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The map label for this gene is gcvA [H]

Identifier: 15889817

GI number: 15889817

Start: 2531299

End: 2532225

Strand: Reverse

Name: gcvA [H]

Synonym: Atu2559

Alternate gene names: 15889817

Gene position: 2532225-2531299 (Counterclockwise)

Preceding gene: 15889822

Following gene: 159185266

Centisome position: 89.11

GC content: 61.38

Gene sequence:

>927_bases
ATGGTTCTGCCGCCGCGCCGTTTTCTGCCGTCGCTTTCGCTGCTGGCGGCTTTCGAGGCTGCATCGCGCACCGGCAGCGT
TACCGCAGCGGCAAGGGAGCTCGGCCTGACACAGGGTGCGGTCAGCCGGCAGATTCTGGCGCTTGAAGAGCAGCTTGGTG
TGGCACTGTTCCTGCGTGAACGGCAGACCATCCGCTTGACCCGGGCGGGGGAAGGTTACGCGCGGGAAATCCGCGAGGCT
TTGCGGCGTATTTCGACGGCGTCCCTCAATCTGCGCGCCAATCCGGATGGTGGCACGCTCAATCTCGGCGTGCTGCCCAG
CTTCGGCACACGCTGGCTGGTGCCCCGCCTTCCGGATTTCATCGCCCGGCATCCCGGCATTTCGGTCAATCTTCTGACGC
GGTCGTCGCTGTTCGATTTCCGCACCGATACCGTCGATGCGGCGATCCATTTCGGTTTGCCGCATTGGCCGGGCACCGAA
CTGGCTTTCCTGATGCATGAAAAGGTCATTCCGGTGTGTAGCCCGGCTTTCAAGGAACTTTACGGGCTTTCTCGGCCGGA
TGATCTGCTGCATGTACCACTCCTGCACATGACAACCCGGCCGGACGCCTGGGAACAATGGTTTCGCAGCCATGATGTGC
GTTTCGACAATGTGCACGGCATGCTGTTCGATCAGTTCACGACGATTGCCGAGGCGTCGAGCGCTGGCGTCGGGGCTTCG
CTTGTGCCGTCCTTCATGATCGAGGAGGAATTGCGAAGCGGACGTCTGGTCTCAGCCGTTGCGGGGGAAGTGGAAAGCGA
AGAGGCCTATTATCTTGCATGCATGCCGGACCGCGCGACCTATCCGCCCCTTGAAAGTTTCCGGCGCTGGATCGTTTATC
AGGCCGCAATCGGCAGGGATGTCGCCGGCGAAGACTTTTCGGCGTAA

Upstream 100 bases:

>100_bases
GATTTGCGTATGAGTGACAAGCCCTTGTTTATGAGACTTTACAAGCCAGTCTGTCTGTTGAATATCGACATATCGTCAAG
AGTTCATTCCGTGAGGGAAT

Downstream 100 bases:

>100_bases
ATCGGGGACGGTCTTGCAAGAGCGCCGCGGCTAAGCCAATAGTGCGCCATGACGTGTGGCGGACGGCGCCACGCACCGCA
AATTCCAATACGGGAGGTGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVLPPRRFLPSLSLLAAFEAASRTGSVTAAARELGLTQGAVSRQILALEEQLGVALFLRERQTIRLTRAGEGYAREIREA
LRRISTASLNLRANPDGGTLNLGVLPSFGTRWLVPRLPDFIARHPGISVNLLTRSSLFDFRTDTVDAAIHFGLPHWPGTE
LAFLMHEKVIPVCSPAFKELYGLSRPDDLLHVPLLHMTTRPDAWEQWFRSHDVRFDNVHGMLFDQFTTIAEASSAGVGAS
LVPSFMIEEELRSGRLVSAVAGEVESEEAYYLACMPDRATYPPLESFRRWIVYQAAIGRDVAGEDFSA

Sequences:

>Translated_308_residues
MVLPPRRFLPSLSLLAAFEAASRTGSVTAAARELGLTQGAVSRQILALEEQLGVALFLRERQTIRLTRAGEGYAREIREA
LRRISTASLNLRANPDGGTLNLGVLPSFGTRWLVPRLPDFIARHPGISVNLLTRSSLFDFRTDTVDAAIHFGLPHWPGTE
LAFLMHEKVIPVCSPAFKELYGLSRPDDLLHVPLLHMTTRPDAWEQWFRSHDVRFDNVHGMLFDQFTTIAEASSAGVGAS
LVPSFMIEEELRSGRLVSAVAGEVESEEAYYLACMPDRATYPPLESFRRWIVYQAAIGRDVAGEDFSA
>Mature_308_residues
MVLPPRRFLPSLSLLAAFEAASRTGSVTAAARELGLTQGAVSRQILALEEQLGVALFLRERQTIRLTRAGEGYAREIREA
LRRISTASLNLRANPDGGTLNLGVLPSFGTRWLVPRLPDFIARHPGISVNLLTRSSLFDFRTDTVDAAIHFGLPHWPGTE
LAFLMHEKVIPVCSPAFKELYGLSRPDDLLHVPLLHMTTRPDAWEQWFRSHDVRFDNVHGMLFDQFTTIAEASSAGVGAS
LVPSFMIEEELRSGRLVSAVAGEVESEEAYYLACMPDRATYPPLESFRRWIVYQAAIGRDVAGEDFSA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=37.6712328767123, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=31.740614334471, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=32.3024054982818, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=28.8461538461538, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786401, Length=279, Percent_Identity=26.1648745519713, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=35, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=26, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787128, Length=254, Percent_Identity=27.9527559055118, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34161; Mature: 34161

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPPRRFLPSLSLLAAFEAASRTGSVTAAARELGLTQGAVSRQILALEEQLGVALFLRE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEC
RQTIRLTRAGEGYAREIREALRRISTASLNLRANPDGGTLNLGVLPSFGTRWLVPRLPDF
CCEEEEEECCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCEECCCCCHHH
IARHPGISVNLLTRSSLFDFRTDTVDAAIHFGLPHWPGTELAFLMHEKVIPVCSPAFKEL
HHHCCCCEEEEEECCHHHHCCCCHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
YGLSRPDDLLHVPLLHMTTRPDAWEQWFRSHDVRFDNVHGMLFDQFTTIAEASSAGVGAS
HCCCCCCCCEECCHHEECCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCCHH
LVPSFMIEEELRSGRLVSAVAGEVESEEAYYLACMPDRATYPPLESFRRWIVYQAAIGRD
HHHHHHHHHHHHCCCEEHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
VAGEDFSA
CCCCCCCC
>Mature Secondary Structure
MVLPPRRFLPSLSLLAAFEAASRTGSVTAAARELGLTQGAVSRQILALEEQLGVALFLRE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEC
RQTIRLTRAGEGYAREIREALRRISTASLNLRANPDGGTLNLGVLPSFGTRWLVPRLPDF
CCEEEEEECCCHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCEECCCCCHHH
IARHPGISVNLLTRSSLFDFRTDTVDAAIHFGLPHWPGTELAFLMHEKVIPVCSPAFKEL
HHHCCCCEEEEEECCHHHHCCCCHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
YGLSRPDDLLHVPLLHMTTRPDAWEQWFRSHDVRFDNVHGMLFDQFTTIAEASSAGVGAS
HCCCCCCCCEECCHHEECCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCCHH
LVPSFMIEEELRSGRLVSAVAGEVESEEAYYLACMPDRATYPPLESFRRWIVYQAAIGRD
HHHHHHHHHHHHCCCEEHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
VAGEDFSA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]