Click here to switch to the map view.

The map label for this gene is glnK [H]

Identifier: 15890006

GI number: 15890006

Start: 2757251

End: 2757601

Strand: Direct

Name: glnK [H]

Synonym: Atu2757

Alternate gene names: 15890006

Gene position: 2757251-2757601 (Clockwise)

Preceding gene: 159185364

Following gene: 159185365

Centisome position: 97.03

GC content: 56.98

Gene sequence:

>351_bases
ATGGGAAACCAGATGAAAATTGTGATGGCCATTATCAAGCCGTTCAAGCTGGATGAGGTGCGCGAAGCACTCACCGGCGT
CGGCATCCAGGGCCTGACCGTGACCGAGGTGAAGGGTTACGGACGTCAGAAGGGGCATACCGAGATCTATCGCGGCACGG
AATACGCGGTGAGCTTCCTTCCCAAGCTGAAGATCGAGATCGCAGTTCCATCCGACGTCGTCGACAAGGCCGTCGAGGCG
ATCGCGTCTGCGGCAAAGACCGGGCAGATCGGTGACGGCAAGATCTTTGTCTACTCTATCGAACAGGCCGTGCGCATTCG
TACCGGCGAAACCAACACAGAAGCGCTTTAA

Upstream 100 bases:

>100_bases
GTACCAAAACTGGCACGCCCTTTGAATGTATGTCCGCAGTCGCTGTTACTGATCTGCCAGCGGCAAGGAGAGTCGGCTCC
ATGCCGAGGACAAACAAAGG

Downstream 100 bases:

>100_bases
AGCACGGCTGACAGGGGAGTTTTCCAACTATGCAACTCAACAAGTTTTTGACGCTCGGCAAGCTGGGCGCCGCAGCAACG
GCCCTGATGGCCCCGGCCAT

Product: nitrogen regulatory protein PII

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MGNQMKIVMAIIKPFKLDEVREALTGVGIQGLTVTEVKGYGRQKGHTEIYRGTEYAVSFLPKLKIEIAVPSDVVDKAVEA
IASAAKTGQIGDGKIFVYSIEQAVRIRTGETNTEAL

Sequences:

>Translated_116_residues
MGNQMKIVMAIIKPFKLDEVREALTGVGIQGLTVTEVKGYGRQKGHTEIYRGTEYAVSFLPKLKIEIAVPSDVVDKAVEA
IASAAKTGQIGDGKIFVYSIEQAVRIRTGETNTEAL
>Mature_115_residues
GNQMKIVMAIIKPFKLDEVREALTGVGIQGLTVTEVKGYGRQKGHTEIYRGTEYAVSFLPKLKIEIAVPSDVVDKAVEAI
ASAAKTGQIGDGKIFVYSIEQAVRIRTGETNTEAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=66.0714285714286, Blast_Score=157, Evalue=1e-40,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=61.6071428571429, Blast_Score=154, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12569; Mature: 12438

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQMKIVMAIIKPFKLDEVREALTGVGIQGLTVTEVKGYGRQKGHTEIYRGTEYAVSFL
CCCCCEEHEEHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHHC
PKLKIEIAVPSDVVDKAVEAIASAAKTGQIGDGKIFVYSIEQAVRIRTGETNTEAL
CCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCCCCCC
>Mature Secondary Structure 
GNQMKIVMAIIKPFKLDEVREALTGVGIQGLTVTEVKGYGRQKGHTEIYRGTEYAVSFL
CCCCEEHEEHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHHC
PKLKIEIAVPSDVVDKAVEAIASAAKTGQIGDGKIFVYSIEQAVRIRTGETNTEAL
CCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA