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The map label for this gene is fad [C]

Identifier: 15888744

GI number: 15888744

Start: 1413523

End: 1414320

Strand: Direct

Name: fad [C]

Synonym: Atu1417

Alternate gene names: 15888744

Gene position: 1413523-1414320 (Clockwise)

Preceding gene: 15888735

Following gene: 15888746

Centisome position: 49.74

GC content: 62.28

Gene sequence:

>798_bases
ATGAGCGAAAAAATGACAGAAGAATTTGTCTCTTACGAATTGCGGGGTGACATCGCCCTTGTTGGCCTCAACAGGCCACA
AAAGCGCAACGCCATCAGTGACCGTTTCGTTGAGGCTATTGCCGCCGCCGCCGCGCGGGCGGAAAACGAAGCCCGTGCCG
CCGTCATTTTCGGTCATGGCGATCATTTCTGCGCCGGTCTCGATCTTGGCGAACATATCCACAAGACGCCGATCGAAGGC
GTGCGCGGCTCCCGCCGCTGGCACGCCGTGTTCTCGCAGATCGAGCATGGCACCATACCGTGGGTTTCCGCCCTGCATGG
CGCGGTCGTCGGCGGTGGGTTGGAGCTGGCCGCCTCGACCCATCTGCGGGTTGCCGACGAAAGCGCCTTCTTTGCGTTGC
CCGAGGGCCAACGCGGCATTTTCGTTGGGGGAGGCGGATCCGTCCGCATCGCGCGGCTGCTGGGGGTCGCCCGCATGAGC
GACATGATGCTGACTGGACGGGTCGCTTCGTCCGCCGAAGCCGAACGCTGGAACCTTGTGCAATATGCTGTGCCGCGCGG
CGAAGCGCTTGCGAAGGCGCAGGAGCTCGCCACCATTGCAGCGTCCAATGCACCTTTGTCCAACTATGCCGTTATCAACG
CGCTTCCCCGCATTCAGGATATGGCCAAGGAGGACGGGCTGTTCGTCGAGTCCTTCATTTCCTCCTTCACGGCGGCCAGC
CCCGAAGCCGAAGAACGCCTGCGCGCCTTTCTTGAAAAACGCGCTACGCGGCTGAAAGCACCGGACACGACGGCCTGA

Upstream 100 bases:

>100_bases
TAATCTTTTGTCAAAAAAATGATTACAAGAAAATAATATCCTTGTAAACAAAATGCGGAAGATAGATTGTATATGAGACG
AAATTATCGGAGACCTCGCC

Downstream 100 bases:

>100_bases
CGGCACTGTCCTCAAGCCATCTCGCAATATCGCCTGAGAATAAGCTTTCTCAGGCGACATTGGCCAGCGTCCCGGTGGAG
GCATGTTTCTTCATCCACTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSEKMTEEFVSYELRGDIALVGLNRPQKRNAISDRFVEAIAAAAARAENEARAAVIFGHGDHFCAGLDLGEHIHKTPIEG
VRGSRRWHAVFSQIEHGTIPWVSALHGAVVGGGLELAASTHLRVADESAFFALPEGQRGIFVGGGGSVRIARLLGVARMS
DMMLTGRVASSAEAERWNLVQYAVPRGEALAKAQELATIAASNAPLSNYAVINALPRIQDMAKEDGLFVESFISSFTAAS
PEAEERLRAFLEKRATRLKAPDTTA

Sequences:

>Translated_265_residues
MSEKMTEEFVSYELRGDIALVGLNRPQKRNAISDRFVEAIAAAAARAENEARAAVIFGHGDHFCAGLDLGEHIHKTPIEG
VRGSRRWHAVFSQIEHGTIPWVSALHGAVVGGGLELAASTHLRVADESAFFALPEGQRGIFVGGGGSVRIARLLGVARMS
DMMLTGRVASSAEAERWNLVQYAVPRGEALAKAQELATIAASNAPLSNYAVINALPRIQDMAKEDGLFVESFISSFTAAS
PEAEERLRAFLEKRATRLKAPDTTA
>Mature_264_residues
SEKMTEEFVSYELRGDIALVGLNRPQKRNAISDRFVEAIAAAAARAENEARAAVIFGHGDHFCAGLDLGEHIHKTPIEGV
RGSRRWHAVFSQIEHGTIPWVSALHGAVVGGGLELAASTHLRVADESAFFALPEGQRGIFVGGGGSVRIARLLGVARMSD
MMLTGRVASSAEAERWNLVQYAVPRGEALAKAQELATIAASNAPLSNYAVINALPRIQDMAKEDGLFVESFISSFTAASP
EAEERLRAFLEKRATRLKAPDTTA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=28.8461538461538, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI20127408, Length=199, Percent_Identity=31.6582914572864, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI68989263, Length=229, Percent_Identity=30.1310043668122, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502327, Length=270, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=28.2608695652174, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=32.4324324324324, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=31.6, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790281, Length=125, Percent_Identity=30.4, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=28.7179487179487, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=28.8065843621399, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=195, Percent_Identity=30.2564102564103, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=265, Percent_Identity=25.2830188679245, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=195, Percent_Identity=30.2564102564103, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=195, Percent_Identity=30.2564102564103, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=27.2251308900524, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357171, Length=202, Percent_Identity=35.1485148514851, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653139, Length=247, Percent_Identity=30.3643724696356, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=26.5384615384615, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=26.5384615384615, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650670, Length=187, Percent_Identity=35.8288770053476, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=28.6995515695067, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571730, Length=138, Percent_Identity=31.8840579710145, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571729, Length=138, Percent_Identity=31.8840579710145, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921000, Length=147, Percent_Identity=36.0544217687075, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24583077, Length=147, Percent_Identity=36.0544217687075, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24583079, Length=147, Percent_Identity=36.0544217687075, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28477; Mature: 28345

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKMTEEFVSYELRGDIALVGLNRPQKRNAISDRFVEAIAAAAARAENEARAAVIFGHG
CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
DHFCAGLDLGEHIHKTPIEGVRGSRRWHAVFSQIEHGTIPWVSALHGAVVGGGLELAAST
CCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCC
HLRVADESAFFALPEGQRGIFVGGGGSVRIARLLGVARMSDMMLTGRVASSAEAERWNLV
EEEEECCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHCEE
QYAVPRGEALAKAQELATIAASNAPLSNYAVINALPRIQDMAKEDGLFVESFISSFTAAS
EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PEAEERLRAFLEKRATRLKAPDTTA
CCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEKMTEEFVSYELRGDIALVGLNRPQKRNAISDRFVEAIAAAAARAENEARAAVIFGHG
CHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
DHFCAGLDLGEHIHKTPIEGVRGSRRWHAVFSQIEHGTIPWVSALHGAVVGGGLELAAST
CCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCC
HLRVADESAFFALPEGQRGIFVGGGGSVRIARLLGVARMSDMMLTGRVASSAEAERWNLV
EEEEECCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHCEE
QYAVPRGEALAKAQELATIAASNAPLSNYAVINALPRIQDMAKEDGLFVESFISSFTAAS
EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PEAEERLRAFLEKRATRLKAPDTTA
CCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA