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The map label for this gene is cysK [H]

Identifier: 239618445

GI number: 239618445

Start: 2214168

End: 2215553

Strand: Reverse

Name: cysK [H]

Synonym: Kole_2086

Alternate gene names: 239618445

Gene position: 2215553-2214168 (Counterclockwise)

Preceding gene: 239618446

Following gene: 239618426

Centisome position: 96.24

GC content: 42.57

Gene sequence:

>1386_bases
ATGAAGAAGCTTGTGCTTGGTCCAACATTTGAAGAAATGTTGCATCCCGATAAGATCGACCCAAAAATCAGAAAGAAAGC
ATGGGAAATGAAAAAAGAAGACCCACTCGATCCAATCAATTTTTACAATATTTCCTGGAGAGATATCAACAACAAAGTTT
ATCACGTTGTGCTTCCCAAGGAACTAACAGGTGTGGATGCGAATATAATCGTCCTATATGGTAAGGAATTCCCCACAGGA
AGCCATAAAGTCGGTGCCACATACTCGATACTTATGGAAAAAAGCCTTAGAGGTGAAGTTGATCCGACGTATCACACCCT
CGTTTGGCCATCCACAGGTAATTACGGAATTGGTGGAGCCTGGGTTGGATGTAGAATGAAGTACGATTCAGTGGTTATCC
TACCTGAAGAAATGTCACAAGAGCGCTTCGATATCATAAGAAGTTATGGTGCAAGAGTAATCGCAACACCTGGTTGTGAA
TCCAACGTCAAAGAAATTTATGATAAAGCAAAAGAGCTTAAAGCAGAGAATCCAGAAAAAGTCAGGGTATTGAATCAATT
CGCCGAGTTCGGTAACTATCGTTTCCATTATTATGTCACAGGTAACACGATGATCGAATTGGTAAAAGAACTCGGAATCG
GTAACGGAAAAATTGCTGCTATCGTTGCTGGTGTTGGTTCTGGTGGAACCATCGCTTGTGGTGATAGAGTGAAAGAGGTC
TATCCCGATGCTAAAGTTGTCGCGCTAGAACCTATACAGTGTCCAACGATATCTCTCAACGGTTACGGTGGACACGACAT
CCAGGGAATCGGAGACAAGCATGTTACATGGATTCATAACGTACTGAACAACGATGCTGTTGTTGCAGTCGACGATGTGG
AATCCAAAAAGATGTTGCAGGTTCTAACGGATGAAGAAGGTAAGAAATTCCTAAAGGAATTCCTTCCAGAAAAAACAGTA
GAATTCCTCTCAACAGTCTTCGGTATTTCCGGTGTCGCAAACGTAATTGGTGCTATCAAGATGGCGAAGTACTACGGATT
CACTTCAGAACACAATATCTTCACCATAGCAACCGATAATATTGACCGCTATCATTCCGTCATGGAACAGATGACGAAAA
CCTACGGAAAGCTCGACAGGGGCGAAGCAAAACATCGGTTTGAATCCATATTCCTTGGAGCAAAGACTGATTGGATATTC
GAAGGAACCGTGGAAAACAGAGAAAGATGGCATAATCTGAAGTACTACACCTGGGTTGAACAACAGGGGAAAACGGTTGA
AGAACTAAACGCACAGAGGACTCCGGAATTCTGGCAGAAAGAGAGAGACGAAGTTAAAGTAACCGATGAAATGCTGCTAG
ATTACAGGAAAAAGTACGGAGTGTGA

Upstream 100 bases:

>100_bases
GCCATAGCTAACGGTATTTCACGTGCGATTGGTAAGAGAGTAAAAAGAATACCAGCGACTGCCGAGTATATATTAAAACT
CATCAAGGAGGCATGAACGA

Downstream 100 bases:

>100_bases
TAAAAAAGGCGGCATAAAGCCGCCTTTTTTATTATAGAATAAGCGTTACTTTCTTTGCAGCTTTTAGCCTGTATAGGTTT
TTCGTTCCTCCTGCTCGCGA

Product: Pyridoxal-5'-phosphate-dependent protein beta subunit

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKLVLGPTFEEMLHPDKIDPKIRKKAWEMKKEDPLDPINFYNISWRDINNKVYHVVLPKELTGVDANIIVLYGKEFPTG
SHKVGATYSILMEKSLRGEVDPTYHTLVWPSTGNYGIGGAWVGCRMKYDSVVILPEEMSQERFDIIRSYGARVIATPGCE
SNVKEIYDKAKELKAENPEKVRVLNQFAEFGNYRFHYYVTGNTMIELVKELGIGNGKIAAIVAGVGSGGTIACGDRVKEV
YPDAKVVALEPIQCPTISLNGYGGHDIQGIGDKHVTWIHNVLNNDAVVAVDDVESKKMLQVLTDEEGKKFLKEFLPEKTV
EFLSTVFGISGVANVIGAIKMAKYYGFTSEHNIFTIATDNIDRYHSVMEQMTKTYGKLDRGEAKHRFESIFLGAKTDWIF
EGTVENRERWHNLKYYTWVEQQGKTVEELNAQRTPEFWQKERDEVKVTDEMLLDYRKKYGV

Sequences:

>Translated_461_residues
MKKLVLGPTFEEMLHPDKIDPKIRKKAWEMKKEDPLDPINFYNISWRDINNKVYHVVLPKELTGVDANIIVLYGKEFPTG
SHKVGATYSILMEKSLRGEVDPTYHTLVWPSTGNYGIGGAWVGCRMKYDSVVILPEEMSQERFDIIRSYGARVIATPGCE
SNVKEIYDKAKELKAENPEKVRVLNQFAEFGNYRFHYYVTGNTMIELVKELGIGNGKIAAIVAGVGSGGTIACGDRVKEV
YPDAKVVALEPIQCPTISLNGYGGHDIQGIGDKHVTWIHNVLNNDAVVAVDDVESKKMLQVLTDEEGKKFLKEFLPEKTV
EFLSTVFGISGVANVIGAIKMAKYYGFTSEHNIFTIATDNIDRYHSVMEQMTKTYGKLDRGEAKHRFESIFLGAKTDWIF
EGTVENRERWHNLKYYTWVEQQGKTVEELNAQRTPEFWQKERDEVKVTDEMLLDYRKKYGV
>Mature_461_residues
MKKLVLGPTFEEMLHPDKIDPKIRKKAWEMKKEDPLDPINFYNISWRDINNKVYHVVLPKELTGVDANIIVLYGKEFPTG
SHKVGATYSILMEKSLRGEVDPTYHTLVWPSTGNYGIGGAWVGCRMKYDSVVILPEEMSQERFDIIRSYGARVIATPGCE
SNVKEIYDKAKELKAENPEKVRVLNQFAEFGNYRFHYYVTGNTMIELVKELGIGNGKIAAIVAGVGSGGTIACGDRVKEV
YPDAKVVALEPIQCPTISLNGYGGHDIQGIGDKHVTWIHNVLNNDAVVAVDDVESKKMLQVLTDEEGKKFLKEFLPEKTV
EFLSTVFGISGVANVIGAIKMAKYYGFTSEHNIFTIATDNIDRYHSVMEQMTKTYGKLDRGEAKHRFESIFLGAKTDWIF
EGTVENRERWHNLKYYTWVEQQGKTVEELNAQRTPEFWQKERDEVKVTDEMLLDYRKKYGV

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI295821200, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4557415, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1788754, Length=298, Percent_Identity=30.2013422818792, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI2367138, Length=291, Percent_Identity=26.8041237113402, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535051, Length=267, Percent_Identity=29.9625468164794, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562970, Length=281, Percent_Identity=28.4697508896797, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566674, Length=281, Percent_Identity=28.4697508896797, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115535073, Length=268, Percent_Identity=32.4626865671642, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32566672, Length=211, Percent_Identity=27.4881516587678, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=258, Percent_Identity=29.4573643410853, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24643623, Length=192, Percent_Identity=32.8125, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20129101, Length=192, Percent_Identity=32.8125, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 52435; Mature: 52435

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLGPTFEEMLHPDKIDPKIRKKAWEMKKEDPLDPINFYNISWRDINNKVYHVVLPK
CCCEECCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEECCH
ELTGVDANIIVLYGKEFPTGSHKVGATYSILMEKSLRGEVDPTYHTLVWPSTGNYGIGGA
HHCCCCCEEEEEECCCCCCCCCCCCCHHEEEHHHHHCCCCCCCEEEEECCCCCCCCCCCE
WVGCRMKYDSVVILPEEMSQERFDIIRSYGARVIATPGCESNVKEIYDKAKELKAENPEK
EEEEEEECCCEEEECHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHH
VRVLNQFAEFGNYRFHYYVTGNTMIELVKELGIGNGKIAAIVAGVGSGGTIACGDRVKEV
HHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCHHHHHH
YPDAKVVALEPIQCPTISLNGYGGHDIQGIGDKHVTWIHNVLNNDAVVAVDDVESKKMLQ
CCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
VLTDEEGKKFLKEFLPEKTVEFLSTVFGISGVANVIGAIKMAKYYGFTSEHNIFTIATDN
HHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
IDRYHSVMEQMTKTYGKLDRGEAKHRFESIFLGAKTDWIFEGTVENRERWHNLKYYTWVE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCEEEECCHHHHHHHCCCEEEEEEC
QQGKTVEELNAQRTPEFWQKERDEVKVTDEMLLDYRKKYGV
CCCCCHHHHCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLVLGPTFEEMLHPDKIDPKIRKKAWEMKKEDPLDPINFYNISWRDINNKVYHVVLPK
CCCEECCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEECCH
ELTGVDANIIVLYGKEFPTGSHKVGATYSILMEKSLRGEVDPTYHTLVWPSTGNYGIGGA
HHCCCCCEEEEEECCCCCCCCCCCCCHHEEEHHHHHCCCCCCCEEEEECCCCCCCCCCCE
WVGCRMKYDSVVILPEEMSQERFDIIRSYGARVIATPGCESNVKEIYDKAKELKAENPEK
EEEEEEECCCEEEECHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHH
VRVLNQFAEFGNYRFHYYVTGNTMIELVKELGIGNGKIAAIVAGVGSGGTIACGDRVKEV
HHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCHHHHHH
YPDAKVVALEPIQCPTISLNGYGGHDIQGIGDKHVTWIHNVLNNDAVVAVDDVESKKMLQ
CCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
VLTDEEGKKFLKEFLPEKTVEFLSTVFGISGVANVIGAIKMAKYYGFTSEHNIFTIATDN
HHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
IDRYHSVMEQMTKTYGKLDRGEAKHRFESIFLGAKTDWIFEGTVENRERWHNLKYYTWVE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCEEEECCHHHHHHHCCCEEEEEEC
QQGKTVEELNAQRTPEFWQKERDEVKVTDEMLLDYRKKYGV
CCCCCHHHHCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA