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The map label for this gene is aat

Identifier: 15888654

GI number: 15888654

Start: 1316226

End: 1316840

Strand: Reverse

Name: aat

Synonym: Atu1329

Alternate gene names: 15888654

Gene position: 1316840-1316226 (Counterclockwise)

Preceding gene: 15888655

Following gene: 159184712

Centisome position: 46.34

GC content: 58.54

Gene sequence:

>615_bases
ATGGCTGGGCGGCGCAGCAGAAACAACGACATAACGGTCGATATTCTGCTGCGCGCCTATTCCGCCGGCCTGTTTCCCAT
GGCCGATTCGGCTGATGATCCCGAGCTTTTCTGGGTCGAGCCGGAGATACGCGGTATCATTCCGCTCGATGATTTCCACG
TTTCCAAAAGCCTCGCCAAGGCGATGCGCAAGAAGCCGTTTGCGATACGCTTCAACACCGCTTTCGAAGATGTCATGGCG
GGCTGTGCGGCGGAAGCGGCGGACAGGCCAAGCACCTGGATCAACGCCACCATCCGCCGGCTCTACACCGAGCTGCACCA
GATCGGTCATGCCCACAGCGTTGAGGCATGGGAGGGTGATGAGCTTGTCGGCGGGCTTTACGGTGTTTCTCTGGGTGCTG
CCTTCTTCGGCGAAAGCATGTTTTCCCGCCGCACCAATGCGTCGAAGATCTGCCTCGTCCATCTGGTGGAGCGATTAAAC
GCGAAGGGCTTCGTGCTGCTCGACACGCAATTCACCACAGAACATCTGAAGACGTTCGGCGCGATAGACGTGCCCAAGCT
GGACTATGCAAGAATGCTCGATCTCGCCGTCAACCGGCCGAGCCTGCAATTCTAG

Upstream 100 bases:

>100_bases
TTTCCAGGATCTCCTTCAGAACGAGGATATTCTGAACGGCGATTACGACATTCACTGGCTGGAGAAGTATCTGGCCGGTG
ACGCGTCTCACTAAGACGAT

Downstream 100 bases:

>100_bases
TCTTTACGTTAGGTTATTTGCTTGCCGTTGAGGGAGCTGGAACCTCGGAGTCCTGCTTGCAACCCGTCAGCCACACGTCA
TAGATCGGGTGCTCCACGGC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MAGRRSRNNDITVDILLRAYSAGLFPMADSADDPELFWVEPEIRGIIPLDDFHVSKSLAKAMRKKPFAIRFNTAFEDVMA
GCAAEAADRPSTWINATIRRLYTELHQIGHAHSVEAWEGDELVGGLYGVSLGAAFFGESMFSRRTNASKICLVHLVERLN
AKGFVLLDTQFTTEHLKTFGAIDVPKLDYARMLDLAVNRPSLQF

Sequences:

>Translated_204_residues
MAGRRSRNNDITVDILLRAYSAGLFPMADSADDPELFWVEPEIRGIIPLDDFHVSKSLAKAMRKKPFAIRFNTAFEDVMA
GCAAEAADRPSTWINATIRRLYTELHQIGHAHSVEAWEGDELVGGLYGVSLGAAFFGESMFSRRTNASKICLVHLVERLN
AKGFVLLDTQFTTEHLKTFGAIDVPKLDYARMLDLAVNRPSLQF
>Mature_203_residues
AGRRSRNNDITVDILLRAYSAGLFPMADSADDPELFWVEPEIRGIIPLDDFHVSKSLAKAMRKKPFAIRFNTAFEDVMAG
CAAEAADRPSTWINATIRRLYTELHQIGHAHSVEAWEGDELVGGLYGVSLGAAFFGESMFSRRTNASKICLVHLVERLNA
KGFVLLDTQFTTEHLKTFGAIDVPKLDYARMLDLAVNRPSLQF

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=187, Percent_Identity=39.572192513369, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_AGRT5 (Q8UFR8)

Other databases:

- EMBL:   AE007869
- PIR:   AI2739
- PIR:   G97520
- RefSeq:   NP_354335.1
- ProteinModelPortal:   Q8UFR8
- SMR:   Q8UFR8
- STRING:   Q8UFR8
- GeneID:   1133367
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1329
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q8UFR8
- ProtClustDB:   PRK00301
- BioCyc:   ATUM176299-1:ATU1329-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 22762; Mature: 22631

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRRSRNNDITVDILLRAYSAGLFPMADSADDPELFWVEPEIRGIIPLDDFHVSKSLAK
CCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEECCCCHHHHHHHHHH
AMRKKPFAIRFNTAFEDVMAGCAAEAADRPSTWINATIRRLYTELHQIGHAHSVEAWEGD
HHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ELVGGLYGVSLGAAFFGESMFSRRTNASKICLVHLVERLNAKGFVLLDTQFTTEHLKTFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHC
AIDVPKLDYARMLDLAVNRPSLQF
CCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGRRSRNNDITVDILLRAYSAGLFPMADSADDPELFWVEPEIRGIIPLDDFHVSKSLAK
CCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEECCCCHHHHHHHHHH
AMRKKPFAIRFNTAFEDVMAGCAAEAADRPSTWINATIRRLYTELHQIGHAHSVEAWEGD
HHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ELVGGLYGVSLGAAFFGESMFSRRTNASKICLVHLVERLNAKGFVLLDTQFTTEHLKTFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHC
AIDVPKLDYARMLDLAVNRPSLQF
CCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194