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The map label for this gene is amiD [H]

Identifier: 15889395

GI number: 15889395

Start: 2077310

End: 2078089

Strand: Reverse

Name: amiD [H]

Synonym: Atu2113

Alternate gene names: 15889395

Gene position: 2078089-2077310 (Counterclockwise)

Preceding gene: 159185053

Following gene: 159185052

Centisome position: 73.13

GC content: 61.41

Gene sequence:

>780_bases
ATGAAAGAATGTCTGCCGGATTTTGCCCCGGATTTCGGGGGCGCAGCCGTTGCGCCTTCGCCTAACCATGGCGAAAGACT
TGGCGTGGCCGGCCCCGATATCATTCTTCTCCACTATACCGGCATGACGACTGCGGATGGTGCCCTTTCGTGGCTCCGCA
ATCCGGAAAGCCAGGTTTCCAGCCACTATTTCGTCTTCGAGGATGGGCGTGTCATCCAGCTCGTGCCGGAAAGCCGCCGT
GCCTGGCATGCGGGAAAAAGCTCCTGGGCAGGGGATGAGGACATCAATTCCCGCTCCATCGGCATAGAAATCGCCAATCA
GGGTCACCCCGGCGGACTGCCGGAGTTTCCCGAGGCGCAGGTGGCAGCCGTCATCGAATTGTGTCGCGATTGCGGCCGGC
GCTGGTCTATCGCCCCGGAAAGGGTGCTTGCCCATTCGGATGTCGCGCCGATCCGCAAGGTCGATCCGGGCGAAAAATTC
CCGTGGGACATCCTGTCGCAGCACGGCGTCGGCCACTGGGTGGAGCCCGCGCCGATCCGCGGCGGACGCTTTTTCCAGCG
CGGCGACCATGGACAGCCGGTCGAAGCACTGCAATCGATGCTGTCCATCTATGGATATGGCGCTGAAATTACAGGCGCTT
ATTGCGAAAAAACGGAAGGGGCCGTGGCTGCCTTCCAGCGACATTTCCGCCCCGCTCTCGTGGATGGCATCGCGGATTTT
TCCACCATCGACACGCTGCACCGGCTGATCTCCAGCCTGCCGAGATTTTCGGCCGCCTGA

Upstream 100 bases:

>100_bases
TTGCGCGCGGCGTGCCGATGGAATTGCATGCTGCTGCAAATGAGCGTATGGCGGCTCTCAACGCCGCCTATGCGGCCATC
GAGAAAGAACGCCGCGTCGC

Downstream 100 bases:

>100_bases
GCGCGGATTCTGGAAAAGCATTCCCGTTTTTCCAGCGCCTCCAAGTCGCTTTTTTGTTGTGCCACAGTTAATCCGCATTG
AGGAAGTCTAAGAACGGCAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKECLPDFAPDFGGAAVAPSPNHGERLGVAGPDIILLHYTGMTTADGALSWLRNPESQVSSHYFVFEDGRVIQLVPESRR
AWHAGKSSWAGDEDINSRSIGIEIANQGHPGGLPEFPEAQVAAVIELCRDCGRRWSIAPERVLAHSDVAPIRKVDPGEKF
PWDILSQHGVGHWVEPAPIRGGRFFQRGDHGQPVEALQSMLSIYGYGAEITGAYCEKTEGAVAAFQRHFRPALVDGIADF
STIDTLHRLISSLPRFSAA

Sequences:

>Translated_259_residues
MKECLPDFAPDFGGAAVAPSPNHGERLGVAGPDIILLHYTGMTTADGALSWLRNPESQVSSHYFVFEDGRVIQLVPESRR
AWHAGKSSWAGDEDINSRSIGIEIANQGHPGGLPEFPEAQVAAVIELCRDCGRRWSIAPERVLAHSDVAPIRKVDPGEKF
PWDILSQHGVGHWVEPAPIRGGRFFQRGDHGQPVEALQSMLSIYGYGAEITGAYCEKTEGAVAAFQRHFRPALVDGIADF
STIDTLHRLISSLPRFSAA
>Mature_259_residues
MKECLPDFAPDFGGAAVAPSPNHGERLGVAGPDIILLHYTGMTTADGALSWLRNPESQVSSHYFVFEDGRVIQLVPESRR
AWHAGKSSWAGDEDINSRSIGIEIANQGHPGGLPEFPEAQVAAVIELCRDCGRRWSIAPERVLAHSDVAPIRKVDPGEKF
PWDILSQHGVGHWVEPAPIRGGRFFQRGDHGQPVEALQSMLSIYGYGAEITGAYCEKTEGAVAAFQRHFRPALVDGIADF
STIDTLHRLISSLPRFSAA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=223, Percent_Identity=39.0134529147982, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1786300, Length=166, Percent_Identity=30.1204819277108, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28187; Mature: 28187

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECLPDFAPDFGGAAVAPSPNHGERLGVAGPDIILLHYTGMTTADGALSWLRNPESQVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHH
SHYFVFEDGRVIQLVPESRRAWHAGKSSWAGDEDINSRSIGIEIANQGHPGGLPEFPEAQ
CEEEEEECCCEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
VAAVIELCRDCGRRWSIAPERVLAHSDVAPIRKVDPGEKFPWDILSQHGVGHWVEPAPIR
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
GGRFFQRGDHGQPVEALQSMLSIYGYGAEITGAYCEKTEGAVAAFQRHFRPALVDGIADF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHH
STIDTLHRLISSLPRFSAA
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKECLPDFAPDFGGAAVAPSPNHGERLGVAGPDIILLHYTGMTTADGALSWLRNPESQVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHH
SHYFVFEDGRVIQLVPESRRAWHAGKSSWAGDEDINSRSIGIEIANQGHPGGLPEFPEAQ
CEEEEEECCCEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
VAAVIELCRDCGRRWSIAPERVLAHSDVAPIRKVDPGEKFPWDILSQHGVGHWVEPAPIR
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
GGRFFQRGDHGQPVEALQSMLSIYGYGAEITGAYCEKTEGAVAAFQRHFRPALVDGIADF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHH
STIDTLHRLISSLPRFSAA
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]