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The map label for this gene is fixI [H]

Identifier: 15888852

GI number: 15888852

Start: 1519351

End: 1521642

Strand: Reverse

Name: fixI [H]

Synonym: Atu1528

Alternate gene names: 15888852

Gene position: 1521642-1519351 (Counterclockwise)

Preceding gene: 15888853

Following gene: 159184801

Centisome position: 53.55

GC content: 63.79

Gene sequence:

>2292_bases
ATGAGCTGCTGCGCACCGGGAACGGAAGGTTCGCTGGAGCTCGGCGAACCGCTTAATCCGCCATCGTCGGAGGAGTTGCT
GCTGGCGAGCCGCGATCTGGGGCAGGGGTTGCGCCAGACCGATCTTAGCGTGCCCGGTGTTTATTGCGGCGCCTGCATCA
CCACCATCGAGACCGCGCTCGGCCGTCTCGGCCAAGTGGAGCGGGCGCGGGTGAACCTGTCTTCGAAACGTGTGGCGGTT
GTCTGGAAAGAAGAGGTCAATGGCGTCAGGACCGATCCCGCCGACATTGCCCGCGCCATCCTTGCGACGGGATATCGCAT
ACATCTTTTCGCCAGCGGGCAGGATGCTTCATACGCCTTGCGCTCGCAGCTGATCAGGGCGGTGGCGCTGTGCGGCTTTG
CTTCCGCCAATATCATGCTTCTGTCGGTCTCCGTCTGGTCGGGGGCGGATGCCGCGACGCGTGACATGTTCCACTGGATA
TCGGCGATGATCGCCGCGCCGGCGCTGATCTATGGCGGGCGTTTCTTTTACCAGTCGGCCTGGAACGCGCTGAAACACGG
CCGCACCAACATGGATGTGCCGATTGCGCTCGCCATCACGCTCTCCTATGCCGTTTCGCTGTGGGAGACCATTCATCACG
GCGAACATGCGTGGTTCGATGCGACCGTGTCGCTGCTGTTCTTCCTGCTGATCGGCCGCACGCTGGACCATATCATGCGC
GACAAGGCGCGCTCCGCCATTGCCGGATTGGCCCGGCTTTCGCCGCGAGGGGCAACGGTGATCGATGCAGACGGGGTCCG
GGACTACCGGCCGCTTGCGGATATCGAACCTGGAATGTCGATTGCGATTGCGGCGGGTGACCGGGTGGCGGTGGATGCCG
TGGTGGTCTGCGGCAGCAGCGATCTCGACATGTCCATCGTCAATGGTGAAAGCGCGCCGCGCCGCGTCGTGGCCGGCGAC
AGCCTGCAGGCCGGCACGCTCAATCTCACCGGGTCGCTTGTCGCCCGGGTAACCGCGTCCGCAAAGGATTCTTTCCTGTC
GGAAGTGATCGGGTTGATGGAGGCGGCCGAAGGCGGGCGCGCGCGTTACCGGCGTATCGCCGACCGTGCCGCGAGCTATT
ATTCGCCCGTGGTGCATCTTCTGGCGCTGGTGACGTTTCTCGGCTGGGGCATTTTCGGCGGTGACTGGAAACAGGCGATG
CTGATCGCCATTGCTGTTCTCATCATAACCTGCCCCTGCGCGCTCGGCCTTGCTGTTCCAGTCGTGCAGGTGGTGGCCGC
AGGAAGGCTTTTTCGGCGTGGCATCATGGTCAAGGAAGGCTCGGCCATGGAGCGTCTTTCGGAGATCGATACCGTGCTGT
TCGACAAGACCGGGACCTTGACCGTCGGCAAGCCTAGGCTGGTCGAGACCGCAGATGTGAAGCCGGCCATCATGGCGATT
GCGGCGGGGCTCGCTGCCCATTCGCGCCATCCTCTCTCGAAAGCCCTGCATGCCGCCTATAATGGTGCCTTGCCAGCCTA
TGAGGCGGTGCGGGAAATTCCGGGGTCGGGTGTCGAGGCCGAGACGGAAGCCGGCACTTACCGGCTCGGCAATCGCCGTT
TCGCCTGCCCGGATGAGACACGGATCAACAATGGCGATGCGCGGTCGGAAGTGGTGCTGTCTCTCGACGGCCGGCATCTG
GCGAGCTTCGGCTTCGAGGACAATCCGCGCGCGGGTGCTATTGCGGCATTGCGCAGCCTGTCTGTGCGGGGCCTTGCGCA
GGAAATCGTCTCGGGTGACCGGGCCGCCGCCGTCAGTGCGATGGCGCAGCGGCTGGGGATCGGCAACTGGCGCGCGGACC
TGTCGCCGAAGGACAAGGCGGCGCGCTGTGCCGCGCTTGCCGGCGAGGGGCACAGGGTGCTGATGGTCGGGGATGGTATC
AACGACGCGCCAGCGCTTGCCGCCGCCCACGTATCTATGGCGCCCGCCACCGCCGCCGATATTGGCCGGCAGGCCGCCGA
TTTCGTGTTTATGCAGGAAGATCTCGATGCCGTACCTTTCGCGATCGAGACGTCGCAGCGAGCCGGCAACCTCATCCGGC
AGAACTTTGCGCTTGCCATCGGCTACAATATCATCGCCGTACCGATTGCGATTGCCGGTTATGCGACGCCGATGATTGCG
GCCATTGCCATGTCGACATCTTCGCTGATCGTCGTTGCCAATGCGCTGCGCCTTGCCGGTGCTGCTGACACTATTCTCCC
TCAGAAGGAAGCGAAGTCGGGATTTACGGGCGAGGGGGCGCAACTGGCATGA

Upstream 100 bases:

>100_bases
ACATGTTGCCCGGTCAGTGGATCGTCGAGGTCACCGCCGTCAAGGAAGGCAGGATCATCGTGCATGAGGGCACGCGGATC
GCCGTCATCAGGGGACGCAA

Downstream 100 bases:

>100_bases
ACATGCTGATCTATCTCATTCCCGTCGCGCTGTTCCTGGGCGCGCTCGGGCTTTTCGCCTTCCTCTGGTCGGTGCGCTCA
GGTCAATATGAGGATATGGA

Product: nitrogen fixation protein FixI

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MSCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETALGRLGQVERARVNLSSKRVAV
VWKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYALRSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWI
SAMIAAPALIYGGRFFYQSAWNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMR
DKARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSSDLDMSIVNGESAPRRVVAGD
SLQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGRARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAM
LIAIAVLIITCPCALGLAVPVVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAI
AAGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDETRINNGDARSEVVLSLDGRHL
ASFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSAMAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGI
NDAPALAAAHVSMAPATAADIGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIA
AIAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA

Sequences:

>Translated_763_residues
MSCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETALGRLGQVERARVNLSSKRVAV
VWKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYALRSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWI
SAMIAAPALIYGGRFFYQSAWNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMR
DKARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSSDLDMSIVNGESAPRRVVAGD
SLQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGRARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAM
LIAIAVLIITCPCALGLAVPVVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAI
AAGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDETRINNGDARSEVVLSLDGRHL
ASFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSAMAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGI
NDAPALAAAHVSMAPATAADIGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIA
AIAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA
>Mature_762_residues
SCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETALGRLGQVERARVNLSSKRVAVV
WKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYALRSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWIS
AMIAAPALIYGGRFFYQSAWNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMRD
KARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSSDLDMSIVNGESAPRRVVAGDS
LQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGRARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAML
IAIAVLIITCPCALGLAVPVVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAIA
AGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDETRINNGDARSEVVLSLDGRHLA
SFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSAMAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGIN
DAPALAAAHVSMAPATAADIGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIAA
IAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI115529486, Length=833, Percent_Identity=26.7707082833133, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI55743071, Length=788, Percent_Identity=27.0304568527919, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI55743073, Length=551, Percent_Identity=27.4047186932849, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI48762691, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI48762687, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI48762689, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI48762685, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI1786691, Length=738, Percent_Identity=32.520325203252, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1789879, Length=714, Percent_Identity=27.3109243697479, Blast_Score=207, Evalue=3e-54,
Organism=Escherichia coli, GI1786914, Length=458, Percent_Identity=25.9825327510917, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556548, Length=659, Percent_Identity=30.6525037936267, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6320475, Length=625, Percent_Identity=27.84, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319772, Length=580, Percent_Identity=27.9310344827586, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6325221, Length=562, Percent_Identity=23.3096085409253, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321430, Length=554, Percent_Identity=22.2021660649819, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI221329854, Length=553, Percent_Identity=28.9330922242315, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 80255; Mature: 80124

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETAL
CCCCCCCCCCCCCCCCCCCCCCCCHHEEECHHHHCCHHHCCCCCCCHHHHHHHHHHHHHH
GRLGQVERARVNLSSKRVAVVWKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYAL
HHHCCHHHHHCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHH
RSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWISAMIAAPALIYGGRFFYQSA
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
WNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSS
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEECCC
DLDMSIVNGESAPRRVVAGDSLQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGR
CCCEEEECCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAMLIAIAVLIITCPCALGLAVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAI
HHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCEECCCCCEEECCCCCHHHHHH
AAGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDET
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCEECCCCCC
RINNGDARSEVVLSLDGRHLASFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSA
CCCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGINDAPALAAAHVSMAPATAAD
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHH
IGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIA
HHHHHHHHEEECCCCCCCCEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
AIAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA
HHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETAL
CCCCCCCCCCCCCCCCCCCCCCCHHEEECHHHHCCHHHCCCCCCCHHHHHHHHHHHHHH
GRLGQVERARVNLSSKRVAVVWKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYAL
HHHCCHHHHHCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHH
RSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWISAMIAAPALIYGGRFFYQSA
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
WNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DKARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSS
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEECCC
DLDMSIVNGESAPRRVVAGDSLQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGR
CCCEEEECCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAMLIAIAVLIITCPCALGLAVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAI
HHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCEECCCCCEEECCCCCHHHHHH
AAGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDET
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCEECCCCCC
RINNGDARSEVVLSLDGRHLASFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSA
CCCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGINDAPALAAAHVSMAPATAAD
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHH
IGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIA
HHHHHHHHEEECCCCCCCCEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
AIAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA
HHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]