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The map label for this gene is glyS

Identifier: 15887985

GI number: 15887985

Start: 631738

End: 633891

Strand: Direct

Name: glyS

Synonym: Atu0644

Alternate gene names: 15887985

Gene position: 631738-633891 (Clockwise)

Preceding gene: 159184402

Following gene: 159184403

Centisome position: 22.23

GC content: 61.56

Gene sequence:

>2154_bases
ATGCCCGATCTTCTGCTTGAACTCCGCTCCGAGGAAATCCCCGCCCGCATGCAGCGCAAGGCGGCGGGCGATCTGAAGAA
ACTCGTCACCGACGCTCTGGTGGAGAGAGGGCTGACCTATGAGGGGGCGCGCGAATATTGGACGCCGCGCCGCCTCACGC
TGGATATTCGTGGCCTCAACGCCCGCTCCGCCGATGTGCGCGAGGAAAAGAAGGGCCCGCGCACCGATGCCAATGAAAAG
GCCATCGAGGGCTTTCTGCGCGGTGCCGGCCTGAACGATATCTCGGAAGCGCAGGTCGTTTCCGATCCGAAGAAGGGCGA
TTTCTACATCGCCATCATCAACAAGCCCGGCCGCCCGGCGGAAGAGATCATCGCCGAGGTGATGCCCGGCATCATCCGCT
CCTTCCCCTGGCCGAAATCCATGCGCTCCGGCCCGGCTTCCATGCCGAAGGGTTCAAGCTATGCCGGCATCGAGGGCAAG
GGCTCGGAAAGCCTGCGCTGGGTGCGTCCGCTGCAATCCATCGTCTGCCTGTTCGGCCCCGAACATGACGAAACCCAGGT
CATTCCCTTCGTGATCGATGGCATCGTCGCGGGCAACATCACCTATGGTCACCGTTTCCACGCGCCCGGCCCGATCACCG
TGCGCCGTTTCGAGGATTATGTCTCGAACCTTGAAAAGGCGAAGGTCATTCTCGATGCCGATCGCCGCAAGGATATCATC
CTGCACGATGCCAAGGATCTAGCCTTCGCCAACGGTCTTGAGCTGGTGGAAGACGAAGGCCTGCTGGAAGAAGTCTCTGG
CCTCGTCGAATGGCCGCAGGTGCTGATGGGCACTTTCGAGGAGGATTATCTGCAAATCCCGGCGGAAATCATCCGGCTGA
CCATTAAGACCAACCAGAAGTGTTTCGTTACCCGCAATCAGGGTGCGGAAGAGGGTCTTTCCAACCGCTTCATCCTGATC
TCGAACATCGAGGCAAGCGACGGCGGCAAGGAAATCATCCATGGCAACGGCAAGGTCGTGCGCGCGCGTCTTTCGGATGC
CCGCCATTTCTGGAACCGCGATCAGGGCGATCTGCCCGATCTGGAAACACTCAAAGATTCGGCCGCAAAATTCGACCTCG
ATCTGAAAAAGCCGCTCGACCAGCGCATGGCAAAGCTGGATGCGCTGAACGTTACCTTCCATGCCAAGCTCGGCACGCAG
GGTGAACGTGTGGCCCGCATCCGCGAACTGGCAAAGGCACTAGCCCCTGTCGTCGGGGCCGATGGAGCGCTGGTAGACCG
TGCGGTGGTGCTGGCGAAAGCCGACCTGCGCACGGAGGCAGTCGGAGAATTCCCCGAGCTTCAGGGCCTGATGGGCCGCA
AATATGCGGTGCTGCAGGGCGAAAACGAAAGCGTCGCCGCCGCCATTGAAGACCACTATAAGCCGCAGGGTCCCTCCGAC
CGCCTGCCAGCCGACAAGGTGGCGATCACTGTTGCTCTCGCCGACAAGCTCGATACGCTCGTCGGTTTTTGGGCCATCGA
TGAGAAGCCGACCGGTTCGAAGGACCCCTTCGCTCTGCGGCGCGCGGCGCTGGGTGTGGTGCGTATTCTGCTCGAGAAGA
ACGTCCGCCTGCCGCTGCTGAGCGTTGCGCGGGATTCAGACCTTCTCTCCTTCTTCCACGACCGCCTGAAGGTCTACCTG
CGCGATCTCGGCGCCCGTTATGACCTGATCGACGCCGTCCTCACACCGGAATCCGATGATCTGCTGATGATCGCCCGCCG
GGTCGAGGCGCTCACCGCCTTTATTACCGGTGAAGATGGCAGGAACCTTCTGGCAGGCGCCAAGCGCGCCACGCAGCTTC
TGGCCGCCGAGGAAAAGAAGGGCACCGTGGTGGCCGATGGCGTCTCGGAAGAGCTCTTGAAGCTCGACGCCGAAAAGGCG
CTTTACGCCGCGATCAAGACCGCCTCTGCCGATGCCGCAAAGGCCGTCGAGGGTGAGGATTTCCGCTCCGCCATGCAGGC
GCTTTCGACGCTGCGCGCGCCCGTCGACAAGTTCTTCGAGGATGTGCTCGTGAATGACGAAGATGCGGCCATCCGCGCCA
ATCGTCTGGCGCTGTTGAAGGCCATTCGCGAGGCGACCGGCACGGTTGCGGATTTCTCGAAGATCACGGGGTGA

