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The map label for this gene is nadE [H]

Identifier: 15888965

GI number: 15888965

Start: 1641644

End: 1643323

Strand: Reverse

Name: nadE [H]

Synonym: Atu1657

Alternate gene names: 15888965

Gene position: 1643323-1641644 (Counterclockwise)

Preceding gene: 159184863

Following gene: 159184859

Centisome position: 57.83

GC content: 59.88

Gene sequence:

>1680_bases
ATGAGCGACAGACACGATATCCAGAACCATCTTCGAATTGCCGTCGGGCAGTTCAATCCGACTGTTGGCGACGTGGCGGG
CAATCTCGCCAGGGCGCGTGAGGCACGGGCCGATGCGGCGACGCAGGGTGCCGACCTTCTGCTCCTGACGGAGCTGTTCA
TTTCAGGCTATCCGCCGGAGGATCTGGTGCTTAAGCCGGCTTTTCTGAAGGCCTGCCTGAAGGCTGTGGAAGAACTGGCG
GCGGAGACTGCCGATGGCGGGCCGGGCGTCGTCATCGGTTTTCCGCGTCAGGGCGAGACAGGCCGGCACAATTCGGTGGC
GCTTCTGGATGGTGGCAAGATCATCGCGTTGCGTGACAAGATCGATCTGCCGAATTACGGCGAGTTCGACGAAAAACGTG
TTTTCGAAGAAGGGTCGATTTCCGGGCCCTATAATTTCCGTGGCGTGCGGATCGGCATTCCGATCTGCGAGGAAATCTGG
AACGACATGGGCGTGTGCGAGACGCTGGCCGAAAGCGGGGCGGAAATCCTGCTGGTGCCGAATGGGTCGCCCTATTATCG
CGGCAAGCTGGATGTGCGCCATCAGGTGGCGCTCAGGCAGGTGATCGAAAGCGGCCTGCCGCTGGTTTTCGCCAATCAGC
TGGGCGGACAGGATGAGCTGGTGTTTGACGGTGCGAGCTTCGGCTTTAATGCCGACAAGACGCTGGCCTTTCAGATGAGC
CAGTTCGAGGCCACGCTCGCTGTCACCGACTGGAAACGCACCGCAGACGGCTGGCATTGCGACAGCGGGCCTTTCTCGAA
AATCCCTGAGGGCGAGGAGGCGGATTACCGCGCCTGCATGCTGGGCTTTAGGGATTACGTCAACAAGAACGGCTTCAAGA
GCGTGGTTCTCGGCCTTTCCGGCGGCATCGATTCGGCGATCTGCGCAGCCCTTGCCGTCGATGCGCTGGGTGAGGAGCGG
GTACGCTGCATCATGCTGCCCTACCGCTACACCTCGGAAGAATCGCTGAAGGACGCCGCCGATTGTGCGAGAGCGCTCGG
CTGCCGTTACGATATCGTGCCGATCGTCGAGCCGGTGGAAGGCTTTCTTTCGGCGCTCTCCGAACTCTTCGAGGGCACGG
AAGAGGGCATTACCGAAGAAAATCTGCAGAGCCGCACGCGTGGCACCATTTTGATGGCTGTCTCCAACAAGTTCGGCTCG
ATGGTGGTAACGACCGGCAACAAGTCGGAAATGTCGGTGGGCTATGCGACGCTTTATGGCGATATGAATGGCGGTTTCAA
TCCCATCAAGGACCTCTACAAGATGCAGGTCTATGCCATTTCCAGCTGGCGCAACGCCCATGTGCCGCCGGGCGCGCTTG
GCCCTTCCGGCGAGGTTATCCCAGCCAATATCATCGCCAAGGCGCCATCGGCCGAACTGCGCCCCAACCAGACCGACCAG
GATTCGCTGCCGCCCTATCCGGTGCTGGATGATATTCTCGAGTGCCTTGTCGAGAAGGAAATGTCGGTCGAGGAAATTCT
GGCGCGCGGCCATGATGTGGCCACCGTGCACCGGGTCGAGCATCTGCTTTATCTGGCCGAATACAAGCGCCGGCAATCGG
CTCCGGGTGTGAAGATCACCAAGAAGAATTTTGGGCGGGACCGGCGTTATCCGATTACCAACCGGTTTCGGGATCGGTAA

Upstream 100 bases:

>100_bases
GGTGATAACCTTAGCGTGAACGCAGCCGTGGCGTCTTGCCCAGGCGACGGCATGCAATTGCCGCGTTGCGGAGCGGCCAC
GGGCGCGGTATTGAAATGGC

Downstream 100 bases:

>100_bases
GGTGCGCAAACGGAATGTATGCGCCAGTCGACGCTCCACACCAGACTATCGACAGGCCCGGGAGGCAGCATGCGCAGTTC
CATCGAAATCTTCAACATCC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSDRHDIQNHLRIAVGQFNPTVGDVAGNLARAREARADAATQGADLLLLTELFISGYPPEDLVLKPAFLKACLKAVEELA
AETADGGPGVVIGFPRQGETGRHNSVALLDGGKIIALRDKIDLPNYGEFDEKRVFEEGSISGPYNFRGVRIGIPICEEIW
NDMGVCETLAESGAEILLVPNGSPYYRGKLDVRHQVALRQVIESGLPLVFANQLGGQDELVFDGASFGFNADKTLAFQMS
QFEATLAVTDWKRTADGWHCDSGPFSKIPEGEEADYRACMLGFRDYVNKNGFKSVVLGLSGGIDSAICAALAVDALGEER
VRCIMLPYRYTSEESLKDAADCARALGCRYDIVPIVEPVEGFLSALSELFEGTEEGITEENLQSRTRGTILMAVSNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYAISSWRNAHVPPGALGPSGEVIPANIIAKAPSAELRPNQTDQ
DSLPPYPVLDDILECLVEKEMSVEEILARGHDVATVHRVEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR

Sequences:

>Translated_559_residues
MSDRHDIQNHLRIAVGQFNPTVGDVAGNLARAREARADAATQGADLLLLTELFISGYPPEDLVLKPAFLKACLKAVEELA
AETADGGPGVVIGFPRQGETGRHNSVALLDGGKIIALRDKIDLPNYGEFDEKRVFEEGSISGPYNFRGVRIGIPICEEIW
NDMGVCETLAESGAEILLVPNGSPYYRGKLDVRHQVALRQVIESGLPLVFANQLGGQDELVFDGASFGFNADKTLAFQMS
QFEATLAVTDWKRTADGWHCDSGPFSKIPEGEEADYRACMLGFRDYVNKNGFKSVVLGLSGGIDSAICAALAVDALGEER
VRCIMLPYRYTSEESLKDAADCARALGCRYDIVPIVEPVEGFLSALSELFEGTEEGITEENLQSRTRGTILMAVSNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYAISSWRNAHVPPGALGPSGEVIPANIIAKAPSAELRPNQTDQ
DSLPPYPVLDDILECLVEKEMSVEEILARGHDVATVHRVEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR
>Mature_558_residues
SDRHDIQNHLRIAVGQFNPTVGDVAGNLARAREARADAATQGADLLLLTELFISGYPPEDLVLKPAFLKACLKAVEELAA
ETADGGPGVVIGFPRQGETGRHNSVALLDGGKIIALRDKIDLPNYGEFDEKRVFEEGSISGPYNFRGVRIGIPICEEIWN
DMGVCETLAESGAEILLVPNGSPYYRGKLDVRHQVALRQVIESGLPLVFANQLGGQDELVFDGASFGFNADKTLAFQMSQ
FEATLAVTDWKRTADGWHCDSGPFSKIPEGEEADYRACMLGFRDYVNKNGFKSVVLGLSGGIDSAICAALAVDALGEERV
RCIMLPYRYTSEESLKDAADCARALGCRYDIVPIVEPVEGFLSALSELFEGTEEGITEENLQSRTRGTILMAVSNKFGSM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYAISSWRNAHVPPGALGPSGEVIPANIIAKAPSAELRPNQTDQD
SLPPYPVLDDILECLVEKEMSVEEILARGHDVATVHRVEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=261, Percent_Identity=28.3524904214559, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61177; Mature: 61046

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRHDIQNHLRIAVGQFNPTVGDVAGNLARAREARADAATQGADLLLLTELFISGYPPE
CCCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
DLVLKPAFLKACLKAVEELAAETADGGPGVVIGFPRQGETGRHNSVALLDGGKIIALRDK
HCEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEECC
IDLPNYGEFDEKRVFEEGSISGPYNFRGVRIGIPICEEIWNDMGVCETLAESGAEILLVP
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEE
NGSPYYRGKLDVRHQVALRQVIESGLPLVFANQLGGQDELVFDGASFGFNADKTLAFQMS
CCCCCEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEHH
QFEATLAVTDWKRTADGWHCDSGPFSKIPEGEEADYRACMLGFRDYVNKNGFKSVVLGLS
HHEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEECC
GGIDSAICAALAVDALGEERVRCIMLPYRYTSEESLKDAADCARALGCRYDIVPIVEPVE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GFLSALSELFEGTEEGITEENLQSRTRGTILMAVSNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCHHCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYAISSWRNAHVPPGALGPSGEVIPANIIAKAPSAELRPNQTDQ
CCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEHHHEECCCCCCCCCCCCCC
DSLPPYPVLDDILECLVEKEMSVEEILARGHDVATVHRVEHLLYLAEYKRRQSAPGVKIT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KKNFGRDRRYPITNRFRDR
HHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SDRHDIQNHLRIAVGQFNPTVGDVAGNLARAREARADAATQGADLLLLTELFISGYPPE
CCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
DLVLKPAFLKACLKAVEELAAETADGGPGVVIGFPRQGETGRHNSVALLDGGKIIALRDK
HCEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEECC
IDLPNYGEFDEKRVFEEGSISGPYNFRGVRIGIPICEEIWNDMGVCETLAESGAEILLVP
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEE
NGSPYYRGKLDVRHQVALRQVIESGLPLVFANQLGGQDELVFDGASFGFNADKTLAFQMS
CCCCCEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEEEHH
QFEATLAVTDWKRTADGWHCDSGPFSKIPEGEEADYRACMLGFRDYVNKNGFKSVVLGLS
HHEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEECC
GGIDSAICAALAVDALGEERVRCIMLPYRYTSEESLKDAADCARALGCRYDIVPIVEPVE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GFLSALSELFEGTEEGITEENLQSRTRGTILMAVSNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCHHCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYAISSWRNAHVPPGALGPSGEVIPANIIAKAPSAELRPNQTDQ
CCCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEHHHEECCCCCCCCCCCCCC
DSLPPYPVLDDILECLVEKEMSVEEILARGHDVATVHRVEHLLYLAEYKRRQSAPGVKIT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KKNFGRDRRYPITNRFRDR
HHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]