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The map label for this gene is gidA

Identifier: 15890076

GI number: 15890076

Start: 2834983

End: 2836866

Strand: Reverse

Name: gidA

Synonym: Atu2831

Alternate gene names: 15890076

Gene position: 2836866-2834983 (Counterclockwise)

Preceding gene: 15890077

Following gene: 15890075

Centisome position: 99.83

GC content: 54.41

Gene sequence:

>1884_bases
ATGCACCAGTCATTCGATGTCGTTGTTATTGGTGGTGGTCATGCGGGCAGCGAAGCTGCGGCGGCCGCTGCGAGACATGG
TGCAAAGACCGCTCTTGTTACCCACAAGCGTGAGGCGATCGGGGTCATGTCCTGTAATCCAGCGATCGGTGGCTTGGGAA
AGGGGCACCTCGTTCGTGAGATCGATGCGCTGGACGGATTGATGGGCAGGGTCGCAGATGCTGCGGGAATTCAATTCCGT
ATGCTCAACCGGAAAAAGGGCCCAGCGGTTCGTGGGCCGCGCACGCAAGCTGACCGCCGGCTCTATCGTGAAGCGATGCA
GCGGGAAATCGATGCGATGGACAACCTGACAATTATCGAAGGCGATGCTTTTGATATTGAAATGGACAACGATCGGGTCG
CTGCGGTTATCATGAAAAACGGTAGCCGGATTCCTTGCGGCGCAGTGGTACTAACCAGCGGGACTTTTCTGCGCGGTCTT
ATCCATATCGGTTCCGAAAAGATTCCAGCCGGCCGCGTTGGCGAAATGCCCTCTCTCGGCCTTTCTGACACGCTATCGCG
CCTTGGTCTCGCAATGGGTCGGCTCAAAACCGGCACCCCTGCCCGTTTGGATGGACGTACAATCGATTGGAATGCGGTAG
ATCGGCAGGCAGCTGATGAGGATCCGGTCCCGTTCTCATTTATGACGGACCATATTCTTAATCCGCAAATCGAATGTGGT
GTGACCCGCACGACCCCGGCAAGTCACAAGATCATCCAGGATAATATTCACCTCTCTGCCATGTATTCCGGGCAGATCGA
AGGCGTTGGCCCGCGTTATTGCCCGTCAATCGAGGATAAGATCACCCGTTTCGGGGAACGGGACGGCCACCAGATATTTT
TGGAGCCGGAAGGCCTCGATGATTATACCATTTATCCCAATGGTATTTCGACATCCCTGCCCGCTTTTGTTCAGGAGCAA
TTTATCCGGACGATACCAGGCCTTGAACAGGTTACGATTCTGCAGCCGGGTTATGCGATCGAATATGATTATGTCGATCC
CCGGGAGCTGAAACCGTCTCTTGAGTGCCGCAAGATTCCGGGGCTGTTTCTGGCTGGCCAGATCAACGGCACGACTGGTT
ATGAAGAGGCGGGCGCCCAGGGTCTTGTTGCTGGTCTGAATGCCTCGCTTTATGCCGGCAGTGCCGATCCGCTTCATTTT
AGCCGGACAGAATCTTATATCGGCGTGATGATCGACGACTTGACATCCAAGGGCGTCAGCGAACCATATCGTATGTTTAC
GTCAAGGGCGGAGTATCGTCTTTCCCTGCGCGTGGATAATGCCGATCTCCGACTGACACCCGTCGCCCAGAAGGCAGGTA
TTCTAGGACGAGAGCGCCAGCAGCGCTTTACAGACTTCTTGTCCGATCTTGATAGCGTTCGTGCGTTGATGAAAGAGCTG
TCCATCAGCCCGTCCCAAGCGGCCAAGCAGGGTCTGAAACTCAATCAGGATGGTCAGCGTCGTTCCGTTTATGAGCTGCT
TGCCTATCCTGATATGACATTGCAGGCGCTCGCAGAACATTGGCCTGAACTCAACAGGCTTAACGCTAAGGTCGCGGAGG
TCTTGCAGATTGAAGCGAGCTACGCCGTCTATATGCAACGGCAGAGCGCTGATATTGTCGATATCAAAAGGGATGAGGAC
AGAAAAATCCCTGACGATTTCGATTTTCAAAGTCTGTCCGGGCTCTCCAACGAGCTTAAACAAAAGCTGGAAAAAGCCCG
CCCGGAGAATATTGCACAGGCCGCTCGTGTTGACGGCATGACGCCAGCTGCGATATCGCTGCTGCTCGCACTTCTGCGGA
AAGGTACCGCAACGCGAAGTGAACAATTGCGCATGCACCAATAG

Upstream 100 bases:

>100_bases
TGGGTGTGATCTTCTCGGAATTCTGCATAGGAAAATGATTCACGTGAAACATCGGCACTCTGTATGGTGATGTCGATGAA
TTAGAACAGGGCGCAGCAAT

Downstream 100 bases:

>100_bases
CGAGTCGGATTATGAAGATAAACGGCCAGAGTGTTTCACGTGAAACACAGGAGAGGCTCGAGCATTTTGTCGAGCTTTTC
AAGAAGTGGAATAAGACCAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MHQSFDVVVIGGGHAGSEAAAAAARHGAKTALVTHKREAIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
MLNRKKGPAVRGPRTQADRRLYREAMQREIDAMDNLTIIEGDAFDIEMDNDRVAAVIMKNGSRIPCGAVVLTSGTFLRGL
IHIGSEKIPAGRVGEMPSLGLSDTLSRLGLAMGRLKTGTPARLDGRTIDWNAVDRQAADEDPVPFSFMTDHILNPQIECG
VTRTTPASHKIIQDNIHLSAMYSGQIEGVGPRYCPSIEDKITRFGERDGHQIFLEPEGLDDYTIYPNGISTSLPAFVQEQ
FIRTIPGLEQVTILQPGYAIEYDYVDPRELKPSLECRKIPGLFLAGQINGTTGYEEAGAQGLVAGLNASLYAGSADPLHF
SRTESYIGVMIDDLTSKGVSEPYRMFTSRAEYRLSLRVDNADLRLTPVAQKAGILGRERQQRFTDFLSDLDSVRALMKEL
SISPSQAAKQGLKLNQDGQRRSVYELLAYPDMTLQALAEHWPELNRLNAKVAEVLQIEASYAVYMQRQSADIVDIKRDED
RKIPDDFDFQSLSGLSNELKQKLEKARPENIAQAARVDGMTPAAISLLLALLRKGTATRSEQLRMHQ

Sequences:

>Translated_627_residues
MHQSFDVVVIGGGHAGSEAAAAAARHGAKTALVTHKREAIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
MLNRKKGPAVRGPRTQADRRLYREAMQREIDAMDNLTIIEGDAFDIEMDNDRVAAVIMKNGSRIPCGAVVLTSGTFLRGL
IHIGSEKIPAGRVGEMPSLGLSDTLSRLGLAMGRLKTGTPARLDGRTIDWNAVDRQAADEDPVPFSFMTDHILNPQIECG
VTRTTPASHKIIQDNIHLSAMYSGQIEGVGPRYCPSIEDKITRFGERDGHQIFLEPEGLDDYTIYPNGISTSLPAFVQEQ
FIRTIPGLEQVTILQPGYAIEYDYVDPRELKPSLECRKIPGLFLAGQINGTTGYEEAGAQGLVAGLNASLYAGSADPLHF
SRTESYIGVMIDDLTSKGVSEPYRMFTSRAEYRLSLRVDNADLRLTPVAQKAGILGRERQQRFTDFLSDLDSVRALMKEL
SISPSQAAKQGLKLNQDGQRRSVYELLAYPDMTLQALAEHWPELNRLNAKVAEVLQIEASYAVYMQRQSADIVDIKRDED
RKIPDDFDFQSLSGLSNELKQKLEKARPENIAQAARVDGMTPAAISLLLALLRKGTATRSEQLRMHQ
>Mature_627_residues
MHQSFDVVVIGGGHAGSEAAAAAARHGAKTALVTHKREAIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
MLNRKKGPAVRGPRTQADRRLYREAMQREIDAMDNLTIIEGDAFDIEMDNDRVAAVIMKNGSRIPCGAVVLTSGTFLRGL
IHIGSEKIPAGRVGEMPSLGLSDTLSRLGLAMGRLKTGTPARLDGRTIDWNAVDRQAADEDPVPFSFMTDHILNPQIECG
VTRTTPASHKIIQDNIHLSAMYSGQIEGVGPRYCPSIEDKITRFGERDGHQIFLEPEGLDDYTIYPNGISTSLPAFVQEQ
FIRTIPGLEQVTILQPGYAIEYDYVDPRELKPSLECRKIPGLFLAGQINGTTGYEEAGAQGLVAGLNASLYAGSADPLHF
SRTESYIGVMIDDLTSKGVSEPYRMFTSRAEYRLSLRVDNADLRLTPVAQKAGILGRERQQRFTDFLSDLDSVRALMKEL
SISPSQAAKQGLKLNQDGQRRSVYELLAYPDMTLQALAEHWPELNRLNAKVAEVLQIEASYAVYMQRQSADIVDIKRDED
RKIPDDFDFQSLSGLSNELKQKLEKARPENIAQAARVDGMTPAAISLLLALLRKGTATRSEQLRMHQ

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=643, Percent_Identity=45.7231726283048, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI19882217, Length=668, Percent_Identity=44.0119760479042, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI183227703, Length=683, Percent_Identity=43.0453879941435, Blast_Score=518, Evalue=1e-147,
Organism=Escherichia coli, GI2367273, Length=616, Percent_Identity=51.1363636363636, Blast_Score=598, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=45.016077170418, Blast_Score=525, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6321202, Length=628, Percent_Identity=47.6114649681529, Blast_Score=557, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24658174, Length=634, Percent_Identity=47.6340694006309, Blast_Score=548, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_AGRT5 (Q8UBM0)

Other databases:

- EMBL:   AE007869
- PIR:   AF2924
- PIR:   E97698
- RefSeq:   NP_355757.1
- ProteinModelPortal:   Q8UBM0
- SMR:   Q8UBM0
- STRING:   Q8UBM0
- GeneID:   1134869
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2831
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q8UBM0
- ProtClustDB:   PRK05192
- BioCyc:   ATUM176299-1:ATU2831-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68767; Mature: 68767

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQSFDVVVIGGGHAGSEAAAAAARHGAKTALVTHKREAIGVMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHEEEEECCCCCCCCCCCHHHHH
IDALDGLMGRVADAAGIQFRMLNRKKGPAVRGPRTQADRRLYREAMQREIDAMDNLTIIE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
GDAFDIEMDNDRVAAVIMKNGSRIPCGAVVLTSGTFLRGLIHIGSEKIPAGRVGEMPSLG
CCEEEEEECCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
LSDTLSRLGLAMGRLKTGTPARLDGRTIDWNAVDRQAADEDPVPFSFMTDHILNPQIECG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEECC
VTRTTPASHKIIQDNIHLSAMYSGQIEGVGPRYCPSIEDKITRFGERDGHQIFLEPEGLD
CCCCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCC
DYTIYPNGISTSLPAFVQEQFIRTIPGLEQVTILQPGYAIEYDYVDPRELKPSLECRKIP
CEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCCCCHHCCC
GLFLAGQINGTTGYEEAGAQGLVAGLNASLYAGSADPLHFSRTESYIGVMIDDLTSKGVS
CEEEEEEECCCCCCHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCHHHEEHHHHCCCCC
EPYRMFTSRAEYRLSLRVDNADLRLTPVAQKAGILGRERQQRFTDFLSDLDSVRALMKEL
HHHHHHHCCCCEEEEEEECCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SISPSQAAKQGLKLNQDGQRRSVYELLAYPDMTLQALAEHWPELNRLNAKVAEVLQIEAS
CCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCH
YAVYMQRQSADIVDIKRDEDRKIPDDFDFQSLSGLSNELKQKLEKARPENIAQAARVDGM
HEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TPAAISLLLALLRKGTATRSEQLRMHQ
CHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MHQSFDVVVIGGGHAGSEAAAAAARHGAKTALVTHKREAIGVMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHEEEEECCCCCCCCCCCHHHHH
IDALDGLMGRVADAAGIQFRMLNRKKGPAVRGPRTQADRRLYREAMQREIDAMDNLTIIE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
GDAFDIEMDNDRVAAVIMKNGSRIPCGAVVLTSGTFLRGLIHIGSEKIPAGRVGEMPSLG
CCEEEEEECCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
LSDTLSRLGLAMGRLKTGTPARLDGRTIDWNAVDRQAADEDPVPFSFMTDHILNPQIECG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEECC
VTRTTPASHKIIQDNIHLSAMYSGQIEGVGPRYCPSIEDKITRFGERDGHQIFLEPEGLD
CCCCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCC
DYTIYPNGISTSLPAFVQEQFIRTIPGLEQVTILQPGYAIEYDYVDPRELKPSLECRKIP
CEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCCCCHHCCC
GLFLAGQINGTTGYEEAGAQGLVAGLNASLYAGSADPLHFSRTESYIGVMIDDLTSKGVS
CEEEEEEECCCCCCHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCHHHEEHHHHCCCCC
EPYRMFTSRAEYRLSLRVDNADLRLTPVAQKAGILGRERQQRFTDFLSDLDSVRALMKEL
HHHHHHHCCCCEEEEEEECCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SISPSQAAKQGLKLNQDGQRRSVYELLAYPDMTLQALAEHWPELNRLNAKVAEVLQIEAS
CCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCH
YAVYMQRQSADIVDIKRDEDRKIPDDFDFQSLSGLSNELKQKLEKARPENIAQAARVDGM
HEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TPAAISLLLALLRKGTATRSEQLRMHQ
CHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194