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The map label for this gene is mnmE [H]

Identifier: 239618515

GI number: 239618515

Start: 2288321

End: 2289643

Strand: Direct

Name: mnmE [H]

Synonym: Kole_2156

Alternate gene names: 239618515

Gene position: 2288321-2289643 (Clockwise)

Preceding gene: 239618514

Following gene: 239618516

Centisome position: 99.4

GC content: 43.92

Gene sequence:

>1323_bases
ATGATTCATGACCCTATTTGTGCCATTGCCACATCTCCCGGCATTTCTGCGTTGGGAATAATAAGGTGTTCCGGTGAAGG
AAGCTGGGAAAAGCTACTTCCCTTTGTCAATGTCACCGATCCCAAACCGTGGCGGCTGTATCTCACCCCCTTTACGATCA
ATGGTGAGATGATAGATGAAGTGATGATTGCGTTTTATAAGTCGCCAAAGTCCTACACTGGCGAGGATATGGTCGAGCTT
ATTTCACACGGGAGCCCCCTTATTCTGAGAATCATTCTGCAGGAGCTCATCAAAGTGGGGTTCAGGCAGGCGCTTCCGGG
AGAGTTCACAAAACGGGCCGTACTCAATGGCAAAATGGATTTGATAAAAGCAGAGTCAATAGAGACCCTTGTAAGTGCCC
ACAGCAAAAAGGCATTGAAAGCTGCTTTATCAAATTATTCTGGCAGACTTAGCCAGACTATAAATAGGATAAGAGAAACT
CTTTTGGAACTACTCGCAAAAGTTGAGGTAGAGCTCAATTATCCTGACGAAATTGAGGGCTCCGATGATGAAATAATTCG
GGAATTGGAAAAGACCCTGAGCAGGGTGAAGAAGCTGATCAGACACGGAGAAAACGGTATTGTGGCTGTCAACGGTGTAA
AAACAGTCATCGCGGGTAGAACGAATGTAGGGAAATCGACTTTGCTGAACGCTTTACTGAGACGAGACAGGGCAATTGTT
ACCGATATTCCCGGAACAACCCGCGACACCATCGAAGAAGACATAAGTATCAACGGTATATACTTTCGATTGGTTGATAC
GGCGGGGATTCGTGAGTCATCGGATAAAGTCGAAAGAATCGGTATTGAAAGAAGCTTAAAACAGATAAAGGAGGCCGACC
TTGTTCTTTTTATGGTCGATCTGATCGAACCCGAAAATGATCTAGAACTGTACGAGAAGCTGAGGAAAGAATTCAGGCAC
AGTATATTGATAGGCAACAAACTCGATATGGTCGAAAAATGCCCGGATGGATTCGATGTGTGTATAAGTGCAAAAACAGG
AAGAGGGATCGATAAACTTGAAAATCTCATGGTAGAAAGAACTCAGGATATCACTGAACTCTATCCAGATGAGGTCTTTG
TCACCGAAAGGCAACTTGCGCTTGTGCAGAGATCTGTGGAAATGATTGAAGAAATTCTTGAAGCCTTAAAGTCAGGTATT
ACTCCCGACGTTGTTGGATCATATCTTCAACAAATCATCTTTATCTACGATGAATTGACGGGACGATACTCCGCTGAAGA
TTTGCTGGACAAAATCTTCGGAAACTTTTGTGTAGGAAAGTAG

Upstream 100 bases:

>100_bases
AACATTTTCAACGAGAAGGAGTCAAAGGTTATCCTGTACAATATCAAGGAAGAAAAACTCCTTGATACAATAAAAGCTAT
CTTTCTTGGAGATGATAAAG

Downstream 100 bases:

>100_bases
GTGCGAAAATGATTTTAGGAATAGCTCTTCTGATAGCTGCCATGGTTTCCAGGTTTTTCCTTTCCCCGTTGTGGTACTTT
CTCGTGGCTGAAGCCCTTAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIHDPICAIATSPGISALGIIRCSGEGSWEKLLPFVNVTDPKPWRLYLTPFTINGEMIDEVMIAFYKSPKSYTGEDMVEL
ISHGSPLILRIILQELIKVGFRQALPGEFTKRAVLNGKMDLIKAESIETLVSAHSKKALKAALSNYSGRLSQTINRIRET
LLELLAKVEVELNYPDEIEGSDDEIIRELEKTLSRVKKLIRHGENGIVAVNGVKTVIAGRTNVGKSTLLNALLRRDRAIV
TDIPGTTRDTIEEDISINGIYFRLVDTAGIRESSDKVERIGIERSLKQIKEADLVLFMVDLIEPENDLELYEKLRKEFRH
SILIGNKLDMVEKCPDGFDVCISAKTGRGIDKLENLMVERTQDITELYPDEVFVTERQLALVQRSVEMIEEILEALKSGI
TPDVVGSYLQQIIFIYDELTGRYSAEDLLDKIFGNFCVGK

Sequences:

>Translated_440_residues
MIHDPICAIATSPGISALGIIRCSGEGSWEKLLPFVNVTDPKPWRLYLTPFTINGEMIDEVMIAFYKSPKSYTGEDMVEL
ISHGSPLILRIILQELIKVGFRQALPGEFTKRAVLNGKMDLIKAESIETLVSAHSKKALKAALSNYSGRLSQTINRIRET
LLELLAKVEVELNYPDEIEGSDDEIIRELEKTLSRVKKLIRHGENGIVAVNGVKTVIAGRTNVGKSTLLNALLRRDRAIV
TDIPGTTRDTIEEDISINGIYFRLVDTAGIRESSDKVERIGIERSLKQIKEADLVLFMVDLIEPENDLELYEKLRKEFRH
SILIGNKLDMVEKCPDGFDVCISAKTGRGIDKLENLMVERTQDITELYPDEVFVTERQLALVQRSVEMIEEILEALKSGI
TPDVVGSYLQQIIFIYDELTGRYSAEDLLDKIFGNFCVGK
>Mature_440_residues
MIHDPICAIATSPGISALGIIRCSGEGSWEKLLPFVNVTDPKPWRLYLTPFTINGEMIDEVMIAFYKSPKSYTGEDMVEL
ISHGSPLILRIILQELIKVGFRQALPGEFTKRAVLNGKMDLIKAESIETLVSAHSKKALKAALSNYSGRLSQTINRIRET
LLELLAKVEVELNYPDEIEGSDDEIIRELEKTLSRVKKLIRHGENGIVAVNGVKTVIAGRTNVGKSTLLNALLRRDRAIV
TDIPGTTRDTIEEDISINGIYFRLVDTAGIRESSDKVERIGIERSLKQIKEADLVLFMVDLIEPENDLELYEKLRKEFRH
SILIGNKLDMVEKCPDGFDVCISAKTGRGIDKLENLMVERTQDITELYPDEVFVTERQLALVQRSVEMIEEILEALKSGI
TPDVVGSYLQQIIFIYDELTGRYSAEDLLDKIFGNFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=30.3879310344828, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI193082993, Length=453, Percent_Identity=29.8013245033113, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI193082988, Length=511, Percent_Identity=27.7886497064579, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=37.0044052863436, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082120, Length=165, Percent_Identity=37.5757575757576, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=31.7073170731707, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=429, Percent_Identity=31.002331002331, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=31.7124735729387, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR008144
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49400; Mature: 49400

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHDPICAIATSPGISALGIIRCSGEGSWEKLLPFVNVTDPKPWRLYLTPFTINGEMIDE
CCCCCCEEEECCCCCCEEEEEEECCCCCHHHHCCCEECCCCCCEEEEEEEEEECHHHHHH
VMIAFYKSPKSYTGEDMVELISHGSPLILRIILQELIKVGFRQALPGEFTKRAVLNGKMD
HHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHH
LIKAESIETLVSAHSKKALKAALSNYSGRLSQTINRIRETLLELLAKVEVELNYPDEIEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
SDDEIIRELEKTLSRVKKLIRHGENGIVAVNGVKTVIAGRTNVGKSTLLNALLRRDRAIV
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHEEHCCCCCCCHHHHHHHHHHCCCCEE
TDIPGTTRDTIEEDISINGIYFRLVDTAGIRESSDKVERIGIERSLKQIKEADLVLFMVD
ECCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEPENDLELYEKLRKEFRHSILIGNKLDMVEKCPDGFDVCISAKTGRGIDKLENLMVER
HHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
TQDITELYPDEVFVTERQLALVQRSVEMIEEILEALKSGITPDVVGSYLQQIIFIYDELT
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GRYSAEDLLDKIFGNFCVGK
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIHDPICAIATSPGISALGIIRCSGEGSWEKLLPFVNVTDPKPWRLYLTPFTINGEMIDE
CCCCCCEEEECCCCCCEEEEEEECCCCCHHHHCCCEECCCCCCEEEEEEEEEECHHHHHH
VMIAFYKSPKSYTGEDMVELISHGSPLILRIILQELIKVGFRQALPGEFTKRAVLNGKMD
HHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHH
LIKAESIETLVSAHSKKALKAALSNYSGRLSQTINRIRETLLELLAKVEVELNYPDEIEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
SDDEIIRELEKTLSRVKKLIRHGENGIVAVNGVKTVIAGRTNVGKSTLLNALLRRDRAIV
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHEEHCCCCCCCHHHHHHHHHHCCCCEE
TDIPGTTRDTIEEDISINGIYFRLVDTAGIRESSDKVERIGIERSLKQIKEADLVLFMVD
ECCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEPENDLELYEKLRKEFRHSILIGNKLDMVEKCPDGFDVCISAKTGRGIDKLENLMVER
HHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
TQDITELYPDEVFVTERQLALVQRSVEMIEEILEALKSGITPDVVGSYLQQIIFIYDELT
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GRYSAEDLLDKIFGNFCVGK
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 11092873 [H]