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The map label for this gene is dusA [H]

Identifier: 15888840

GI number: 15888840

Start: 1506173

End: 1507183

Strand: Reverse

Name: dusA [H]

Synonym: Atu1515

Alternate gene names: 15888840

Gene position: 1507183-1506173 (Counterclockwise)

Preceding gene: 15888843

Following gene: 159184791

Centisome position: 53.04

GC content: 59.94

Gene sequence:

>1011_bases
ATGTACAGACAAGCGCTTGCCAGCGGCGAAAAAATCTTCGCCGTGGCCCCGATGATCGATTGGACCGATACGAGGTGCAG
ATTTCTGCATCGGCAACTGTCGAAGCGCGCTTTGCTGTTCACCGAGATGATCGTTGCCGACGCTATCATCCATGGCCAGC
GCGAGAAGTTGCTCGGTTACCACCCGCAGGAACATCCCGTTGCGCTGCAGCTCGGCGGATCGAATCCCGCCAAGCTTGCC
GAGGCGGTGCGGATTGCCGGTGACTACGGTTATGACGAGATCAATCTCAATGTCGGCTGTCCTTCGGACCGGGTGCAATC
CGGCACCTTCGGCGCCTGCCTGATGCGCGAGCCTGAAGTGGTGGCGCAATGTATTTCGGCTATGAAAGCGGTTGCGGCTG
TTCCGGTTACGGTCAAATGCCGCATCGGCGTTGATGACCAGGAGCCCGAAACGGTGCTGCCGGATTTTCTGGCGCGGGTC
GTGGCGGCCGGTGCGGATGCGGTCTGGGTCCATGCGCGAAAAGCCTGGCTGCAGGGGCTTTCGCCGAAGGAAAACCGCGA
GGTGCCGCCGCTTGATTACGATCTCGTCTACCGCATGAAGCGGGACAATCCTGACGTCTTCATCGGCATCAATGGTGGCA
TCGCCGATCTCGATCAGGCGGGTGAGCACCTGAAATACGTGGATGGCGTGATGCTCGGCAGGGCCGCCTATCACAACACC
TCGATCCTTGCCGATGTCGATCATCGTATCCATGGCGAGGAAGCCCTCCAATATGACTGGATGGCTTTGCGGGACACGAT
GATGGCTTATGCTGCCGATTATATTGCCACAGGTGGACGTCTCAATCACGTTACCCGCCACATGGTCGGCCTGTTTCAGG
GCATGCCGGGCGCGCGCCGTTTCCGGCAAATCCTCTCCAGCGATGCCACCAGGCCGGGAGCGGGGCCGGAGGTGATCGAG
GCAGCTTTCGCCGCCATCGATTTCAACCCGATGAAAGAACTGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCCGCGATTCCGGGGCGATTTCCAGTTCAAAATGACCTTGCCTGCAAAACTGGCTTTTTACGGTCCTTTGTTGTAGAAGC
CGGCGCAAAGAGGTTTATCC

Downstream 100 bases:

>100_bases
GACGCCTCAGACCGCTTTTTGCAGTTCCTGCGCACCCACGGGCGCATTGATCTTGCCGCCGGTGATGAAGAAGGCAAAGA
CGTCGCGCTGGGTCTTTTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MYRQALASGEKIFAVAPMIDWTDTRCRFLHRQLSKRALLFTEMIVADAIIHGQREKLLGYHPQEHPVALQLGGSNPAKLA
EAVRIAGDYGYDEINLNVGCPSDRVQSGTFGACLMREPEVVAQCISAMKAVAAVPVTVKCRIGVDDQEPETVLPDFLARV
VAAGADAVWVHARKAWLQGLSPKENREVPPLDYDLVYRMKRDNPDVFIGINGGIADLDQAGEHLKYVDGVMLGRAAYHNT
SILADVDHRIHGEEALQYDWMALRDTMMAYAADYIATGGRLNHVTRHMVGLFQGMPGARRFRQILSSDATRPGAGPEVIE
AAFAAIDFNPMKELAG

Sequences:

>Translated_336_residues
MYRQALASGEKIFAVAPMIDWTDTRCRFLHRQLSKRALLFTEMIVADAIIHGQREKLLGYHPQEHPVALQLGGSNPAKLA
EAVRIAGDYGYDEINLNVGCPSDRVQSGTFGACLMREPEVVAQCISAMKAVAAVPVTVKCRIGVDDQEPETVLPDFLARV
VAAGADAVWVHARKAWLQGLSPKENREVPPLDYDLVYRMKRDNPDVFIGINGGIADLDQAGEHLKYVDGVMLGRAAYHNT
SILADVDHRIHGEEALQYDWMALRDTMMAYAADYIATGGRLNHVTRHMVGLFQGMPGARRFRQILSSDATRPGAGPEVIE
AAFAAIDFNPMKELAG
>Mature_336_residues
MYRQALASGEKIFAVAPMIDWTDTRCRFLHRQLSKRALLFTEMIVADAIIHGQREKLLGYHPQEHPVALQLGGSNPAKLA
EAVRIAGDYGYDEINLNVGCPSDRVQSGTFGACLMREPEVVAQCISAMKAVAAVPVTVKCRIGVDDQEPETVLPDFLARV
VAAGADAVWVHARKAWLQGLSPKENREVPPLDYDLVYRMKRDNPDVFIGINGGIADLDQAGEHLKYVDGVMLGRAAYHNT
SILADVDHRIHGEEALQYDWMALRDTMMAYAADYIATGGRLNHVTRHMVGLFQGMPGARRFRQILSSDATRPGAGPEVIE
AAFAAIDFNPMKELAG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=242, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=53.6507936507937, Blast_Score=344, Evalue=4e-96,
Organism=Escherichia coli, GI1789660, Length=247, Percent_Identity=28.7449392712551, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=181, Percent_Identity=26.5193370165746, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=29.2237442922374, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=239, Percent_Identity=25.5230125523013, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24580595, Length=254, Percent_Identity=28.3464566929134, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19920448, Length=254, Percent_Identity=28.3464566929134, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36938; Mature: 36938

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRQALASGEKIFAVAPMIDWTDTRCRFLHRQLSKRALLFTEMIVADAIIHGQREKLLGY
CCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
HPQEHPVALQLGGSNPAKLAEAVRIAGDYGYDEINLNVGCPSDRVQSGTFGACLMREPEV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCHHCCCCHHH
VAQCISAMKAVAAVPVTVKCRIGVDDQEPETVLPDFLARVVAAGADAVWVHARKAWLQGL
HHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC
SPKENREVPPLDYDLVYRMKRDNPDVFIGINGGIADLDQAGEHLKYVDGVMLGRAAYHNT
CCCCCCCCCCCCHHHHEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SILADVDHRIHGEEALQYDWMALRDTMMAYAADYIATGGRLNHVTRHMVGLFQGMPGARR
CEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHH
FRQILSSDATRPGAGPEVIEAAFAAIDFNPMKELAG
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MYRQALASGEKIFAVAPMIDWTDTRCRFLHRQLSKRALLFTEMIVADAIIHGQREKLLGY
CCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
HPQEHPVALQLGGSNPAKLAEAVRIAGDYGYDEINLNVGCPSDRVQSGTFGACLMREPEV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCHHCCCCHHH
VAQCISAMKAVAAVPVTVKCRIGVDDQEPETVLPDFLARVVAAGADAVWVHARKAWLQGL
HHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC
SPKENREVPPLDYDLVYRMKRDNPDVFIGINGGIADLDQAGEHLKYVDGVMLGRAAYHNT
CCCCCCCCCCCCHHHHEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SILADVDHRIHGEEALQYDWMALRDTMMAYAADYIATGGRLNHVTRHMVGLFQGMPGARR
CEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHH
FRQILSSDATRPGAGPEVIEAAFAAIDFNPMKELAG
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]