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The map label for this gene is tphA [H]

Identifier: 15889435

GI number: 15889435

Start: 2118701

End: 2120032

Strand: Direct

Name: tphA [H]

Synonym: Atu2154

Alternate gene names: 15889435

Gene position: 2118701-2120032 (Clockwise)

Preceding gene: 15889434

Following gene: 159185078

Centisome position: 74.56

GC content: 62.01

Gene sequence:

>1332_bases
ATGTCTCCCACCACCACCCCGGCGGCGGCCCGTCCCCTCAACAGCCAGGATTTCAGAACGCTCGGTCTTTCGGCCCTTGG
CGGCGCGCTCGAATTTTACGACTTCATCATTTTCGTATTTTTCGCCACCGTCATCGGCCATCTGTTTTTCCCGCCGGAAA
TGCCGGACTGGCTGGTGATGATTCAGACCTTCGGCATCTTCGCCGCCGGTTATCTGGTGCGGCCGCTCGGCGGCATCGTG
CTCGCCCATTATGGCGACAGGTATGGACGCAAGCGCGTCTTCGCCTTCTCCATCCTCCTGATGGCGCTCTCCACACTCGG
CATGGCGCTGATGCCGACCTATGCCACCATCGGCGTGGCGGCCCCGATCCTGCTCATCATCCTGCGCATGCTGCAGGGCG
CCGCCATCGGTGGTGAAGTACCGGGAGCCTGGACATTTGTCGCCGAACATGTGCCCTTCCGCCGCGTTGGCCTCGCCTGC
GGTTTCCTCACCTCCGGGCTTTCGTTCGGCATCATGCTGGGGTCGCTGATCGCCTTTGCGATCAACTCGCTCTTCACACC
GGAAGACGTGGCCGGCTACGCCTGGCGCATCCCGTTCCTGCTTGGCGGCGTTTTCGGCCTCATCGCCGTCTATCTGCGCC
GTTGGCTGGAAGAGACGCCGATCTACATGGAGATGAAGAAGTCGAAGTCCCTCACGGACAAGCTGCCGCTTGGCCTCGTG
CTGAAGCATCACAGGCGTGGCGTGATCATTTCCGCCCTGCTGACCTGGGTTCTGTCAGCGGCCATCGTCGTCACCACGCT
GATGACGGCCACCTTCCTGCAGAAGCTCTACGGTTACACCCCGACGCAGGCCTTAGCCGGTACCAGCTTCGGCACGCTGT
TCCTGATCTTCGGCGTCATCATCGCCGGAGCGCTGATCGACCGCGTCGGCTCCGGCATCTTTTTCATGGGCGCCAGCATC
TTCTTCGGCATCGCCACCTTCACCTTCTACAGCTATGCCGGCACCTCGCTCGAAACCATGTTCGTGCTTTATGGCGTGAT
GGGCCTTTCGGTGGGCATGGCCGGCGCCGTGCCCTATGTCATGGTGCGGGCCTTCCCGGCATCGGTCCGCTTCTCCGGCC
TCTCCTTCGCCTACAACGTCTCTTATGCCGTCTTCGGCGGGCTGACACCGATTGGCGTAACGACGGCGCTTGCCATCAAT
CCGATGGCCCACGCCTGGTATCTGGTCTTCATCGCCTTCCTCGCCTTCGCGATCGGCCTCTACCTCCATCTGCGCGGCAG
CGAGGTAGAAAGCCATGTCGGCATTGAGGAGCTGGCGGCATTGCGGTCGTAA

Upstream 100 bases:

>100_bases
GTCGAAATACCTGGACGATTGAGGATCGATAAGGCTTGAAATCCCGCCGCGCTTGCCGCATTCCCATAAGTAATACGTAA
TTTAGAAAAGAAGCAGGCTT

Downstream 100 bases:

>100_bases
GCTCAACAGAAAACACAAAAAAAGCGCGGGCAAGACCCGCGCTTTTTTATTTGTCCCGGCGATGCGTGAAACATCACGCA
GCGCTGTCGATTTTCTCCTC

Product: MFS permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSPTTTPAAARPLNSQDFRTLGLSALGGALEFYDFIIFVFFATVIGHLFFPPEMPDWLVMIQTFGIFAAGYLVRPLGGIV
LAHYGDRYGRKRVFAFSILLMALSTLGMALMPTYATIGVAAPILLIILRMLQGAAIGGEVPGAWTFVAEHVPFRRVGLAC
GFLTSGLSFGIMLGSLIAFAINSLFTPEDVAGYAWRIPFLLGGVFGLIAVYLRRWLEETPIYMEMKKSKSLTDKLPLGLV
LKHHRRGVIISALLTWVLSAAIVVTTLMTATFLQKLYGYTPTQALAGTSFGTLFLIFGVIIAGALIDRVGSGIFFMGASI
FFGIATFTFYSYAGTSLETMFVLYGVMGLSVGMAGAVPYVMVRAFPASVRFSGLSFAYNVSYAVFGGLTPIGVTTALAIN
PMAHAWYLVFIAFLAFAIGLYLHLRGSEVESHVGIEELAALRS

Sequences:

>Translated_443_residues
MSPTTTPAAARPLNSQDFRTLGLSALGGALEFYDFIIFVFFATVIGHLFFPPEMPDWLVMIQTFGIFAAGYLVRPLGGIV
LAHYGDRYGRKRVFAFSILLMALSTLGMALMPTYATIGVAAPILLIILRMLQGAAIGGEVPGAWTFVAEHVPFRRVGLAC
GFLTSGLSFGIMLGSLIAFAINSLFTPEDVAGYAWRIPFLLGGVFGLIAVYLRRWLEETPIYMEMKKSKSLTDKLPLGLV
LKHHRRGVIISALLTWVLSAAIVVTTLMTATFLQKLYGYTPTQALAGTSFGTLFLIFGVIIAGALIDRVGSGIFFMGASI
FFGIATFTFYSYAGTSLETMFVLYGVMGLSVGMAGAVPYVMVRAFPASVRFSGLSFAYNVSYAVFGGLTPIGVTTALAIN
PMAHAWYLVFIAFLAFAIGLYLHLRGSEVESHVGIEELAALRS
>Mature_442_residues
SPTTTPAAARPLNSQDFRTLGLSALGGALEFYDFIIFVFFATVIGHLFFPPEMPDWLVMIQTFGIFAAGYLVRPLGGIVL
AHYGDRYGRKRVFAFSILLMALSTLGMALMPTYATIGVAAPILLIILRMLQGAAIGGEVPGAWTFVAEHVPFRRVGLACG
FLTSGLSFGIMLGSLIAFAINSLFTPEDVAGYAWRIPFLLGGVFGLIAVYLRRWLEETPIYMEMKKSKSLTDKLPLGLVL
KHHRRGVIISALLTWVLSAAIVVTTLMTATFLQKLYGYTPTQALAGTSFGTLFLIFGVIIAGALIDRVGSGIFFMGASIF
FGIATFTFYSYAGTSLETMFVLYGVMGLSVGMAGAVPYVMVRAFPASVRFSGLSFAYNVSYAVFGGLTPIGVTTALAINP
MAHAWYLVFIAFLAFAIGLYLHLRGSEVESHVGIEELAALRS

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=31.1475409836066, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788292, Length=404, Percent_Identity=28.2178217821782, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=31.5245478036176, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788942, Length=452, Percent_Identity=28.7610619469027, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082231, Length=434, Percent_Identity=25.3456221198157, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=25.1908396946565, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47813; Mature: 47682

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTTTPAAARPLNSQDFRTLGLSALGGALEFYDFIIFVFFATVIGHLFFPPEMPDWLVM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IQTFGIFAAGYLVRPLGGIVLAHYGDRYGRKRVFAFSILLMALSTLGMALMPTYATIGVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APILLIILRMLQGAAIGGEVPGAWTFVAEHVPFRRVGLACGFLTSGLSFGIMLGSLIAFA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INSLFTPEDVAGYAWRIPFLLGGVFGLIAVYLRRWLEETPIYMEMKKSKSLTDKLPLGLV
HHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCHHHH
LKHHRRGVIISALLTWVLSAAIVVTTLMTATFLQKLYGYTPTQALAGTSFGTLFLIFGVI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
IAGALIDRVGSGIFFMGASIFFGIATFTFYSYAGTSLETMFVLYGVMGLSVGMAGAVPYV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MVRAFPASVRFSGLSFAYNVSYAVFGGLTPIGVTTALAINPMAHAWYLVFIAFLAFAIGL
HHHHCCCHHEECCCEEEEECHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
YLHLRGSEVESHVGIEELAALRS
HHEECCCCHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SPTTTPAAARPLNSQDFRTLGLSALGGALEFYDFIIFVFFATVIGHLFFPPEMPDWLVM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IQTFGIFAAGYLVRPLGGIVLAHYGDRYGRKRVFAFSILLMALSTLGMALMPTYATIGVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APILLIILRMLQGAAIGGEVPGAWTFVAEHVPFRRVGLACGFLTSGLSFGIMLGSLIAFA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INSLFTPEDVAGYAWRIPFLLGGVFGLIAVYLRRWLEETPIYMEMKKSKSLTDKLPLGLV
HHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCHHHH
LKHHRRGVIISALLTWVLSAAIVVTTLMTATFLQKLYGYTPTQALAGTSFGTLFLIFGVI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
IAGALIDRVGSGIFFMGASIFFGIATFTFYSYAGTSLETMFVLYGVMGLSVGMAGAVPYV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MVRAFPASVRFSGLSFAYNVSYAVFGGLTPIGVTTALAINPMAHAWYLVFIAFLAFAIGL
HHHHCCCHHEECCCEEEEECHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
YLHLRGSEVESHVGIEELAALRS
HHEECCCCHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA