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The map label for this gene is pnpA [H]

Identifier: 15887437

GI number: 15887437

Start: 84465

End: 86606

Strand: Reverse

Name: pnpA [H]

Synonym: Atu0082

Alternate gene names: 15887437

Gene position: 86606-84465 (Counterclockwise)

Preceding gene: 15887438

Following gene: 159184152

Centisome position: 3.05

GC content: 59.71

Gene sequence:

>2142_bases
ATGTTCAATAAACACTCCGTGGAAATCGAATGGGCAGGCCGCCCGTTGAAGCTCGAGACCGGCAAGGTTGCCCGTCAGGC
TGACGGCGCCGTCATCGCAACCTACGGCGAGACGATGGTTCTCGCGACCGTGGTTTCCGCAAAGTCTCCGAAGCCCGGCC
AGGACTTCTTCCCGCTGACCGTCAACTATCAGGAAAAGACCTACGCAGCCGGCAAGATCCCCGGTGGCTATTTCAAGCGC
GAAGGTCGTCCGTCGGAAAAGGAAACACTGGTTTCCCGCCTGATCGACCGTCCGATCCGCCCGCTTTTCCCTGAAGGTTA
CAAGAACGACACGCAGGTTGTCGTCACGGTCATTCAGCACGATCTGGAAAATGATCCGGACGTTCTGTCGATGGTTGCCG
CATCCGCTGCGCTCACACTGTCCGGCATTCCTTTCATGGGTCCGGTCGGCGGCGCGCGTGTTGGCTACATCAACGGCGAA
TACGTTCTGAACCCGCATCTCGACGAGATGGATGAATCGGTTCTGGATCTGGTTGTTGCCGGCACGCAGGACGCCGTCCT
GATGGTGGAATCGGAAGCCAAGGAACTCAACGAAGAGATCATGCTCGGCGCCGTCATGTTCGGCCACAAGGGCTTCCAGC
CGGTTATCGACGCGATCATCAAGCTCGCTGAAGTTGCCGCCAAGGAGCCACGCGAATTCGAGCCGGAGGATTTCTCCGCA
CTCGAAAACGAAATGCTCGGCCTTGCCGAAACCGAACTGCGCACCGCCTACAAGATCACCGAAAAGGCTGCCCGTTACGC
TGCCGTCGACGCCGTCAAGACGAAGGTCAAGGCGCACTTCCTGCCGGAAGAAGGCGAAGCGAAGTACAGCCCGGAAGAAA
TCGGCGCTGTCTTCAAGCACCTTCAGGCCAAGATCGTTCGCTGGAACGTTCTCGACACCAAGAGCCGCATCGACGGCCGC
GACCTGTCGACCGTTCGCCCGATCGTTTCCGAAGTCGGCATTCTGCCGCGCACCCACGGTTCGGCGCTGTTCACTCGTGG
TGAAACGCAGGCGATCGTTGTTGCGACGCTCGGCACAGGCGAAGACGAACAGTATGTCGATAGCCTGACCGGCATGTACA
AGGAACGTTTCCTGCTGCATTACAACTTCCCGCCCTACTCGGTCGGCGAAACAGGCCGCATGGGTTCCCCGGGCCGTCGC
GAAATCGGTCACGGCAAGCTCGCATGGCGCGCTATCCGTCCGATGCTGCCGACTGCGGAACAGTTCCCCTACACGCTGCG
CGTCGTTTCCGAAATCACCGAGTCCAACGGCTCCTCGTCGATGGCAACGGTTTGCGGCACCTCGCTTGCTCTGATGGACG
CCGGCGTGCCGCTTGCTAAGCCGGTTGCCGGTATCGCCATGGGCCTGATCCTTGAGGGCGAGCGCTTTGCGGTTCTCTCC
GACATCCTGGGTGACGAAGATCACCTCGGCGACATGGACTTCAAGGTTGCGGGTACGGCCGACGGCATCACCTCGCTGCA
GATGGACATCAAGATTGCCGGTATCACCGAAGAGATCATGAAGATCGCTCTGGAGCAGGCACAGGGTGGCCGCAAGCACA
TCCTCGGCGAAATGGCCAACGCCATCACCGAGAGCCGTGGCCAGCTCGGCGAATTCGCACCGCGCATCGAAGTCATGAAC
ATTCCGGTCGACAAGATTCGTGAAGTCATCGGTTCGGGCGGCAAGGTCATTCGCGAAATCGTCGAAAAGACCGGCGCCAA
GATCAACATCGAAGACGACGGCACCGTCAAGATCGCTTCTGCTTCCGGCAAGGAAATCGAAGCGGCCCGCAAGTGGATCC
ACTCGATCGTTGCGGAACCGGAAGTCGGCCAGATCTACGAAGGCACTGTTGTCAAGACCGCTGACTTCGGCGCTTTCGTC
AACTTCTTCGGCGCCCGTGACGGCCTCGTCCACATCTCGCAGCTCGCTTCCGAGCGCGTTGCCAAGACCTCCGACGTCGT
CAAGGAAGGGGACAAGGTCTGGGTCAAGCTGATGGGCTTTGATGAGCGCGGCAAGGTTCGCCTGTCCATGAAGGTTGTCG
ACCAGGCAACCGGCAAGGAAGTGGCTGCCGACAAGAAAGACGGCGAAGCCGCTGCCGAATAA

Upstream 100 bases:

>100_bases
GGATTGCAGGATGCTTGATCTTGAAAGCCTCCCGCTGTCTTGCCCGTGATTGCGTCATTCGTTGCCCCTTTTTGGTGGCA
CGCACAAACAAGGACAAGAT

Downstream 100 bases:

>100_bases
GGCTGCGCCGCCAGACCCTATCGAGGCGCGGGTTCTGCCCGCGCCTTTTTTTCGTTTTTGTTATCGTTTGTCTTTTCGGG
AAAACCGGTTTCCACTTTTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQHDLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGE
YVLNPHLDEMDESVLDLVVAGTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKHLQAKIVRWNVLDTKSRIDGR
DLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMANAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE

Sequences:

>Translated_713_residues
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQHDLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGE
YVLNPHLDEMDESVLDLVVAGTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKHLQAKIVRWNVLDTKSRIDGR
DLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMANAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE
>Mature_713_residues
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQHDLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGE
YVLNPHLDEMDESVLDLVVAGTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKHLQAKIVRWNVLDTKSRIDGR
DLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMANAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=38.0952380952381, Blast_Score=461, Evalue=1e-129,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=48.8372093023256, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=45.6521739130435, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=57.514450867052, Blast_Score=793, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=726, Percent_Identity=33.3333333333333, Blast_Score=354, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17535281, Length=90, Percent_Identity=45.5555555555556, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=36.697247706422, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362905, Length=737, Percent_Identity=37.584803256445, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=737, Percent_Identity=37.584803256445, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=737, Percent_Identity=37.584803256445, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=680, Percent_Identity=37.2058823529412, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=45.0549450549451, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77358; Mature: 77358

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLT
CCCCEEEEEEECCCCEEECCCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGEYVLNPHLDEMDESVLDLVVA
HCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHHC
GTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
CCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKH
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
LQAKIVRWNVLDTKSRIDGRDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTG
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPTAE
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
QFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
HCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHEECCCCHHHHH
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMAN
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHH
AITESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIAS
HHHHHHCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEEC
ASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFVNFFGARDGLVHISQLASERV
CCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHH
AKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE
HHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLT
CCCCEEEEEEECCCCEEECCCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGEYVLNPHLDEMDESVLDLVVA
HCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHHC
GTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
CCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKH
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
LQAKIVRWNVLDTKSRIDGRDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTG
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPTAE
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
QFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
HCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHEECCCCHHHHH
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMAN
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHH
AITESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIAS
HHHHHHCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEEC
ASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFVNFFGARDGLVHISQLASERV
CCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHH
AKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE
HHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA