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The map label for this gene is cobB

Identifier: 15890040

GI number: 15890040

Start: 2795035

End: 2796351

Strand: Reverse

Name: cobB

Synonym: Atu2793

Alternate gene names: 15890040

Gene position: 2796351-2795035 (Counterclockwise)

Preceding gene: 159185385

Following gene: 159185384

Centisome position: 98.41

GC content: 63.48

Gene sequence:

>1317_bases
ATGACGGCGCGGGCAATCATCATCGGTGCGCCGCGCTCGGGCTCCGGCAAGACCAGTGTGACCATCGGCCTGCTCCGGGC
TTTTGCGCGCCGCGGGGTGAAGGTGCGCGGCATCAAGACGGGGCCGGATTACATCGATCCCGGTTTTCATGCCTTCGCCA
CCGGCACACCCGGTCTCAATCTGGACAGCTGGGCCATGCAACCGGACCTGCTGCGACATCTGTTTTCGCAGCAGACCGAG
GATGCGGAACTCATTCTCATCGAAAGCGCCATGGGCCTGTTCGACGGTATTCCCGTGGCGGAAAACCGCACCGGCTCGGC
GGCGGATCTGGCGCGGCTGTTCCGCATTCCGGTTCTGCTGGTGCTGGATGTCTCCGGTCAGTCGCAGACGGCGGCTACCA
TTGCGCATGGTTTCGCCCACTACGATCCCGATGTCACCATGGGGGCGGTGGTGCTGAACCGGGCCGGCAGCGAAAGGCAC
CGGACGCTATGCACTGAAGCCATCGAAAAAATCGGCCTGCCCGTTGTCGGCTGCGTTCTGCGCGATCCTTCCCTCATCTT
GCCGGAGCGGCACCTGGGGCTGGTGCAAGCGAGTGAACATCCGGAGATTGACCCCCATATAGACCGGCTCGCGGATGCGA
TGGAAAAATCCATCGATCTCGACACCCTGTTTTCGCTCGCCGCGCCGGTGGATATGCCCTGCGGTTCGGTGGCGGCGGCG
ATTGCGCCGCCCGGTCAGCGCATCGCGCTGGCGGAAGATGCGGCTTTCACCTTCCTTTATCCGCATCTTAGGAGGCATTG
GCGTGCGGCCGGGGCGGAGATCGTGCCGTTTTCGCCGCTCGCCGATGAGGCGCCCGATGAGAGCTGCGATATTTGCTGGC
TGCCGGGCGGGTATCCCGAGCTTTTTGCCGGAAAACTCGCCGATGCTGTCGGCTTCAAGGCGGGCATTACCCGTTTTGCA
GAGACCAAGCCGGTGCATGGCGAGTGTGGCGGGTACATGGTGCTCGGTGAGAGGCTGGAAGATGCAGAGGGCGTGATCCA
TGCCATGACTGGTCTTCTGTCGCACGCCACGAGCTTTGCGACACGCAAGATGAATCTGGGCTACCGGCAGGCCACGATTG
CGGCAGACAGCCCGCTCGGCATGGCGGGGGACGTTCTGCGCGGCCATGAGTTTCATTATGCCCGGGTCATCGATCCCGGC
CGTGATCAGCCCTTCGCTCATATGGCTGACGGGCAGGGTCGTCCGCTCGGGCCATCGGGCGGCAGGAGAGGTTTTGTCTC
GGGCACGTTCTTCCACGCGATCGCCAAAGGCGGTTGA

Upstream 100 bases:

>100_bases
GCATAGCTGACGATGCAAAACGGGAGAGCTTCGAAGCGCCGGCTTTGATTATTATCGGCGAGATTGTTTCGATGCGGGAT
CGTCTTGGAGTGTCGGGCTC

Downstream 100 bases:

>100_bases
TCGTTTGCGGTTCCGGTCGGGGGACCTGCGGCAAAGTGGAGCAAACTGCGAAAGTTTCTAGGCTCCGATCACGGGAAAGT
GCTATAGGTCTCTTCATGGG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTARAIIIGAPRSGSGKTSVTIGLLRAFARRGVKVRGIKTGPDYIDPGFHAFATGTPGLNLDSWAMQPDLLRHLFSQQTE
DAELILIESAMGLFDGIPVAENRTGSAADLARLFRIPVLLVLDVSGQSQTAATIAHGFAHYDPDVTMGAVVLNRAGSERH
RTLCTEAIEKIGLPVVGCVLRDPSLILPERHLGLVQASEHPEIDPHIDRLADAMEKSIDLDTLFSLAAPVDMPCGSVAAA
IAPPGQRIALAEDAAFTFLYPHLRRHWRAAGAEIVPFSPLADEAPDESCDICWLPGGYPELFAGKLADAVGFKAGITRFA
ETKPVHGECGGYMVLGERLEDAEGVIHAMTGLLSHATSFATRKMNLGYRQATIAADSPLGMAGDVLRGHEFHYARVIDPG
RDQPFAHMADGQGRPLGPSGGRRGFVSGTFFHAIAKGG

Sequences:

>Translated_438_residues
MTARAIIIGAPRSGSGKTSVTIGLLRAFARRGVKVRGIKTGPDYIDPGFHAFATGTPGLNLDSWAMQPDLLRHLFSQQTE
DAELILIESAMGLFDGIPVAENRTGSAADLARLFRIPVLLVLDVSGQSQTAATIAHGFAHYDPDVTMGAVVLNRAGSERH
RTLCTEAIEKIGLPVVGCVLRDPSLILPERHLGLVQASEHPEIDPHIDRLADAMEKSIDLDTLFSLAAPVDMPCGSVAAA
IAPPGQRIALAEDAAFTFLYPHLRRHWRAAGAEIVPFSPLADEAPDESCDICWLPGGYPELFAGKLADAVGFKAGITRFA
ETKPVHGECGGYMVLGERLEDAEGVIHAMTGLLSHATSFATRKMNLGYRQATIAADSPLGMAGDVLRGHEFHYARVIDPG
RDQPFAHMADGQGRPLGPSGGRRGFVSGTFFHAIAKGG
>Mature_437_residues
TARAIIIGAPRSGSGKTSVTIGLLRAFARRGVKVRGIKTGPDYIDPGFHAFATGTPGLNLDSWAMQPDLLRHLFSQQTED
AELILIESAMGLFDGIPVAENRTGSAADLARLFRIPVLLVLDVSGQSQTAATIAHGFAHYDPDVTMGAVVLNRAGSERHR
TLCTEAIEKIGLPVVGCVLRDPSLILPERHLGLVQASEHPEIDPHIDRLADAMEKSIDLDTLFSLAAPVDMPCGSVAAAI
APPGQRIALAEDAAFTFLYPHLRRHWRAAGAEIVPFSPLADEAPDESCDICWLPGGYPELFAGKLADAVGFKAGITRFAE
TKPVHGECGGYMVLGERLEDAEGVIHAMTGLLSHATSFATRKMNLGYRQATIAADSPLGMAGDVLRGHEFHYARVIDPGR
DQPFAHMADGQGRPLGPSGGRRGFVSGTFFHAIAKGG

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_AGRT5 (Q8UBQ8)

Other databases:

- EMBL:   AE007869
- PIR:   A97694
- PIR:   AH2919
- RefSeq:   NP_355721.1
- ProteinModelPortal:   Q8UBQ8
- STRING:   Q8UBQ8
- GeneID:   1134831
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2793
- eggNOG:   COG1797
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   PRK01077
- BioCyc:   ATUM176299-1:ATU2793-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.9

Molecular weight: Translated: 46645; Mature: 46514

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 329-329 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAIIIGAPRSGSGKTSVTIGLLRAFARRGVKVRGIKTGPDYIDPGFHAFATGTPGLN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCC
LDSWAMQPDLLRHLFSQQTEDAELILIESAMGLFDGIPVAENRTGSAADLARLFRIPVLL
CCHHCCCHHHHHHHHHCCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEE
VLDVSGQSQTAATIAHGFAHYDPDVTMGAVVLNRAGSERHRTLCTEAIEKIGLPVVGCVL
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHEE
RDPSLILPERHLGLVQASEHPEIDPHIDRLADAMEKSIDLDTLFSLAAPVDMPCGSVAAA
CCCCEECCHHHCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
IAPPGQRIALAEDAAFTFLYPHLRRHWRAAGAEIVPFSPLADEAPDESCDICWLPGGYPE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEECCCCCHH
LFAGKLADAVGFKAGITRFAETKPVHGECGGYMVLGERLEDAEGVIHAMTGLLSHATSFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
TRKMNLGYRQATIAADSPLGMAGDVLRGHEFHYARVIDPGRDQPFAHMADGQGRPLGPSG
HHHHCCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCC
GRRGFVSGTFFHAIAKGG
CCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TARAIIIGAPRSGSGKTSVTIGLLRAFARRGVKVRGIKTGPDYIDPGFHAFATGTPGLN
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCC
LDSWAMQPDLLRHLFSQQTEDAELILIESAMGLFDGIPVAENRTGSAADLARLFRIPVLL
CCHHCCCHHHHHHHHHCCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEE
VLDVSGQSQTAATIAHGFAHYDPDVTMGAVVLNRAGSERHRTLCTEAIEKIGLPVVGCVL
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHEE
RDPSLILPERHLGLVQASEHPEIDPHIDRLADAMEKSIDLDTLFSLAAPVDMPCGSVAAA
CCCCEECCHHHCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
IAPPGQRIALAEDAAFTFLYPHLRRHWRAAGAEIVPFSPLADEAPDESCDICWLPGGYPE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEECCCCCHH
LFAGKLADAVGFKAGITRFAETKPVHGECGGYMVLGERLEDAEGVIHAMTGLLSHATSFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
TRKMNLGYRQATIAADSPLGMAGDVLRGHEFHYARVIDPGRDQPFAHMADGQGRPLGPSG
HHHHCCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCC
GRRGFVSGTFFHAIAKGG
CCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194