
IDQSLVIVFPAPNSFTGENCVEIHSHGSRAVMASIFAELDNLGGLRPADAGEFSRRAFEN
ECCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
GKMDLLEVEGLADLLQAETEMQRRLAVEQSSGQLSALYDGWANRLTRARALIEAELDFAD
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEDVPDSVATQVWEAMAALKGEINAHLQGGGNGEIIRDGFKVALVGEPNAGKSTLLNALS
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEEEEEEECCCCCHHHHHHHHC
GREVAIVTDIAGTTRDVLSVDINLDGYLVRIFDTAGIRETQDVVEREGVRRAVLTAETAD
CCEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCHHHHHEEECCCC
LILILQDNDSTPKQSIGSFDNQRSLRVRTKTLLRSRASDDDFDLSISAKEGIGLDELRRA
EEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHH
LKREIEKRVGSGQTLVPARARHKKRLEETLNYVSDALDSETLDLAIRSEYLRLAATSLGR
HHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ITGRVDVEDLLGVIFSEFCIGK
HCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194