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The map label for this gene is rlmN [H]

Identifier: 239618306

GI number: 239618306

Start: 2062153

End: 2063184

Strand: Reverse

Name: rlmN [H]

Synonym: Kole_1943

Alternate gene names: 239618306

Gene position: 2063184-2062153 (Counterclockwise)

Preceding gene: 239618307

Following gene: 239618305

Centisome position: 89.62

GC content: 40.31

Gene sequence:

>1032_bases
ATGCAGTTGCTGGAGATGACACTGGATGAACTAAGAAAAGTTCTTGTGGATGAAGGTTATGAAAAATATAGAGCTTCTCA
GATATTCGATTGGATCTATAAAAAGAAGGTTCTCAATTTCTCTAATATGACGAACCTTCCGAAAGATTTCAGGAAATTTC
TGTCTGGAACCTTTCGATATCCTGAAATGACAATAGTTCGAAGAAGTCTTTCAAAAATAGACGGCACTGAAAAATTCCTT
TGGAAGTTGCATGACGGTGAGTTTATAGAATCGGTAATACTGAGACATCCTGATCATACAACCTTTTGTATTTCCACACA
GGTTGGTTGTCAGCTCGGCTGCATCTTTTGTGCCACAGGAATGAGTGGCTTTAAACGTAATCTCAGCGTCTCAGAAATAG
TGGGGCAGGTCATCTTTATGGAAAAATCCATGGGAAAAAATGTTACCAACATCGTATTCATGGGGATGGGAGAACCTTTC
TTAAATACTGACAACGTTTTTAAATCCATTGAAATCCTGCATGAGCCCGCGGGAAGAAACCTTGGAATACGGCACTTCAC
CATCTCAACTGCTGGCATCCCGGAAGGAATCATAAGACTCGCTGATTCAGGAATGGACGTAAGATTATCACTTTCACTGC
ATGCAGCCACAGATGAAAAGAGAAGCATGTTGATGCCGATAAACAAACGATACAACATTCAGCAGCTCATGGCGTCTCTT
GAATATTATCAGAGGAAAACAAATCGGAGGATCACCATTGAATATATCCTCATCGATGGTATAAATGATTCAATAGAAGA
CGCGAAACAGCTGGCAAAATTATTTAAGCACCTCAAGATATTCGTGAACATCATCGCGATTAATCCCGTTGTTCCAACCC
TAAAAAGACCTTCCAGAGAAAAAGTGGAACGTTTTGCGATAGAACTGAAAAAGCACGGAATCGAAGCTGCAATCAGGACA
GAAAAAGGAAGTGACATAGATGCAGCATGTGGTCAATTAAGAAGAAAAAATCTTCAGGAGGAGAGAGCTTGA

Upstream 100 bases:

>100_bases
TATAACCTGCGGGGTACGTGGGATGGATACGGTTACTGGCTTTTGCCTCCCAATAGCCCAAGACCTGGATACATCGCTAG
AATGAGGAGGAAATAAAGGG

Downstream 100 bases:

>100_bases
CTGAAAAAAGGAAAAAGGGTGTAGTTATCAGATTTGGTAGTAGAACAATGGTTGTGGCTGACTTGCATACCAAAAAGAGG
TACATCTGCAACATGCCAGG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MQLLEMTLDELRKVLVDEGYEKYRASQIFDWIYKKKVLNFSNMTNLPKDFRKFLSGTFRYPEMTIVRRSLSKIDGTEKFL
WKLHDGEFIESVILRHPDHTTFCISTQVGCQLGCIFCATGMSGFKRNLSVSEIVGQVIFMEKSMGKNVTNIVFMGMGEPF
LNTDNVFKSIEILHEPAGRNLGIRHFTISTAGIPEGIIRLADSGMDVRLSLSLHAATDEKRSMLMPINKRYNIQQLMASL
EYYQRKTNRRITIEYILIDGINDSIEDAKQLAKLFKHLKIFVNIIAINPVVPTLKRPSREKVERFAIELKKHGIEAAIRT
EKGSDIDAACGQLRRKNLQEERA

Sequences:

>Translated_343_residues
MQLLEMTLDELRKVLVDEGYEKYRASQIFDWIYKKKVLNFSNMTNLPKDFRKFLSGTFRYPEMTIVRRSLSKIDGTEKFL
WKLHDGEFIESVILRHPDHTTFCISTQVGCQLGCIFCATGMSGFKRNLSVSEIVGQVIFMEKSMGKNVTNIVFMGMGEPF
LNTDNVFKSIEILHEPAGRNLGIRHFTISTAGIPEGIIRLADSGMDVRLSLSLHAATDEKRSMLMPINKRYNIQQLMASL
EYYQRKTNRRITIEYILIDGINDSIEDAKQLAKLFKHLKIFVNIIAINPVVPTLKRPSREKVERFAIELKKHGIEAAIRT
EKGSDIDAACGQLRRKNLQEERA
>Mature_343_residues
MQLLEMTLDELRKVLVDEGYEKYRASQIFDWIYKKKVLNFSNMTNLPKDFRKFLSGTFRYPEMTIVRRSLSKIDGTEKFL
WKLHDGEFIESVILRHPDHTTFCISTQVGCQLGCIFCATGMSGFKRNLSVSEIVGQVIFMEKSMGKNVTNIVFMGMGEPF
LNTDNVFKSIEILHEPAGRNLGIRHFTISTAGIPEGIIRLADSGMDVRLSLSLHAATDEKRSMLMPINKRYNIQQLMASL
EYYQRKTNRRITIEYILIDGINDSIEDAKQLAKLFKHLKIFVNIIAINPVVPTLKRPSREKVERFAIELKKHGIEAAIRT
EKGSDIDAACGQLRRKNLQEERA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=346, Percent_Identity=39.5953757225434, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39358; Mature: 39358

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLEMTLDELRKVLVDEGYEKYRASQIFDWIYKKKVLNFSNMTNLPKDFRKFLSGTFRY
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCC
PEMTIVRRSLSKIDGTEKFLWKLHDGEFIESVILRHPDHTTFCISTQVGCQLGCIFCATG
CHHHHHHHHHHHCCCCHHHHEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCC
MSGFKRNLSVSEIVGQVIFMEKSMGKNVTNIVFMGMGEPFLNTDNVFKSIEILHEPAGRN
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
LGIRHFTISTAGIPEGIIRLADSGMDVRLSLSLHAATDEKRSMLMPINKRYNIQQLMASL
CCEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCEECCCCCCCCHHHHHHHH
EYYQRKTNRRITIEYILIDGINDSIEDAKQLAKLFKHLKIFVNIIAINPVVPTLKRPSRE
HHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHH
KVERFAIELKKHGIEAAIRTEKGSDIDAACGQLRRKNLQEERA
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLLEMTLDELRKVLVDEGYEKYRASQIFDWIYKKKVLNFSNMTNLPKDFRKFLSGTFRY
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCC
PEMTIVRRSLSKIDGTEKFLWKLHDGEFIESVILRHPDHTTFCISTQVGCQLGCIFCATG
CHHHHHHHHHHHCCCCHHHHEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCC
MSGFKRNLSVSEIVGQVIFMEKSMGKNVTNIVFMGMGEPFLNTDNVFKSIEILHEPAGRN
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
LGIRHFTISTAGIPEGIIRLADSGMDVRLSLSLHAATDEKRSMLMPINKRYNIQQLMASL
CCEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCEECCCCCCCCHHHHHHHH
EYYQRKTNRRITIEYILIDGINDSIEDAKQLAKLFKHLKIFVNIIAINPVVPTLKRPSRE
HHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHH
KVERFAIELKKHGIEAAIRTEKGSDIDAACGQLRRKNLQEERA
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA