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The map label for this gene is cscA [H]

Identifier: 15888283

GI number: 15888283

Start: 931736

End: 933367

Strand: Reverse

Name: cscA [H]

Synonym: Atu0944

Alternate gene names: 15888283

Gene position: 933367-931736 (Counterclockwise)

Preceding gene: 15888285

Following gene: 159184544

Centisome position: 32.85

GC content: 61.58

Gene sequence:

>1632_bases
GTGTGGCTGAAGGCGAAAAAAGACGCCGAAGCCGGCGCTGTCTCTTTCTCCAACCAGAACGGAAAATTCGCCGAAGTTTC
AAGCGTCAATACCGAAGAGTTCAGTTTTCGAATCTATCAGGTCTTTGGCGGCGGCCATGTGGAGCTGAGCTACGACACGG
TGACCACGGCGGTTTCCGTCATCTACTGGTTCACCGCTTCGGATGTGCTGGAAACCGGCATCACCGTCGTCCATACCAAG
CCGGACAATGCCGCACCTGAGCTACCGGGAAGCTATCATTTCCGCCCGCCCTTCGGCTGGATGAACGACCCGAACGGCTT
TGGCCGCTTCGAGGGCAGGCCACATCTGTTCTATCAGCATTATTCGCACGGGCTGCGCTGGAACACCATGCATTGGGGCC
ATGCGGTCTCCTCGGATTATCTGCGCTGGCGGCATCTACCGATCTTCCTTTTCCCCTCCGAGGACCTGACGACGCGGCCG
GACAAACGCGGCGGCGCCTATTCCGGCTCGACCATTCCGCTGGTGGAAGGCTCAGGCATCCGGGTCTTTTTCACCGAGCA
GGTGCAGGACCGTATTCCCGAGCAGCAGATCCAGCTTACCGCCACCTCGTCTGATCTCATCATGGCCGGGCAGGCAGACG
TCATTCTCGCCCACCGCCCGGATGGGCAGGGGCTGACGCCTGACTTCCGCGACCCTTACGTGTTTCGTGGCCCCGATGGC
TTGTGGAAAATGCTGCTCGGCAGCCAGAGCGATGGCGGCGGCGTGATCCTGCTTTACGAGACGCTGGACCCCACGGCCGC
CTCCGGCTGGACCTATGTGGGCAAGCTGTGGGTGGAGACACGTTACAAGACCACGGCCATCGAATGCCCCTGCCTGCTGC
CCCTCGATGGCCCCGCCAATGCCCGTTCCACAAGGTGGGTGCTGATCTACGGGCTGATGCATTCGGAAGACCCGGAGACC
GGCCGCAAGAACCTGACCATGGCCGATGTCGGCTGGTTTGACGGCAAGGTCTTCACCAAGGAATTTGGTCAGGAGCTGGA
TTTCGGCACCGATAATTACGCCTTTCAGGCCTTTCTGGATGGCGACAGCATTATCGGCATCGGCTGGCTGGCAAACTGGG
CGGATGCCAATCCGGAAATCGATTTCCCCACCTCCATGACCCTGCCGCGCCGAATTCACTACGCGAACGGCGAACTTCTG
ACCCCGCCGATCGGCGCGGCGGAAAGCCTGCGCAGCCATATTCTCGACCGCACGCGGCTGGCGGCCGGTGAGCGCGTCAC
CTTCATCAACGGCGCGGTGGAAATATTGATCGAACTTGCCTCGTCAGACGCGGCCTTCGAGCTGGCGCTCGATCATCCGA
CCGTCACGCTCGGTCTCGTCTCCGACGAGACGGGCCTGTGGATCCGCCATGAGGACGGGCGCGACGGTGCTTCGCCGCAC
TACATTGCCAAGGGCGCGCGCGCATCCCGCATCCGCATTTTCCTCGACTACGGCTCCATCGAGGTCTTTGCAAATCGCGG
CCGTTATGTCGGTACCAAGCGCATCGAAGGTTTCGAACCGGTGCGTTCGGCGCTGCTCCGCGCCGACCCCGGTGCGGTGG
TGCATGCCACCAGTTGGGCACTGAAACCGTGA

Upstream 100 bases:

>100_bases
GAAAGGAACGGGAATGACCGTCGTTGAAGCCGTAACTGCAGAACCGGAAAGCGCCATCCGCACCCTTCACACCGACCTGC
CGGTCAACGCCACCTTCCAT

Downstream 100 bases:

>100_bases
GGAACCTTTTGGCGGACTGGGCGGTTGATAGACCGAAACCGCCCAATCCACTGATAAGGAGAAACGGCATGACCAAAATC
CGCGATACCAGCGACCCGAT

Product: sucrose hydrolase

Products: NA

Alternate protein names: Invertase; Sucrase [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MWLKAKKDAEAGAVSFSNQNGKFAEVSSVNTEEFSFRIYQVFGGGHVELSYDTVTTAVSVIYWFTASDVLETGITVVHTK
PDNAAPELPGSYHFRPPFGWMNDPNGFGRFEGRPHLFYQHYSHGLRWNTMHWGHAVSSDYLRWRHLPIFLFPSEDLTTRP
DKRGGAYSGSTIPLVEGSGIRVFFTEQVQDRIPEQQIQLTATSSDLIMAGQADVILAHRPDGQGLTPDFRDPYVFRGPDG
LWKMLLGSQSDGGGVILLYETLDPTAASGWTYVGKLWVETRYKTTAIECPCLLPLDGPANARSTRWVLIYGLMHSEDPET
GRKNLTMADVGWFDGKVFTKEFGQELDFGTDNYAFQAFLDGDSIIGIGWLANWADANPEIDFPTSMTLPRRIHYANGELL
TPPIGAAESLRSHILDRTRLAAGERVTFINGAVEILIELASSDAAFELALDHPTVTLGLVSDETGLWIRHEDGRDGASPH
YIAKGARASRIRIFLDYGSIEVFANRGRYVGTKRIEGFEPVRSALLRADPGAVVHATSWALKP

Sequences:

>Translated_543_residues
MWLKAKKDAEAGAVSFSNQNGKFAEVSSVNTEEFSFRIYQVFGGGHVELSYDTVTTAVSVIYWFTASDVLETGITVVHTK
PDNAAPELPGSYHFRPPFGWMNDPNGFGRFEGRPHLFYQHYSHGLRWNTMHWGHAVSSDYLRWRHLPIFLFPSEDLTTRP
DKRGGAYSGSTIPLVEGSGIRVFFTEQVQDRIPEQQIQLTATSSDLIMAGQADVILAHRPDGQGLTPDFRDPYVFRGPDG
LWKMLLGSQSDGGGVILLYETLDPTAASGWTYVGKLWVETRYKTTAIECPCLLPLDGPANARSTRWVLIYGLMHSEDPET
GRKNLTMADVGWFDGKVFTKEFGQELDFGTDNYAFQAFLDGDSIIGIGWLANWADANPEIDFPTSMTLPRRIHYANGELL
TPPIGAAESLRSHILDRTRLAAGERVTFINGAVEILIELASSDAAFELALDHPTVTLGLVSDETGLWIRHEDGRDGASPH
YIAKGARASRIRIFLDYGSIEVFANRGRYVGTKRIEGFEPVRSALLRADPGAVVHATSWALKP
>Mature_543_residues
MWLKAKKDAEAGAVSFSNQNGKFAEVSSVNTEEFSFRIYQVFGGGHVELSYDTVTTAVSVIYWFTASDVLETGITVVHTK
PDNAAPELPGSYHFRPPFGWMNDPNGFGRFEGRPHLFYQHYSHGLRWNTMHWGHAVSSDYLRWRHLPIFLFPSEDLTTRP
DKRGGAYSGSTIPLVEGSGIRVFFTEQVQDRIPEQQIQLTATSSDLIMAGQADVILAHRPDGQGLTPDFRDPYVFRGPDG
LWKMLLGSQSDGGGVILLYETLDPTAASGWTYVGKLWVETRYKTTAIECPCLLPLDGPANARSTRWVLIYGLMHSEDPET
GRKNLTMADVGWFDGKVFTKEFGQELDFGTDNYAFQAFLDGDSIIGIGWLANWADANPEIDFPTSMTLPRRIHYANGELL
TPPIGAAESLRSHILDRTRLAAGERVTFINGAVEILIELASSDAAFELALDHPTVTLGLVSDETGLWIRHEDGRDGASPH
YIAKGARASRIRIFLDYGSIEVFANRGRYVGTKRIEGFEPVRSALLRADPGAVVHATSWALKP

Specific function: Hydrolysis of sucrose, raffinose, inulin and levan. Specific for the fructose moiety and the beta-anomeric configuration of the glycosidic linkages of its substrates. The enzyme released fructose from sucrose and raffinose, and the fructose polymer inulin

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=463, Percent_Identity=26.3498920086393, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 60223; Mature: 60223

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLKAKKDAEAGAVSFSNQNGKFAEVSSVNTEEFSFRIYQVFGGGHVELSYDTVTTAVSV
CCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEHHHHHHHEEE
IYWFTASDVLETGITVVHTKPDNAAPELPGSYHFRPPFGWMNDPNGFGRFEGRPHLFYQH
HEEECHHHHHHCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEHH
YSHGLRWNTMHWGHAVSSDYLRWRHLPIFLFPSEDLTTRPDKRGGAYSGSTIPLVEGSGI
HCCCCEECEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCE
RVFFTEQVQDRIPEQQIQLTATSSDLIMAGQADVILAHRPDGQGLTPDFRDPYVFRGPDG
EEEEEHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCH
LWKMLLGSQSDGGGVILLYETLDPTAASGWTYVGKLWVETRYKTTAIECPCLLPLDGPAN
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEEEEEECCEEEECCCCCC
ARSTRWVLIYGLMHSEDPETGRKNLTMADVGWFDGKVFTKEFGQELDFGTDNYAFQAFLD
CCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEHHHHCCCCCCCCCCEEEEEEEC
GDSIIGIGWLANWADANPEIDFPTSMTLPRRIHYANGELLTPPIGAAESLRSHILDRTRL
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHH
AAGERVTFINGAVEILIELASSDAAFELALDHPTVTLGLVSDETGLWIRHEDGRDGASPH
CCCCEEEEECHHHHEEEECCCCCCEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCC
YIAKGARASRIRIFLDYGSIEVFANRGRYVGTKRIEGFEPVRSALLRADPGAVVHATSWA
EEECCCCCEEEEEEEEECCEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCEEEEECEE
LKP
CCC
>Mature Secondary Structure
MWLKAKKDAEAGAVSFSNQNGKFAEVSSVNTEEFSFRIYQVFGGGHVELSYDTVTTAVSV
CCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEHHHHHHHEEE
IYWFTASDVLETGITVVHTKPDNAAPELPGSYHFRPPFGWMNDPNGFGRFEGRPHLFYQH
HEEECHHHHHHCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEHH
YSHGLRWNTMHWGHAVSSDYLRWRHLPIFLFPSEDLTTRPDKRGGAYSGSTIPLVEGSGI
HCCCCEECEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCE
RVFFTEQVQDRIPEQQIQLTATSSDLIMAGQADVILAHRPDGQGLTPDFRDPYVFRGPDG
EEEEEHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCH
LWKMLLGSQSDGGGVILLYETLDPTAASGWTYVGKLWVETRYKTTAIECPCLLPLDGPAN
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEEEEEECCEEEECCCCCC
ARSTRWVLIYGLMHSEDPETGRKNLTMADVGWFDGKVFTKEFGQELDFGTDNYAFQAFLD
CCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEHHHHCCCCCCCCCCEEEEEEEC
GDSIIGIGWLANWADANPEIDFPTSMTLPRRIHYANGELLTPPIGAAESLRSHILDRTRL
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHH
AAGERVTFINGAVEILIELASSDAAFELALDHPTVTLGLVSDETGLWIRHEDGRDGASPH
CCCCEEEEECHHHHEEEECCCCCCEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCC
YIAKGARASRIRIFLDYGSIEVFANRGRYVGTKRIEGFEPVRSALLRADPGAVVHATSWA
EEECCCCCEEEEEEEEECCEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCEEEEECEE
LKP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9720201; 10360571 [H]