Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 15890010

GI number: 15890010

Start: 2762799

End: 2763602

Strand: Direct

Name: xthA [H]

Synonym: Atu2761

Alternate gene names: 15890010

Gene position: 2762799-2763602 (Clockwise)

Preceding gene: 159185367

Following gene: 159185369

Centisome position: 97.23

GC content: 55.97

Gene sequence:

>804_bases
ATGTCGTTTTCGATAACCACCTGGAACATCAACTCGGTCAGGCTCAGAATGCCAATCGTTGAGCAGTTTCTGGTGCGTTA
CAAACCCGACATTCTCTGCCTGCAGGAAACGAAATGCCCGAATGGCGAGTTTCCGATGAAACCGCTAAAAGCACTCGGTT
ACGAGCACGTCATCATCCATGGTCAAAAAGGCTATCATGGTGTGGCGATCGCTTCGAAAATCCCGCTGACCGAAGACCAC
CGGCAGGATTATTGCGGCATCGGCGATGCCAGGCATATTTCCGCGATTTTCCAGGTCGGATCGCGCCGCGTGCGGCTGCA
TAATTTCTACGTTCCGGCTGGCGGCGACGAGCCGGATCGGTCGATCAATCCGAAATTCGGCCACAAGCTCGATTTCATCG
AGGAAATGAAGGCACTGCGCGCCGATGGCGTGCCCGGGACCTCCTCCATTCTCGTCGGCGATCTCAACATTGCACCGCTT
GAAAATGACGTCTGGTCGCACAAGCAGCTTCTGAAGATCGTCAGCCACACGCCTGTCGAAACCGAAGGCATGCTGGATAT
CATGAAGAAGGGCAACTGGCTCGATCTGATGCGCCTGAACGTGCCGGAGACGGAAAAGATCTATACGTGGTGGAGCTACC
GCGCCAAGGATTGGGAAGCCGCTAACCGCGGCCGCCGTCTCGACCATATCTGGTCCTCACAGGACCTCGGTTCCTCGCTT
GAAAAAATCGAGATATTGCGTGAAGCCCGCGGCTGGAACCGGCCATCGGACCACGTCCCCGTCACGGCTCATTTCCGCTT
CTGA

Upstream 100 bases:

>100_bases
CGCTAAAATACATTCCCGTTTTCATCGCGATGTTATAAGCCTGCGCCCCGAAACCATACCTTGAGGCTCTACCTCGAGGC
GATAGAAACGGAGCCCATGC

Downstream 100 bases:

>100_bases
ACGCTAGAGCCGGCTTCAGACGAAGCGGTTCTGCAAACGGGCTGCACCCGCCGCCAGCGCCGCAATATTGTCGCGAATAC
GCGCCGACACGATATCGGCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSFSITTWNINSVRLRMPIVEQFLVRYKPDILCLQETKCPNGEFPMKPLKALGYEHVIIHGQKGYHGVAIASKIPLTEDH
RQDYCGIGDARHISAIFQVGSRRVRLHNFYVPAGGDEPDRSINPKFGHKLDFIEEMKALRADGVPGTSSILVGDLNIAPL
ENDVWSHKQLLKIVSHTPVETEGMLDIMKKGNWLDLMRLNVPETEKIYTWWSYRAKDWEAANRGRRLDHIWSSQDLGSSL
EKIEILREARGWNRPSDHVPVTAHFRF

Sequences:

>Translated_267_residues
MSFSITTWNINSVRLRMPIVEQFLVRYKPDILCLQETKCPNGEFPMKPLKALGYEHVIIHGQKGYHGVAIASKIPLTEDH
RQDYCGIGDARHISAIFQVGSRRVRLHNFYVPAGGDEPDRSINPKFGHKLDFIEEMKALRADGVPGTSSILVGDLNIAPL
ENDVWSHKQLLKIVSHTPVETEGMLDIMKKGNWLDLMRLNVPETEKIYTWWSYRAKDWEAANRGRRLDHIWSSQDLGSSL
EKIEILREARGWNRPSDHVPVTAHFRF
>Mature_266_residues
SFSITTWNINSVRLRMPIVEQFLVRYKPDILCLQETKCPNGEFPMKPLKALGYEHVIIHGQKGYHGVAIASKIPLTEDHR
QDYCGIGDARHISAIFQVGSRRVRLHNFYVPAGGDEPDRSINPKFGHKLDFIEEMKALRADGVPGTSSILVGDLNIAPLE
NDVWSHKQLLKIVSHTPVETEGMLDIMKKGNWLDLMRLNVPETEKIYTWWSYRAKDWEAANRGRRLDHIWSSQDLGSSLE
KIEILREARGWNRPSDHVPVTAHFRF

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=28.7272727272727, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=28.7272727272727, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=28.7272727272727, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788046, Length=273, Percent_Identity=32.6007326007326, Blast_Score=110, Evalue=1e-25,
Organism=Drosophila melanogaster, GI221330655, Length=277, Percent_Identity=29.2418772563177, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136678, Length=277, Percent_Identity=29.2418772563177, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30648; Mature: 30516

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSITTWNINSVRLRMPIVEQFLVRYKPDILCLQETKCPNGEFPMKPLKALGYEHVIIH
CCEEEEEEECCEEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEE
GQKGYHGVAIASKIPLTEDHRQDYCGIGDARHISAIFQVGSRRVRLHNFYVPAGGDEPDR
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
SINPKFGHKLDFIEEMKALRADGVPGTSSILVGDLNIAPLENDVWSHKQLLKIVSHTPVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCEECCCCCCHHHHHHHHHHHHCCCCC
TEGMLDIMKKGNWLDLMRLNVPETEKIYTWWSYRAKDWEAANRGRRLDHIWSSQDLGSSL
CHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCHHHHCCCHHHHHHH
EKIEILREARGWNRPSDHVPVTAHFRF
HHHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SFSITTWNINSVRLRMPIVEQFLVRYKPDILCLQETKCPNGEFPMKPLKALGYEHVIIH
CEEEEEEECCEEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEE
GQKGYHGVAIASKIPLTEDHRQDYCGIGDARHISAIFQVGSRRVRLHNFYVPAGGDEPDR
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
SINPKFGHKLDFIEEMKALRADGVPGTSSILVGDLNIAPLENDVWSHKQLLKIVSHTPVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCEECCCCCCHHHHHHHHHHHHCCCCC
TEGMLDIMKKGNWLDLMRLNVPETEKIYTWWSYRAKDWEAANRGRRLDHIWSSQDLGSSL
CHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCHHHHCCCHHHHHHH
EKIEILREARGWNRPSDHVPVTAHFRF
HHHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]