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The map label for this gene is mltB [H]

Identifier: 15889403

GI number: 15889403

Start: 2085717

End: 2086943

Strand: Direct

Name: mltB [H]

Synonym: Atu2122

Alternate gene names: 15889403

Gene position: 2085717-2086943 (Clockwise)

Preceding gene: 159185057

Following gene: 159185060

Centisome position: 73.4

GC content: 61.53

Gene sequence:

>1227_bases
ATGCCCACCTTCCGGTCGCGCAGCATCGTTTTTGCCGCCGCTTTGTCGCTTCTCTCCGCCGGCTCCGCCCTTGCCACGCC
GCCTGTGGCTTCGCCTGACGATCCAAAGCAGGTTGCCTGTGGTGGTGATCTCGGCGCATTTCTCGAGGGCGTGAAGGCCG
AGGCCGTTGCCAAGGGCGTTCCAGCCGCTGCCATCGAAAAGGCGCTGGCCGGCGCTCAGATTGACCCGAAAGTGCTTTCG
CGTGACCGCGCCCAGGGCGTCTTCCGCCAGACCTTCCTGGAATTCTCCCAGCGCACCGTCAGCCAGGCGCGTCTCGATAT
CGGCCGCAAGAAGCTGCAGGAACTCTCCTCCACCTTCGCCCGCGCCGAAAATGAGTTCGGTGTACCGGCCGGCGTCATCG
CCGCCTTCTGGGCCATGGAAACCGATTTCGGCGCCGTTCAGGGCGATTTCAACACCCGCAACGCACTGGTGACCCTGTCG
CATGATTGCCGCCGCCCGGAACTTTTCCGCCCGCAGCTGCTGGCGCTGATCGAAATGGTCCAGCACGGCGATCTCGACCC
AGCCACCAATACGGGCGCATGGGCCGGCGAAATCGGCCAGGTGCAGATGCTGCCCAAGGACATCATCGCCTTTGGTGTCG
ATGGTGATGGCAACGGTCATATCAACGTCAAGGACAGCGCCCCGGATGCGATCCTGACAGCCGCGAAATTTATCCAGCAC
CTCGGCTTCAAGAAGGGCGAACCCTGGATGCAGGAAGTTTCCGTACCGGAAAACCTGCCCTGGGAAAAATCCGGCCTTGG
CGGGTCCCTGACCGCCGGCGACTGGTTTGCGCTTGGCGTGACCCCACGCGACGGCAATAAAAATTTTGCCTCCCTACCCG
CGGCCCTTATCATGCCGCAGGGCCGCAAGGGACCGACCTTCATTACCTATCCCAACTTCAATATCTATCTGGAATGGAAC
CAGTCGTTTATCTACACGACCTCGGCCGCCTATTTCGCCAACCGCCTGATGGGCGCGCCGGTCTATAACAAGGGCAATCC
CGATCCGGGCTTCGATGATGCCACGATGAAAGAGTTGCAGACCAAGCTTGAAGCGCGTGGCCACGATGTCGGCAAGGTGG
ATGGAATTCTCGGTTCCGGCACCCGCGTCGCTATCCAGAAGGAACAGCAGCGCCTCGGAATGCCCGCAGACGGATGGCCG
ACCCAGGCACTTCTCCAGGCGCTTTAA

Upstream 100 bases:

>100_bases
GAAAAATCGGCGTTGTTCTGCAACGCCAGATCGTCCGGTCCGTTTTTTTGTCGCAAACATATGGGTAAAGCGGGCCAACA
ACCTATCGAGGAAATTCCCC

Downstream 100 bases:

>100_bases
CCTGGGTTAATCGCACTACCGGGCCTTTAATCTATCGGCCGGAATCAAGGCGCAAACTGTTAATCTCGACCGGGTGACTG
GGGATAACACCCCGGTCATT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPTFRSRSIVFAAALSLLSAGSALATPPVASPDDPKQVACGGDLGAFLEGVKAEAVAKGVPAAAIEKALAGAQIDPKVLS
RDRAQGVFRQTFLEFSQRTVSQARLDIGRKKLQELSSTFARAENEFGVPAGVIAAFWAMETDFGAVQGDFNTRNALVTLS
HDCRRPELFRPQLLALIEMVQHGDLDPATNTGAWAGEIGQVQMLPKDIIAFGVDGDGNGHINVKDSAPDAILTAAKFIQH
LGFKKGEPWMQEVSVPENLPWEKSGLGGSLTAGDWFALGVTPRDGNKNFASLPAALIMPQGRKGPTFITYPNFNIYLEWN
QSFIYTTSAAYFANRLMGAPVYNKGNPDPGFDDATMKELQTKLEARGHDVGKVDGILGSGTRVAIQKEQQRLGMPADGWP
TQALLQAL

