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The map label for this gene is tuf

Identifier: 239618267

GI number: 239618267

Start: 2024529

End: 2025728

Strand: Reverse

Name: tuf

Synonym: Kole_1904

Alternate gene names: 239618267

Gene position: 2025728-2024529 (Counterclockwise)

Preceding gene: 239618268

Following gene: 239618266

Centisome position: 87.99

GC content: 45.92

Gene sequence:

>1200_bases
ATGGCTAAGGAAAAATTTGAACGAACAAAACCACATCTTAACATTGGTACAATCGGACACATTGACCACGGTAAAACCAC
ATTGACAGCAGCCATAACAAAAGCTCTCGCCTATAAGGGGTTTGCTGATTTCACACCATTCGATGCTATCGACAAGGCTC
CTGAAGAAAAGGCCAGAGGTATCACAATCAACGTTACTCATGTTGAGTATGAAACCGAAAAGAGACATTACGCCCACATT
GACTGTCCTGGGCACGCCGACTACATTAAGAACATGATCACCGGTGCTGCTCAGATGGATGGTGCTATTCTTGTTGTTGC
AGCTACCGACGGTGTCATGCCACAGACCAGAGAGCATGTTCTTCTCGCTAGACAGGTTAATGTTCCTGCCATGGTCGTCT
TCATAAACAAAGTTGACATGGTTGACGACGAAGAACTCGTTGAACTCGTTGAAGAAGAAGTTAGAGACCTTCTCAGCAAA
TACGAGTTCCCTGGTGACGAGGTCCCTGTTATAAAAGGATCTGCTCTCATGGCTCTTGAAGCTGATAATCCTGATGACCC
ATGGGTCCAGAAAATCTACGAACTCATGGACGCTGTTGACAATTACGTTCCTGAGCCCCAGAGAGAAACCGACAAACCAT
TCCTCATGCCTATCGAAGATATCTTCTCCATCACCGGAAGAGGTACAGTTGTTACCGGAAGAATCGAACGTGGCGTAATC
CATGTCGGTGATGAAGTTGAAATAGTTGGACTTTCCTACGAGGTTAGAAAAACGGTTGTTACCGGTGTTGAAATGTTCAG
AAAACTCCTCGACGAAGGTGTTGCAGGTGACAACGTTGGATGTCTGCTCAGAGGCGTTGGTAAAGACGAGGTCAAAAGAG
GTCAGGTTCTTGCAAAACCTGGTTCAATCACTCCACATAAGAAATTCAAGGCTAACATTTACGTTTTGAAGAAAGAAGAA
GGCGGAAGGCATACTCCTTTCACCAAAGGATACAGACCGCAGTTCTACATCAGAACGGCTGACGTCACTGGTGAACTCGT
TGATCTCCCAGAGGGTGTCGAAATGGTTATGCCTGGTGATAACGTCGTTATGACAGTTGAGCTCATTTACCCTGTCGCTA
TCGAAAAAGGTATGAGATTTGCTGTTCGTGAAGGCGGAAGAACCGTCGGCGCTGGTGTTGTCAGCGAAATTATCGAGTAA

Upstream 100 bases:

>100_bases
ACGTAATCCAGTTCTCGCATTACGCTGAAGTACCGGAAAAAATAGTGAAAAAAATCTTTGGAGAATAAACCTATAACCAC
TTCCAAAAGGAGGGAAAGAA

Downstream 100 bases:

>100_bases
TCTAAGTTGAGGGGAGGGAACTTCCCTCCCTTTATCTGTGAAGGAGGGAATCAAAAGGCATGGCCAAACAAAAAATCAGG
ATTCGCCTCAAGGCTTATGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKALAYKGFADFTPFDAIDKAPEEKARGITINVTHVEYETEKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAATDGVMPQTREHVLLARQVNVPAMVVFINKVDMVDDEELVELVEEEVRDLLSK
YEFPGDEVPVIKGSALMALEADNPDDPWVQKIYELMDAVDNYVPEPQRETDKPFLMPIEDIFSITGRGTVVTGRIERGVI
HVGDEVEIVGLSYEVRKTVVTGVEMFRKLLDEGVAGDNVGCLLRGVGKDEVKRGQVLAKPGSITPHKKFKANIYVLKKEE
GGRHTPFTKGYRPQFYIRTADVTGELVDLPEGVEMVMPGDNVVMTVELIYPVAIEKGMRFAVREGGRTVGAGVVSEIIE

Sequences:

>Translated_399_residues
MAKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKALAYKGFADFTPFDAIDKAPEEKARGITINVTHVEYETEKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAATDGVMPQTREHVLLARQVNVPAMVVFINKVDMVDDEELVELVEEEVRDLLSK
YEFPGDEVPVIKGSALMALEADNPDDPWVQKIYELMDAVDNYVPEPQRETDKPFLMPIEDIFSITGRGTVVTGRIERGVI
HVGDEVEIVGLSYEVRKTVVTGVEMFRKLLDEGVAGDNVGCLLRGVGKDEVKRGQVLAKPGSITPHKKFKANIYVLKKEE
GGRHTPFTKGYRPQFYIRTADVTGELVDLPEGVEMVMPGDNVVMTVELIYPVAIEKGMRFAVREGGRTVGAGVVSEIIE
>Mature_398_residues
AKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKALAYKGFADFTPFDAIDKAPEEKARGITINVTHVEYETEKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAATDGVMPQTREHVLLARQVNVPAMVVFINKVDMVDDEELVELVEEEVRDLLSKY
EFPGDEVPVIKGSALMALEADNPDDPWVQKIYELMDAVDNYVPEPQRETDKPFLMPIEDIFSITGRGTVVTGRIERGVIH
VGDEVEIVGLSYEVRKTVVTGVEMFRKLLDEGVAGDNVGCLLRGVGKDEVKRGQVLAKPGSITPHKKFKANIYVLKKEEG
GRHTPFTKGYRPQFYIRTADVTGELVDLPEGVEMVMPGDNVVMTVELIYPVAIEKGMRFAVREGGRTVGAGVVSEIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=55.1637279596977, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=29.9334811529933, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=30.5803571428571, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=30.7174887892377, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=30.7174887892377, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=28.6163522012579, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI194018522, Length=363, Percent_Identity=28.3746556473829, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=363, Percent_Identity=28.3746556473829, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI194018520, Length=363, Percent_Identity=28.3746556473829, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI46094014, Length=363, Percent_Identity=27.8236914600551, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4503507, Length=271, Percent_Identity=26.9372693726937, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=130, Percent_Identity=33.8461538461538, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=399, Percent_Identity=70.1754385964912, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=70.3517587939699, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI2367247, Length=344, Percent_Identity=29.6511627906977, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=28.4848484848485, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994988, Length=255, Percent_Identity=31.3725490196078, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=149, Percent_Identity=33.5570469798658, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=51.7326732673267, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=48.2051282051282, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17552884, Length=458, Percent_Identity=30.1310043668122, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=458, Percent_Identity=30.1310043668122, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=30.0230946882217, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=443, Percent_Identity=28.8939051918736, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=443, Percent_Identity=28.8939051918736, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=27.1523178807947, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=309, Percent_Identity=27.5080906148867, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=156, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=29.0598290598291, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=25.6505576208178, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17565438, Length=339, Percent_Identity=26.5486725663717, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=135, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.8241206030151, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=30.9576837416481, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=30.9576837416481, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=22.6872246696035, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=444, Percent_Identity=27.9279279279279, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=25.9259259259259, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=33.1460674157303, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=27.1966527196653, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=57.5757575757576, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=57.5757575757576, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=48.8431876606684, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=30.8533916849015, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=30.8533916849015, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=30.8533916849015, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=30.8533916849015, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=30.7692307692308, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=30.7692307692308, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=29.5302013422819, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=29.7385620915033, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=450, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647046, Length=433, Percent_Identity=23.3256351039261, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647048, Length=433, Percent_Identity=23.3256351039261, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.9756097560976, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=242, Percent_Identity=28.5123966942149, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_KOSOT (C5CGR6)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941589.1
- GeneID:   7968395
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1904
- ProtClustDB:   PRK00049
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44099; Mature: 43968

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKALAYKGFADFTPFDAIDKAPEEKARG
CCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCC
ITINVTHVEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAATDGVMPQTREHV
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPAMVVFINKVDMVDDEELVELVEEEVRDLLSKYEFPGDEVPVIKGSALMALE
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
ADNPDDPWVQKIYELMDAVDNYVPEPQRETDKPFLMPIEDIFSITGRGTVVTGRIERGVI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEEEEEECCCEE
HVGDEVEIVGLSYEVRKTVVTGVEMFRKLLDEGVAGDNVGCLLRGVGKDEVKRGQVLAKP
EECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECC
GSITPHKKFKANIYVLKKEEGGRHTPFTKGYRPQFYIRTADVTGELVDLPEGVEMVMPGD
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCEEEECCC
NVVMTVELIYPVAIEKGMRFAVREGGRTVGAGVVSEIIE
CEEEEEEEEEHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKALAYKGFADFTPFDAIDKAPEEKARG
CCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCC
ITINVTHVEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAATDGVMPQTREHV
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPAMVVFINKVDMVDDEELVELVEEEVRDLLSKYEFPGDEVPVIKGSALMALE
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
ADNPDDPWVQKIYELMDAVDNYVPEPQRETDKPFLMPIEDIFSITGRGTVVTGRIERGVI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEEEEEECCCEE
HVGDEVEIVGLSYEVRKTVVTGVEMFRKLLDEGVAGDNVGCLLRGVGKDEVKRGQVLAKP
EECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECC
GSITPHKKFKANIYVLKKEEGGRHTPFTKGYRPQFYIRTADVTGELVDLPEGVEMVMPGD
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCEEEECCC
NVVMTVELIYPVAIEKGMRFAVREGGRTVGAGVVSEIIE
CEEEEEEEEEHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA