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The map label for this gene is murD

Identifier: 15889380

GI number: 15889380

Start: 2059742

End: 2061151

Strand: Reverse

Name: murD

Synonym: Atu2096

Alternate gene names: 15889380

Gene position: 2061151-2059742 (Counterclockwise)

Preceding gene: 15889381

Following gene: 15889379

Centisome position: 72.54

GC content: 60.99

Gene sequence:

>1410_bases
ATGATCCCGGTCACGTCGTTTAGGGGTAAGAAAGTCGCTCTCTTCGGGCTCGGCGGTTCCGGTCTCGTCACCGCCCGCGC
GCTGGTTGCCGGTGGTGCTGCTGTCGTTGCTTTTGACGACAATCCTGACAGCGTCGCGAAGGCGCAGGCCGAGGGTATTG
CGACTGCGGATCTCCACACGATTGACTGGTCTTCGTTTTCCTCGTTTGTGCTGGCTCCGGGAGTCCCGTTAACGCATCCG
AAGCCGCATTGGTCGGTGGACCTTGCGAAGGCGGCGGGCGTCGAAGTGATCGGCGATATCGAGCTTTTCATCCGTGAGCG
GCGGGTGCATGCGCCGGATTGCCCTTTCATTGCGATTACCGGCACCAACGGCAAGTCGACGACGACAGCGTTGATCGCGC
ATATTCTGCAATCATCGGGCCGCGATACCCAGCTCGGCGGCAATATCGGCACGGCGGTGCTGAGCCTCGATCCGCCCAAG
GCACAACGTTTCTACGTGGTGGAATGCTCGTCCTACCAGATCGATCTGGCGCCGACGATCAATCCGACCGCCGGCATTCT
GCTCAATCTGACGCCTGACCATCTCGACCGGCACGGCACGATGCAGCATTATGCCGACGTCAAGGAACGGCTGGTGGCCG
GCAGCGGCACTGCGATTGTCGGCGTGGATGACAGTCATTCGACGCTGATTGCCGATCGCATCGAGCGCGCCGGCGTCAAG
GTGGAGCGCATCTCCAAGCGCAATGTCGTTTCCGAAGGTCTCTATGCCGAAGGCAGCCAGATACTCAGGGCGCATGGCGG
CACGTCTTCGCTGCTGGTCGATCTCGACGGTATCCAGACGCTTCGCGGCAGCCACAATGCACAGAATGCAGCGGCAGCGA
TTGCTGCCTGCCTTGCGGTCGGTGTTTCCGAAGAGGAAATTCGTGCCGGTCTCAAGTCCTTCCCCGGTCTCAAGCACCGC
ATGCAGCCCGTCGGCCGGCGTGGCAATGTCACCTTCGTTAATGATAGCAAGGCGACCAACGCCGATGCGGCAGCGCCCGC
ACTTTCGAGCTTTGACCGCATCTACTGGATCGCCGGCGGTTTGCCCAAGGCGGGCGGCATCACCAGCCTTTCGCCGCTCT
TCCCGCGCATCGCCAAGGCCTATCTGATCGGTGAGGCAGCGGCGGAATTTGCTGCGACGCTTGGCGAGGCGGTGCCTTAC
GAAATATCCGGTACGCTGGACAAGGCGGTGCAGCACGCGGCGGCGGATGCGGAAAAAGATGCGACGGCCGGCAATGTCGT
GATGTTATCCCCGGCTTGCGCAAGCTTCGATCAATATAAGAACTTTGAAATCAGAGGTGATTCGTTCGTCGCGCAGGTTG
CGGCGCTCGAGGGCATGACGATGCTCGTTCACTCTGGAAAAGGGGGCTGA

Upstream 100 bases:

>100_bases
GAAAAAGGGCTGGACGGAAAGCCAGGTGGTTATCCGTTTCTGGATCATCTCCGTCGGCCTCGCGCTGCTCGGTCTTGCCA
CCCTGAAGCTGAGGTGAGCG

Downstream 100 bases:

>100_bases
GGCCATGGTAAGCCGAGTTGATCGCGGTCCGGTCGCGGAGTGGTTCTGGACCATCGATCGTTTCTTTCTGGCGGCGTTTG
TCGCGCTGATGGGTATCGGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MIPVTSFRGKKVALFGLGGSGLVTARALVAGGAAVVAFDDNPDSVAKAQAEGIATADLHTIDWSSFSSFVLAPGVPLTHP
KPHWSVDLAKAAGVEVIGDIELFIRERRVHAPDCPFIAITGTNGKSTTTALIAHILQSSGRDTQLGGNIGTAVLSLDPPK
AQRFYVVECSSYQIDLAPTINPTAGILLNLTPDHLDRHGTMQHYADVKERLVAGSGTAIVGVDDSHSTLIADRIERAGVK
VERISKRNVVSEGLYAEGSQILRAHGGTSSLLVDLDGIQTLRGSHNAQNAAAAIAACLAVGVSEEEIRAGLKSFPGLKHR
MQPVGRRGNVTFVNDSKATNADAAAPALSSFDRIYWIAGGLPKAGGITSLSPLFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLDKAVQHAAADAEKDATAGNVVMLSPACASFDQYKNFEIRGDSFVAQVAALEGMTMLVHSGKGG

Sequences:

>Translated_469_residues
MIPVTSFRGKKVALFGLGGSGLVTARALVAGGAAVVAFDDNPDSVAKAQAEGIATADLHTIDWSSFSSFVLAPGVPLTHP
KPHWSVDLAKAAGVEVIGDIELFIRERRVHAPDCPFIAITGTNGKSTTTALIAHILQSSGRDTQLGGNIGTAVLSLDPPK
AQRFYVVECSSYQIDLAPTINPTAGILLNLTPDHLDRHGTMQHYADVKERLVAGSGTAIVGVDDSHSTLIADRIERAGVK
VERISKRNVVSEGLYAEGSQILRAHGGTSSLLVDLDGIQTLRGSHNAQNAAAAIAACLAVGVSEEEIRAGLKSFPGLKHR
MQPVGRRGNVTFVNDSKATNADAAAPALSSFDRIYWIAGGLPKAGGITSLSPLFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLDKAVQHAAADAEKDATAGNVVMLSPACASFDQYKNFEIRGDSFVAQVAALEGMTMLVHSGKGG
>Mature_469_residues
MIPVTSFRGKKVALFGLGGSGLVTARALVAGGAAVVAFDDNPDSVAKAQAEGIATADLHTIDWSSFSSFVLAPGVPLTHP
KPHWSVDLAKAAGVEVIGDIELFIRERRVHAPDCPFIAITGTNGKSTTTALIAHILQSSGRDTQLGGNIGTAVLSLDPPK
AQRFYVVECSSYQIDLAPTINPTAGILLNLTPDHLDRHGTMQHYADVKERLVAGSGTAIVGVDDSHSTLIADRIERAGVK
VERISKRNVVSEGLYAEGSQILRAHGGTSSLLVDLDGIQTLRGSHNAQNAAAAIAACLAVGVSEEEIRAGLKSFPGLKHR
MQPVGRRGNVTFVNDSKATNADAAAPALSSFDRIYWIAGGLPKAGGITSLSPLFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLDKAVQHAAADAEKDATAGNVVMLSPACASFDQYKNFEIRGDSFVAQVAALEGMTMLVHSGKGG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=458, Percent_Identity=32.0960698689956, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_AGRT5 (Q8UDM6)

Other databases:

- EMBL:   AE007869
- PIR:   AI2833
- PIR:   E97611
- RefSeq:   NP_355061.1
- ProteinModelPortal:   Q8UDM6
- SMR:   Q8UDM6
- STRING:   Q8UDM6
- GeneID:   1134134
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2096
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q8UDM6
- ProtClustDB:   PRK01390
- BioCyc:   ATUM176299-1:ATU2096-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48875; Mature: 48875

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTSFRGKKVALFGLGGSGLVTARALVAGGAAVVAFDDNPDSVAKAQAEGIATADLHT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEEE
IDWSSFSSFVLAPGVPLTHPKPHWSVDLAKAAGVEVIGDIELFIRERRVHAPDCPFIAIT
ECCCCCCCEEECCCCCCCCCCCCCEEEHHHHCCCEEEEHHHHHHHHCCCCCCCCCEEEEE
GTNGKSTTTALIAHILQSSGRDTQLGGNIGTAVLSLDPPKAQRFYVVECSSYQIDLAPTI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGTMQHYADVKERLVAGSGTAIVGVDDSHSTLIADRIERAGVK
CCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCC
VERISKRNVVSEGLYAEGSQILRAHGGTSSLLVDLDGIQTLRGSHNAQNAAAAIAACLAV
HHHHHHHHHHHCCCCCCCCHHEEECCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHH
GVSEEEIRAGLKSFPGLKHRMQPVGRRGNVTFVNDSKATNADAAAPALSSFDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEEECC
LPKAGGITSLSPLFPRIAKAYLIGEAAAEFAATLGEAVPYEISGTLDKAVQHAAADAEKD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCC
ATAGNVVMLSPACASFDQYKNFEIRGDSFVAQVAALEGMTMLVHSGKGG
CCCCCEEEECCCHHCHHHHCCEEECCCHHHHHHHHHCCEEEEEECCCCC
>Mature Secondary Structure
MIPVTSFRGKKVALFGLGGSGLVTARALVAGGAAVVAFDDNPDSVAKAQAEGIATADLHT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEEE
IDWSSFSSFVLAPGVPLTHPKPHWSVDLAKAAGVEVIGDIELFIRERRVHAPDCPFIAIT
ECCCCCCCEEECCCCCCCCCCCCCEEEHHHHCCCEEEEHHHHHHHHCCCCCCCCCEEEEE
GTNGKSTTTALIAHILQSSGRDTQLGGNIGTAVLSLDPPKAQRFYVVECSSYQIDLAPTI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGTMQHYADVKERLVAGSGTAIVGVDDSHSTLIADRIERAGVK
CCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCC
VERISKRNVVSEGLYAEGSQILRAHGGTSSLLVDLDGIQTLRGSHNAQNAAAAIAACLAV
HHHHHHHHHHHCCCCCCCCHHEEECCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHH
GVSEEEIRAGLKSFPGLKHRMQPVGRRGNVTFVNDSKATNADAAAPALSSFDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEEECC
LPKAGGITSLSPLFPRIAKAYLIGEAAAEFAATLGEAVPYEISGTLDKAVQHAAADAEKD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCC
ATAGNVVMLSPACASFDQYKNFEIRGDSFVAQVAALEGMTMLVHSGKGG
CCCCCEEEECCCHHCHHHHCCEEECCCHHHHHHHHHCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194