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The map label for this gene is xthA [H]

Identifier: 15889021

GI number: 15889021

Start: 1701558

End: 1702355

Strand: Direct

Name: xthA [H]

Synonym: Atu1714

Alternate gene names: 15889021

Gene position: 1701558-1702355 (Clockwise)

Preceding gene: 15889020

Following gene: 159184886

Centisome position: 59.88

GC content: 59.15

Gene sequence:

>798_bases
ATGAAGATTGCCACCTGGAACATCAATGGCGTCAAGGCGCGTATCGAAAACCTCTGCCAGTGGCTGAAGGATTCCTCGCC
TGACATCGTCTGCCTTCAGGAAATCAAGTCGGTCGACGAGGGTTTCCCCCGCCTTGAGATCGAGGCGCTCGGTTATCACA
TCGAAACCCACGGCCAGAAGGGTTTTAACGGCGTCGCCCTGCTCTCGAAGGTGAAGCCGGACGAGGTCAATCGTGGCCTG
CCGGGCGATGATGCCGATGAACAGGCCCGTTTCATCGAAGGCGTGTTTTCCGTGGAAGGCGGCGCGATCCGCGTCTGCTC
GCTTTACCTGCCGAACGGCAATCCGCCTGACGATCCGGTCAAATATCCCTACAAGCTTGCCTGGATGGAGCGCCTGCGGC
GCTTTGCGGAAGATCGCCTTGCAATGGAAGAGCCGCTGATCCTCGCCGGCGATTACAACGTCATCCCCGAACCCTTCGAT
TGCCACGACCCACGCGTGTGGGAAGGCGACGCATTGTTCCTGCCGAAGACCCGGGCGGCCTTCCGCAAACTGGAAAATCT
GGGCTTCACTGACGCCGCGCGTGCGACGACCGACGCGGCAGGACTTTATTCCTTCTGGGATTATCAGGCCGGCGCCTGGC
CGAAAAACAACGGCATCCGCATCGATCATCTGATGCTGTCAGCGGAAGCAGCCGACAGGCTGCAATCGGTCGAGATCGAA
AAACATGTGCGGGCCTGGGAAAAACCGTCCGACCATGTGCCGGTCTGCGGTTATTTCGATTTCAGTCCGATCGGCTGA

Upstream 100 bases:

>100_bases
ATTCTCGCCTCTTTCCACCACATCCGCACCGGCACCTTCCAAAAACGGTGGCAATCGCTTTTGCATCGGTTAAAAGTACC
CCCACAAAAGGGAAACTCCG

Downstream 100 bases:

>100_bases
ACCGTTGCTGTCGGGCGGCTGAAACCGTCCCGACCGGCATTCTCAATCCGGATCGTTGAACTGCATGTTCTGCGCCATGG
CGATGGCGGCGCGGCGGTCG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKIATWNINGVKARIENLCQWLKDSSPDIVCLQEIKSVDEGFPRLEIEALGYHIETHGQKGFNGVALLSKVKPDEVNRGL
PGDDADEQARFIEGVFSVEGGAIRVCSLYLPNGNPPDDPVKYPYKLAWMERLRRFAEDRLAMEEPLILAGDYNVIPEPFD
CHDPRVWEGDALFLPKTRAAFRKLENLGFTDAARATTDAAGLYSFWDYQAGAWPKNNGIRIDHLMLSAEAADRLQSVEIE
KHVRAWEKPSDHVPVCGYFDFSPIG

Sequences:

>Translated_265_residues
MKIATWNINGVKARIENLCQWLKDSSPDIVCLQEIKSVDEGFPRLEIEALGYHIETHGQKGFNGVALLSKVKPDEVNRGL
PGDDADEQARFIEGVFSVEGGAIRVCSLYLPNGNPPDDPVKYPYKLAWMERLRRFAEDRLAMEEPLILAGDYNVIPEPFD
CHDPRVWEGDALFLPKTRAAFRKLENLGFTDAARATTDAAGLYSFWDYQAGAWPKNNGIRIDHLMLSAEAADRLQSVEIE
KHVRAWEKPSDHVPVCGYFDFSPIG
>Mature_265_residues
MKIATWNINGVKARIENLCQWLKDSSPDIVCLQEIKSVDEGFPRLEIEALGYHIETHGQKGFNGVALLSKVKPDEVNRGL
PGDDADEQARFIEGVFSVEGGAIRVCSLYLPNGNPPDDPVKYPYKLAWMERLRRFAEDRLAMEEPLILAGDYNVIPEPFD
CHDPRVWEGDALFLPKTRAAFRKLENLGFTDAARATTDAAGLYSFWDYQAGAWPKNNGIRIDHLMLSAEAADRLQSVEIE
KHVRAWEKPSDHVPVCGYFDFSPIG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=30.8270676691729, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=30.8270676691729, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=30.8270676691729, Blast_Score=117, Evalue=9e-27,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=36.4661654135338, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=29.6992481203008, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=29.6992481203008, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29819; Mature: 29819

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00063 ALDOKETO_REDUCTASE_3 ; PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINGVKARIENLCQWLKDSSPDIVCLQEIKSVDEGFPRLEIEALGYHIETHGQK
CEEEEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEEEEEEEECCCC
GFNGVALLSKVKPDEVNRGLPGDDADEQARFIEGVFSVEGGAIRVCSLYLPNGNPPDDPV
CCCHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
KYPYKLAWMERLRRFAEDRLAMEEPLILAGDYNVIPEPFDCHDPRVWEGDALFLPKTRAA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEECCCEEECCCHHHH
FRKLENLGFTDAARATTDAAGLYSFWDYQAGAWPKNNGIRIDHLMLSAEAADRLQSVEIE
HHHHHHCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEHEEEEHHHHHHHHHHHHH
KHVRAWEKPSDHVPVCGYFDFSPIG
HHHHHHCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKIATWNINGVKARIENLCQWLKDSSPDIVCLQEIKSVDEGFPRLEIEALGYHIETHGQK
CEEEEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEEEEEEEEECCCC
GFNGVALLSKVKPDEVNRGLPGDDADEQARFIEGVFSVEGGAIRVCSLYLPNGNPPDDPV
CCCHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
KYPYKLAWMERLRRFAEDRLAMEEPLILAGDYNVIPEPFDCHDPRVWEGDALFLPKTRAA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEECCCEEECCCHHHH
FRKLENLGFTDAARATTDAAGLYSFWDYQAGAWPKNNGIRIDHLMLSAEAADRLQSVEIE
HHHHHHCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEHEEEEHHHHHHHHHHHHH
KHVRAWEKPSDHVPVCGYFDFSPIG
HHHHHHCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]