Upstream 100 bases:

>100_bases
TGCGCGCCGGCGGTAATTTAACCGAATGCATTGGTGACTTTTGCCGCGTTGCTTGCTAAGCCGACCGCAATCGTCCGACA
ACAGATAAAGTCCTGATCCC

Downstream 100 bases:

>100_bases
GGGGTTTGGGGGTAAACGGGCATTGCAGAATGCTGGGTTTCACCCCCCTCTGTCCTGCCGGACATCTCCCCCACAAGGGG
GGAGATCGGATCGTTGCTAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 717; Mature: 716

Protein sequence:

>717_residues
MPDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLNARSADVREEKKGPRTDANEK
AIEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPAEEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGK
GSESLRWVRPLQSIVCLFGPEHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDII
LHDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQKCFVTRNQGAEEGLSNRFILI
SNIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPDLETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQ
GERVARIRELAKALAPVVGADGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSD
RLPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLLSVARDSDLLSFFHDRLKVYL
RDLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDGRNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKA
LYAAIKTASADAAKAVEGEDFRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG

Sequences:

>Translated_717_residues
MPDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLNARSADVREEKKGPRTDANEK
AIEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPAEEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGK
GSESLRWVRPLQSIVCLFGPEHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDII
LHDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQKCFVTRNQGAEEGLSNRFILI
SNIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPDLETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQ
GERVARIRELAKALAPVVGADGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSD
RLPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLLSVARDSDLLSFFHDRLKVYL
RDLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDGRNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKA
LYAAIKTASADAAKAVEGEDFRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG
>Mature_716_residues
PDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLNARSADVREEKKGPRTDANEKA
IEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPAEEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGKG
SESLRWVRPLQSIVCLFGPEHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDIIL
HDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQKCFVTRNQGAEEGLSNRFILIS
NIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPDLETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQG
ERVARIRELAKALAPVVGADGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSDR
LPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLLSVARDSDLLSFFHDRLKVYLR
DLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDGRNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKAL
YAAIKTASADAAKAVEGEDFRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=734, Percent_Identity=34.0599455040872, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_AGRT5 (A9CK39)

Other databases:

- EMBL:   AE007869
- PIR:   AF2655
- PIR:   B97437
- RefSeq:   NP_353666.1
- ProteinModelPortal:   A9CK39
- STRING:   A9CK39
- GeneID:   1132682
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0644
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A9CK39
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78715; Mature: 78584

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLN
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCC
ARSADVREEKKGPRTDANEKAIEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPA
CCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCH
EEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGKGSESLRWVRPLQSIVCLFGP
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
EHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDII
CCCCCEEHHEEHHCEEECCEEECCEECCCCCEEHHHHHHHHHHHHHHEEEEECCCCCCEE
LHDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQK
EECCHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEEECCCE
CFVTRNQGAEEGLSNRFILISNIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPD
EEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHCCCCCCCCC
LETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRELAKALAPVVGA
HHHHHHHHHHCCCHHHCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
DGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSD
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCCC
RLPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLL
CCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
SVARDSDLLSFFHDRLKVYLRDLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDG
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCC
RNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKALYAAIKTASADAAKAVEGED
CHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
FRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
PDLLLELRSEEIPARMQRKAAGDLKKLVTDALVERGLTYEGAREYWTPRRLTLDIRGLN
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCC
ARSADVREEKKGPRTDANEKAIEGFLRGAGLNDISEAQVVSDPKKGDFYIAIINKPGRPA
CCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCH
EEIIAEVMPGIIRSFPWPKSMRSGPASMPKGSSYAGIEGKGSESLRWVRPLQSIVCLFGP
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
EHDETQVIPFVIDGIVAGNITYGHRFHAPGPITVRRFEDYVSNLEKAKVILDADRRKDII
CCCCCEEHHEEHHCEEECCEEECCEECCCCCEEHHHHHHHHHHHHHHEEEEECCCCCCEE
LHDAKDLAFANGLELVEDEGLLEEVSGLVEWPQVLMGTFEEDYLQIPAEIIRLTIKTNQK
EECCHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEEECCCE
CFVTRNQGAEEGLSNRFILISNIEASDGGKEIIHGNGKVVRARLSDARHFWNRDQGDLPD
EEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHCCCCCCCCC
LETLKDSAAKFDLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRELAKALAPVVGA
HHHHHHHHHHCCCHHHCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
DGALVDRAVVLAKADLRTEAVGEFPELQGLMGRKYAVLQGENESVAAAIEDHYKPQGPSD
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCCC
RLPADKVAITVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALGVVRILLEKNVRLPLL
CCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
SVARDSDLLSFFHDRLKVYLRDLGARYDLIDAVLTPESDDLLMIARRVEALTAFITGEDG
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCC
RNLLAGAKRATQLLAAEEKKGTVVADGVSEELLKLDAEKALYAAIKTASADAAKAVEGED
CHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
FRSAMQALSTLRAPVDKFFEDVLVNDEDAAIRANRLALLKAIREATGTVADFSKITG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743194; 11743193