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The map label for this gene is dnaG [H]

Identifier: 15889447

GI number: 15889447

Start: 2136980

End: 2138965

Strand: Reverse

Name: dnaG [H]

Synonym: Atu2168

Alternate gene names: 15889447

Gene position: 2138965-2136980 (Counterclockwise)

Preceding gene: 15889448

Following gene: 15889446

Centisome position: 75.27

GC content: 61.58

Gene sequence:

>1986_bases
ATGCGCTTTTCCAATTCTTTTCTCGATGAGATACGCGACCGCGTGAACATCTCCGATGTGATCGGCCGACGTGTGTCCTG
GGATAAGAAAAAGACCAATGCGCCGAGGGGGGATTATTGGGCCTGCTGCCCGTTCCATGGCGAGAAAAGCCCGAGCTTTC
ACTGCGAAGACCGCAAGGGGCGCTATCATTGTTTTGGTTGCGGCGTTTCTGGCGATCATTTCCGCTTTCTGACGGAGCTT
GAGGGGCTGAGTTTTCCCGAAGCCGTGCAGCAGATTGCCGATATGGCCGGCATTTCCATGCCGCAGCCCGATCCGCAGGC
GGAAAAGCGCGAGCGCGAGCGCACCTCGCTGCAGGACGTCATGGAAATGGCGACGCTGTTCTTTCAGGATCAGTTGCAGA
CCTCGCTCGGCGCGCGGGCGCGGGCTTACCTTCGGGATCGCGGGCTGACCGGGCGCACCATCGAGACCTTCCGGCTGGGT
TATGCGCCTGACAGCCGCAATGCACTGAAGGAGCATCTTGCAAGCAAGGGCGTGTCCAAGGAGCAGATGGAAGCCTGCGG
GCTGGTGGTGCATGAAAACGTGCCGGTTTCCTACGACCGGTTCCGCGACCGCATCATGTTCCCGATCCTGTCGTCGCGGG
AAAAAGTGATCGCCTTTGGCGGCCGCGCCATGGCGGCGGATGCGGTGGCGAAATATCTCAATTCCAACGAGACCGAACTC
TTTCACAAGGGCAATGTTCTCTACAATTTCGCCCGCGCGCGCCGCGCTTCGCAGGCGGCCGGCGGCACTGTTATCGCCGT
CGAAGGTTATATGGATGTCATCGCGCTTTATCAGGCGGGTGTCGAAAATGCTGTCGCGCCGCTCGGGACGGCGTTGACCG
ACAGCCAGCTCGATCTCCTCTGGAAGATGTCGCCGCAGCCGGTTCTCTGCTTCGATGGGGATGGCGCCGGCATTCGTGCC
GCCAACCGTGCGGCTGATCTTGCCCTGCCGCATATCAAGCCGGATCGTTCCGTCAGCTTTGCCCTGTTGCCTGACGGCAA
GGATCCGGATGATCTTGTGCGGCTGGAGGGGCGCGCGCCCTTTGACCGGGTGCTTTCGGAAGCCAAGCCGCTTGCCGCGA
TGATCTGGAGCCGCGAGACATCAGGCGTCACGTTCGATACGCCTGAAAAACGGGCTCAGCTTGAAAGCCGCCTGCGTCAG
ATCGTTACCGTGATTGCCGACGAGACGGTGCGCCGCTTTTACCAGCAGGATATGCGCGACAGGCTGAACGGCTTCTTCCA
GCCGCGCTTTCAGCAGGGCGGCTCGCAGGGCGGGAATTTCCGCCGCGACGGCGGTCAGGGTGCCGGTCGCGGTTTCCAGC
GCAATGCGCCGGGGCGGGGCGGGGCGATGTCTGCGCCGGCCAGCAGCATTTCCGACCGGCTGACGCAATCCGGTCTCGTC
AGGGGGCATCAGACCAAGCCGTCTCTGCGCGAAAGCGTTCTTGCCATCACCATCGTCAACCATCCCGAGTTGCTGCTGGA
GGAATATGACGAGATCGCCGCCATCGATTACGAGAACCGCGATCTGCAGCGGCTGTGGTCGACGGTGTTGACCTATGCGG
CCGAGAGTGCAGCGGACATATCGCGGGCAGGGCTGATCGACCGGCTGTCGCAGCACGGTTTCGAGCCGTTGTTGAAGACC
ATGGATCAGCAGGTGCGCAATGCGCGCCTGTGGACGGCGACGGAACAGGCGGCGATGGAGGATGCGCGCGAAGGTTACGT
GCAGGCACTTTCGCTGCATAAGAGAACCAAATCCCTGCTCTGGCAGAAACGGGAGCTGGAGCGGGAAATCGCCGAGGCGA
CCGATGATGAGCGTGGCACGCTTCTGGTGCGGGCGATTGCCGAAGTTCAATTGGAAATCGGCCGCATGGAAAATCAGGAA
GCGATCATCGATGGTTTCGGGGTCATGTCGGGCCGGGTGAAGGGTCCGGCCTTTGGCCATGGCTGA

