Click here to switch to the map view.

The map label for this gene is ygcA [C]

Identifier: 15888078

GI number: 15888078

Start: 734323

End: 735573

Strand: Reverse

Name: ygcA [C]

Synonym: Atu0735

Alternate gene names: 15888078

Gene position: 735573-734323 (Counterclockwise)

Preceding gene: 15888079

Following gene: 159184443

Centisome position: 25.89

GC content: 62.51

Gene sequence:

>1251_bases
ATGAGCACACAGACCGTCACCATCAAGAGCCTTGGCGCGCAGGGCGATGGCATCGCACACTGTCCCGACGGCCCGGTCTA
TGTGCCTTTCGCGCTGCCCGGCGAAACGGTCGCGATTGCCAAGGTCAAGGATCAGGGCACGGTCATGTCGATAACGGAAG
CTTCGGCTGACCGCCGTGACCCCGTCTGTCGCCACTTCGGTCCCGAAGGCATCAACGGCACCTGCGGCGGCTGTTCGTTG
CAGCATCTGGCTGATCAACCCTACCACGCCTTCAAGCGGGAACTGGTGGTTTCGGCACTGAGATCGAAGGGCCTGACCCC
GCCAGTGGACGATCTCGTCATCTGTCGCCCCGGCGAGCGCCGCCGTGCGGTGTTTGCCGCCCGCAAGACGGAAAAGGGTC
TGCTGCTAGGCTTCAGCCAGGCGAACAGCCATCACATCGTCGCCATCGAGGAATGTCCCGTTACCTCGCCGGGCATTGTC
TCACGGTTCGATGCCATCCGCGCCATCGGGCTTTCCATGGTGGCGAATGCCGAGCCGTTCCGCATCACCGTTCTCGAAAC
GCTCTCCGGTCTCGATATTTCCGTCGAGGGCATCAAGTCGGTCAACGACAAGCAGCGGCGCACGCTCACGGAAACGGTGC
TGGCCATGCGCGGCATTGCCCGCGTTTCGCTGTCGGGTGAAATCCTGATCGAACCGCAAAAGCCGATCATCGAATTCGGC
GGCATTCCGGTTTCGCCGCCGGCCGGCGGTTTCACGCAGGCGACGAAGCAGGCCGAAGATGCCATGGCGGAGCTGATGCT
TGCCCATGTCGGCAAATCCAAACGCATTGCCGATCTGTTCTGCGGTTCCGGAACGTTTGCACTCAGGCTCGCGCGCATCG
GCAGGGTGCATGCGGTGGAGGCCGAAGACAAAGCGCTGAAGGCACTGGATTTCGCCGCCCGCAACACGCAGGGCCTGAAA
CCGGTCAGCGTCGAAAAGCGCGATCTCTTCCGCCGGCCGCTGATGACAAGCGAACTCAAGAATTACGATGCCGTCGTGTT
CGATCCGCCGCGCGCCGGTGCGGAAGTCCAGTGCAAGGAACTTGCCCGCAGCACGGTGAAGAAGATCGTTGCGGTGAGCT
GCAATCCGCTGACACTCGCACGCGATCTCGCCATCCTCACGGAAGGCGGCTACCGCGTCACCCGCGTCACGCCGGTCGAC
CAGTTCCTGTGGTCGCCGCATGTGGAAGCAGTGGCGGTACTGGAGAAATAA

Upstream 100 bases:

>100_bases
GGCTGACCGAGGACATGGGCTGGAAAAGCCTCGGCCTCATTCCCTCGCCAATTTCCGGCGGTGACGGCAATGTCGAATTT
CTTCTCGGAGGCGAAAAGCC

Downstream 100 bases:

>100_bases
GCAGTCTGGACGCTGGGTCGTTCACGCGAAGCTGACGCAACGGTTCATAGGCCCGCAAGCCCAAAGGCCCTCATTCCTGT
GCTTGTCACGGGAATCCAGT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSTQTVTIKSLGAQGDGIAHCPDGPVYVPFALPGETVAIAKVKDQGTVMSITEASADRRDPVCRHFGPEGINGTCGGCSL
QHLADQPYHAFKRELVVSALRSKGLTPPVDDLVICRPGERRRAVFAARKTEKGLLLGFSQANSHHIVAIEECPVTSPGIV
SRFDAIRAIGLSMVANAEPFRITVLETLSGLDISVEGIKSVNDKQRRTLTETVLAMRGIARVSLSGEILIEPQKPIIEFG
GIPVSPPAGGFTQATKQAEDAMAELMLAHVGKSKRIADLFCGSGTFALRLARIGRVHAVEAEDKALKALDFAARNTQGLK
PVSVEKRDLFRRPLMTSELKNYDAVVFDPPRAGAEVQCKELARSTVKKIVAVSCNPLTLARDLAILTEGGYRVTRVTPVD
QFLWSPHVEAVAVLEK

Sequences:

