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The map label for this gene is hisZ

Identifier: 15888021

GI number: 15888021

Start: 672807

End: 673934

Strand: Direct

Name: hisZ

Synonym: Atu0678

Alternate gene names: 15888021

Gene position: 672807-673934 (Clockwise)

Preceding gene: 15888020

Following gene: 15888022

Centisome position: 23.68

GC content: 64.18

Gene sequence:

>1128_bases
ATGCCCCTGATCGACATGCCGGAATTTGCCGGAGAGCTACTGGAAGAATTCGCCGCGCGCCGCACAAGCCGCGTGAACAC
GCCTGTGATCCAGCCCGCCGAACCGTTTCTGGATATGGCCGGCGAGGATTTGCGCCGCCGTATCTTCATGACGGAGAGCG
AAACGGGTGAGAGCCTCTGCCTGCGCCCCGAATTCACCATCCCCGTCTGCCTGCGCCATATCGAGACCGCGACCGGCACG
CCGAAGCGTTATTCCTATCTCGGTGAAGTCTTCCGCCAGCGCCGTGAGGGTGCGAGCGAGTTTTATCAGGCCGGCATCGA
AGACCTCGGCGATACCGATATTGCCGCCGCCGATGCCCGTGTCGTCATCGACGCGACCGCCATTCTGCAGCGTCTGCTGC
CCGGCCGCTCGCTTGCCGTCACGCTGGGCGACCAGCAGGTGTTTGAAGCCGTTGTCGCAGCGCTTGGCCTGCCGCTCGGC
TGGCAGAAACGGCTGGTGCAGGCTTTCGGCGACATGGCGCAGCTCGACGCGCTGCTGGAAAGCCTCGTGCATCCCAAGCC
GATGACGGGGCTGGACGCCCGGGTCGCCGGCCTTCTGGCGACGGGCGACGAGGCGGTGCTGGTCGATTATCTCGATACGG
TGATGCAGGAAACCGGCTATTCCACCAATGCCAGCCGCTCACCGCTCGAAATCGCCCGCCGTCTGCGGGAGAAGCTCGCG
CTCGCGGCGACGCGCCTGCCGGATGAGAGCTTCGAACTGCTCAAACAGTTCCTGGCCTTGCAGGCGCCCCTGCCGCAGGC
CTCGCAGGTTCTTGGCGATTTCGCCGCAAGGGCGAAGCTGAAGCTGGACGGCGCGCTTTCCGCTTTCGATAAACGCGTTG
CGGCCCTTGCCAATGCCGGTGTCGATCTTGAAACCGTCACCTATGGTGCCGCCTTTGGCCGCCCGCTCGATTATTATACT
GGCCTCGTTTTCGAGGTGGTGGAGGCGGGCAGCGACAGCGTTCTGGCCGGCGGCGGCCGTTACGACCGGCTGCTTACGCT
TCTCGGCGCACAGGAAAAAATACCGGCCGTGGGCTTCTCGCTCTGGCTGGATCGCATCAAAGCGGTGCGTGGGAGCGACA
AACCATGA

Upstream 100 bases:

>100_bases
GAGCCGCTTGAAGGATATCACCGGTTTTATGTCCATGATCCCTTCGGAAACCGCATCGAAATGATGCAGCCGCTCGAACC
GTGACGAAAAGTAACGTTCC

Downstream 100 bases:

>100_bases
TCACCATCGCATTGCCCTCAAAAGGCCGGATGAAGGAAGACGCCTCCGCCGTTCTGGAACGCGCCGGGCTGAAGGTTGCG
TCAGTCGGTAACGACCGCTC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLCLRPEFTIPVCLRHIETATGT
PKRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADARVVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLG
WQKRLVQAFGDMAQLDALLESLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLA
LAATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAGVDLETVTYGAAFGRPLDYYT
GLVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFSLWLDRIKAVRGSDKP

Sequences:

>Translated_375_residues
MPLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLCLRPEFTIPVCLRHIETATGT
PKRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADARVVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLG
WQKRLVQAFGDMAQLDALLESLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLA
LAATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAGVDLETVTYGAAFGRPLDYYT
GLVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFSLWLDRIKAVRGSDKP
>Mature_374_residues
PLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLCLRPEFTIPVCLRHIETATGTP
KRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADARVVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLGW
QKRLVQAFGDMAQLDALLESLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLAL
AATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAGVDLETVTYGAAFGRPLDYYTG
LVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFSLWLDRIKAVRGSDKP

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_AGRT5 (Q8UHK2)

Other databases:

- EMBL:   AE007869
- PIR:   AH2659
- PIR:   F97441
- RefSeq:   NP_353702.1
- ProteinModelPortal:   Q8UHK2
- STRING:   Q8UHK2
- GeneID:   1132716
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0678
- eggNOG:   COG3705
- HOGENOM:   HBG334668
- OMA:   PAEPFLD
- ProtClustDB:   PRK12295
- BioCyc:   ATUM176299-1:ATU0678-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR004516
- PANTHER:   PTHR11476

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 40756; Mature: 40625

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLC
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCEE
LRPEFTIPVCLRHIETATGTPKRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADAR
ECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCH
VVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLGWQKRLVQAFGDMAQLDALLE
HEEEHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
SLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLA
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAG
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLETVTYGAAFGRPLDYYTGLVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFS
CCHHHEEHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCHHCCCHHHHH
LWLDRIKAVRGSDKP
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
PLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLC
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCEE
LRPEFTIPVCLRHIETATGTPKRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADAR
ECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCH
VVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLGWQKRLVQAFGDMAQLDALLE
HEEEHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
SLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLA
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAG
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLETVTYGAAFGRPLDYYTGLVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFS
CCHHHEEHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCHHCCCHHHHH
LWLDRIKAVRGSDKP
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194