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The map label for this gene is mnmC [H]

Identifier: 15889608

GI number: 15889608

Start: 2305705

End: 2306460

Strand: Direct

Name: mnmC [H]

Synonym: Atu2331

Alternate gene names: 15889608

Gene position: 2305705-2306460 (Clockwise)

Preceding gene: 159185157

Following gene: 15889610

Centisome position: 81.14

GC content: 62.96

Gene sequence:

>756_bases
ATGAAAAATTCCAACGACACTATCAGAGAAACTGGCGCCGAGACCGGCTCGGCAACCGGGCAAAACCAGACGGCGCTCGA
CTGGCGTGACGGGGATATGCCCTATTCGCGGGCCTTTGACGATCATTTTTATTGCCAGACGGATGGCAGGCTGGAATGCG
GCCATGTCTTCCTGTCCGGAAACGGGTTGCCGCAACGCTGGCTCGAACGGGACGGTGTTTTCCGCATCGGCGAGCTGGGC
TTCGGCACCGGCCTCAATCTCTGCGAGACCTGGCGGCAATGGAAAGAGGTCCGCAACGGCCGCTCCACGCTGCATTTCGT
GTCCTTCGAACTTTACCCGATGAAAGCGGACGAGATCGACCGGGCGCTTTCCCGCTGGCCGGAAGTGGATGCTGAGCGCA
AGGCGCTTGTCGCCCGCTGGCCCGCCGAGCCGCAGGGCCGGGTCGAGATCGAACTGGACGAAGCAACGCGCCTGACGGTG
GTCTGCGGCGAGGCGCTTGCCGGCATTTCCGGCAGCGATCTGAGTTTCGACGCCTGGTTTCTCGATGGTTTCGCGCCCGC
GCGCAACCCGGACATGTGGTCGCTGGAGATCATGCAGGCGCTGTTTGCGAAAACGGCACCGGGGGGCAGATTCGCCACCT
ACGCCGCCGCCGGCTTCGTCCGCCGCAATCTCATCGCAGCCGGTTTTGACCTGGAGCGCAGAAAGGGATTTGCCGGAAAG
CGGGAAATGCTCTGCGGCACCAAACCCTCGGCCTGA

Upstream 100 bases:

>100_bases
ACCCATGATGCCGCCGCCGACGATGAGGAGCGGCACGGAATCCGGCAAACGCATTCTTGGTGATTGACCCTGTTCGAAAG
CGGGATTATCGGCTTTAGCC

Downstream 100 bases:

>100_bases
TGCAATTCAGAAAATGACGCCGGATATTCACACGCCCGGAGCATCCCGCCGCAGCACGTTCTCGCCCAGCCATTGCGCGA
TCTTGTCTTCGAGGCTTTCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKNSNDTIRETGAETGSATGQNQTALDWRDGDMPYSRAFDDHFYCQTDGRLECGHVFLSGNGLPQRWLERDGVFRIGELG
FGTGLNLCETWRQWKEVRNGRSTLHFVSFELYPMKADEIDRALSRWPEVDAERKALVARWPAEPQGRVEIELDEATRLTV
VCGEALAGISGSDLSFDAWFLDGFAPARNPDMWSLEIMQALFAKTAPGGRFATYAAAGFVRRNLIAAGFDLERRKGFAGK
REMLCGTKPSA

Sequences:

>Translated_251_residues
MKNSNDTIRETGAETGSATGQNQTALDWRDGDMPYSRAFDDHFYCQTDGRLECGHVFLSGNGLPQRWLERDGVFRIGELG
FGTGLNLCETWRQWKEVRNGRSTLHFVSFELYPMKADEIDRALSRWPEVDAERKALVARWPAEPQGRVEIELDEATRLTV
VCGEALAGISGSDLSFDAWFLDGFAPARNPDMWSLEIMQALFAKTAPGGRFATYAAAGFVRRNLIAAGFDLERRKGFAGK
REMLCGTKPSA
>Mature_251_residues
MKNSNDTIRETGAETGSATGQNQTALDWRDGDMPYSRAFDDHFYCQTDGRLECGHVFLSGNGLPQRWLERDGVFRIGELG
FGTGLNLCETWRQWKEVRNGRSTLHFVSFELYPMKADEIDRALSRWPEVDAERKALVARWPAEPQGRVEIELDEATRLTV
VCGEALAGISGSDLSFDAWFLDGFAPARNPDMWSLEIMQALFAKTAPGGRFATYAAAGFVRRNLIAAGFDLERRKGFAGK
REMLCGTKPSA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=225, Percent_Identity=41.7777777777778, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 28027; Mature: 28027

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSNDTIRETGAETGSATGQNQTALDWRDGDMPYSRAFDDHFYCQTDGRLECGHVFLSG
CCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCEEEECCCCEEEEEEEEEC
NGLPQRWLERDGVFRIGELGFGTGLNLCETWRQWKEVRNGRSTLHFVSFELYPMKADEID
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHH
RALSRWPEVDAERKALVARWPAEPQGRVEIELDEATRLTVVCGEALAGISGSDLSFDAWF
HHHHHCCCCCCCHHEEEEECCCCCCCCEEEEECCCEEEEEEECHHHCCCCCCCCCEEEEE
LDGFAPARNPDMWSLEIMQALFAKTAPGGRFATYAAAGFVRRNLIAAGFDLERRKGFAGK
ECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
REMLCGTKPSA
HHHCCCCCCCC
>Mature Secondary Structure
MKNSNDTIRETGAETGSATGQNQTALDWRDGDMPYSRAFDDHFYCQTDGRLECGHVFLSG
CCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCEEEECCCCEEEEEEEEEC
NGLPQRWLERDGVFRIGELGFGTGLNLCETWRQWKEVRNGRSTLHFVSFELYPMKADEID
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHH
RALSRWPEVDAERKALVARWPAEPQGRVEIELDEATRLTVVCGEALAGISGSDLSFDAWF
HHHHHCCCCCCCHHEEEEECCCCCCCCEEEEECCCEEEEEEECHHHCCCCCCCCCEEEEE
LDGFAPARNPDMWSLEIMQALFAKTAPGGRFATYAAAGFVRRNLIAAGFDLERRKGFAGK
ECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
REMLCGTKPSA
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA