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The map label for this gene is tpiA

Identifier: 15888933

GI number: 15888933

Start: 1604904

End: 1605674

Strand: Reverse

Name: tpiA

Synonym: Atu1620

Alternate gene names: 15888933

Gene position: 1605674-1604904 (Counterclockwise)

Preceding gene: 15888934

Following gene: 159184841

Centisome position: 56.51

GC content: 61.48

Gene sequence:

>771_bases
ATGACGCCGAATGTTCGACCGCTTGTTGCCGGAAACTGGAAGATGAACGGCACCCGTGCCTCGCTCGATCAGATCAAGGC
CATGGCAGAGGGCGTTAAGGGCGCGCTGTCCGAGAAGGTGGATGCGCTGATCTGCCCGCCCTCGACATTGCTCTACGTAG
CAACGGCGCTCTGCGATGACAGCCCGCTGCTGATCGGCGCGCAGGATTGCCACCAGAATGATACCGGCGCCCATACCGGC
GATATTTCCGCCGAGATGATTGCCGATTGCTTCGGCACCCATGTTATCGTTGGCCATTCCGAGCGCCGCACCGACCACGC
CGAGACCGATCACCTGGTTCGCGCCAAGGCATCCGCTGCCCATCAGGTCGACCTTATCGCCATCGTCTGCATCGGCGAGA
CTGCGGATGAGCGCAAGGCGGGACAGACGCTGGATATTCTCAAGCGCCAGTTGGCCGGCTCCCTGCCGGATGAAGCGACT
GCTGAAAACACCGTCATCGCCTATGAGCCTGTGTGGGCGATTGGCACCGGTCTTACCCCGACCACGCAGGACGTGCGCGA
AGCCCATGCCTTCATGCGCGACGAGCTGGTCAAGCGCTTCGGTGACGCCGGCAAGACCATGCGCATTCTCTATGGTGGTT
CGGTGAAGCCCGCCAATGCGCTGGAACTGATGGGCGTCGAGAATGTCGATGGCGCGCTGATCGGCGGCGCCAGCTTGAAA
GCCGCAGACTTCCTTTCCATCTATGCGGCATATGAGCAACTGACGGCCTGA

Upstream 100 bases:

>100_bases
GGATTTCCGGCAGGGTTTCTGTCGTGATTGAGGGCCGCGTGCGGCCGAGGGATGGGGGTGCCGCCAAAAGCGGGCCAGAG
ACAAAGGGAATGGGATACCT

Downstream 100 bases:

>100_bases
GACCAGCTTTCCTGTCACAGCGGCTGGAAAGGGGCTTGGAATAAACCTTCGCCTCATGTAAAGAGCCGCGAAACCTTCTT
TGTAGTGCTCCGCGCGGGGC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTPNVRPLVAGNWKMNGTRASLDQIKAMAEGVKGALSEKVDALICPPSTLLYVATALCDDSPLLIGAQDCHQNDTGAHTG
DISAEMIADCFGTHVIVGHSERRTDHAETDHLVRAKASAAHQVDLIAIVCIGETADERKAGQTLDILKRQLAGSLPDEAT
AENTVIAYEPVWAIGTGLTPTTQDVREAHAFMRDELVKRFGDAGKTMRILYGGSVKPANALELMGVENVDGALIGGASLK
AADFLSIYAAYEQLTA

Sequences:

>Translated_256_residues
MTPNVRPLVAGNWKMNGTRASLDQIKAMAEGVKGALSEKVDALICPPSTLLYVATALCDDSPLLIGAQDCHQNDTGAHTG
DISAEMIADCFGTHVIVGHSERRTDHAETDHLVRAKASAAHQVDLIAIVCIGETADERKAGQTLDILKRQLAGSLPDEAT
AENTVIAYEPVWAIGTGLTPTTQDVREAHAFMRDELVKRFGDAGKTMRILYGGSVKPANALELMGVENVDGALIGGASLK
AADFLSIYAAYEQLTA
>Mature_255_residues
TPNVRPLVAGNWKMNGTRASLDQIKAMAEGVKGALSEKVDALICPPSTLLYVATALCDDSPLLIGAQDCHQNDTGAHTGD
ISAEMIADCFGTHVIVGHSERRTDHAETDHLVRAKASAAHQVDLIAIVCIGETADERKAGQTLDILKRQLAGSLPDEATA
ENTVIAYEPVWAIGTGLTPTTQDVREAHAFMRDELVKRFGDAGKTMRILYGGSVKPANALELMGVENVDGALIGGASLKA
ADFLSIYAAYEQLTA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=39.5256916996047, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=39.5256916996047, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=41.1067193675889, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17536593, Length=245, Percent_Identity=42.4489795918367, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6320255, Length=242, Percent_Identity=42.5619834710744, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=41.5322580645161, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=41.5322580645161, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=41.2955465587045, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_AGRT5 (Q8UEY3)

Other databases:

- EMBL:   AE007869
- PIR:   AH2775
- PIR:   F97555
- RefSeq:   NP_354614.1
- ProteinModelPortal:   Q8UEY3
- SMR:   Q8UEY3
- STRING:   Q8UEY3
- GeneID:   1133658
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1620
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q8UEY3
- ProtClustDB:   PRK00042
- BioCyc:   ATUM176299-1:ATU1620-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27138; Mature: 27007

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 169-169 BINDING 12-12 BINDING 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNVRPLVAGNWKMNGTRASLDQIKAMAEGVKGALSEKVDALICPPSTLLYVATALCDD
CCCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCC
SPLLIGAQDCHQNDTGAHTGDISAEMIADCFGTHVIVGHSERRTDHAETDHLVRAKASAA
CCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCC
HQVDLIAIVCIGETADERKAGQTLDILKRQLAGSLPDEATAENTVIAYEPVWAIGTGLTP
CCEEEEEEEEECCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEECCCCCC
TTQDVREAHAFMRDELVKRFGDAGKTMRILYGGSVKPANALELMGVENVDGALIGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCH
AADFLSIYAAYEQLTA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPNVRPLVAGNWKMNGTRASLDQIKAMAEGVKGALSEKVDALICPPSTLLYVATALCDD
CCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCC
SPLLIGAQDCHQNDTGAHTGDISAEMIADCFGTHVIVGHSERRTDHAETDHLVRAKASAA
CCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCC
HQVDLIAIVCIGETADERKAGQTLDILKRQLAGSLPDEATAENTVIAYEPVWAIGTGLTP
CCEEEEEEEEECCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEECCCCCC
TTQDVREAHAFMRDELVKRFGDAGKTMRILYGGSVKPANALELMGVENVDGALIGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCH
AADFLSIYAAYEQLTA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194