Sequences:

>Translated_408_residues
MPTFRSRSIVFAAALSLLSAGSALATPPVASPDDPKQVACGGDLGAFLEGVKAEAVAKGVPAAAIEKALAGAQIDPKVLS
RDRAQGVFRQTFLEFSQRTVSQARLDIGRKKLQELSSTFARAENEFGVPAGVIAAFWAMETDFGAVQGDFNTRNALVTLS
HDCRRPELFRPQLLALIEMVQHGDLDPATNTGAWAGEIGQVQMLPKDIIAFGVDGDGNGHINVKDSAPDAILTAAKFIQH
LGFKKGEPWMQEVSVPENLPWEKSGLGGSLTAGDWFALGVTPRDGNKNFASLPAALIMPQGRKGPTFITYPNFNIYLEWN
QSFIYTTSAAYFANRLMGAPVYNKGNPDPGFDDATMKELQTKLEARGHDVGKVDGILGSGTRVAIQKEQQRLGMPADGWP
TQALLQAL
>Mature_407_residues
PTFRSRSIVFAAALSLLSAGSALATPPVASPDDPKQVACGGDLGAFLEGVKAEAVAKGVPAAAIEKALAGAQIDPKVLSR
DRAQGVFRQTFLEFSQRTVSQARLDIGRKKLQELSSTFARAENEFGVPAGVIAAFWAMETDFGAVQGDFNTRNALVTLSH
DCRRPELFRPQLLALIEMVQHGDLDPATNTGAWAGEIGQVQMLPKDIIAFGVDGDGNGHINVKDSAPDAILTAAKFIQHL
GFKKGEPWMQEVSVPENLPWEKSGLGGSLTAGDWFALGVTPRDGNKNFASLPAALIMPQGRKGPTFITYPNFNIYLEWNQ
SFIYTTSAAYFANRLMGAPVYNKGNPDPGFDDATMKELQTKLEARGHDVGKVDGILGSGTRVAIQKEQQRLGMPADGWPT
QALLQAL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=243, Percent_Identity=28.3950617283951, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43753; Mature: 43622

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFRSRSIVFAAALSLLSAGSALATPPVASPDDPKQVACGGDLGAFLEGVKAEAVAKGV
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCC
PAAAIEKALAGAQIDPKVLSRDRAQGVFRQTFLEFSQRTVSQARLDIGRKKLQELSSTFA
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAENEFGVPAGVIAAFWAMETDFGAVQGDFNTRNALVTLSHDCRRPELFRPQLLALIEMV
HCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
QHGDLDPATNTGAWAGEIGQVQMLPKDIIAFGVDGDGNGHINVKDSAPDAILTAAKFIQH
HCCCCCCCCCCCCCCCCCCEEEECCHHHEEEECCCCCCCEEEECCCCCHHHHHHHHHHHH
LGFKKGEPWMQEVSVPENLPWEKSGLGGSLTAGDWFALGVTPRDGNKNFASLPAALIMPQ
HCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCHHEECCC
GRKGPTFITYPNFNIYLEWNQSFIYTTSAAYFANRLMGAPVYNKGNPDPGFDDATMKELQ
CCCCCEEEECCCEEEEEEECCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
TKLEARGHDVGKVDGILGSGTRVAIQKEQQRLGMPADGWPTQALLQAL
HHHHHCCCCCCHHCCEECCCCEEEEEEHHHHCCCCCCCCCHHHHHHHC
>Mature Secondary Structure 
PTFRSRSIVFAAALSLLSAGSALATPPVASPDDPKQVACGGDLGAFLEGVKAEAVAKGV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCC
PAAAIEKALAGAQIDPKVLSRDRAQGVFRQTFLEFSQRTVSQARLDIGRKKLQELSSTFA
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAENEFGVPAGVIAAFWAMETDFGAVQGDFNTRNALVTLSHDCRRPELFRPQLLALIEMV
HCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
QHGDLDPATNTGAWAGEIGQVQMLPKDIIAFGVDGDGNGHINVKDSAPDAILTAAKFIQH
HCCCCCCCCCCCCCCCCCCEEEECCHHHEEEECCCCCCCEEEECCCCCHHHHHHHHHHHH
LGFKKGEPWMQEVSVPENLPWEKSGLGGSLTAGDWFALGVTPRDGNKNFASLPAALIMPQ
HCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCHHEECCC
GRKGPTFITYPNFNIYLEWNQSFIYTTSAAYFANRLMGAPVYNKGNPDPGFDDATMKELQ
CCCCCEEEECCCEEEEEEECCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
TKLEARGHDVGKVDGILGSGTRVAIQKEQQRLGMPADGWPTQALLQAL
HHHHHCCCCCCHHCCEECCCCEEEEEEHHHHCCCCCCCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]