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The map label for this gene is ligA

Identifier: 15889366

GI number: 15889366

Start: 2040803

End: 2042977

Strand: Reverse

Name: ligA

Synonym: Atu2082

Alternate gene names: 15889366

Gene position: 2042977-2040803 (Counterclockwise)

Preceding gene: 15889367

Following gene: 15889365

Centisome position: 71.9

GC content: 59.91

Gene sequence:

>2175_bases
ATGTCGAAAGACCAGATTCCCGTCGAAAACCTGACCGAGCTTGAAGCCTCTTCGGAGCTGGCGTTTCTGGCAGCCGAGCT
TGCCCGTCATGATGCGCTTTACCACGGCAAGGACGCACCGGAGATTTCCGATGCCGACTATGATGCGCTGAAGCGGCGCA
ACGATGCCATCGAAGCGGCGTTTCCGGCATTGGTGCGGGCTGACAGCCCGTCGAAGAAAGTCGGCTTCACACCGTTGCCG
ACCTTCGCGCCCATCGTGCATGCCCGTCCGATGCTGTCGCTCGACAATACGTTTTCGGAAGAGGACCTGCGCGATTTCGT
CAGTTCCGTCTATCGTTTCCTCGGGCACTTGCCTGATGATTCCATCGCCTTTACCGCCGAGCCGAAGATCGACGGGCTTT
CCATGTCGATCCGCTATGAGAACCGCAAGCTCAAAACGGCGGCGACGCGCGGCGATGGTACGACCGGCGAAAACGTCACC
GCCAATATCAGGACCATCAAGGAAATTCCGAACGAACTGCCGGCCGATGCGCCCGATGTGGTGGAGGTACGCGGCGAAGT
CTATATGGCCAAGAGCGACTTCCTGGCGCTGAACGCCCAGATGGAGGCCGATGGCAAGCAGACCTATGTGAACCCGCGCA
ACACGGCATCCGGTTCTCTGCGCCAGCTCGATGCGAATGTGACGGCGAAACGCAAGCTGCGGTTCTTTGCCTACGCGCTG
GGTGAAGTCTCCAACGGCGGCCAGCCGGCGCGGATTGCCGATACGCAATATGGCATCGTCGAGAAGTTCCGGGAGTGGGG
ATTCCCGGTCAATCCGCTGATGAAGCGATTCACCTCCGCCCAGCAATTGCTTGAGCATTATAATGAAATCGGTGTCGCAC
GGCCCGATCTCGATTACGATATTGACGGCGTCGTCTATAAGGTCGACCGGCTCGATCTTCAGGAACGTCTGGGTTTCCGC
TCCCGCAGCCCGCGCTGGGCCACTGCACACAAGTTTCCGGCCGAGCAGGCCTTTACCACCGTCGAGAATATCGAAATTCA
GGTGGGACGCACCGGCGCTTTGACGCCGGTGGCGCGCCTCACGCCGATCACCGTTGGCGGCGTGGTCGTCACCAATGCGA
CGTTGCACAATGCCGATTATATCGAAGGCATCGGCAATAGCGGCGAGCGTATCCGCCCGGAGGATCATGACATTCGCATC
GGCGACACTGTCATCGTGCAGCGTGCCGGCGATGTCATTCCGCAGGTGCTGGATGTGCTTCTGGAGAAGCGCGCGGCTGG
GGCGGCGAAATATGTTTTTCCGGAGAAATGTCCGGTCTGCGGCAGCCATGCGGTGCGGGAACGCAACGAAAAGACCGGCA
AGCTCGATTCGGTCACCCGCTGCACCGGCGGTTTCGTCTGCCGGGCGCAGGCGGTGGAGCATCTGAAACATTTCGTCTCG
CGTAATGCCTTCGACATTGAAGGGCTGGGCACCAAGCAGATCGATTTCTTCTTCGAAAGCGACGATCCGGCACTTTCGAT
CAAGACCGCGCCTGACATTTTTACGCTGAAGGCGCGGCAGGAAGCGTCTCATCTGACCAAGCTCGAGAATATCGACGGCT
TCGGCAAGGTTAGCGTGAAGAAGCTGTTCGATGCGATCGATGCCCGCCGTGCGATTGATCTACACCGGCTGATTTTCGCG
CTCGGCATCCGTCACGTCGGCGAGACCACGGCGAAGCTTCTGGCGCGTTCCTATGGCACTTATGAGCATTTCGAGAAGGC
GATGAAGGCGGCGGCCGATCCGGCGAGCGATGCCTGGGCGGAACTCAACAGTATCGACGGCATCGGCGAGGTGGTGGCCC
GTGCCATCATCGAGTTTTACAAGGAGCCGCGCAATCTCGATGTTATCGACCGGCTGATACGGGAGCTTCAGCCGAAGGAG
GCCGAGAAGCCTTCAACTGAGGGCAGCCCGGTGGCAGGCAAGACTGTCGTCTTTACCGGCTCGCTGGAAAAATTCACCCG
CGATGAGGCGAAGGCGCGGGCCGAAAGCCTGGGCGCAAAGGTTGCCGGTTCCGTTTCGAAAAAGACGGATATTCTGGTGG
CAGGGCCGGGGGCGGGCTCCAAGCTTGCCAAGGCGACGGAGCTGGGTGTGCAGACCATGGACGAGGATGAGTGGCTGGCG
TTGATCGGCGGCTGA

Upstream 100 bases:

>100_bases
GCCTTTGGTGTTGTGGGTCTTGTGAGACTGGCGGCAAAACTGATTTTATTGCTGTTTCATTTCGGCTAAAAGCGTTCGGT
AATTCTGAAACGAGGCCGCT

Downstream 100 bases:

>100_bases
TACGGCGTCATCGCTGTGTTGCCTTGCACCGCTAGAGGTTTGCGCCTGAAACGGGGAACGCATCATGGATGACCGCATTG
TCGTCACCACTCAGCAGGTC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MSKDQIPVENLTELEASSELAFLAAELARHDALYHGKDAPEISDADYDALKRRNDAIEAAFPALVRADSPSKKVGFTPLP
TFAPIVHARPMLSLDNTFSEEDLRDFVSSVYRFLGHLPDDSIAFTAEPKIDGLSMSIRYENRKLKTAATRGDGTTGENVT
ANIRTIKEIPNELPADAPDVVEVRGEVYMAKSDFLALNAQMEADGKQTYVNPRNTASGSLRQLDANVTAKRKLRFFAYAL
GEVSNGGQPARIADTQYGIVEKFREWGFPVNPLMKRFTSAQQLLEHYNEIGVARPDLDYDIDGVVYKVDRLDLQERLGFR
SRSPRWATAHKFPAEQAFTTVENIEIQVGRTGALTPVARLTPITVGGVVVTNATLHNADYIEGIGNSGERIRPEDHDIRI
GDTVIVQRAGDVIPQVLDVLLEKRAAGAAKYVFPEKCPVCGSHAVRERNEKTGKLDSVTRCTGGFVCRAQAVEHLKHFVS
RNAFDIEGLGTKQIDFFFESDDPALSIKTAPDIFTLKARQEASHLTKLENIDGFGKVSVKKLFDAIDARRAIDLHRLIFA
LGIRHVGETTAKLLARSYGTYEHFEKAMKAAADPASDAWAELNSIDGIGEVVARAIIEFYKEPRNLDVIDRLIRELQPKE
AEKPSTEGSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDILVAGPGAGSKLAKATELGVQTMDEDEWLA
LIGG

Sequences:

>Translated_724_residues
MSKDQIPVENLTELEASSELAFLAAELARHDALYHGKDAPEISDADYDALKRRNDAIEAAFPALVRADSPSKKVGFTPLP
TFAPIVHARPMLSLDNTFSEEDLRDFVSSVYRFLGHLPDDSIAFTAEPKIDGLSMSIRYENRKLKTAATRGDGTTGENVT
ANIRTIKEIPNELPADAPDVVEVRGEVYMAKSDFLALNAQMEADGKQTYVNPRNTASGSLRQLDANVTAKRKLRFFAYAL
GEVSNGGQPARIADTQYGIVEKFREWGFPVNPLMKRFTSAQQLLEHYNEIGVARPDLDYDIDGVVYKVDRLDLQERLGFR
SRSPRWATAHKFPAEQAFTTVENIEIQVGRTGALTPVARLTPITVGGVVVTNATLHNADYIEGIGNSGERIRPEDHDIRI
GDTVIVQRAGDVIPQVLDVLLEKRAAGAAKYVFPEKCPVCGSHAVRERNEKTGKLDSVTRCTGGFVCRAQAVEHLKHFVS
RNAFDIEGLGTKQIDFFFESDDPALSIKTAPDIFTLKARQEASHLTKLENIDGFGKVSVKKLFDAIDARRAIDLHRLIFA
LGIRHVGETTAKLLARSYGTYEHFEKAMKAAADPASDAWAELNSIDGIGEVVARAIIEFYKEPRNLDVIDRLIRELQPKE
AEKPSTEGSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDILVAGPGAGSKLAKATELGVQTMDEDEWLA
LIGG
>Mature_723_residues
SKDQIPVENLTELEASSELAFLAAELARHDALYHGKDAPEISDADYDALKRRNDAIEAAFPALVRADSPSKKVGFTPLPT
FAPIVHARPMLSLDNTFSEEDLRDFVSSVYRFLGHLPDDSIAFTAEPKIDGLSMSIRYENRKLKTAATRGDGTTGENVTA
NIRTIKEIPNELPADAPDVVEVRGEVYMAKSDFLALNAQMEADGKQTYVNPRNTASGSLRQLDANVTAKRKLRFFAYALG
EVSNGGQPARIADTQYGIVEKFREWGFPVNPLMKRFTSAQQLLEHYNEIGVARPDLDYDIDGVVYKVDRLDLQERLGFRS
RSPRWATAHKFPAEQAFTTVENIEIQVGRTGALTPVARLTPITVGGVVVTNATLHNADYIEGIGNSGERIRPEDHDIRIG
DTVIVQRAGDVIPQVLDVLLEKRAAGAAKYVFPEKCPVCGSHAVRERNEKTGKLDSVTRCTGGFVCRAQAVEHLKHFVSR
NAFDIEGLGTKQIDFFFESDDPALSIKTAPDIFTLKARQEASHLTKLENIDGFGKVSVKKLFDAIDARRAIDLHRLIFAL
GIRHVGETTAKLLARSYGTYEHFEKAMKAAADPASDAWAELNSIDGIGEVVARAIIEFYKEPRNLDVIDRLIRELQPKEA
EKPSTEGSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDILVAGPGAGSKLAKATELGVQTMDEDEWLAL
IGG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=710, Percent_Identity=45.7746478873239, Blast_Score=577, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_AGRT5 (A9CIB3)

Other databases:

- EMBL:   AE007869
- PIR:   AC2832
- PIR:   G97609
- RefSeq:   NP_355047.1
- ProteinModelPortal:   A9CIB3
- STRING:   A9CIB3
- GeneID:   1134120
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2082
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A9CIB3
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 79393; Mature: 79261

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 129-129 BINDING 127-127 BINDING 150-150 BINDING 186-186 BINDING 307-307 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDQIPVENLTELEASSELAFLAAELARHDALYHGKDAPEISDADYDALKRRNDAIEAA
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHH
FPALVRADSPSKKVGFTPLPTFAPIVHARPMLSLDNTFSEEDLRDFVSSVYRFLGHLPDD
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SIAFTAEPKIDGLSMSIRYENRKLKTAATRGDGTTGENVTANIRTIKEIPNELPADAPDV
CEEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCH
VEVRGEVYMAKSDFLALNAQMEADGKQTYVNPRNTASGSLRQLDANVTAKRKLRFFAYAL
HEECCCEEEEECCEEEEEEEECCCCCEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
GEVSNGGQPARIADTQYGIVEKFREWGFPVNPLMKRFTSAQQLLEHYNEIGVARPDLDYD
HHCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IDGVVYKVDRLDLQERLGFRSRSPRWATAHKFPAEQAFTTVENIEIQVGRTGALTPVARL
CCCEEEEEHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHH
TPITVGGVVVTNATLHNADYIEGIGNSGERIRPEDHDIRIGDTVIVQRAGDVIPQVLDVL
CCEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHH
LEKRAAGAAKYVFPEKCPVCGSHAVRERNEKTGKLDSVTRCTGGFVCRAQAVEHLKHFVS
HHHHHCCCEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
RNAFDIEGLGTKQIDFFFESDDPALSIKTAPDIFTLKARQEASHLTKLENIDGFGKVSVK
CCCEEECCCCCCEEEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCHHHH
KLFDAIDARRAIDLHRLIFALGIRHVGETTAKLLARSYGTYEHFEKAMKAAADPASDAWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
ELNSIDGIGEVVARAIIEFYKEPRNLDVIDRLIRELQPKEAEKPSTEGSPVAGKTVVFTG
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
SLEKFTRDEAKARAESLGAKVAGSVSKKTDILVAGPGAGSKLAKATELGVQTMDEDEWLA
CHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEE
LIGG
EECC
>Mature Secondary Structure 
SKDQIPVENLTELEASSELAFLAAELARHDALYHGKDAPEISDADYDALKRRNDAIEAA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHH
FPALVRADSPSKKVGFTPLPTFAPIVHARPMLSLDNTFSEEDLRDFVSSVYRFLGHLPDD
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SIAFTAEPKIDGLSMSIRYENRKLKTAATRGDGTTGENVTANIRTIKEIPNELPADAPDV
CEEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCH
VEVRGEVYMAKSDFLALNAQMEADGKQTYVNPRNTASGSLRQLDANVTAKRKLRFFAYAL
HEECCCEEEEECCEEEEEEEECCCCCEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
GEVSNGGQPARIADTQYGIVEKFREWGFPVNPLMKRFTSAQQLLEHYNEIGVARPDLDYD
HHCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IDGVVYKVDRLDLQERLGFRSRSPRWATAHKFPAEQAFTTVENIEIQVGRTGALTPVARL
CCCEEEEEHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHH
TPITVGGVVVTNATLHNADYIEGIGNSGERIRPEDHDIRIGDTVIVQRAGDVIPQVLDVL
CCEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHH
LEKRAAGAAKYVFPEKCPVCGSHAVRERNEKTGKLDSVTRCTGGFVCRAQAVEHLKHFVS
HHHHHCCCEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
RNAFDIEGLGTKQIDFFFESDDPALSIKTAPDIFTLKARQEASHLTKLENIDGFGKVSVK
CCCEEECCCCCCEEEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCHHHH
KLFDAIDARRAIDLHRLIFALGIRHVGETTAKLLARSYGTYEHFEKAMKAAADPASDAWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
ELNSIDGIGEVVARAIIEFYKEPRNLDVIDRLIRELQPKEAEKPSTEGSPVAGKTVVFTG
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
SLEKFTRDEAKARAESLGAKVAGSVSKKTDILVAGPGAGSKLAKATELGVQTMDEDEWLA
CHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEE
LIGG
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194