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The map label for this gene is prfB [H]

Identifier: 15888668

GI number: 15888668

Start: 1331605

End: 1332633

Strand: Direct

Name: prfB [H]

Synonym: Atu1342

Alternate gene names: 15888668

Gene position: 1331605-1332633 (Clockwise)

Preceding gene: 15888667

Following gene: 159184721

Centisome position: 46.86

GC content: 60.64

Gene sequence:

>1029_bases
TTGAACAATAAGGCAGAGGACCCGACCCTCTGGAACGATGCCTCCGAAGCCCAGAAGCTGATGCGCGAACGCCAGCAGCT
GGATGACAGCATCAACGGCGTCAAGGCGCTGGAGCAGCAGCTCAAGGACAATATCGAGCTGATCGAACTCGGCGAGATGG
AAGGCGACGATGAGATCGTCAAGGACGCCGAAGATGCGCTGAAGGCGCTGAGAAGCGAAGCGAACCGCCGCCAGGTGGAA
GCCATGCTTTCCGGCGAGGCCGACAGCAACGACACCTATGTGGAAGTGCATTCCGGCGCCGGCGGCACCGAAAGCCAGGA
TTGGGCGAACATGCTGCTGCGCATGTACACCCGCTGGGCGGAGCGTCAGGGCTTCAAGGTCGAAGTTCTCGAAATCCACG
ATGGCGATGAGGCGGGCATCAAGTCGGCCACCATTCTGGTCAAGGGCCATAACGCCTTTGGCTGGATGAAGACGGAATCA
GGCGTGCACCGCCTCGTTCGCATCTCGCCCTATGACAGCAATGCCCGGCGTCACACCTCGTTTTCTTCCATCTGGGTCTA
TCCGGTGGTCGATGATTCGATCCAGATCGACATCAATGAAAGCGATTGCCGTATCGATACCTATCGCTCGTCCGGTGCGG
GCGGCCAGCACGTCAACACCACTGACTCGGCTGTGCGTATCACGCATATTCCGACCGGCATCGCCGTTGCCTGCCAGCAG
GAGCGCTCCCAGCACAAGAACCGCGCCAAGGCGTGGGACATGCTGCGCGCCCGCCTCTATGAGGTGGAACTGCAAAAGCG
GGAAGAGGCGGCCAATGCGCAGGCCGCATCCAAGACCGATATCGGCTGGGGCCATCAGATCCGTTCCTACGTTCTCCAGC
CCTACCAGCTGGTCAAGGACCTGCGCACCGGCGTGGAAAGCACCGCGCCCGGCAACGTGCTGGATGGTGACCTGAACGAG
TTCATGGAAGCCGCACTTGCCCATCGCATCAGCGGCGGCGCGGATGTGGAGGTCTCGGATATCGATTAA

Upstream 100 bases:

>100_bases
CGCAATGAAATCGTGAACGTAGTCGATGAAATCAAGCAGGCCATAAGCCTGCTGAGGAGGCATCTTTGACTGGGATCAGG
CGATAAGACGACTGGACTGG

Downstream 100 bases:

>100_bases
GACGCCCCGGCGCGTCGATCGGAAATTTGCCCCTCGCAAACCCGTTGCGAGGGGTTTTTCGTTGGAGGATACACATGAAA
CAGGCGCTTTATATCGTCGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIVKDAEDALKALRSEANRRQVE
AMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTAPGNVLDGDLNE
FMEAALAHRISGGADVEVSDID

Sequences:

>Translated_342_residues
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIVKDAEDALKALRSEANRRQVE
AMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTAPGNVLDGDLNE
FMEAALAHRISGGADVEVSDID
>Mature_342_residues
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIVKDAEDALKALRSEANRRQVE
AMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTAPGNVLDGDLNE
FMEAALAHRISGGADVEVSDID

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=319, Percent_Identity=36.0501567398119, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI34577120, Length=253, Percent_Identity=35.5731225296443, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI166795305, Length=223, Percent_Identity=34.9775784753363, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=52.760736196319, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787462, Length=322, Percent_Identity=36.9565217391304, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=247, Percent_Identity=34.412955465587, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=304, Percent_Identity=34.8684210526316, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=44.3877551020408, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38154; Mature: 38154

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KDAEDALKALRSEANRRQVEAMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
ERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTESGVHRLVRISPYDSNARRHTS
HCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCEEEEEECCCCCCCCCCCC
FSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
CCEEEEEEEECCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
LRTGVESTAPGNVLDGDLNEFMEAALAHRISGGADVEVSDID
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KDAEDALKALRSEANRRQVEAMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
ERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTESGVHRLVRISPYDSNARRHTS
HCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCEEEEEECCCCCCCCCCCC
FSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
CCEEEEEEEECCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
LRTGVESTAPGNVLDGDLNEFMEAALAHRISGGADVEVSDID
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA