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The map label for this gene is tvaII [H]

Identifier: 239618369

GI number: 239618369

Start: 2132364

End: 2134355

Strand: Reverse

Name: tvaII [H]

Synonym: Kole_2007

Alternate gene names: 239618369

Gene position: 2134355-2132364 (Counterclockwise)

Preceding gene: 239618371

Following gene: 239618367

Centisome position: 92.71

GC content: 42.47

Gene sequence:

>1992_bases
ATGAGGAAAGCTTTACTTTTGTTGTTTATCATGTTGGCTTGTTTAGGTTTCGCAGCGGATTTCTTTATCGAGTCAGACCA
GCAGGTGTTTTTAATAGGTGATTTCACCGAGTGGGAACCGGTTCCTCTGGAAAAGGCTGCAGGCAGTTGGTGGTATCTGT
CAGTGAGCTTGGACAACGGGACCTATAATTACTATTTCACTGATGAATCTGGTAACCGCCTTATTGATCCTTTCAAAGAG
ACAACAAACATTGAGGGTAAAACCTTCAACATTTTCAGGGTTGAAGATCTTGAACCCGCAACCCACAAAATATGGGATAG
AGAATATTTTAATCCCGTGAAGCCGGGAGAATTTTACTTGAGCGTCTCAGGAAAAGAAGAAGCTTTCACTAAAGCGTTCA
TCCACATAAATGGCGCAAAATTAGAAATGCCGTTTTTGAAAAACTCCGGAAATCAGGACTATTTCAGAATCCATCTAACT
GATTTGCAGAACCTCGAATACTATTTTGAACTGCTCGGCAACGATAAAAAGCTATTTTTGGGCGCCAACGGCGTTTCAGA
ACAATCCGTTATTCCTTTCAGATTCACCCCAGACAATCTTCCTGTAAATTATTTCGACACACCAGAATGGTCGAAAGGAG
CAGTGTATTACCAGATTTTCCCTGAAAGGTTCGCAAACGGTGACCCTTCGAATGATCCCGAGGGTTCACAAAATTGGTAC
GCAGATCCCAAAAGCGCCAACCTTGGTTCCGATGGTTTCTTTGGCGGAGATCTTCAGGGTGTTATCGATCACATGGATCA
CCTGAAAGACCTTGGGATAGATGCCATCTACTTTAACCCTATTTTTGAGAGTGTCTCGAGTCACAAGTACGATACCGCGG
ATTATATGAAAATCGATGACAATTTTGGGGATTACGAACTCTTCAAGAAAATGGTGAATGATCTATCTTCATCCGGGATA
AGGGTAATCCTGGACGGAGTATTCAACCACACTGGCGACGAATTCAGGGCATTCCAGGATGTTAAGAAAAACGGCAAAGA
TTCCCCCTACTGGGATTGGTATTTTATAAAAGGAAATAAACCGAGAAGGTACAAGGGACACGCCATGAACTACATCGCCT
GGGGCGGCTACGCCGATATGCCGAAATTGAACGTTCTAAATCCAGAGGTACAGGAATACATCGGCAAGGTAGCTGAAAAA
TACGCCAAAGCAGGCATTTCAGGCTGGAGACTTGATGTTGCTGGAGAAGTCGCTCCAGAATTCTGGAAGAACTTTTTCCG
CCCCACTGTCAAAAGTATGAACAAAGAATCTATAATCGTTGGGGAAATCTGGGGAGACTCAAAGGTGTATCTGCAAGGAA
ACATGTTTGACTCTGTTATGAATTACCAGTTCAGGGATGCAGTTATCGAGTATGTTGCAAGACCGATGCATTCCGCGAAA
AAATTCGCCAACATGACTGGGTTTTATCTCAAAAGATATCCACCCCAGGTTCTGCATTCTCTATGGAATATGCTGGATAG
TCACGATACTGAAAGAATGCTTACAACACTTTATGGTGACATAGAGCTTTTCAAAATAGCCGTAGGCTTGCAGATGACTT
TTATAGGTTCCCCGGTGATCTACTATGGAGACGAAATAGGCATGACAGGAGGAAAAGATCCTGATAATCGAAGACCTATG
CCGTGGAAAGAAGAACTCTGGAATAAGGATATCCTCGAATACTATAAAAAACTCATTAGCTTCAGAAAGGAATATCCTGC
TCTGAGGAAAGGTTCCTTCGAGATCATCGCCACTGATGGAACTTTGATGGCTTATAAAAGAACGTTAGAAGATGAGGAAA
TCATCATTTTTGCTAATCCTGGAAACGAAAAGGCAACCTTTTCCGGATCACTACCAGGTCTTTACCATGAACTATTTACC
AATACCCAGATGGAGATCAAAAACTTAGAAGTTCCAGCTAAAAGTTTTCTCATTTTCAAGCGGATACGCTGA

Upstream 100 bases:

>100_bases
CATAACTAAAATACAAAAGGAAAGATTTGTTTTGCTGAGTCCCCAGAAACTGTTATGATACTAATAGACAGCTAAGCTTA
AAAAGTAGGGAGATGTTTGG

Downstream 100 bases:

>100_bases
TATTTTCTAAAAAGAATTTCGTGCCTGACATATCATATAAGGATGACCTGGAGCAATTCTGCGATCGAGTGATTCAGATC
GGTTGAAAATTTTTATTGCT

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: Alpha-amylase II; TVA II [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MRKALLLLFIMLACLGFAADFFIESDQQVFLIGDFTEWEPVPLEKAAGSWWYLSVSLDNGTYNYYFTDESGNRLIDPFKE
TTNIEGKTFNIFRVEDLEPATHKIWDREYFNPVKPGEFYLSVSGKEEAFTKAFIHINGAKLEMPFLKNSGNQDYFRIHLT
DLQNLEYYFELLGNDKKLFLGANGVSEQSVIPFRFTPDNLPVNYFDTPEWSKGAVYYQIFPERFANGDPSNDPEGSQNWY
ADPKSANLGSDGFFGGDLQGVIDHMDHLKDLGIDAIYFNPIFESVSSHKYDTADYMKIDDNFGDYELFKKMVNDLSSSGI
RVILDGVFNHTGDEFRAFQDVKKNGKDSPYWDWYFIKGNKPRRYKGHAMNYIAWGGYADMPKLNVLNPEVQEYIGKVAEK
YAKAGISGWRLDVAGEVAPEFWKNFFRPTVKSMNKESIIVGEIWGDSKVYLQGNMFDSVMNYQFRDAVIEYVARPMHSAK
KFANMTGFYLKRYPPQVLHSLWNMLDSHDTERMLTTLYGDIELFKIAVGLQMTFIGSPVIYYGDEIGMTGGKDPDNRRPM
PWKEELWNKDILEYYKKLISFRKEYPALRKGSFEIIATDGTLMAYKRTLEDEEIIIFANPGNEKATFSGSLPGLYHELFT
NTQMEIKNLEVPAKSFLIFKRIR

Sequences:

>Translated_663_residues
MRKALLLLFIMLACLGFAADFFIESDQQVFLIGDFTEWEPVPLEKAAGSWWYLSVSLDNGTYNYYFTDESGNRLIDPFKE
TTNIEGKTFNIFRVEDLEPATHKIWDREYFNPVKPGEFYLSVSGKEEAFTKAFIHINGAKLEMPFLKNSGNQDYFRIHLT
DLQNLEYYFELLGNDKKLFLGANGVSEQSVIPFRFTPDNLPVNYFDTPEWSKGAVYYQIFPERFANGDPSNDPEGSQNWY
ADPKSANLGSDGFFGGDLQGVIDHMDHLKDLGIDAIYFNPIFESVSSHKYDTADYMKIDDNFGDYELFKKMVNDLSSSGI
RVILDGVFNHTGDEFRAFQDVKKNGKDSPYWDWYFIKGNKPRRYKGHAMNYIAWGGYADMPKLNVLNPEVQEYIGKVAEK
YAKAGISGWRLDVAGEVAPEFWKNFFRPTVKSMNKESIIVGEIWGDSKVYLQGNMFDSVMNYQFRDAVIEYVARPMHSAK
KFANMTGFYLKRYPPQVLHSLWNMLDSHDTERMLTTLYGDIELFKIAVGLQMTFIGSPVIYYGDEIGMTGGKDPDNRRPM
PWKEELWNKDILEYYKKLISFRKEYPALRKGSFEIIATDGTLMAYKRTLEDEEIIIFANPGNEKATFSGSLPGLYHELFT
NTQMEIKNLEVPAKSFLIFKRIR
>Mature_663_residues
MRKALLLLFIMLACLGFAADFFIESDQQVFLIGDFTEWEPVPLEKAAGSWWYLSVSLDNGTYNYYFTDESGNRLIDPFKE
TTNIEGKTFNIFRVEDLEPATHKIWDREYFNPVKPGEFYLSVSGKEEAFTKAFIHINGAKLEMPFLKNSGNQDYFRIHLT
DLQNLEYYFELLGNDKKLFLGANGVSEQSVIPFRFTPDNLPVNYFDTPEWSKGAVYYQIFPERFANGDPSNDPEGSQNWY
ADPKSANLGSDGFFGGDLQGVIDHMDHLKDLGIDAIYFNPIFESVSSHKYDTADYMKIDDNFGDYELFKKMVNDLSSSGI
RVILDGVFNHTGDEFRAFQDVKKNGKDSPYWDWYFIKGNKPRRYKGHAMNYIAWGGYADMPKLNVLNPEVQEYIGKVAEK
YAKAGISGWRLDVAGEVAPEFWKNFFRPTVKSMNKESIIVGEIWGDSKVYLQGNMFDSVMNYQFRDAVIEYVARPMHSAK
KFANMTGFYLKRYPPQVLHSLWNMLDSHDTERMLTTLYGDIELFKIAVGLQMTFIGSPVIYYGDEIGMTGGKDPDNRRPM
PWKEELWNKDILEYYKKLISFRKEYPALRKGSFEIIATDGTLMAYKRTLEDEEIIIFANPGNEKATFSGSLPGLYHELFT
NTQMEIKNLEVPAKSFLIFKRIR

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=223, Percent_Identity=24.6636771300448, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1786604, Length=500, Percent_Identity=31.2, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1790687, Length=549, Percent_Identity=24.0437158469945, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789995, Length=203, Percent_Identity=26.1083743842365, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322245, Length=420, Percent_Identity=24.047619047619, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322241, Length=231, Percent_Identity=25.1082251082251, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=231, Percent_Identity=25.1082251082251, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=417, Percent_Identity=22.0623501199041, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=417, Percent_Identity=22.0623501199041, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=231, Percent_Identity=25.1082251082251, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586599, Length=529, Percent_Identity=22.4952741020794, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45549022, Length=570, Percent_Identity=22.9824561403509, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24586593, Length=562, Percent_Identity=22.4199288256228, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=27.5555555555556, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330053, Length=237, Percent_Identity=26.5822784810127, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586587, Length=428, Percent_Identity=23.3644859813084, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586597, Length=584, Percent_Identity=21.5753424657534, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586589, Length=521, Percent_Identity=23.0326295585413, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=25.3275109170306, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=25.3275109170306, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583745, Length=223, Percent_Identity=25.1121076233184, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 76514; Mature: 76514

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKALLLLFIMLACLGFAADFFIESDQQVFLIGDFTEWEPVPLEKAAGSWWYLSVSLDNG
CCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEEECCC
TYNYYFTDESGNRLIDPFKETTNIEGKTFNIFRVEDLEPATHKIWDREYFNPVKPGEFYL
EEEEEEECCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
SVSGKEEAFTKAFIHINGAKLEMPFLKNSGNQDYFRIHLTDLQNLEYYFELLGNDKKLFL
EECCCCCCEEEEEEEECCCEEEECHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEE
GANGVSEQSVIPFRFTPDNLPVNYFDTPEWSKGAVYYQIFPERFANGDPSNDPEGSQNWY
ECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCC
ADPKSANLGSDGFFGGDLQGVIDHMDHLKDLGIDAIYFNPIFESVSSHKYDTADYMKIDD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCEEEECC
NFGDYELFKKMVNDLSSSGIRVILDGVFNHTGDEFRAFQDVKKNGKDSPYWDWYFIKGNK
CCCHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
PRRYKGHAMNYIAWGGYADMPKLNVLNPEVQEYIGKVAEKYAKAGISGWRLDVAGEVAPE
CCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
FWKNFFRPTVKSMNKESIIVGEIWGDSKVYLQGNMFDSVMNYQFRDAVIEYVARPMHSAK
HHHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHH
KFANMTGFYLKRYPPQVLHSLWNMLDSHDTERMLTTLYGDIELFKIAVGLQMTFIGSPVI
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECEEEEEECCCEE
YYGDEIGMTGGKDPDNRRPMPWKEELWNKDILEYYKKLISFRKEYPALRKGSFEIIATDG
EECCEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECC
TLMAYKRTLEDEEIIIFANPGNEKATFSGSLPGLYHELFTNTQMEIKNLEVPAKSFLIFK
EEEEHHHCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHEEE
RIR
ECC
>Mature Secondary Structure
MRKALLLLFIMLACLGFAADFFIESDQQVFLIGDFTEWEPVPLEKAAGSWWYLSVSLDNG
CCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCHHHCCCCEEEEEEEECCC
TYNYYFTDESGNRLIDPFKETTNIEGKTFNIFRVEDLEPATHKIWDREYFNPVKPGEFYL
EEEEEEECCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
SVSGKEEAFTKAFIHINGAKLEMPFLKNSGNQDYFRIHLTDLQNLEYYFELLGNDKKLFL
EECCCCCCEEEEEEEECCCEEEECHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEE
GANGVSEQSVIPFRFTPDNLPVNYFDTPEWSKGAVYYQIFPERFANGDPSNDPEGSQNWY
ECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCC
ADPKSANLGSDGFFGGDLQGVIDHMDHLKDLGIDAIYFNPIFESVSSHKYDTADYMKIDD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCEEEECC
NFGDYELFKKMVNDLSSSGIRVILDGVFNHTGDEFRAFQDVKKNGKDSPYWDWYFIKGNK
CCCHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
PRRYKGHAMNYIAWGGYADMPKLNVLNPEVQEYIGKVAEKYAKAGISGWRLDVAGEVAPE
CCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
FWKNFFRPTVKSMNKESIIVGEIWGDSKVYLQGNMFDSVMNYQFRDAVIEYVARPMHSAK
HHHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHH
KFANMTGFYLKRYPPQVLHSLWNMLDSHDTERMLTTLYGDIELFKIAVGLQMTFIGSPVI
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECEEEEEECCCEE
YYGDEIGMTGGKDPDNRRPMPWKEELWNKDILEYYKKLISFRKEYPALRKGSFEIIATDG
EECCEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECC
TLMAYKRTLEDEEIIIFANPGNEKATFSGSLPGLYHELFTNTQMEIKNLEVPAKSFLIFK
EEEEHHHCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHEEE
RIR
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763540; 10222200; 12051850 [H]