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The map label for this gene is kdsB

Identifier: 15887454

GI number: 15887454

Start: 103891

End: 104646

Strand: Reverse

Name: kdsB

Synonym: Atu0100

Alternate gene names: 15887454

Gene position: 104646-103891 (Counterclockwise)

Preceding gene: 159184156

Following gene: 15887453

Centisome position: 3.68

GC content: 58.99

Gene sequence:

>756_bases
ATGAAGAATCGGGATTTCGAGAAAGCGGTGGTCCTTATACCGGCCCGGATGGCATCCACACGCCTTCCAGGCAAGCCGCT
CGCGGATATTGGCGGCAGGCCAATGATCGTGCAGGTGGCGTTGCGCGCACGCGAGGCCGGTGCGGAACGAATCGTTGTCG
CAGTCGACGACGAGCAGGTATTTGCAGCCGTTCAAAACGCCGGTTTCGACGTGATGATGACACGCGGCGACCACCAGTCC
GGCTCCGACCGCATTTTCGAGGCGCTGCAAAAGGCCGACCCCTATGGCAAGGCGGAATACGTCATCAACGTCCAAGGCGA
CCTTCCGACCATCGAGGCGGAAACTATCCGTGCCTCGCTACGCCCGATGGAAAATGCTGCCGTCGATATTGCGACGTTAA
CGGTTGAAATCACCGACGAAGAGGAAAAAACCAATCCGAACGTGGTGAAAGTCGTTGGCAGCCCGCTTTCCGAAACACGA
TTGCGCGCGCTTTATTTCACCCGCACCACCGCGCCTTATGGTGATGGGCCGCTTTATCACCACATCGGGCTTTACACCTA
TCGCCGCGCCGCACTTGAAACATTCGTGCGCCTGCCGCCCTCGCCTCTGGAATTGCGTGAGCGGCTGGAACAGCTGCGTG
CGTTGGAAGCGGGCATGCGCATCGATGCGGAAATCGTCCGGTCTGTTCCGCTTGGCGTCGACACGCCGCACGATCTCGAA
AAAGCCCGCAAGATTCTTGCAGGCAGAACCCTCTAA

Upstream 100 bases:

>100_bases
ACTCGAAAGCTATGGCTTTTGCACAGCCTGCGCAACTTGAATGATGGTCCCCACTCCGTGACGTTTTGACACTTTTCGGG
CGGGATTTGAAGGGTGTCGG

Downstream 100 bases:

>100_bases
CGGACAGTCCCATGAATTTGATTACAAATCGCATTGCCTTTCAGGGCGAGTTCGGCGCGAATTCCGACATGGCATGCCGT
GACATGTTTCCGGATATGGA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKNRDFEKAVVLIPARMASTRLPGKPLADIGGRPMIVQVALRAREAGAERIVVAVDDEQVFAAVQNAGFDVMMTRGDHQS
GSDRIFEALQKADPYGKAEYVINVQGDLPTIEAETIRASLRPMENAAVDIATLTVEITDEEEKTNPNVVKVVGSPLSETR
LRALYFTRTTAPYGDGPLYHHIGLYTYRRAALETFVRLPPSPLELRERLEQLRALEAGMRIDAEIVRSVPLGVDTPHDLE
KARKILAGRTL

Sequences:

>Translated_251_residues
MKNRDFEKAVVLIPARMASTRLPGKPLADIGGRPMIVQVALRAREAGAERIVVAVDDEQVFAAVQNAGFDVMMTRGDHQS
GSDRIFEALQKADPYGKAEYVINVQGDLPTIEAETIRASLRPMENAAVDIATLTVEITDEEEKTNPNVVKVVGSPLSETR
LRALYFTRTTAPYGDGPLYHHIGLYTYRRAALETFVRLPPSPLELRERLEQLRALEAGMRIDAEIVRSVPLGVDTPHDLE
KARKILAGRTL
>Mature_251_residues
MKNRDFEKAVVLIPARMASTRLPGKPLADIGGRPMIVQVALRAREAGAERIVVAVDDEQVFAAVQNAGFDVMMTRGDHQS
GSDRIFEALQKADPYGKAEYVINVQGDLPTIEAETIRASLRPMENAAVDIATLTVEITDEEEKTNPNVVKVVGSPLSETR
LRALYFTRTTAPYGDGPLYHHIGLYTYRRAALETFVRLPPSPLELRERLEQLRALEAGMRIDAEIVRSVPLGVDTPHDLE
KARKILAGRTL

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=247, Percent_Identity=48.582995951417, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_AGRT5 (A9CKP8)

Other databases:

- EMBL:   AE007869
- PIR:   AG2588
- PIR:   G97370
- RefSeq:   NP_353135.1
- ProteinModelPortal:   A9CKP8
- SMR:   A9CKP8
- STRING:   A9CKP8
- GeneID:   1132138
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0100
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   A9CKP8
- ProtClustDB:   PRK05450
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27755; Mature: 27755

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRDFEKAVVLIPARMASTRLPGKPLADIGGRPMIVQVALRAREAGAERIVVAVDDEQV
CCCCCCCCEEEEEECHHHHCCCCCCCHHHCCCCCEEEHEEHHHHHCCCCEEEEEECCHHH
FAAVQNAGFDVMMTRGDHQSGSDRIFEALQKADPYGKAEYVINVQGDLPTIEAETIRASL
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
RPMENAAVDIATLTVEITDEEEKTNPNVVKVVGSPLSETRLRALYFTRTTAPYGDGPLYH
CCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHEEEEEEEECCCCCCCCCHHH
HIGLYTYRRAALETFVRLPPSPLELRERLEQLRALEAGMRIDAEIVRSVPLGVDTPHDLE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHH
KARKILAGRTL
HHHHHHCCCCC
>Mature Secondary Structure
MKNRDFEKAVVLIPARMASTRLPGKPLADIGGRPMIVQVALRAREAGAERIVVAVDDEQV
CCCCCCCCEEEEEECHHHHCCCCCCCHHHCCCCCEEEHEEHHHHHCCCCEEEEEECCHHH
FAAVQNAGFDVMMTRGDHQSGSDRIFEALQKADPYGKAEYVINVQGDLPTIEAETIRASL
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
RPMENAAVDIATLTVEITDEEEKTNPNVVKVVGSPLSETRLRALYFTRTTAPYGDGPLYH
CCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHEEEEEEEECCCCCCCCCHHH
HIGLYTYRRAALETFVRLPPSPLELRERLEQLRALEAGMRIDAEIVRSVPLGVDTPHDLE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHH
KARKILAGRTL
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194