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The map label for this gene is cpdB [H]

Identifier: 15888546

GI number: 15888546

Start: 1203727

End: 1205715

Strand: Reverse

Name: cpdB [H]

Synonym: Atu1210

Alternate gene names: 15888546

Gene position: 1205715-1203727 (Counterclockwise)

Preceding gene: 159185432

Following gene: 159184667

Centisome position: 42.43

GC content: 59.63

Gene sequence:

>1989_bases
ATGTCGTCGCTTCTCGCTCTCCACCCCATCACCCGCCGTTCGCTTCTCGGCGGCATTGCGGCCTCTTCGGCACTGGTGTT
GCTGCATCCCTTTGCCGCACGCGCAGCGGCCAATCAGGCGCATCTGCGCATCATGGAAACGACTGACATCCATGTTCACG
TCTTTCCCTATGATTATTACGCCGACAAGCCGAACGACACGATGGGACTTTCGCGCACCGCCTCGATCATCGACAAGATC
CGCGCCGAGGCCGGAAACTCGATGCTGGTGGATAATGGCGACTTTCTGCAGGGCAACCCGCTCGGCGACTATATCGCCTA
TGAGCGCGGCATGAAGGCCGGCGACAAGCATCCGGTCATCAACGCCATGAACGTGCTGGGCTATGATTGCGGCACGCTTG
GCAACCACGAATTCAATTACGGTCTCGACTATATGTCGAACACGCTGGCGGGCGCCGGTTTCCCCTATGTCTGCGCCAAC
CTCACCAAGGGCCAGCTCGCTTCCGATCCAAAAAACGACGAGCTGTTCTTCAAGCCCTATGTCATTCTGGAAAAGCAGAT
CCGCGATGGCGGCGGCGTGACGAGCCCGGTCAAGGTCGGCATCATTGGCTTCGTGCCGCCGCAGATCATGATGTGGGATT
CCAAGAACCTCGAGGGCAAGGCCCAGACGCGCGACATCGTCGAGGCGGCGAAAGCCTGGGTGCCTGTCATGAAGGAAGAA
GGCGCCGATATCATCATCGCCCTTTCCCATTCCGGCATCGACGGCAAGGGCCAGACGGAGCGCATGGAAAACGCCTCGCT
TTATCTGGCCGCCGTTGAAGGCATCGATGCCATCTTCACCGGCCACCAGCATCTTGTCTTTCCCGGTCCGAAGACCTGGG
ACGGCATCGAGGGTGCCGATGCGGTCAAGGGCACGCTGATGGGCAAGCCGGCGGTCATGGCCGGTTTCTGGGGCTCGCAT
ATGGGCCTCATCGACCTCTTGCTCGAAAAGGATGGCAAGAGTTGGAAGATCGTCGACTTCACCTCTGAGGCCCGGCCGAT
CTATCACCGCGATGACAGTCGCAAGGTCGTCGCCGATTTCACTGACAAGCCGGAGGTTCTCGCCGCCGCCAAGACTGACC
ACGAGGCAGCGCTTGCCTATGTGCGCCGTCCCGTCGGCAAGACGTCCGCGCCGCTCTACTCCTATTTCGCGCTGGTGGCT
GATGATCCGTCCGTGCAGATCGTCTCCAACGCCCAGACCTGGTACATCAAGGACATGCTGAAGGAAGGGCAATATAAGGA
TCTGCCGGTGCTCTCCGCAGCAGCGCCCTTCAAGTCTGGCGGTCGCGGCGGCGCGGATTATTATACCGATGTCCCCGCCG
GTGACATCGCCATCAAGAACGTCGCCGACCTCTATCTCTATCCCAACACGGTGCAGGCGGTGCTGATCAACGGCGCGCAG
GTGAAGAACTGGCTGGAAATGTCGGCCGGCATGTTCAACACGGTAGAGGCCGGTGCCAAGGACGCGCCGTTGCTCAACGC
CGACTTCCCGTCCTATAATTTCGATGTGATCGACGGCGTCACCTATCAGATCGACATTTCCAAACCGGCGAAATTCGACA
AGGACGGCAAGGCGGTCAATCCCGACAGCAACCGCATCGTGAACCTCCAGTTCAACGGCAAGCCGATCGATCCCGAGCAG
AAATTCGTGGTGGCCACCAATAATTACCGCGCGAGCGGCGGCGGTAAATTCCCCGACATCGCCGGTGACAAGGTGATCTT
CGTAGCGCCCGATACCAACCGCGACGTGATCGTGCGCTATATCATCGATCAGGGCACCATCAATCCGTCCGCGGATGCCA
ACTGGTCCTTTGCACCGGTGGCCAATACCACGGCAATCTTCGAGACCGGCCCCAAAGGCCGCCACTATGCGGCGGAAATC
AAGGGCGCGAAAATCGAGGATGCTGGTGACGGTGCGGAAGGTTTCGCGAAGTTCCGTCTGGTTCTCTAG

Upstream 100 bases:

>100_bases
CCGACTGTCACGGGCTTGTCATTTTGGTGATGGACTCCTCGGCGATTGCTTCTACCCTGTCGTTTTCCTGACAGAGCAAG
CTTCATCCGAAGGAGTTCCC

Downstream 100 bases:

>100_bases
GGAACACGACTCTCCCGGTGATACATCGGCGGGAGAGCACTTCTTCAGAACCGATGCGCCAGACCGACTATCGCCGTGCG
GTGATCGCGCCGAGGCAAGG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSSLLALHPITRRSLLGGIAASSALVLLHPFAARAAANQAHLRIMETTDIHVHVFPYDYYADKPNDTMGLSRTASIIDKI
RAEAGNSMLVDNGDFLQGNPLGDYIAYERGMKAGDKHPVINAMNVLGYDCGTLGNHEFNYGLDYMSNTLAGAGFPYVCAN
LTKGQLASDPKNDELFFKPYVILEKQIRDGGGVTSPVKVGIIGFVPPQIMMWDSKNLEGKAQTRDIVEAAKAWVPVMKEE
GADIIIALSHSGIDGKGQTERMENASLYLAAVEGIDAIFTGHQHLVFPGPKTWDGIEGADAVKGTLMGKPAVMAGFWGSH
MGLIDLLLEKDGKSWKIVDFTSEARPIYHRDDSRKVVADFTDKPEVLAAAKTDHEAALAYVRRPVGKTSAPLYSYFALVA
DDPSVQIVSNAQTWYIKDMLKEGQYKDLPVLSAAAPFKSGGRGGADYYTDVPAGDIAIKNVADLYLYPNTVQAVLINGAQ
VKNWLEMSAGMFNTVEAGAKDAPLLNADFPSYNFDVIDGVTYQIDISKPAKFDKDGKAVNPDSNRIVNLQFNGKPIDPEQ
KFVVATNNYRASGGGKFPDIAGDKVIFVAPDTNRDVIVRYIIDQGTINPSADANWSFAPVANTTAIFETGPKGRHYAAEI
KGAKIEDAGDGAEGFAKFRLVL

Sequences:

>Translated_662_residues
MSSLLALHPITRRSLLGGIAASSALVLLHPFAARAAANQAHLRIMETTDIHVHVFPYDYYADKPNDTMGLSRTASIIDKI
RAEAGNSMLVDNGDFLQGNPLGDYIAYERGMKAGDKHPVINAMNVLGYDCGTLGNHEFNYGLDYMSNTLAGAGFPYVCAN
LTKGQLASDPKNDELFFKPYVILEKQIRDGGGVTSPVKVGIIGFVPPQIMMWDSKNLEGKAQTRDIVEAAKAWVPVMKEE
GADIIIALSHSGIDGKGQTERMENASLYLAAVEGIDAIFTGHQHLVFPGPKTWDGIEGADAVKGTLMGKPAVMAGFWGSH
MGLIDLLLEKDGKSWKIVDFTSEARPIYHRDDSRKVVADFTDKPEVLAAAKTDHEAALAYVRRPVGKTSAPLYSYFALVA
DDPSVQIVSNAQTWYIKDMLKEGQYKDLPVLSAAAPFKSGGRGGADYYTDVPAGDIAIKNVADLYLYPNTVQAVLINGAQ
VKNWLEMSAGMFNTVEAGAKDAPLLNADFPSYNFDVIDGVTYQIDISKPAKFDKDGKAVNPDSNRIVNLQFNGKPIDPEQ
KFVVATNNYRASGGGKFPDIAGDKVIFVAPDTNRDVIVRYIIDQGTINPSADANWSFAPVANTTAIFETGPKGRHYAAEI
KGAKIEDAGDGAEGFAKFRLVL
>Mature_661_residues
SSLLALHPITRRSLLGGIAASSALVLLHPFAARAAANQAHLRIMETTDIHVHVFPYDYYADKPNDTMGLSRTASIIDKIR
AEAGNSMLVDNGDFLQGNPLGDYIAYERGMKAGDKHPVINAMNVLGYDCGTLGNHEFNYGLDYMSNTLAGAGFPYVCANL
TKGQLASDPKNDELFFKPYVILEKQIRDGGGVTSPVKVGIIGFVPPQIMMWDSKNLEGKAQTRDIVEAAKAWVPVMKEEG
ADIIIALSHSGIDGKGQTERMENASLYLAAVEGIDAIFTGHQHLVFPGPKTWDGIEGADAVKGTLMGKPAVMAGFWGSHM
GLIDLLLEKDGKSWKIVDFTSEARPIYHRDDSRKVVADFTDKPEVLAAAKTDHEAALAYVRRPVGKTSAPLYSYFALVAD
DPSVQIVSNAQTWYIKDMLKEGQYKDLPVLSAAAPFKSGGRGGADYYTDVPAGDIAIKNVADLYLYPNTVQAVLINGAQV
KNWLEMSAGMFNTVEAGAKDAPLLNADFPSYNFDVIDGVTYQIDISKPAKFDKDGKAVNPDSNRIVNLQFNGKPIDPEQK
FVVATNNYRASGGGKFPDIAGDKVIFVAPDTNRDVIVRYIIDQGTINPSADANWSFAPVANTTAIFETGPKGRHYAAEIK
GAKIEDAGDGAEGFAKFRLVL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=623, Percent_Identity=47.5120385232745, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1786687, Length=624, Percent_Identity=21.7948717948718, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 71626; Mature: 71495

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLALHPITRRSLLGGIAASSALVLLHPFAARAAANQAHLRIMETTDIHVHVFPYDYY
CCCCEEECCHHHHHHHHHHHHCCEEEEEECHHHHHCCCCEEEEEEEECEEEEEEEEEHHC
ADKPNDTMGLSRTASIIDKIRAEAGNSMLVDNGDFLQGNPLGDYIAYERGMKAGDKHPVI
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCHHHHHHCCCCCCCCCCCH
NAMNVLGYDCGTLGNHEFNYGLDYMSNTLAGAGFPYVCANLTKGQLASDPKNDELFFKPY
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCEEECCE
VILEKQIRDGGGVTSPVKVGIIGFVPPQIMMWDSKNLEGKAQTRDIVEAAKAWVPVMKEE
EEEEHHHCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
GADIIIALSHSGIDGKGQTERMENASLYLAAVEGIDAIFTGHQHLVFPGPKTWDGIEGAD
CCCEEEEEECCCCCCCCCHHHHCCCEEEEEEECCCEEEEECCEEEEECCCCCCCCCCCCH
AVKGTLMGKPAVMAGFWGSHMGLIDLLLEKDGKSWKIVDFTSEARPIYHRDDSRKVVADF
HHHHEECCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCEEEEEC
TDKPEVLAAAKTDHEAALAYVRRPVGKTSAPLYSYFALVADDPSVQIVSNAQTWYIKDML
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEHHHHH
KEGQYKDLPVLSAAAPFKSGGRGGADYYTDVPAGDIAIKNVADLYLYPNTVQAVLINGAQ
HCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCEEEEEECCHH
VKNWLEMSAGMFNTVEAGAKDAPLLNADFPSYNFDVIDGVTYQIDISKPAKFDKDGKAVN
HHHHHHHCCCCCHHHHCCCCCCCEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCCEEC
PDSNRIVNLQFNGKPIDPEQKFVVATNNYRASGGGKFPDIAGDKVIFVAPDTNRDVIVRY
CCCCEEEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEE
IIDQGTINPSADANWSFAPVANTTAIFETGPKGRHYAAEIKGAKIEDAGDGAEGFAKFRL
EEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEECCCEECCCCCCCCCEEEEEE
VL
EC
>Mature Secondary Structure 
SSLLALHPITRRSLLGGIAASSALVLLHPFAARAAANQAHLRIMETTDIHVHVFPYDYY
CCCEEECCHHHHHHHHHHHHCCEEEEEECHHHHHCCCCEEEEEEEECEEEEEEEEEHHC
ADKPNDTMGLSRTASIIDKIRAEAGNSMLVDNGDFLQGNPLGDYIAYERGMKAGDKHPVI
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCHHHHHHCCCCCCCCCCCH
NAMNVLGYDCGTLGNHEFNYGLDYMSNTLAGAGFPYVCANLTKGQLASDPKNDELFFKPY
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCEEECCE
VILEKQIRDGGGVTSPVKVGIIGFVPPQIMMWDSKNLEGKAQTRDIVEAAKAWVPVMKEE
EEEEHHHCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
GADIIIALSHSGIDGKGQTERMENASLYLAAVEGIDAIFTGHQHLVFPGPKTWDGIEGAD
CCCEEEEEECCCCCCCCCHHHHCCCEEEEEEECCCEEEEECCEEEEECCCCCCCCCCCCH
AVKGTLMGKPAVMAGFWGSHMGLIDLLLEKDGKSWKIVDFTSEARPIYHRDDSRKVVADF
HHHHEECCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCEEEEEC
TDKPEVLAAAKTDHEAALAYVRRPVGKTSAPLYSYFALVADDPSVQIVSNAQTWYIKDML
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEHHHHH
KEGQYKDLPVLSAAAPFKSGGRGGADYYTDVPAGDIAIKNVADLYLYPNTVQAVLINGAQ
HCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCEEEEEECCHH
VKNWLEMSAGMFNTVEAGAKDAPLLNADFPSYNFDVIDGVTYQIDISKPAKFDKDGKAVN
HHHHHHHCCCCCHHHHCCCCCCCEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCCEEC
PDSNRIVNLQFNGKPIDPEQKFVVATNNYRASGGGKFPDIAGDKVIFVAPDTNRDVIVRY
CCCCEEEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEE
IIDQGTINPSADANWSFAPVANTTAIFETGPKGRHYAAEIKGAKIEDAGDGAEGFAKFRL
EEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEECCCEECCCCCCCCCEEEEEE
VL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]