Upstream 100 bases:

>100_bases
TTATCTCTGTGGCCTGTGAATATCGGGTATTGTCTTTCTTTGGGTTGGTGCTCAAGCCTTTCTGCGCTTATATGAAAGAC
TGGCCTCGAACAGGTAACCC

Downstream 100 bases:

>100_bases
GAGGCTGGCCAAGAATAGAAAGACGTGTGGGGTTGCCTTTTGCGGCAAGCGTCCTAAATAGGGGATAGTCTGGTGATAAC
CGCGAAATGCGGCCTTTTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDVMEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLG
YAPDSRNALKEHLASKGVSKEQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLLWKMSPQPVLCFDGDGAGIRA
ANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAPFDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQ
IVTVIADETVRRFYQQDMRDRLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADISRAGLIDRLSQHGFEPLLKT
MDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLLWQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQE
AIIDGFGVMSGRVKGPAFGHG

Sequences:

>Translated_661_residues
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDVMEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLG
YAPDSRNALKEHLASKGVSKEQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLLWKMSPQPVLCFDGDGAGIRA
ANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAPFDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQ
IVTVIADETVRRFYQQDMRDRLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADISRAGLIDRLSQHGFEPLLKT
MDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLLWQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQE
AIIDGFGVMSGRVKGPAFGHG
>Mature_661_residues
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDVMEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLG
YAPDSRNALKEHLASKGVSKEQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLLWKMSPQPVLCFDGDGAGIRA
ANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAPFDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQ
IVTVIADETVRRFYQQDMRDRLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADISRAGLIDRLSQHGFEPLLKT
MDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLLWQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQE
AIIDGFGVMSGRVKGPAFGHG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=425, Percent_Identity=37.4117647058824, Blast_Score=298, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 73633; Mature: 73633

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHHCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELEGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLGYAPDSRNALKEHLASKGVSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCHHHHHHHHHHCCCCH
EQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEE
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLL
EECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHH
WKMSPQPVLCFDGDGAGIRAANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAP
EECCCCCEEEECCCCCCCCHHCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHEEECCCCC
FDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQIVTVIADETVRRFYQQDMRD
HHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADI
CCCCCCHHHHHCEEEEEEECCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
SRAGLIDRLSQHGFEPLLKTMDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQEAIIDGFGVMSGRVKGPAFGH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHHCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELEGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLGYAPDSRNALKEHLASKGVSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCHHHHHHHHHHCCCCH
EQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEE
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLL
EECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHH
WKMSPQPVLCFDGDGAGIRAANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAP
EECCCCCEEEECCCCCCCCHHCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHEEECCCCC
FDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQIVTVIADETVRRFYQQDMRD
HHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADI
CCCCCCHHHHHCEEEEEEECCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
SRAGLIDRLSQHGFEPLLKTMDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQEAIIDGFGVMSGRVKGPAFGH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]