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The map label for this gene is tig

Identifier: 239618301

GI number: 239618301

Start: 2057798

End: 2059117

Strand: Direct

Name: tig

Synonym: Kole_1938

Alternate gene names: 239618301

Gene position: 2057798-2059117 (Clockwise)

Preceding gene: 239618300

Following gene: 239618302

Centisome position: 89.39

GC content: 40.38

Gene sequence:

>1320_bases
GTGGAGAAAAATGTTGTTAAGGCAGAAAAGAACGTGGAAATAGTTGAATTTACCTTTGGACCGGATGAGATCGCTGAAGC
GGAAAATGAGATAGTCAGGTATGTAAACAAGAACTACACGATACCAGGATTCAGAAAGGGTAAAGCTCCGAAGAGAATAA
TTGAGACCTTTTTCGGTGAAAATTTCAGAGATATGGTTCTGGAAGAACTTTCCAGGAAGATTGAGGATACTCTCAAAGAT
GAAGAACTTTTTATTCCTGCTGTCATTGCTGACCGAAAGATCGAAGGTGATGTTGCTGTCTTCGTAGTTGAACTTCACCG
CGAGCCAAAGGTTGAACTCAAGGATTACACTGGCCTGGAGTTGTCCGTTCCCAAGCAGGAAGAGGTTCTCGCCAATTACG
TGGATAACAAACTCGAGGAGCTTAGAAACGAAGCGGCTATAGTTGAACCAAAGGACGGGCCAGCTGAAATTGGTGATGTT
ATTAACATTGAGTACACGATAGAAAAGGATGGCAAGATAATTGCCGATCATAAAACACAGGAAATACTGATCGTTGAAGA
TGATGACAGACCCATTGTTACGAACGTAATTGGAAAGAAAAAGGGAGATATCGTTGAATTTGACAGAACCTTTGAAAACT
CAAACAACAAATACCATTACAAGATAGAAATTAAGGAAGTCCTCAAGAGAACGCTTATGGAACTTAATGATGAATTTGCC
AAAAGTGTTGCCTCTGAAGTGAACACCCTGGAGGAACTGAAGAAGAAACTGGAAGAGGAAGGGCTCGAGGCTTTCGAAAG
CTGGAAGAAAGATTTCCTGCGCCAGCAGGTACAGGATAAATTAGCAGAGCTTGTTGAACTTGAGATTTCTGAAAAAACAC
TGGACTATTTTGTCAATAGGGCAATAGAAAACGCAAAGAAAGAGAACACTTATGATTCTTATCTGAAGCAGGCTGGCAGT
GAAGAAAAGCTTTATGAAGAATTCAAAACGGGTATTCTCAACGAATTGAAAAAGAACACAGCCATTGAGAAGATAGCAGA
AAAAGAACAAATCGAAGTAACCGATGAAGAGGTTATGAAGACCGCAGAAGAACTTTCGACCTATTGGGGAATTTCACCGG
AACGTGCAAAAGAGATTGTCAAAACCAGAGAAGATATTAGAAACGATATTGTAGAGAACATACGTCGAACGAAAGTACAG
GACCTTATCGTGGAAAAAGCCACAATAAAAGAGATTAGTGCTGATGAACCCGTTGAAGAACAGAAAGAGGAAGAAGAAAA
GGAAGAAGCAGGTTCGGAGAATTCAGAAAACAAAGAGTAA

Upstream 100 bases:

>100_bases
TGAACTCGTGGAATTATAACCTTCGTTTAACCGATAGAATAGAATTGAAACGTATAATCGACAGGCAAGAAAGCTGAAAA
AAAGATAGGAGGGTTTCTTT

Downstream 100 bases:

>100_bases
GAGATAAAGAATATAAACTGCGTGCGGAAAACCGCACGCAGTTTTAATGGGGGGATTTTTTTGAAAAAGAGGAGAATTAT
TCTTGGCGTATTTTGTGCGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MEKNVVKAEKNVEIVEFTFGPDEIAEAENEIVRYVNKNYTIPGFRKGKAPKRIIETFFGENFRDMVLEELSRKIEDTLKD
EELFIPAVIADRKIEGDVAVFVVELHREPKVELKDYTGLELSVPKQEEVLANYVDNKLEELRNEAAIVEPKDGPAEIGDV
INIEYTIEKDGKIIADHKTQEILIVEDDDRPIVTNVIGKKKGDIVEFDRTFENSNNKYHYKIEIKEVLKRTLMELNDEFA
KSVASEVNTLEELKKKLEEEGLEAFESWKKDFLRQQVQDKLAELVELEISEKTLDYFVNRAIENAKKENTYDSYLKQAGS
EEKLYEEFKTGILNELKKNTAIEKIAEKEQIEVTDEEVMKTAEELSTYWGISPERAKEIVKTREDIRNDIVENIRRTKVQ
DLIVEKATIKEISADEPVEEQKEEEEKEEAGSENSENKE

Sequences:

>Translated_439_residues
MEKNVVKAEKNVEIVEFTFGPDEIAEAENEIVRYVNKNYTIPGFRKGKAPKRIIETFFGENFRDMVLEELSRKIEDTLKD
EELFIPAVIADRKIEGDVAVFVVELHREPKVELKDYTGLELSVPKQEEVLANYVDNKLEELRNEAAIVEPKDGPAEIGDV
INIEYTIEKDGKIIADHKTQEILIVEDDDRPIVTNVIGKKKGDIVEFDRTFENSNNKYHYKIEIKEVLKRTLMELNDEFA
KSVASEVNTLEELKKKLEEEGLEAFESWKKDFLRQQVQDKLAELVELEISEKTLDYFVNRAIENAKKENTYDSYLKQAGS
EEKLYEEFKTGILNELKKNTAIEKIAEKEQIEVTDEEVMKTAEELSTYWGISPERAKEIVKTREDIRNDIVENIRRTKVQ
DLIVEKATIKEISADEPVEEQKEEEEKEEAGSENSENKE
>Mature_439_residues
MEKNVVKAEKNVEIVEFTFGPDEIAEAENEIVRYVNKNYTIPGFRKGKAPKRIIETFFGENFRDMVLEELSRKIEDTLKD
EELFIPAVIADRKIEGDVAVFVVELHREPKVELKDYTGLELSVPKQEEVLANYVDNKLEELRNEAAIVEPKDGPAEIGDV
INIEYTIEKDGKIIADHKTQEILIVEDDDRPIVTNVIGKKKGDIVEFDRTFENSNNKYHYKIEIKEVLKRTLMELNDEFA
KSVASEVNTLEELKKKLEEEGLEAFESWKKDFLRQQVQDKLAELVELEISEKTLDYFVNRAIENAKKENTYDSYLKQAGS
EEKLYEEFKTGILNELKKNTAIEKIAEKEQIEVTDEEVMKTAEELSTYWGISPERAKEIVKTREDIRNDIVENIRRTKVQ
DLIVEKATIKEISADEPVEEQKEEEEKEEAGSENSENKE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=295, Percent_Identity=27.4576271186441, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_KOSOT (C5CGV0)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941623.1
- GeneID:   7968429
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1938
- ProtClustDB:   CLSK875303
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 50878; Mature: 50878

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNVVKAEKNVEIVEFTFGPDEIAEAENEIVRYVNKNYTIPGFRKGKAPKRIIETFFGE
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC
NFRDMVLEELSRKIEDTLKDEELFIPAVIADRKIEGDVAVFVVELHREPKVELKDYTGLE
CHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCE
LSVPKQEEVLANYVDNKLEELRNEAAIVEPKDGPAEIGDVINIEYTIEKDGKIIADHKTQ
EECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCEEEEEEEEECCCEEEECCCCC
EILIVEDDDRPIVTNVIGKKKGDIVEFDRTFENSNNKYHYKIEIKEVLKRTLMELNDEFA
EEEEEECCCCCHHHHHHCCCCCCEEHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
KSVASEVNTLEELKKKLEEEGLEAFESWKKDFLRQQVQDKLAELVELEISEKTLDYFVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIENAKKENTYDSYLKQAGSEEKLYEEFKTGILNELKKNTAIEKIAEKEQIEVTDEEVMK
HHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TAEELSTYWGISPERAKEIVKTREDIRNDIVENIRRTKVQDLIVEKATIKEISADEPVEE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QKEEEEKEEAGSENSENKE
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKNVVKAEKNVEIVEFTFGPDEIAEAENEIVRYVNKNYTIPGFRKGKAPKRIIETFFGE
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC
NFRDMVLEELSRKIEDTLKDEELFIPAVIADRKIEGDVAVFVVELHREPKVELKDYTGLE
CHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCE
LSVPKQEEVLANYVDNKLEELRNEAAIVEPKDGPAEIGDVINIEYTIEKDGKIIADHKTQ
EECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCEEEEEEEEECCCEEEECCCCC
EILIVEDDDRPIVTNVIGKKKGDIVEFDRTFENSNNKYHYKIEIKEVLKRTLMELNDEFA
EEEEEECCCCCHHHHHHCCCCCCEEHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHH
KSVASEVNTLEELKKKLEEEGLEAFESWKKDFLRQQVQDKLAELVELEISEKTLDYFVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIENAKKENTYDSYLKQAGSEEKLYEEFKTGILNELKKNTAIEKIAEKEQIEVTDEEVMK
HHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TAEELSTYWGISPERAKEIVKTREDIRNDIVENIRRTKVQDLIVEKATIKEISADEPVEE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QKEEEEKEEAGSENSENKE
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA