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The map label for this gene is glyS [H]

Identifier: 239618467

GI number: 239618467

Start: 2239209

End: 2241260

Strand: Reverse

Name: glyS [H]

Synonym: Kole_2108

Alternate gene names: 239618467

Gene position: 2241260-2239209 (Counterclockwise)

Preceding gene: 239618468

Following gene: 239618466

Centisome position: 97.36

GC content: 40.98

Gene sequence:

>2052_bases
ATGAGTTCACATAAGGCACTGCTTGAAATCGGGATAGAGGAAATACCTGCCAGTGAAGTGAAAAGTATCGTTTCTCAGAT
AAACGGACGTTTGCCTAATTTATTAGAACAACAACGTATTAACTATTCAAACATCAAGACTTTCATAGCAAGCAGACGCT
TCGGCGTTTTGATCAAAGGATTGCCTGAGAAGCAGGAAGATTATTATCAGGAGAAACGCGGACCAGCTGAAAAGGTTGCT
TTCAAAGATGGTAAGCCTACGAAAGCCCTTGAAGGTTTTTTGAGATCTAACAATGCTACTGTCGACGATGTCGAAGTAAG
GGAATTCAAAGGCACATCCTACATTTTCTTGAAGAAAAAGGTTGAGGGTAAGAAAACCTCTCTGGTTCTTTCTGAGGTGT
TTAAGGAACTTATCCATGGACTACAATTCAAAAAACCGATGAGATGGGGAAATGGCGAACATAAATTCGTGAGGCCTGTA
AGATGGCTTGTAGCGTTGTTCGACGACGAGGTTCTGAACCTTGAGCTTTTCGATAAAAAAGCCTCAGACACGTCTTATGG
GCACCGTTTCTTCTTTGACAAAATAAAGGTTACCTCTGAAAATTATTTTGAGACCCTCAAAGAAGCGTTGGTTATAGCGG
ATTTCGATGAAAGAGCACAAATGGTTATAAATGAGCTTGAGAGAATTGAGAAAGAGAAAAACGTCACAATTCCCAGGGAC
GCTGAACTAATAGATGAAGTTACCTGCATTACAGAATTACCTACAGCTGTTTTAGGAAAATTTTTGCCCAAATATCTGTA
TCTACCAGACGAGGTCATCATAGTTACCATAAGGCACCATCAAAGAACTTTTCCTGTTTATGAAGGAAATAAATTAACGA
ATAGTTTCGTTGCCTTCCAGGACGGTCCTGCTGATCCCAAAGGAAATGTGAGAAGAGGATACGAAGAGGTTATAAATGCC
CGGCTAGAAGACGCGCATTTCTACTTTGAAAGAGATATTCAGAAACCATTGGAAGCATATGTTGAATACCTAAAAGGGAT
TCTGTTCCAGAGAGGTCTTGGAACTCTCTACGAAAAAACACAACGTGTTCGCTCGCTTTCGAAATATATCGCAAAGCAGT
TGAATGCAAAACCGGTAGAAGAAGAGGCTATAGACAGAACAGCTTTACTGGCAAAAGCGGACCTAACAACAAGGGTTGTT
CAGGAATTTCCGGAACTTCAGGGAATCATGGGGAGGATCTATGCCCTTGAATCCGGTGAGAGCAGCGAGGTTGCATGGGG
CATTCAGGAACATTACGAACCTCTAAATGAAGTTCCTGAAACCCTTACAGGTGCCGTTGTTGGTGTAGCCGATAGAATAG
ATACGCTGGTTGGAAATTTCCTGCTAGGAAATATTCCATCCGGTTCTAAAGATCCTTATGGATTGAGACGGAAATTATCG
CAAATCTTCGAGACGATGTACGCTTTTGGATGGAATATGAATCTTATCAAGCTCTTTGAGAAGGCAGCTGATAATCTGGG
AGAAGGTTACGAGAAAATCTCCACAACTCTTGAACCTTTTATAAAGTCCCGTTATGAAGCCTTCTTACTCGAAAAAGGCT
ATTCGATAAACATAGCCCGTGCTGTTTCAATGTGGTGGAAGAACCCATATCTTGGTGAACTTGTAGCAAAAGCGATTTCT
GAGATTATCGGACGTGATGATTTCGACAAATTGCTTGTAGCATACCAGAGAGTTCACAACATCAGCAAGAAGCATGATTC
CAATAAGTTTGACGGATCACTTTTCCTGAAAGAAGCAGAAGTAAATCTTTTCAATGCTTATGTCGAGGCTCTGGACAAAA
TAAAGAAAGCACTGGAAAAACGCGATTTCTCGGTAGCATTGAAAACCCTTATTGCCTTGAAAGAACCCATAGACGAATAT
TTTGATGAAGTCTTCGTTATGGACAGCCAGGTGGATATACGTCTGAATCGTCTTGGATTCTTGAAAGCTCTTGATGAGCT
TTTCTTTGAGATTGCTGACCTCTCATATCTGATAAATGAGGAACACAAATGA

Upstream 100 bases:

>100_bases
ACGCGATCAGCGTTGCGCAAAGACAAAGTTACATCAAATCCATTCGTGATATGGCAAGAGCTGTTGCCAGAGCTTATATT
CAGCAGGAGCGTGATAGCGA

Downstream 100 bases:

>100_bases
GCGAAAAAAGCGATTATAGATCAGGTATAGTTTCATTGGTTGGTAAGCCAAATGTGGGAAAATCCTCGCTGGTCAATCAA
CTGGTCGGCGAAAAGGTAGC

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MSSHKALLEIGIEEIPASEVKSIVSQINGRLPNLLEQQRINYSNIKTFIASRRFGVLIKGLPEKQEDYYQEKRGPAEKVA
FKDGKPTKALEGFLRSNNATVDDVEVREFKGTSYIFLKKKVEGKKTSLVLSEVFKELIHGLQFKKPMRWGNGEHKFVRPV
RWLVALFDDEVLNLELFDKKASDTSYGHRFFFDKIKVTSENYFETLKEALVIADFDERAQMVINELERIEKEKNVTIPRD
AELIDEVTCITELPTAVLGKFLPKYLYLPDEVIIVTIRHHQRTFPVYEGNKLTNSFVAFQDGPADPKGNVRRGYEEVINA
RLEDAHFYFERDIQKPLEAYVEYLKGILFQRGLGTLYEKTQRVRSLSKYIAKQLNAKPVEEEAIDRTALLAKADLTTRVV
QEFPELQGIMGRIYALESGESSEVAWGIQEHYEPLNEVPETLTGAVVGVADRIDTLVGNFLLGNIPSGSKDPYGLRRKLS
QIFETMYAFGWNMNLIKLFEKAADNLGEGYEKISTTLEPFIKSRYEAFLLEKGYSINIARAVSMWWKNPYLGELVAKAIS
EIIGRDDFDKLLVAYQRVHNISKKHDSNKFDGSLFLKEAEVNLFNAYVEALDKIKKALEKRDFSVALKTLIALKEPIDEY
FDEVFVMDSQVDIRLNRLGFLKALDELFFEIADLSYLINEEHK

Sequences:

>Translated_683_residues
MSSHKALLEIGIEEIPASEVKSIVSQINGRLPNLLEQQRINYSNIKTFIASRRFGVLIKGLPEKQEDYYQEKRGPAEKVA
FKDGKPTKALEGFLRSNNATVDDVEVREFKGTSYIFLKKKVEGKKTSLVLSEVFKELIHGLQFKKPMRWGNGEHKFVRPV
RWLVALFDDEVLNLELFDKKASDTSYGHRFFFDKIKVTSENYFETLKEALVIADFDERAQMVINELERIEKEKNVTIPRD
AELIDEVTCITELPTAVLGKFLPKYLYLPDEVIIVTIRHHQRTFPVYEGNKLTNSFVAFQDGPADPKGNVRRGYEEVINA
RLEDAHFYFERDIQKPLEAYVEYLKGILFQRGLGTLYEKTQRVRSLSKYIAKQLNAKPVEEEAIDRTALLAKADLTTRVV
QEFPELQGIMGRIYALESGESSEVAWGIQEHYEPLNEVPETLTGAVVGVADRIDTLVGNFLLGNIPSGSKDPYGLRRKLS
QIFETMYAFGWNMNLIKLFEKAADNLGEGYEKISTTLEPFIKSRYEAFLLEKGYSINIARAVSMWWKNPYLGELVAKAIS
EIIGRDDFDKLLVAYQRVHNISKKHDSNKFDGSLFLKEAEVNLFNAYVEALDKIKKALEKRDFSVALKTLIALKEPIDEY
FDEVFVMDSQVDIRLNRLGFLKALDELFFEIADLSYLINEEHK
>Mature_682_residues
SSHKALLEIGIEEIPASEVKSIVSQINGRLPNLLEQQRINYSNIKTFIASRRFGVLIKGLPEKQEDYYQEKRGPAEKVAF
KDGKPTKALEGFLRSNNATVDDVEVREFKGTSYIFLKKKVEGKKTSLVLSEVFKELIHGLQFKKPMRWGNGEHKFVRPVR
WLVALFDDEVLNLELFDKKASDTSYGHRFFFDKIKVTSENYFETLKEALVIADFDERAQMVINELERIEKEKNVTIPRDA
ELIDEVTCITELPTAVLGKFLPKYLYLPDEVIIVTIRHHQRTFPVYEGNKLTNSFVAFQDGPADPKGNVRRGYEEVINAR
LEDAHFYFERDIQKPLEAYVEYLKGILFQRGLGTLYEKTQRVRSLSKYIAKQLNAKPVEEEAIDRTALLAKADLTTRVVQ
EFPELQGIMGRIYALESGESSEVAWGIQEHYEPLNEVPETLTGAVVGVADRIDTLVGNFLLGNIPSGSKDPYGLRRKLSQ
IFETMYAFGWNMNLIKLFEKAADNLGEGYEKISTTLEPFIKSRYEAFLLEKGYSINIARAVSMWWKNPYLGELVAKAISE
IIGRDDFDKLLVAYQRVHNISKKHDSNKFDGSLFLKEAEVNLFNAYVEALDKIKKALEKRDFSVALKTLIALKEPIDEYF
DEVFVMDSQVDIRLNRLGFLKALDELFFEIADLSYLINEEHK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=31.6931982633864, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78425; Mature: 78294

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHKALLEIGIEEIPASEVKSIVSQINGRLPNLLEQQRINYSNIKTFIASRRFGVLIKG
CCCCHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LPEKQEDYYQEKRGPAEKVAFKDGKPTKALEGFLRSNNATVDDVEVREFKGTSYIFLKKK
CCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEEEC
VEGKKTSLVLSEVFKELIHGLQFKKPMRWGNGEHKFVRPVRWLVALFDDEVLNLELFDKK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
ASDTSYGHRFFFDKIKVTSENYFETLKEALVIADFDERAQMVINELERIEKEKNVTIPRD
CCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AELIDEVTCITELPTAVLGKFLPKYLYLPDEVIIVTIRHHQRTFPVYEGNKLTNSFVAFQ
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEC
DGPADPKGNVRRGYEEVINARLEDAHFYFERDIQKPLEAYVEYLKGILFQRGLGTLYEKT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QRVRSLSKYIAKQLNAKPVEEEAIDRTALLAKADLTTRVVQEFPELQGIMGRIYALESGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
SSEVAWGIQEHYEPLNEVPETLTGAVVGVADRIDTLVGNFLLGNIPSGSKDPYGLRRKLS
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QIFETMYAFGWNMNLIKLFEKAADNLGEGYEKISTTLEPFIKSRYEAFLLEKGYSINIAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
AVSMWWKNPYLGELVAKAISEIIGRDDFDKLLVAYQRVHNISKKHDSNKFDGSLFLKEAE
HHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
VNLFNAYVEALDKIKKALEKRDFSVALKTLIALKEPIDEYFDEVFVMDSQVDIRLNRLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHH
LKALDELFFEIADLSYLINEEHK
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSHKALLEIGIEEIPASEVKSIVSQINGRLPNLLEQQRINYSNIKTFIASRRFGVLIKG
CCCHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LPEKQEDYYQEKRGPAEKVAFKDGKPTKALEGFLRSNNATVDDVEVREFKGTSYIFLKKK
CCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEEEC
VEGKKTSLVLSEVFKELIHGLQFKKPMRWGNGEHKFVRPVRWLVALFDDEVLNLELFDKK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
ASDTSYGHRFFFDKIKVTSENYFETLKEALVIADFDERAQMVINELERIEKEKNVTIPRD
CCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AELIDEVTCITELPTAVLGKFLPKYLYLPDEVIIVTIRHHQRTFPVYEGNKLTNSFVAFQ
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEC
DGPADPKGNVRRGYEEVINARLEDAHFYFERDIQKPLEAYVEYLKGILFQRGLGTLYEKT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QRVRSLSKYIAKQLNAKPVEEEAIDRTALLAKADLTTRVVQEFPELQGIMGRIYALESGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
SSEVAWGIQEHYEPLNEVPETLTGAVVGVADRIDTLVGNFLLGNIPSGSKDPYGLRRKLS
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QIFETMYAFGWNMNLIKLFEKAADNLGEGYEKISTTLEPFIKSRYEAFLLEKGYSINIAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
AVSMWWKNPYLGELVAKAISEIIGRDDFDKLLVAYQRVHNISKKHDSNKFDGSLFLKEAE
HHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
VNLFNAYVEALDKIKKALEKRDFSVALKTLIALKEPIDEYFDEVFVMDSQVDIRLNRLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHH
LKALDELFFEIADLSYLINEEHK
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]