Click here to switch to the map view.

The map label for this gene is cobJ [H]

Identifier: 15890047

GI number: 15890047

Start: 2801470

End: 2802231

Strand: Reverse

Name: cobJ [H]

Synonym: Atu2800

Alternate gene names: 15890047

Gene position: 2802231-2801470 (Counterclockwise)

Preceding gene: 15890048

Following gene: 159185387

Centisome position: 98.62

GC content: 60.24

Gene sequence:

>762_bases
ATGACCGGCAAACTCGTTGTTATCGGCCTTGGTCCCGGCAACGGAAATCAGGTGACGCCTGAGGCGCTGGCCGCTGTCGA
GTCATCGGAAGATTTTTTCGGTTACATCCCCTATCTCGACCGCCTTTCGCTTGGGCCTCACCAGCGGCGTCATGCTTCCG
ACAATCGTGAGGAAATCTCCCGCGCCGAACAGGCGCTCACGCTTGCGGCCGAAGGCGGCAACGTCTGCGTGGTGTCAGGC
GGTGATCCCGGTGTTTTCGCCATGGCGGCGGCGATTTGCGAGGCGATTGAAAACGGCCCTCTCGAATGGCGTGACATCGA
CTTTTCCGTCGTGCCTGGTGTGACCGCCATGCTCGCCGTCGCTGCAAAGGCCGGTGCGCCACTCGGCCATGATTTCTGCG
CCATTTCGCTTTCAGATAACCTCAAGCCCTGGGGCATTATCGAAAAACGGCTGATTGCAGCGGCCGAAGCCGGATTCGTG
ATGGCTTTCTACAATCCTGTTAGCAAGGCGCGGCCGCACCAGCTTTCTACCGCCTTCGACCTGTTGCGCAAACATTTGCC
CGGCTCGGTACCGGTGATATTCGGCCGCGCCGCAGGCCGGGTTGATGAACGTATTCGGGTGGTGCCCCTGTCGGAGGCGT
CGAGCGACATGGCCGACATGGCCACCTGCATCATCGTCGGCTCGGTGGAAACCCGCCTGATCGCGCGTTCCGGCAAGGAA
CCGATCGTTTATTCGCCACGGTTTTCGAAAAGGGAAAGCTGA

Upstream 100 bases:

>100_bases
ACGCGGTGCGGCTCATCGAGCGTGATGCCTCGCCCGCGCCCTATTTTTCGCTGGTTCTGGTACCAGGCTGGAAAACCAGG
CCAGCAGGGAGCGAAGGGTC

Downstream 100 bases:

>100_bases
TGGCGTATGGCGGTGAGCGCAGTGGCGATATCCGGCACGGTCACATTATTCGCGGGCGTATCGTTCAATTGTTGCGGCCG
TTCGATCATGATGACCGGCA

Product: precorrin-3B C17-methyltransferase

Products: NA

Alternate protein names: Precorrin-3 methylase; Precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTGKLVVIGLGPGNGNQVTPEALAAVESSEDFFGYIPYLDRLSLGPHQRRHASDNREEISRAEQALTLAAEGGNVCVVSG
GDPGVFAMAAAICEAIENGPLEWRDIDFSVVPGVTAMLAVAAKAGAPLGHDFCAISLSDNLKPWGIIEKRLIAAAEAGFV
MAFYNPVSKARPHQLSTAFDLLRKHLPGSVPVIFGRAAGRVDERIRVVPLSEASSDMADMATCIIVGSVETRLIARSGKE
PIVYSPRFSKRES

Sequences:

>Translated_253_residues
MTGKLVVIGLGPGNGNQVTPEALAAVESSEDFFGYIPYLDRLSLGPHQRRHASDNREEISRAEQALTLAAEGGNVCVVSG
GDPGVFAMAAAICEAIENGPLEWRDIDFSVVPGVTAMLAVAAKAGAPLGHDFCAISLSDNLKPWGIIEKRLIAAAEAGFV
MAFYNPVSKARPHQLSTAFDLLRKHLPGSVPVIFGRAAGRVDERIRVVPLSEASSDMADMATCIIVGSVETRLIARSGKE
PIVYSPRFSKRES
>Mature_252_residues
TGKLVVIGLGPGNGNQVTPEALAAVESSEDFFGYIPYLDRLSLGPHQRRHASDNREEISRAEQALTLAAEGGNVCVVSGG
DPGVFAMAAAICEAIENGPLEWRDIDFSVVPGVTAMLAVAAKAGAPLGHDFCAISLSDNLKPWGIIEKRLIAAAEAGFVM
AFYNPVSKARPHQLSTAFDLLRKHLPGSVPVIFGRAAGRVDERIRVVPLSEASSDMADMATCIIVGSVETRLIARSGKEP
IVYSPRFSKRES

Specific function: Methyltransferase that catalyzes the methylation of C-17 in precorrin-3B to form precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=27.8688524590164, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.131 [H]

Molecular weight: Translated: 26884; Mature: 26753

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKLVVIGLGPGNGNQVTPEALAAVESSEDFFGYIPYLDRLSLGPHQRRHASDNREEIS
CCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEECCHHHHCCCCCCHHHCCCCCHHHHH
RAEQALTLAAEGGNVCVVSGGDPGVFAMAAAICEAIENGPLEWRDIDFSVVPGVTAMLAV
HHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCEEHHCHHHHHHHH
AAKAGAPLGHDFCAISLSDNLKPWGIIEKRLIAAAEAGFVMAFYNPVSKARPHQLSTAFD
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHHHHH
LLRKHLPGSVPVIFGRAAGRVDERIRVVPLSEASSDMADMATCIIVGSVETRLIARSGKE
HHHHHCCCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHEEEEEECCHHHHHHHHCCCC
PIVYSPRFSKRES
CEEECCCCCCCCC
>Mature Secondary Structure 
TGKLVVIGLGPGNGNQVTPEALAAVESSEDFFGYIPYLDRLSLGPHQRRHASDNREEIS
CCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEECCHHHHCCCCCCHHHCCCCCHHHHH
RAEQALTLAAEGGNVCVVSGGDPGVFAMAAAICEAIENGPLEWRDIDFSVVPGVTAMLAV
HHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCEEHHCHHHHHHHH
AAKAGAPLGHDFCAISLSDNLKPWGIIEKRLIAAAEAGFVMAFYNPVSKARPHQLSTAFD
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHHHHHH
LLRKHLPGSVPVIFGRAAGRVDERIRVVPLSEASSDMADMATCIIVGSVETRLIARSGKE
HHHHHCCCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHEEEEEECCHHHHHHHHCCCC
PIVYSPRFSKRES
CEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521; 8226690 [H]