>Translated_416_residues
MSTQTVTIKSLGAQGDGIAHCPDGPVYVPFALPGETVAIAKVKDQGTVMSITEASADRRDPVCRHFGPEGINGTCGGCSL
QHLADQPYHAFKRELVVSALRSKGLTPPVDDLVICRPGERRRAVFAARKTEKGLLLGFSQANSHHIVAIEECPVTSPGIV
SRFDAIRAIGLSMVANAEPFRITVLETLSGLDISVEGIKSVNDKQRRTLTETVLAMRGIARVSLSGEILIEPQKPIIEFG
GIPVSPPAGGFTQATKQAEDAMAELMLAHVGKSKRIADLFCGSGTFALRLARIGRVHAVEAEDKALKALDFAARNTQGLK
PVSVEKRDLFRRPLMTSELKNYDAVVFDPPRAGAEVQCKELARSTVKKIVAVSCNPLTLARDLAILTEGGYRVTRVTPVD
QFLWSPHVEAVAVLEK
>Mature_415_residues
STQTVTIKSLGAQGDGIAHCPDGPVYVPFALPGETVAIAKVKDQGTVMSITEASADRRDPVCRHFGPEGINGTCGGCSLQ
HLADQPYHAFKRELVVSALRSKGLTPPVDDLVICRPGERRRAVFAARKTEKGLLLGFSQANSHHIVAIEECPVTSPGIVS
RFDAIRAIGLSMVANAEPFRITVLETLSGLDISVEGIKSVNDKQRRTLTETVLAMRGIARVSLSGEILIEPQKPIIEFGG
IPVSPPAGGFTQATKQAEDAMAELMLAHVGKSKRIADLFCGSGTFALRLARIGRVHAVEAEDKALKALDFAARNTQGLKP
VSVEKRDLFRRPLMTSELKNYDAVVFDPPRAGAEVQCKELARSTVKKIVAVSCNPLTLARDLAILTEGGYRVTRVTPVDQ
FLWSPHVEAVAVLEK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789148, Length=427, Percent_Identity=26.9320843091335, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790403, Length=369, Percent_Identity=25.2032520325203, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y735_AGRT5 (Q8UHE7)

Other databases:

- EMBL:   AE007869
- PIR:   AI2666
- PIR:   G97448
- RefSeq:   NP_353759.1
- ProteinModelPortal:   Q8UHE7
- SMR:   Q8UHE7
- STRING:   Q8UHE7
- GeneID:   1132773
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0735
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   IMSIATP
- PhylomeDB:   Q8UHE7
- ProtClustDB:   CLSK2484339
- BioCyc:   ATUM176299-1:ATU0735-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR002792
- InterPro:   IPR010280

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 44757; Mature: 44626

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 374-374 BINDING 253-253 BINDING 280-280 BINDING 300-300 BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQTVTIKSLGAQGDGIAHCPDGPVYVPFALPGETVAIAKVKDQGTVMSITEASADRRD
CCCCEEEEEECCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
PVCRHFGPEGINGTCGGCSLQHLADQPYHAFKRELVVSALRSKGLTPPVDDLVICRPGER
CHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
RRAVFAARKTEKGLLLGFSQANSHHIVAIEECPVTSPGIVSRFDAIRAIGLSMVANAEPF
HHHEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RITVLETLSGLDISVEGIKSVNDKQRRTLTETVLAMRGIARVSLSGEILIEPQKPIIEFG
EEEEEHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCEEC
GIPVSPPAGGFTQATKQAEDAMAELMLAHVGKSKRIADLFCGSGTFALRLARIGRVHAVE
CEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCHHHHHHHHHCCEEEEC
AEDKALKALDFAARNTQGLKPVSVEKRDLFRRPLMTSELKNYDAVVFDPPRAGAEVQCKE
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCHHHH
LARSTVKKIVAVSCNPLTLARDLAILTEGGYRVTRVTPVDQFLWSPHVEAVAVLEK
HHHHHHHHHHHCCCCCCHHHHHEEEEECCCEEEEEECCHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
STQTVTIKSLGAQGDGIAHCPDGPVYVPFALPGETVAIAKVKDQGTVMSITEASADRRD
CCCEEEEEECCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
PVCRHFGPEGINGTCGGCSLQHLADQPYHAFKRELVVSALRSKGLTPPVDDLVICRPGER
CHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
RRAVFAARKTEKGLLLGFSQANSHHIVAIEECPVTSPGIVSRFDAIRAIGLSMVANAEPF
HHHEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RITVLETLSGLDISVEGIKSVNDKQRRTLTETVLAMRGIARVSLSGEILIEPQKPIIEFG
EEEEEHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCEEC
GIPVSPPAGGFTQATKQAEDAMAELMLAHVGKSKRIADLFCGSGTFALRLARIGRVHAVE
CEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCHHHHHHHHHCCEEEEC
AEDKALKALDFAARNTQGLKPVSVEKRDLFRRPLMTSELKNYDAVVFDPPRAGAEVQCKE
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCHHHH
LARSTVKKIVAVSCNPLTLARDLAILTEGGYRVTRVTPVDQFLWSPHVEAVAVLEK
HHHHHHHHHHHCCCCCCHHHHHEEEEECCCEEEEEECCHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194