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The map label for this gene is rlmH

Identifier: 15890025

GI number: 15890025

Start: 2781725

End: 2782207

Strand: Reverse

Name: rlmH

Synonym: Atu2776

Alternate gene names: 15890025

Gene position: 2782207-2781725 (Counterclockwise)

Preceding gene: 159185376

Following gene: 159185375

Centisome position: 97.91

GC content: 60.46

Gene sequence:

>483_bases
ATGCGGATTTCAATTTTTGCGGTGGGACGGCTGAAATCCGGCCCTGAAAAGGATCTTGCGGCCCGTTATATCGAGCGTCT
GGCCAAGACCGGCCCGGCGATCGGGCTGGAGTTTTCCCGGGTGATCGAGGTGGGCGAAAGCCGTGCTTCCAATGCCGAGA
CCCGCAAGCGCGAAGAAGCGGCGATGCTGGAAAAACATCTGGCTGACGGCGCAGTCCTCGTCTTGCTGGACGAGCGCGGG
AAAGCCCTTGATAGTCCTGCCTTCGCTTCGCTTTTTGGCGATCTGCGCGATAGCGGCAAGCGTGACCTCGTGATCGCCAT
AGGCGGGGCAGACGGGCTCGATTCCGCGCTTTACGACAAGGCGACGGCGGTTCTCAATCTCGGCAAGCTGACCTGGCCGC
ATCAGATCGTTCGTATCCTGATTGCCGAACAGCTCTACCGCGCCGTCACCATCCTTTCCGGCCATCCCTACCACCGCGTT
TGA

Upstream 100 bases:

>100_bases
AAAGGTGTCTCTGGCCGGAAATCAGACCGGCCGCATGGGAATCCGCAGGCTATGCTGTTAAGAGCGGGACCCTGTTGGTC
ACGACGAATGCAGGTTTGCG

Downstream 100 bases:

>100_bases
ACGGGTTTCGATTCCAAAAATCGTCTCAATTTCAACATGAAACTCCGCGACACCGCTGGCTGCGGGGGGCTTTGACGGCA
GCCTTTTTCAGGCCCCTTGG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRISIFAVGRLKSGPEKDLAARYIERLAKTGPAIGLEFSRVIEVGESRASNAETRKREEAAMLEKHLADGAVLVLLDERG
KALDSPAFASLFGDLRDSGKRDLVIAIGGADGLDSALYDKATAVLNLGKLTWPHQIVRILIAEQLYRAVTILSGHPYHRV

Sequences:

>Translated_160_residues
MRISIFAVGRLKSGPEKDLAARYIERLAKTGPAIGLEFSRVIEVGESRASNAETRKREEAAMLEKHLADGAVLVLLDERG
KALDSPAFASLFGDLRDSGKRDLVIAIGGADGLDSALYDKATAVLNLGKLTWPHQIVRILIAEQLYRAVTILSGHPYHRV
>Mature_160_residues
MRISIFAVGRLKSGPEKDLAARYIERLAKTGPAIGLEFSRVIEVGESRASNAETRKREEAAMLEKHLADGAVLVLLDERG
KALDSPAFASLFGDLRDSGKRDLVIAIGGADGLDSALYDKATAVLNLGKLTWPHQIVRILIAEQLYRAVTILSGHPYHRV

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=162, Percent_Identity=34.5679012345679, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_AGRT5 (Q8UBS4)

Other databases:

- EMBL:   AE007869
- PIR:   AG2917
- PIR:   B97692
- RefSeq:   NP_355706.1
- ProteinModelPortal:   Q8UBS4
- SMR:   Q8UBS4
- STRING:   Q8UBS4
- GeneID:   1134814
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2776
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- PhylomeDB:   Q8UBS4
- ProtClustDB:   PRK00103
- BioCyc:   ATUM176299-1:ATU2776-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17418; Mature: 17418

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISIFAVGRLKSGPEKDLAARYIERLAKTGPAIGLEFSRVIEVGESRASNAETRKREEA
CEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AMLEKHLADGAVLVLLDERGKALDSPAFASLFGDLRDSGKRDLVIAIGGADGLDSALYDK
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
ATAVLNLGKLTWPHQIVRILIAEQLYRAVTILSGHPYHRV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRISIFAVGRLKSGPEKDLAARYIERLAKTGPAIGLEFSRVIEVGESRASNAETRKREEA
CEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AMLEKHLADGAVLVLLDERGKALDSPAFASLFGDLRDSGKRDLVIAIGGADGLDSALYDK
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
ATAVLNLGKLTWPHQIVRILIAEQLYRAVTILSGHPYHRV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194