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The map label for this gene is buk2 [H]

Identifier: 239618522

GI number: 239618522

Start: 2293655

End: 2294746

Strand: Reverse

Name: buk2 [H]

Synonym: Kole_2163

Alternate gene names: 239618522

Gene position: 2294746-2293655 (Counterclockwise)

Preceding gene: 239618523

Following gene: 239618521

Centisome position: 99.68

GC content: 44.05

Gene sequence:

>1092_bases
ATGAAGAGAATTCTCGTGATAAATCCTGGATCCACATCGACAAAATTGGCTATCTTTGAAGATGCACGCGCCGTTGTTGA
CAAAACTATCCGGCATTCGGTTGAAGAATTATCTCCCTTCAACAGTTTGTACGAACAAAAGGATTTTCGGAAAAATTTGA
TAATAAACTTTCTGAAGGATTCCGGTTATTGCCTTGAAGATTTCGACGCTTTCGTGGGTAGAGGAGGTCTTTTAGCTCCT
ATTCCCGGTGGAACCTATCTGGTAACAGAAAGAATGATACAGGACTTGAAGGAAGCGAAGTATGGTGAACACGCTTCCAA
CCTTGGTGGAATATTAGCAAAAGAGTTATCTATGGAATCGAAGAAGGAGATCCCGGCATATATAGCCGATCCTGTTGTTG
TCGATGAAATGCAGGAAGTGGCGAGACTTTCCGGGCATCCGGACTTCACTAGAAAATCTATATTTCATGCACTCAATCAA
AAAGCCGTCGCAAGATATGTCGCAGCAAAACACGGTAAAAAATATGAAGAAATGAACCTTATCGTCGTTCATATGGGTGG
TGGAATCTCGATAGGTGCCCATCGGAAGGGAAGGGTAGTAGATGTGAACAACGCACTCAACGGCGATGGACCATTTACCC
CTGAACGCGCTGGAACGCTTCCATTGACCGGGCTCATAAAACTCTGTTACAGTGGAAAATACTCAAAAGCCGAGATAAAG
AAACTCATCAAAGGAAAGGGTGGCCTGGTAGCCTACACAGGCATAAATGATTGTAGAAAGATACAGGACGCCATTAGCGA
CGGAAACAAAGAATGGGAATTCTACTACCGCGGTATGGCATATCAAATTATAAAGTGGATCGGAAAAATGGCAGCTGTAC
TAAAGGGTGATGTGGACGCTATCGTTCTTACAGGTGGAATCGCTTACGATGATGAATTCATGGTGAAGTGGATCGAGGAA
AAGGTTTCCTTCATCGCTCCGATAGAGGTAGTGGCTGGCGGAAATGAGGAAGAGGCTCTCGCAATTGCTGCATTAAGGGT
TTTGAATGGAGAGGAAGAGGCGAAGATTTATGAAACTGTTGCCATGGTTTGA

Upstream 100 bases:

>100_bases
TCGGTGGTAAAATCCCGATAATTCTGACTTCGCGAGCAGATTCCGACAGAACAAAACTCTATTCCATCGCTCTCAACGTT
CTTCTTTCGGAGGGATGACA

Downstream 100 bases:

>100_bases
AAACAGGATTTTAATATTCATGGGAATGTTCGGGAGCGGTAAGACGGAGATCGCACTGAACGTAGCCTTTCATCTTAAAG
AAAGTTTTGAAAAAGTTGCC

Product: butyrate kinase

Products: NA

Alternate protein names: BK 2; Branched-chain carboxylic acid kinase 2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKRILVINPGSTSTKLAIFEDARAVVDKTIRHSVEELSPFNSLYEQKDFRKNLIINFLKDSGYCLEDFDAFVGRGGLLAP
IPGGTYLVTERMIQDLKEAKYGEHASNLGGILAKELSMESKKEIPAYIADPVVVDEMQEVARLSGHPDFTRKSIFHALNQ
KAVARYVAAKHGKKYEEMNLIVVHMGGGISIGAHRKGRVVDVNNALNGDGPFTPERAGTLPLTGLIKLCYSGKYSKAEIK
KLIKGKGGLVAYTGINDCRKIQDAISDGNKEWEFYYRGMAYQIIKWIGKMAAVLKGDVDAIVLTGGIAYDDEFMVKWIEE
KVSFIAPIEVVAGGNEEEALAIAALRVLNGEEEAKIYETVAMV

Sequences:

>Translated_363_residues
MKRILVINPGSTSTKLAIFEDARAVVDKTIRHSVEELSPFNSLYEQKDFRKNLIINFLKDSGYCLEDFDAFVGRGGLLAP
IPGGTYLVTERMIQDLKEAKYGEHASNLGGILAKELSMESKKEIPAYIADPVVVDEMQEVARLSGHPDFTRKSIFHALNQ
KAVARYVAAKHGKKYEEMNLIVVHMGGGISIGAHRKGRVVDVNNALNGDGPFTPERAGTLPLTGLIKLCYSGKYSKAEIK
KLIKGKGGLVAYTGINDCRKIQDAISDGNKEWEFYYRGMAYQIIKWIGKMAAVLKGDVDAIVLTGGIAYDDEFMVKWIEE
KVSFIAPIEVVAGGNEEEALAIAALRVLNGEEEAKIYETVAMV
>Mature_363_residues
MKRILVINPGSTSTKLAIFEDARAVVDKTIRHSVEELSPFNSLYEQKDFRKNLIINFLKDSGYCLEDFDAFVGRGGLLAP
IPGGTYLVTERMIQDLKEAKYGEHASNLGGILAKELSMESKKEIPAYIADPVVVDEMQEVARLSGHPDFTRKSIFHALNQ
KAVARYVAAKHGKKYEEMNLIVVHMGGGISIGAHRKGRVVDVNNALNGDGPFTPERAGTLPLTGLIKLCYSGKYSKAEIK
KLIKGKGGLVAYTGINDCRKIQDAISDGNKEWEFYYRGMAYQIIKWIGKMAAVLKGDVDAIVLTGGIAYDDEFMVKWIEE
KVSFIAPIEVVAGGNEEEALAIAALRVLNGEEEAKIYETVAMV

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=237, Percent_Identity=25.7383966244726, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39968; Mature: 39968

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVINPGSTSTKLAIFEDARAVVDKTIRHSVEELSPFNSLYEQKDFRKNLIINFLKD
CCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGYCLEDFDAFVGRGGLLAPIPGGTYLVTERMIQDLKEAKYGEHASNLGGILAKELSMES
CCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KKEIPAYIADPVVVDEMQEVARLSGHPDFTRKSIFHALNQKAVARYVAAKHGKKYEEMNL
HHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IVVHMGGGISIGAHRKGRVVDVNNALNGDGPFTPERAGTLPLTGLIKLCYSGKYSKAEIK
EEEEECCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KLIKGKGGLVAYTGINDCRKIQDAISDGNKEWEFYYRGMAYQIIKWIGKMAAVLKGDVDA
HHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IVLTGGIAYDDEFMVKWIEEKVSFIAPIEVVAGGNEEEALAIAALRVLNGEEEAKIYETV
EEEECCCEECHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
AMV
HCC
>Mature Secondary Structure
MKRILVINPGSTSTKLAIFEDARAVVDKTIRHSVEELSPFNSLYEQKDFRKNLIINFLKD
CCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGYCLEDFDAFVGRGGLLAPIPGGTYLVTERMIQDLKEAKYGEHASNLGGILAKELSMES
CCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KKEIPAYIADPVVVDEMQEVARLSGHPDFTRKSIFHALNQKAVARYVAAKHGKKYEEMNL
HHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IVVHMGGGISIGAHRKGRVVDVNNALNGDGPFTPERAGTLPLTGLIKLCYSGKYSKAEIK
EEEEECCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KLIKGKGGLVAYTGINDCRKIQDAISDGNKEWEFYYRGMAYQIIKWIGKMAAVLKGDVDA
HHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IVLTGGIAYDDEFMVKWIEEKVSFIAPIEVVAGGNEEEALAIAALRVLNGEEEAKIYETV
EEEECCCEECHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
AMV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]