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The map label for this gene is gidB

Identifier: 15890075

GI number: 15890075

Start: 2834354

End: 2834971

Strand: Reverse

Name: gidB

Synonym: Atu2830

Alternate gene names: 15890075

Gene position: 2834971-2834354 (Counterclockwise)

Preceding gene: 15890076

Following gene: 15890074

Centisome position: 99.77

GC content: 48.06

Gene sequence:

>618_bases
ATGAAGATAAACGGCCAGAGTGTTTCACGTGAAACACAGGAGAGGCTCGAGCATTTTGTCGAGCTTTTCAAGAAGTGGAA
TAAGACCACGAATCTGGTAGCGCCGTCCACGCTAACGGAGCTTTGGAGCCGGCATGTTGCCGATAGCGCGCAAATCTTCC
AGCTTTCTCCTCAACCAAAAAAATGGATTGATCTCGGCAGCGGCGGCGGTTTTCCCGGTGTCATCACGGCAATTTTTCTG
GCGGAACTTGGCGATGGCTGGGTCGATCTGGTGGAAAGCAATAATAAGAAAGCCGCTTTTCTGCGAATCGCTCTTAAGGA
AACCGGTGCCAGAGGCGCGGTTCATCCTGTCAGAATCGAGACGGCGTCTGAAATTATAACAACTTGTAATGCTGTGTCCG
CTCGTGCCCTTGCAGATCTGGATTTACTTTTTGGGTATATTCATCCCTGGGCGCAAAATAATCCTAATCTCAAGGCTTAT
TTTAATAAAGGCAGGGATTATGATTCTGAAGTGCAGAAAGCGCATGGTCGCTGGCAGTTTGATCTGATAAAACATCGGAG
TGCCATTGAGGCAGATTCTGTTGTTCTTGAAATCAGCAACCTCGTTTTGAGACGCTGA

Upstream 100 bases:

>100_bases
GCATGACGCCAGCTGCGATATCGCTGCTGCTCGCACTTCTGCGGAAAGGTACCGCAACGCGAAGTGAACAATTGCGCATG
CACCAATAGCGAGTCGGATT

Downstream 100 bases:

>100_bases
CAAGCTGGCCAGGTGCGGCATGACCTTTGAAAAAAACCGAATTATCGCAGTTGCGAACCAGAAAGGCGGCGTTGGCAAGA
CAACAACCGCTATCAATCTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKINGQSVSRETQERLEHFVELFKKWNKTTNLVAPSTLTELWSRHVADSAQIFQLSPQPKKWIDLGSGGGFPGVITAIFL
AELGDGWVDLVESNNKKAAFLRIALKETGARGAVHPVRIETASEIITTCNAVSARALADLDLLFGYIHPWAQNNPNLKAY
FNKGRDYDSEVQKAHGRWQFDLIKHRSAIEADSVVLEISNLVLRR

Sequences:

>Translated_205_residues
MKINGQSVSRETQERLEHFVELFKKWNKTTNLVAPSTLTELWSRHVADSAQIFQLSPQPKKWIDLGSGGGFPGVITAIFL
AELGDGWVDLVESNNKKAAFLRIALKETGARGAVHPVRIETASEIITTCNAVSARALADLDLLFGYIHPWAQNNPNLKAY
FNKGRDYDSEVQKAHGRWQFDLIKHRSAIEADSVVLEISNLVLRR
>Mature_205_residues
MKINGQSVSRETQERLEHFVELFKKWNKTTNLVAPSTLTELWSRHVADSAQIFQLSPQPKKWIDLGSGGGFPGVITAIFL
AELGDGWVDLVESNNKKAAFLRIALKETGARGAVHPVRIETASEIITTCNAVSARALADLDLLFGYIHPWAQNNPNLKAY
FNKGRDYDSEVQKAHGRWQFDLIKHRSAIEADSVVLEISNLVLRR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_AGRT5 (Q8UBM1)

Other databases:

- EMBL:   AE007869
- PIR:   AE2924
- PIR:   D97698
- RefSeq:   NP_355756.1
- ProteinModelPortal:   Q8UBM1
- SMR:   Q8UBM1
- STRING:   Q8UBM1
- GeneID:   1134868
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2830
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   ARGRWEF
- ProtClustDB:   PRK00107
- BioCyc:   ATUM176299-1:ATU2830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22965; Mature: 22965

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINGQSVSRETQERLEHFVELFKKWNKTTNLVAPSTLTELWSRHVADSAQIFQLSPQPK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCH
KWIDLGSGGGFPGVITAIFLAELGDGWVDLVESNNKKAAFLRIALKETGARGAVHPVRIE
HHEECCCCCCCHHHHHHHHHHHHCCCCEEHHHCCCCCEEEEEEEEHHCCCCCCCCEEEEH
TASEIITTCNAVSARALADLDLLFGYIHPWAQNNPNLKAYFNKGRDYDSEVQKAHGRWQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEH
DLIKHRSAIEADSVVLEISNLVLRR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKINGQSVSRETQERLEHFVELFKKWNKTTNLVAPSTLTELWSRHVADSAQIFQLSPQPK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCH
KWIDLGSGGGFPGVITAIFLAELGDGWVDLVESNNKKAAFLRIALKETGARGAVHPVRIE
HHEECCCCCCCHHHHHHHHHHHHCCCCEEHHHCCCCCEEEEEEEEHHCCCCCCCCEEEEH
TASEIITTCNAVSARALADLDLLFGYIHPWAQNNPNLKAYFNKGRDYDSEVQKAHGRWQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEH
DLIKHRSAIEADSVVLEISNLVLRR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194