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The map label for this gene is greA [H]

Identifier: 15889462

GI number: 15889462

Start: 2158796

End: 2159272

Strand: Direct

Name: greA [H]

Synonym: Atu2182

Alternate gene names: 15889462

Gene position: 2158796-2159272 (Clockwise)

Preceding gene: 15889458

Following gene: 15889463

Centisome position: 75.97

GC content: 58.28

Gene sequence:

>477_bases
ATGGTAGAAAAAGTACCGATGACTCAGGGTGGATTCGTCAAGCTGCAGGAGGAGCTGCGCTTTCGCCAGCAGGAGGAGCG
TCCCCGTATCATCGAGGCAATTGCGGAAGCGCGCGCCCATGGCGACCTTTCGGAAAATGCCGAGTACCATGCTGCCAAGG
AAGCACAGAGCCACAATGAAGGCCGCATCACCGAACTCGAAGACCTGACCGCGCGCGCGGAAGTCATCGATCTTTCCAAG
ATGTCCGGTTCCAAGATCAAGTTCGGCGCCACCGTCAAGCTCATCGATGAAGACAGCGACGAGGAAAAGACCTATCAGAT
CGTCGGCGATCAGGAAGCCGATGTGAAGGCCGGCCGCATTTCCATCTCCTCGCCGATTGCCCGCGCCCTCATCGGCAAGG
AAGTCGGCGACAGCATAGAAGTCAACGCGCCGGGTGGTGCCAAGGGTTACGAGATTCTCGCAGTTCAGTGGGGCTGA

Upstream 100 bases:

>100_bases
CGGCTCAGTTCTGATTTTGGCATGGTTCCGGAGTTCCTCTCCGGAACCTGTTTCTTTTTGTGTCTGCAAGTAAAGCGACC
GGAATACGAAGGATAAAAAA

Downstream 100 bases:

>100_bases
TTGGCCCGATGCGATGCGCCTCGAGCGGGCGCATCGCTCCCTGACGAATCAACCTTCAGCCGCAGAGACGGAAGCCCATC
TTGTCCCACGTCAGTCTGAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MVEKVPMTQGGFVKLQEELRFRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSHNEGRITELEDLTARAEVIDLSK
MSGSKIKFGATVKLIDEDSDEEKTYQIVGDQEADVKAGRISISSPIARALIGKEVGDSIEVNAPGGAKGYEILAVQWG

Sequences:

>Translated_158_residues
MVEKVPMTQGGFVKLQEELRFRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSHNEGRITELEDLTARAEVIDLSK
MSGSKIKFGATVKLIDEDSDEEKTYQIVGDQEADVKAGRISISSPIARALIGKEVGDSIEVNAPGGAKGYEILAVQWG
>Mature_158_residues
MVEKVPMTQGGFVKLQEELRFRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSHNEGRITELEDLTARAEVIDLSK
MSGSKIKFGATVKLIDEDSDEEKTYQIVGDQEADVKAGRISISSPIARALIGKEVGDSIEVNAPGGAKGYEILAVQWG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=52.8662420382166, Blast_Score=163, Evalue=5e-42,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=34.8387096774194, Blast_Score=93, Evalue=8e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17308; Mature: 17308

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKVPMTQGGFVKLQEELRFRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSHNE
CCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GRITELEDLTARAEVIDLSKMSGSKIKFGATVKLIDEDSDEEKTYQIVGDQEADVKAGRI
CCCCHHHHHHHHHHHEEHHCCCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCEECEE
SISSPIARALIGKEVGDSIEVNAPGGAKGYEILAVQWG
ECCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MVEKVPMTQGGFVKLQEELRFRQQEERPRIIEAIAEARAHGDLSENAEYHAAKEAQSHNE
CCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GRITELEDLTARAEVIDLSKMSGSKIKFGATVKLIDEDSDEEKTYQIVGDQEADVKAGRI
CCCCHHHHHHHHHHHEEHHCCCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCEECEE
SISSPIARALIGKEVGDSIEVNAPGGAKGYEILAVQWG
ECCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA