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The map label for this gene is thiB [H]

Identifier: 239618513

GI number: 239618513

Start: 2284069

End: 2285055

Strand: Reverse

Name: thiB [H]

Synonym: Kole_2154

Alternate gene names: 239618513

Gene position: 2285055-2284069 (Counterclockwise)

Preceding gene: 239618517

Following gene: 239618512

Centisome position: 99.26

GC content: 43.67

Gene sequence:

>987_bases
ATGAAAAGGACTGTGTTGCTGATTCTTCTGATGTTCCTTGTGTCTTCACTGGTTTTATCTGAAAAACTGGTGGTTTACAC
CTACGACAGTCTGCTTCCTCTCGCGCGAGAAGCCTTTCCAAAATTTGAAGAGCTCACCGGGATAGAGGTTGTGGTGAGAA
CCTACGGTGACGCCGGAGCGGTTTTGTCAAAGGTCATTGCGGAGAGGGAAAAGTCAGATGTTGATGTGGTCATAGGTATC
GACAACCTACTGGCAAAGAGAGCCTTTGAAGAGGATATCTTCCTTAGCTACAAACCAAAGAATGCAGATTCGATTGTCGA
TAAGTCCCTGATTTTTGACCCCCAGTGGCGACTGACACCTTATGACTACGGAGCGATTGCAATAATCTATGACCACGCAG
AATATGGCAAACTCGATATCGAATCTCTTGACCAGCTAACCGAAAAACGATTCAGAAGAAGCCTCATCATTCAGGATCCA
AGAACTTCCAGTACAGGGTTGTCCTTTGTAATGTGGACATACCTATTACACCAGGAAGATGAGGATTTTGTCGAATTCTG
GCGAAATTTAAAGGATTCTATACTTACAATTACCTTTGGTTGGGATGATGCTTTTGAAAAGTTTGAAGCCGGAGAAGCAC
CCATGATGGTTAGCTATGCAACCGACGGAGCGTACTCCTACCACTATTATGGAAAGGTATATTATGGAGCCATCATTCCA
GGTGGAAAAGGTTACGTTCAAATCGAGGGAGCCGGAATCTGCAAATGGACGGATAATTTAGAGGCTGCTCAAAAATTCAT
CGATTTTCTTATTTCCCCGGAATGCCAGAACAGCATTCCGCTGAATCAGTGGATGTTCCCGGTTATAGAAATTGATCTGC
CAGAAGCGTTTAACTACGCGGTGAAACCTACCGAACTTCTCTCTGTTCCTGAAAATATCGATATTGAAAAAATTATTGCC
CTCTGGGAGAGTGTTATCTACGAATAA

Upstream 100 bases:

>100_bases
CGGCGCAGACCCCTTGAACCTGATCCGGGTAATACCGGCGAAGGGAGGGCGTTTTCGAACCGTCCCCCGGACGGTCATTT
TTTTTAGGGAGGGATTTAAA

Downstream 100 bases:

>100_bases
ATTCTATCTGGCTCTTTTTTTGACGTTAGCAATCGCGGTTCTCTTTATACTTTATCCATTGTTCGCTTCCTTTGGCTCCG
CCAGAGGAAGCGAAGTTTTG

Product: ABC transporter, periplasmic binding protein, thiB subfamily

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKRTVLLILLMFLVSSLVLSEKLVVYTYDSLLPLAREAFPKFEELTGIEVVVRTYGDAGAVLSKVIAEREKSDVDVVIGI
DNLLAKRAFEEDIFLSYKPKNADSIVDKSLIFDPQWRLTPYDYGAIAIIYDHAEYGKLDIESLDQLTEKRFRRSLIIQDP
RTSSTGLSFVMWTYLLHQEDEDFVEFWRNLKDSILTITFGWDDAFEKFEAGEAPMMVSYATDGAYSYHYYGKVYYGAIIP
GGKGYVQIEGAGICKWTDNLEAAQKFIDFLISPECQNSIPLNQWMFPVIEIDLPEAFNYAVKPTELLSVPENIDIEKIIA
LWESVIYE

Sequences:

>Translated_328_residues
MKRTVLLILLMFLVSSLVLSEKLVVYTYDSLLPLAREAFPKFEELTGIEVVVRTYGDAGAVLSKVIAEREKSDVDVVIGI
DNLLAKRAFEEDIFLSYKPKNADSIVDKSLIFDPQWRLTPYDYGAIAIIYDHAEYGKLDIESLDQLTEKRFRRSLIIQDP
RTSSTGLSFVMWTYLLHQEDEDFVEFWRNLKDSILTITFGWDDAFEKFEAGEAPMMVSYATDGAYSYHYYGKVYYGAIIP
GGKGYVQIEGAGICKWTDNLEAAQKFIDFLISPECQNSIPLNQWMFPVIEIDLPEAFNYAVKPTELLSVPENIDIEKIIA
LWESVIYE
>Mature_328_residues
MKRTVLLILLMFLVSSLVLSEKLVVYTYDSLLPLAREAFPKFEELTGIEVVVRTYGDAGAVLSKVIAEREKSDVDVVIGI
DNLLAKRAFEEDIFLSYKPKNADSIVDKSLIFDPQWRLTPYDYGAIAIIYDHAEYGKLDIESLDQLTEKRFRRSLIIQDP
RTSSTGLSFVMWTYLLHQEDEDFVEFWRNLKDSILTITFGWDDAFEKFEAGEAPMMVSYATDGAYSYHYYGKVYYGAIIP
GGKGYVQIEGAGICKWTDNLEAAQKFIDFLISPECQNSIPLNQWMFPVIEIDLPEAFNYAVKPTELLSVPENIDIEKIIA
LWESVIYE

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=317, Percent_Identity=31.8611987381703, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37528; Mature: 37528

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTVLLILLMFLVSSLVLSEKLVVYTYDSLLPLAREAFPKFEELTGIEVVVRTYGDAGA
CCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHH
VLSKVIAEREKSDVDVVIGIDNLLAKRAFEEDIFLSYKPKNADSIVDKSLIFDPQWRLTP
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEECCCCCCCC
YDYGAIAIIYDHAEYGKLDIESLDQLTEKRFRRSLIIQDPRTSSTGLSFVMWTYLLHQED
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCC
EDFVEFWRNLKDSILTITFGWDDAFEKFEAGEAPMMVSYATDGAYSYHYYGKVYYGAIIP
HHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEEEEC
GGKGYVQIEGAGICKWTDNLEAAQKFIDFLISPECQNSIPLNQWMFPVIEIDLPEAFNYA
CCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEECCHHCCCC
VKPTELLSVPENIDIEKIIALWESVIYE
CCCHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRTVLLILLMFLVSSLVLSEKLVVYTYDSLLPLAREAFPKFEELTGIEVVVRTYGDAGA
CCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHH
VLSKVIAEREKSDVDVVIGIDNLLAKRAFEEDIFLSYKPKNADSIVDKSLIFDPQWRLTP
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEECCCCCCCC
YDYGAIAIIYDHAEYGKLDIESLDQLTEKRFRRSLIIQDPRTSSTGLSFVMWTYLLHQED
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCC
EDFVEFWRNLKDSILTITFGWDDAFEKFEAGEAPMMVSYATDGAYSYHYYGKVYYGAIIP
HHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEEEEC
GGKGYVQIEGAGICKWTDNLEAAQKFIDFLISPECQNSIPLNQWMFPVIEIDLPEAFNYA
CCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEECCHHCCCC
VKPTELLSVPENIDIEKIIALWESVIYE
CCCHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]