Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 15889987

GI number: 15889987

Start: 2734423

End: 2735409

Strand: Reverse

Name: galE [C]

Synonym: Atu2738

Alternate gene names: 15889987

Gene position: 2735409-2734423 (Counterclockwise)

Preceding gene: 159185358

Following gene: 15889986

Centisome position: 96.26

GC content: 61.09

Gene sequence:

>987_bases
ATGCATATCGCAATCATCGGCGCCGCAGGCATGGTTGGCCGCAAGCTGACGCAGCGTCTCGTCAAGGACGGATCGCTCGG
CGGCAAGCCGGTCGAGAAGTTCACGCTCATCGACGTGTTCCAGCCTGAAGCTCCCGCCGGTTTTTCCGGTGCAGTCGACG
CGCGGGCGGCCGATCTCTCGGCGCCGGGCGAAGCCGAAAAGCTGGTCGAAGCCCGTCCCGATGTCATCTTCCATCTTGCG
GCCATCGTCTCCGGTGAGGCCGAGCTTGATTTCGACAAGGGCTACCGCATCAATCTCGATGGCACCCGTTATCTCTTCGA
CGCCATCCGCATTGCCAATGGCAAGGATGGCTACAAGCCGCGCGTGGTCTTCACCTCGTCGATTGCCGTTTTTGGCGCGC
CGCTTCCCTATCCGATCCCGGATGAATTCCACACCACGCCGCTGACAAGCTATGGCACACAGAAGGCAATCTGCGAGCTA
CTGCTGTCCGACTACAGCCGCCGCGGTTTCTTTGACGGTATCGGCATTCGTCTGCCGACAATCTGCATTCGTCCCGGCAA
GCCCAATGCTGCCGCCTCCGGCTTCTTCTCCAATATCCTGCGCGAGCCGCTGGTCGGCCAGGAAGCGGTTCTGCCGGTTC
CGGAGAGCATTCGCCACTGGCATGCCTCGCCGCGTTCCGCCGTCGGTTTCCTTATCCATGGTGCGATGATCGACGTGGAA
AAGGTCGGCCCGCGCCGCAACCTTTCCATGCCGGGCCTCAGCGCCACCGTTGGCGAACAGATCGAGGCGCTGCGCAAGGT
TGCCGGTGAAAAGGCCGTTGCGCTCATCCGCCGTGAACCGAATGAGATGATCATGCGCATGTGCGAAGGCTGGGCACCCG
GCTTCGAAGCAAAGCGCGCCCGCGAGCTCGGCTTTACGGCCGAGAGCTCGTTTGAAGAGATCATTCAGGTTCATATTGAG
GACGAACTGGGAGGTTCACTGAAATGA

Upstream 100 bases:

>100_bases
TATAAGGTTATCAGACAACCTTACAAGCTAATTTATCGGTCACGAGGCCGATGGTGATTTTCGGTCCCGGGAGAATGGCC
GGGACATAGAGGAGGAAGAC

Downstream 100 bases:

>100_bases
GTGTCGGCGGGGAGGAGAAGAAGCGGTCTGTCGCCTTTATCGGCACGGGCCTGATGGGCGGGCCGATGGCCCGGCGTTTG
CTGGGTACCGGCTTTTCGGT

Product: nucleoside-diphosphate-sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MHIAIIGAAGMVGRKLTQRLVKDGSLGGKPVEKFTLIDVFQPEAPAGFSGAVDARAADLSAPGEAEKLVEARPDVIFHLA
AIVSGEAELDFDKGYRINLDGTRYLFDAIRIANGKDGYKPRVVFTSSIAVFGAPLPYPIPDEFHTTPLTSYGTQKAICEL
LLSDYSRRGFFDGIGIRLPTICIRPGKPNAAASGFFSNILREPLVGQEAVLPVPESIRHWHASPRSAVGFLIHGAMIDVE
KVGPRRNLSMPGLSATVGEQIEALRKVAGEKAVALIRREPNEMIMRMCEGWAPGFEAKRARELGFTAESSFEEIIQVHIE
DELGGSLK

Sequences:

>Translated_328_residues
MHIAIIGAAGMVGRKLTQRLVKDGSLGGKPVEKFTLIDVFQPEAPAGFSGAVDARAADLSAPGEAEKLVEARPDVIFHLA
AIVSGEAELDFDKGYRINLDGTRYLFDAIRIANGKDGYKPRVVFTSSIAVFGAPLPYPIPDEFHTTPLTSYGTQKAICEL
LLSDYSRRGFFDGIGIRLPTICIRPGKPNAAASGFFSNILREPLVGQEAVLPVPESIRHWHASPRSAVGFLIHGAMIDVE
KVGPRRNLSMPGLSATVGEQIEALRKVAGEKAVALIRREPNEMIMRMCEGWAPGFEAKRARELGFTAESSFEEIIQVHIE
DELGGSLK
>Mature_328_residues
MHIAIIGAAGMVGRKLTQRLVKDGSLGGKPVEKFTLIDVFQPEAPAGFSGAVDARAADLSAPGEAEKLVEARPDVIFHLA
AIVSGEAELDFDKGYRINLDGTRYLFDAIRIANGKDGYKPRVVFTSSIAVFGAPLPYPIPDEFHTTPLTSYGTQKAICEL
LLSDYSRRGFFDGIGIRLPTICIRPGKPNAAASGFFSNILREPLVGQEAVLPVPESIRHWHASPRSAVGFLIHGAMIDVE
KVGPRRNLSMPGLSATVGEQIEALRKVAGEKAVALIRREPNEMIMRMCEGWAPGFEAKRARELGFTAESSFEEIIQVHIE
DELGGSLK

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35535; Mature: 35535

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIAIIGAAGMVGRKLTQRLVKDGSLGGKPVEKFTLIDVFQPEAPAGFSGAVDARAADLS
CEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
APGEAEKLVEARPDVIFHLAAIVSGEAELDFDKGYRINLDGTRYLFDAIRIANGKDGYKP
CCCHHHHHHHCCCHHHHHHHHHHCCCCCEECCCCEEEECCCHHHHHHHHHHCCCCCCCCC
RVVFTSSIAVFGAPLPYPIPDEFHTTPLTSYGTQKAICELLLSDYSRRGFFDGIGIRLPT
EEEEEECHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
ICIRPGKPNAAASGFFSNILREPLVGQEAVLPVPESIRHWHASPRSAVGFLIHGAMIDVE
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHEEEHH
KVGPRRNLSMPGLSATVGEQIEALRKVAGEKAVALIRREPNEMIMRMCEGWAPGFEAKRA
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
RELGFTAESSFEEIIQVHIEDELGGSLK
HHCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHIAIIGAAGMVGRKLTQRLVKDGSLGGKPVEKFTLIDVFQPEAPAGFSGAVDARAADLS
CEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
APGEAEKLVEARPDVIFHLAAIVSGEAELDFDKGYRINLDGTRYLFDAIRIANGKDGYKP
CCCHHHHHHHCCCHHHHHHHHHHCCCCCEECCCCEEEECCCHHHHHHHHHHCCCCCCCCC
RVVFTSSIAVFGAPLPYPIPDEFHTTPLTSYGTQKAICELLLSDYSRRGFFDGIGIRLPT
EEEEEECHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
ICIRPGKPNAAASGFFSNILREPLVGQEAVLPVPESIRHWHASPRSAVGFLIHGAMIDVE
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHEEEHH
KVGPRRNLSMPGLSATVGEQIEALRKVAGEKAVALIRREPNEMIMRMCEGWAPGFEAKRA
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
RELGFTAESSFEEIIQVHIEDELGGSLK
HHCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]