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The map label for this gene is lexA

Identifier: 15888721

GI number: 15888721

Start: 1390170

End: 1390892

Strand: Reverse

Name: lexA

Synonym: Atu1395

Alternate gene names: 15888721

Gene position: 1390892-1390170 (Counterclockwise)

Preceding gene: 15888722

Following gene: 159184743

Centisome position: 48.95

GC content: 59.2

Gene sequence:

>723_bases
ATGCTCACGCGCAAACAGCAGGAACTGCTTCTCTTCATTCATGAAAGAATGAAAGAGTCCGGCGTGCCGCCCTCCTTCGA
TGAAATGAAGGACGCACTCGATCTTGCTTCCAAATCCGGCATCCACCGGCTGATCACCGCGCTCGAAGAGCGCGGGTTTA
TTCGCCGGCTACCTAACAGGGCGCGAGCGCTGGAGGTTATCAAACTGCCCGAGGCTTATACGCCGGGCGCAAGGCCGCAA
CGCGGCTTTTCGCCGAGCGTCATCGAGGGCAGCCTTGGAAAACCGAAGGAGCCGGAACCGGCGCCTGCCGTGAAGGCGCC
TGCCAATGATTTCGCCGGAGCTGCCACCATTCCCGTCATGGGTCGTATCGCTGCCGGTGTGCCGATTTCCGCCATCCAGA
ACAATACCCATGATTTGGCCGTGCCGGTGGACATGCTGGGTTCGGGCGAGCACTACGCGCTTGAGGTCAAAGGCGATTCG
ATGATCGAGGCCGGGATTTTTGATGGCGATACCGTCATCATCCGCAACGGTAATACCGCCAATCCGGGCGATATCGTCGT
GGCGCTGGTGGATGATGAAGAAGCCACGCTGAAGCGCTTCCGCCGCAAGGGTGCGTCCATCGCGTTGGAAGCGGCCAATC
CTGCCTACGAGACCCGCATTTTTGGACCGGATCGGGTGAAGATTCAGGGCAAGCTGGTCGGGTTGATACGCCGTTACCAT
TGA

Upstream 100 bases:

>100_bases
CACGAAATTTTAGGGGATTGCGGAACACATATGGAACATATAGTGTTCGTTCTGTATTTGTTTCGCGACTTCAACGACGA
TTCTCAAGGGTGTTTTCCTC

Downstream 100 bases:

>100_bases
TAGCTGGAGGCTGCTGCCGGTATTACTGAAACCGAATAGGTTTTCACGGGTAGATTTGGCTTTCGAAATGAATGGCGCAT
CGTTCCGTTGAATTGATGCG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYTPGARPQ
RGFSPSVIEGSLGKPKEPEPAPAVKAPANDFAGAATIPVMGRIAAGVPISAIQNNTHDLAVPVDMLGSGEHYALEVKGDS
MIEAGIFDGDTVIIRNGNTANPGDIVVALVDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH

Sequences:

>Translated_240_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYTPGARPQ
RGFSPSVIEGSLGKPKEPEPAPAVKAPANDFAGAATIPVMGRIAAGVPISAIQNNTHDLAVPVDMLGSGEHYALEVKGDS
MIEAGIFDGDTVIIRNGNTANPGDIVVALVDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
>Mature_240_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYTPGARPQ
RGFSPSVIEGSLGKPKEPEPAPAVKAPANDFAGAATIPVMGRIAAGVPISAIQNNTHDLAVPVDMLGSGEHYALEVKGDS
MIEAGIFDGDTVIIRNGNTANPGDIVVALVDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=238, Percent_Identity=24.7899159663866, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_AGRT5 (Q8UFK2)

Other databases:

- EMBL:   AE007869
- PIR:   AC2748
- PIR:   B97529
- RefSeq:   NP_354402.1
- ProteinModelPortal:   Q8UFK2
- SMR:   Q8UFK2
- STRING:   Q8UFK2
- MEROPS:   S24.001
- GeneID:   1133433
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1395
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- ProtClustDB:   PRK00215
- BioCyc:   ATUM176299-1:ATU1395-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 26033; Mature: 26033

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: ACT_SITE 160-160 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVIKLPEAYTPGARPQRGFSPSVIEGSLGKPKEPEPAPAVKAPANDFAGAATIPVM
HHHEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
GRIAAGVPISAIQNNTHDLAVPVDMLGSGEHYALEVKGDSMIEAGIFDGDTVIIRNGNTA
HHHHCCCCHHHHCCCCCCEEEEHHHCCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCC
NPGDIVVALVDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
CCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVIKLPEAYTPGARPQRGFSPSVIEGSLGKPKEPEPAPAVKAPANDFAGAATIPVM
HHHEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
GRIAAGVPISAIQNNTHDLAVPVDMLGSGEHYALEVKGDSMIEAGIFDGDTVIIRNGNTA
HHHHCCCCHHHHCCCCCCEEEEHHHCCCCCEEEEEECCCCEEEECCCCCCEEEEECCCCC
NPGDIVVALVDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
CCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194