Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 15890038

GI number: 15890038

Start: 2792472

End: 2793584

Strand: Direct

Name: leuB

Synonym: Atu2791

Alternate gene names: 15890038

Gene position: 2792472-2793584 (Clockwise)

Preceding gene: 159185383

Following gene: 159185386

Centisome position: 98.27

GC content: 60.92

Gene sequence:

>1113_bases
ATGACAGTCCGTTCGCTTTTCCTGCTGCCCGGTGATGGTATCGGCCCTGAAGCCATGACTGAGGTCCGCAAGCTGATCGA
ATATATGAACAGCGCGCACAATGCCGGCTTTACCGTTTCTGAGGGCCTCGTCGGCGGTTCCGCTTACGACGCGCATGGCG
TCGCGATTTCCGATGCGGACATGGAAAAGGCGCTTGCCGCCGATGCAATCCTGTTCGGTGCCGTTGGCGGCCCGAAATGG
GATGGCGTGCCGTATGAGCATCGCCCGGAAGCCGGACTCCTGCGCCTGCGCAAGGATCTCGAACTTTTCGCCAATCTGCG
CCCGGCCATCTGCTATCCGGCGCTCGCTGCCGCCTCATCGCTAAAGCCGGAACTGGTCGAGGGTCTCGATATCCTCATCG
TTCGCGAACTGACCGGCGGCGTTTATTTCGGTGAACCGAAGCAGATCATCGATCTGGGCAACGGCCAGAAGCGCGGTATC
GACACGCAGATCTACGACACCTTCGAGATCGAGCGCATCGCCAGCGTCGCTTTCGAACTGGCCCGCAGCCGCGACAACCG
CGTTTGCTCGATGGAAAAGCGCAACGTGATGAAATCGGGCGTTTTGTGGAACCAGGTCGTCACCGAAACCCACGCGGCCA
AATACAAGGACGTGCAGCTAGAACATATGCTAGCCGATGCCGGCGGCATGCAGCTGGTGCGCAAGCCCAAGCAGTTCGAC
GTGATCGTCACCGACAACCTCTTCGGCGACATGCTGTCCGACGTGGCCGCCATGCTGACTGGCTCGCTCGGCATGCTGCC
ATCGGCCTCGCTCGGCGCACCTGATGCCAAGACCGGCAAGCGCAAGGCCATGTATGAGCCGGTGCACGGTTCTGCCCCTG
ACATCGCTGGCAAGAGCATTGCCAACCCGATCGCCATGATCGCGTCCTTCGCCATGTGCCTGCGCTACTCGTTCAACATG
GTGGACGAAGCCACCAAGCTCGAGGCGGCGATCGCCAATGTATTGGACAAGGGCATCCGCACCGCCGACATCATGGCCGA
CGGTTGCCGTCAGGTCGGCACGTCTGACATGGGTGACGCGGTTCTCGCCGAATTCAAGGCGCTTTCGGCGTAA

Upstream 100 bases:

>100_bases
CGACTTGGCATGCGCAGGACGGAACGAGTTTGACGCCGCGCTCGCCGCCATCGAAAACCCTTCGTGATGTTTTCCGGCGC
GCCGCCCAAGGAGGGTTCTC

Downstream 100 bases:

>100_bases
CGCTTAAAACGGGCTGGGCAGTTGGCGCCGCCGTCTGCCCGTCCTCCTTTGGCAGAAGATGCTCGTAACGACGGTCGGTC
AGCGTCTTCAGAAATGCCAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTVRSLFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDADMEKALAADAILFGAVGGPKW
DGVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASSLKPELVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGI
DTQIYDTFEIERIASVAFELARSRDNRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSIANPIAMIASFAMCLRYSFNM
VDEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDAVLAEFKALSA

Sequences:

>Translated_370_residues
MTVRSLFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDADMEKALAADAILFGAVGGPKW
DGVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASSLKPELVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGI
DTQIYDTFEIERIASVAFELARSRDNRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSIANPIAMIASFAMCLRYSFNM
VDEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDAVLAEFKALSA
>Mature_369_residues
TVRSLFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDADMEKALAADAILFGAVGGPKWD
GVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASSLKPELVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGID
TQIYDTFEIERIASVAFELARSRDNRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFDV
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSIANPIAMIASFAMCLRYSFNMV
DEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDAVLAEFKALSA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=367, Percent_Identity=32.1525885558583, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=30, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178816, Length=320, Percent_Identity=30, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4758582, Length=363, Percent_Identity=28.6501377410468, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI28178838, Length=342, Percent_Identity=28.9473684210526, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=37.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=50.4201680672269, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=33.9779005524862, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787381, Length=308, Percent_Identity=26.6233766233766, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=359, Percent_Identity=32.3119777158774, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=370, Percent_Identity=31.0810810810811, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=364, Percent_Identity=29.9450549450549, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=28.2798833819242, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=40.4825737265416, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=351, Percent_Identity=34.1880341880342, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324291, Length=378, Percent_Identity=25.3968253968254, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=32.0699708454811, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=32.0699708454811, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24661184, Length=374, Percent_Identity=29.9465240641711, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078637, Length=380, Percent_Identity=27.1052631578947, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078635, Length=380, Percent_Identity=27.1052631578947, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078633, Length=380, Percent_Identity=27.1052631578947, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650122, Length=380, Percent_Identity=27.1052631578947, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=376, Percent_Identity=27.3936170212766, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362242, Length=365, Percent_Identity=26.5753424657534, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648872, Length=365, Percent_Identity=26.5753424657534, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_AGRT5 (P24404)

Other databases:

- EMBL:   M38670
- EMBL:   AE007869
- PIR:   AF2919
- PIR:   G97693
- RefSeq:   NP_355719.1
- ProteinModelPortal:   P24404
- SMR:   P24404
- STRING:   P24404
- GeneID:   1134829
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2791
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   P24404
- ProtClustDB:   PRK00772
- BioCyc:   ATUM176299-1:ATU2791-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39659; Mature: 39528

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRSLFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDAD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCHH
MEKALAADAILFGAVGGPKWDGVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASS
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LKPELVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQIYDTFEIERIASVAFEL
CCHHHHCCCCEEEEEECCCCEEECCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ARSRDNRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFD
HHCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHH
ANPIAMIASFAMCLRYSFNMVDEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
VLAEFKALSA
HHHHHHHHCC
>Mature Secondary Structure 
TVRSLFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDAD
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCHH
MEKALAADAILFGAVGGPKWDGVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASS
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LKPELVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQIYDTFEIERIASVAFEL
CCHHHHCCCCEEEEEECCCCEEECCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ARSRDNRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFD
HHCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHH
ANPIAMIASFAMCLRYSFNMVDEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
VLAEFKALSA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194