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The map label for this gene is murF [H]

Identifier: 15889382

GI number: 15889382

Start: 2062279

End: 2063712

Strand: Reverse

Name: murF [H]

Synonym: Atu2098

Alternate gene names: 15889382

Gene position: 2063712-2062279 (Counterclockwise)

Preceding gene: 159185046

Following gene: 15889381

Centisome position: 72.63

GC content: 62.48

Gene sequence:

>1434_bases
TTGAGCTGGTTATGGACAGTCGCCGACATGATCGCCATCACGGCAGGCCGCCCGGTGGGCAACCTGCCGACCGGTATCAC
CGGCATTTCCATCGACAGCCGGACGGTCAAACAGGGTGAAGCCTTCTTCGCGATCAAGGGTGATCGGGTAGACGGCCATG
ACTATACCAGCTTTGCCGTTGCCAACGGCGCCAGCCTTCTGGTGGTTGCCGAAGCCAAGCTGCCGGCGCTTGGTCGCCTA
ACCGTGCCGATGATCGTGGTCGAGGACGTACTTGCAGCCCTTGGCAAGCTTGCCCTTGCGGCGCGCGAAAGAACCTCGGC
GCGCATCATCGCGGTGACCGGTTCGGTGGGTAAGACAACGACCAAGGAGATGCTGCGGCAGGCTCTGGCCCCTTCGGGGC
GGGTGCATGCGGCGGTGGCGTCCTTCAACAACCATTGGGGCGTGCCGCTGACGCTCGCACGCATGCCTGCCGATACGGAA
TTCGGCGTGTTCGAAATCGGCATGAACCATTCCGGCGAAATCCGGCCGCTGGTAAAGATGGTCCGCCCGCATGTGGCGAT
CATCACCACCATCGCGCCCGCCCATCTCGGCAATTTCAAGAATATCGAAGAGATCGCTGCCGCGAAGGCCGAAATTTTCG
AAGGCGTGGAAGAGGGCGGCTCCGTCATCCTCAACCGCGACAACGCCCAGTTCGAGCAGCTTGAAGAGGCGGCGCAGGAG
GCGGGTATCGAACATATCCTCACCTTCGGCCAGCATGCCAAGGCCGATTTCCGCCTTGCGGATTTCGAGAGCACGACGAC
GGGTTCGACGATCTGGGCGATCCTCAACGGCGAAACCAGCGAGCTGCACATCAACATTCCCGGCCGGCACATCGCCGACA
ATGCGATGGCGGTCCTTGGTGCCGTTGCCGTCACCGGTGCCAATCTCGACCGTGCGCTTGAGGCGCTCGGAACGCTCGAG
GCGGTGAAGGGACGTGGCCAGCGCCACCACCTGACGATCAATGGCGGATCCTTCCTGCTGATCGATGAAAGCTACAATGC
CAATCCGGCCTCCATGCGTGCGGCGATTGCCGTGCTGGCGGAAACGGAAACGCAGGGTCACGGCCGGCGCGTCGCCGTTC
TCGGCGATATGCTGGAAATGGGCGAGTTTTCGGCGCAGTTGCACGAAGAGCTGGCCGGGCCGCTGCTCGCTGCCGGTATC
GAACATGTCTGGCTCGCGGGCGAGGCCATGGCCGCGCTTCGCGACGCGCTGCCTGACAGCGTCACCGTCATCTGGTTCCC
GACGACGGCGGAACTCACCGATTTTGCCCTGCAATGGGTGCAACCGGGAGACGCGCTGATGGTAAAATCGTCACTTGGGC
TTGGTTTCGGCAAGATTGTCGCCGCCCTGCTTGACAAGTATCCGGCATTCCCCGAGACGGAACGCCAAGTCTGA

Upstream 100 bases:

>100_bases
AGGGGCACGAAGAAGGGCAGATCGTCGGCTCCGTGACGCTGCCGTTTTCCGATCATGAACAGGTGCGCTCTGCACTGGCA
GAGCTGGAAGGATCGAAGAT

Downstream 100 bases:

>100_bases
AATAGCCTTTTGAAAGGGCAATTATGCTGATCTGGCTCGTCGAACTGTCGGACAAAGTTCAACTGTTCAACCTCTTCCGG
TACATCACCTTCCGGGCGGG

Product: UDP-MurNAc-pentapeptide synthetase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSWLWTVADMIAITAGRPVGNLPTGITGISIDSRTVKQGEAFFAIKGDRVDGHDYTSFAVANGASLLVVAEAKLPALGRL
TVPMIVVEDVLAALGKLALAARERTSARIIAVTGSVGKTTTKEMLRQALAPSGRVHAAVASFNNHWGVPLTLARMPADTE
FGVFEIGMNHSGEIRPLVKMVRPHVAIITTIAPAHLGNFKNIEEIAAAKAEIFEGVEEGGSVILNRDNAQFEQLEEAAQE
AGIEHILTFGQHAKADFRLADFESTTTGSTIWAILNGETSELHINIPGRHIADNAMAVLGAVAVTGANLDRALEALGTLE
AVKGRGQRHHLTINGGSFLLIDESYNANPASMRAAIAVLAETETQGHGRRVAVLGDMLEMGEFSAQLHEELAGPLLAAGI
EHVWLAGEAMAALRDALPDSVTVIWFPTTAELTDFALQWVQPGDALMVKSSLGLGFGKIVAALLDKYPAFPETERQV

Sequences:

>Translated_477_residues
MSWLWTVADMIAITAGRPVGNLPTGITGISIDSRTVKQGEAFFAIKGDRVDGHDYTSFAVANGASLLVVAEAKLPALGRL
TVPMIVVEDVLAALGKLALAARERTSARIIAVTGSVGKTTTKEMLRQALAPSGRVHAAVASFNNHWGVPLTLARMPADTE
FGVFEIGMNHSGEIRPLVKMVRPHVAIITTIAPAHLGNFKNIEEIAAAKAEIFEGVEEGGSVILNRDNAQFEQLEEAAQE
AGIEHILTFGQHAKADFRLADFESTTTGSTIWAILNGETSELHINIPGRHIADNAMAVLGAVAVTGANLDRALEALGTLE
AVKGRGQRHHLTINGGSFLLIDESYNANPASMRAAIAVLAETETQGHGRRVAVLGDMLEMGEFSAQLHEELAGPLLAAGI
EHVWLAGEAMAALRDALPDSVTVIWFPTTAELTDFALQWVQPGDALMVKSSLGLGFGKIVAALLDKYPAFPETERQV
>Mature_476_residues
SWLWTVADMIAITAGRPVGNLPTGITGISIDSRTVKQGEAFFAIKGDRVDGHDYTSFAVANGASLLVVAEAKLPALGRLT
VPMIVVEDVLAALGKLALAARERTSARIIAVTGSVGKTTTKEMLRQALAPSGRVHAAVASFNNHWGVPLTLARMPADTEF
GVFEIGMNHSGEIRPLVKMVRPHVAIITTIAPAHLGNFKNIEEIAAAKAEIFEGVEEGGSVILNRDNAQFEQLEEAAQEA
GIEHILTFGQHAKADFRLADFESTTTGSTIWAILNGETSELHINIPGRHIADNAMAVLGAVAVTGANLDRALEALGTLEA
VKGRGQRHHLTINGGSFLLIDESYNANPASMRAAIAVLAETETQGHGRRVAVLGDMLEMGEFSAQLHEELAGPLLAAGIE
HVWLAGEAMAALRDALPDSVTVIWFPTTAELTDFALQWVQPGDALMVKSSLGLGFGKIVAALLDKYPAFPETERQV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=392, Percent_Identity=35.2040816326531, Blast_Score=194, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50686; Mature: 50554

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWLWTVADMIAITAGRPVGNLPTGITGISIDSRTVKQGEAFFAIKGDRVDGHDYTSFAV
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEE
ANGASLLVVAEAKLPALGRLTVPMIVVEDVLAALGKLALAARERTSARIIAVTGSVGKTT
ECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
TKEMLRQALAPSGRVHAAVASFNNHWGVPLTLARMPADTEFGVFEIGMNHSGEIRPLVKM
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHHH
VRPHVAIITTIAPAHLGNFKNIEEIAAAKAEIFEGVEEGGSVILNRDNAQFEQLEEAAQE
HCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
AGIEHILTFGQHAKADFRLADFESTTTGSTIWAILNGETSELHINIPGRHIADNAMAVLG
CCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
AVAVTGANLDRALEALGTLEAVKGRGQRHHLTINGGSFLLIDESYNANPASMRAAIAVLA
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHEEE
ETETQGHGRRVAVLGDMLEMGEFSAQLHEELAGPLLAAGIEHVWLAGEAMAALRDALPDS
ECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHCHHHEEECHHHHHHHHHCCCCC
VTVIWFPTTAELTDFALQWVQPGDALMVKSSLGLGFGKIVAALLDKYPAFPETERQV
EEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SWLWTVADMIAITAGRPVGNLPTGITGISIDSRTVKQGEAFFAIKGDRVDGHDYTSFAV
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEE
ANGASLLVVAEAKLPALGRLTVPMIVVEDVLAALGKLALAARERTSARIIAVTGSVGKTT
ECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
TKEMLRQALAPSGRVHAAVASFNNHWGVPLTLARMPADTEFGVFEIGMNHSGEIRPLVKM
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHHH
VRPHVAIITTIAPAHLGNFKNIEEIAAAKAEIFEGVEEGGSVILNRDNAQFEQLEEAAQE
HCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
AGIEHILTFGQHAKADFRLADFESTTTGSTIWAILNGETSELHINIPGRHIADNAMAVLG
CCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
AVAVTGANLDRALEALGTLEAVKGRGQRHHLTINGGSFLLIDESYNANPASMRAAIAVLA
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHEEE
ETETQGHGRRVAVLGDMLEMGEFSAQLHEELAGPLLAAGIEHVWLAGEAMAALRDALPDS
ECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHCHHHEEECHHHHHHHHHCCCCC
VTVIWFPTTAELTDFALQWVQPGDALMVKSSLGLGFGKIVAALLDKYPAFPETERQV
EEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]